유전자재조합식품의 알레르기 항원성 평가

고미선 ( Mi Seon Ko ),윤소원 ( So Won Yoon ),오재원 ( Jae Won Oh ),이하백 ( Ha Baik Lee ) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 2008 소아알레르기 및 호흡기학회지 Vol.18 No.4

목적: 본 연구의 목적은 식품알레르기환자에서 유전자재조합식품(genetically modified organic food, GMO)이 알레르기항원성을 나타내는지 식품 알레르기 환자의 혈청을 검사하여 알레르기성을 평가하고자 하였다. 또한, 유전자재조합식품 중 표적 단백질을 식품 알레르기 환자의 혈청을 이용하여 추적 관찰하고 유전자재조합식품 알레르기성 평가에 대한 개선 방안을 검토하고자 하였다. 방법: 유전자재조합식품의 표적 단백질을 규명하고 알레르기성을 평가하기 위해 유전자재조합 식품 중 대두콩의 CP4 EPSPS와 옥수수의 Cry1f, Cry1Ab와 Pat 등 유전자를 클로닝 하였고, 이들 표적 단백의 단백 발현 유도를 위하여 형질 전환시켜 단백을 생산하였다. 대두콩과 옥수수에 대한 알레르기 양성 혈청과 음성 혈청으로 구분하여 SDS-PAGE 및 Western blotting한 후에 알레르기성을 판정하였다. 결과: 본 연구에서 4가지 표적 단백에 대한 알레르기성은 없었으며, 비특이적인 띠가 나타나기는 하였지만 이는 다른 단백에서도 나타나는 것으로 알레르기 특이성과는 무관한 것으로 사료된다. 또한, 각 표적 단백에 대해 DNA 서열을 분석하여 발견된 단백이 맞는지 확인한 결과 100%의 일치율을 보였다. 결론: 유전자재조합식품의 알레르기성 평가방법은 본 연구에서처럼 정제된 단백을 알레르기환자의 혈청을 이용하여 알레르기성을 평가할 수 있으나 향후 현실적으로 적용하는데 주의를 기울여야할 것으로 사료된다. Purposes: The aim of this study was to clarify whether genetically modified organic (GMO) foods cause any allergic reactions in patients with food allergy, by means of their serum tests. In addition, it was also attempted to perform follow-up observation on targeting proteins contained in GMO food, using the serum of patients with food allergy, and to show the improvement for evaluating GMO food as allergens. Methods: To identify the targeting proteins in GMO food and to evaluate their allergenic risks, several genes including CP4 EPSPS in genetically modified soybean, and Cry1f, Cry1Ab and Pat in genetically modified corn were cloned. The genes were transformed to synthesize proteins to induce protein expression of their target genes. The serums were divided allergy-positive and allergy-negative to soybean and corn, and SDS-PAGE and Western blotting were conducted, and finally allergenic risks were evaluated. Results: This study showed that the allergenic risks of 4 targeting proteins were insignificant. Although some non-specific bands appeared, it was considered that they were not associated with allergenic risk as they often appeared in other proteins. Additionally, as a result of analyzing DNA sequences for each targeting protein with the intention of protein identification, they perfectly matched. Conclusion: As a way to evaluate the allergenic risk of GMO food, it is reasonable to use the purified serum proteins of allergic patients as performed in this study. However, this evaluation method is carefully applied to the future practice. [Pediatr Allergy Respir Dis (Korea) 2008;18:292-304]

목뼈안정화운동이 전방머리자세 대학생의 머리뼈척추각, 깊은목굽힘근 근지구력과, 뒤통수근 긴장도 미치는 영향

고미선(Mi-Seon Ko),이진욱(Jin-Wook Lee) 한국컴퓨터정보학회 2023 한국컴퓨터정보학회 학술발표논문집 Vol.31 No.2

기초자치단체별 급성기 뇌졸중 사망률과 지역사회 위험요인 분석

고미선(Mi Seon Ko),이준영(Jun Young Lee),박홍준(Hong Jun Park),정재흥(Jae Hung Jung) 한국보건사회학회 2022 보건과 사회과학 Vol.- No.60

노인인구에서 발생률이 높은 뇌졸중 질환은 우리나라의 주요 사망원인이며 초고령사회에 접어든 우리나라의 주요한 보건 문제로 인식되고 있다. 이에 본 연구는 급성기 뇌졸중 사망률의지역별 영향요인을 파악하여 지역사회 건강 결과 향상에 도움이 되고자 하였다. 2017년 1월 1일부터 2019년 12월 31일까지 건강보험심사평가원의 급성기뇌졸중 적정성 평가자료를 활용하여 뇌졸중으로 진단되어 입원 후 30일 이내에 사망한 환자를 대상으로 기초자치 단체별 사망률을 분석하고 인구학적 (고령인구 비율, 남녀성비, 음주율, 흡연율, 주관적 건강수준 인지율, 스트레스 인지율), 사회경제적 (노후주택 비율, 재정자주도, 의료급여 수급자 비율), 의료 자원 (병원 수, 의료기관 종사 의사 수) 요인을 비교 분석하였다. 전국 226개 기초자치 단체의 급성기 뇌졸중 사망률은 12.1 ± 2.2 %였으며 사망률이 가장낮은 지역은 11%로 경기도, 사망률이 높은 지역은 충청북도와 전라남도로 각각 14.0%, 13.2% 로 관찰되었다. 사망률 상위 10개 기초자치단체는 모두 지방 소도시 (군 단위) 기초자치단체였다. 사망률에 영향을 미치는 요인으로는 전국 기초자치단체 분석 결과 고령인구비율 (β=0.326, p=0.001) 이었으며 대도시 권역 기초자치단체 분석의 경우에는 남녀성비 (β=-0.352, p=0.001), 음주율 (β=0.232, p=0.003), 노후주택비율 (β=0.354, p=0.001), 의료급여 수급자비율 (β=0.312, p=0.018), 병원 수 (β-0.177, p=0.027)가 통계학적으로 유의하게 관찰되었다. 지방 소도시 기초자치단체 분석 결과 고령인구 비율 (β=0.252, p=0.049), 재정자주도 (β =-0.166, p=0.014)가 사망률에 유의한 영향을 주는 요인으로 확인되었다. 급성기 뇌졸중 사망률은 지방소도시 기초자치단체에서 높게 관찰되었으며 인구학적 요인 외에도 지역의 사회경제적 요인 (재정자주도)과의 연관성을 확인하였다. 따라서 지역 수준에 따라맞춤형 보건 의료 정책을 수립하고 해결책을 모색할 필요가 있을 것으로 생각된다. We aim to elucidate the discrepancies in acute stroke mortality among primary local governments and identify the risk factors of the mortality at loco-regional level to establish tailored health policies in Korea. From January 1, 2017 to December 31, 2019, the mortality rates of patients who died from stroke within 30 days of hospitalization in 226 municipalities were analyzed using the claims data accumulated by Health Insurance Review and Assessment Service. Demographic factors (elderly population ratio, gender ratio, alcohol consumption rate, smoking rate, subjective health status awareness), socioeconomic factors (ratio of old houses, financial autonomy rate, rate of medical aid beneficiaries) and medical resource factors (number of hospitals/ doctors) were analyzed. Acute stroke mortality rate in Korea is 12.1 ± 2.2%. The region with the lowest mortality rate is Gyeonggi Province (local government mainly consisting of urban cities) and regions with the highest mortality rates are North Chungcheong Province and South Jeolla. The elderly population is associated with mortality using overall nationwide data. It was also risk factor for stroke mortality in the cities or counties in rural areas. The male-female ratio, alcohol consumption rate, financial autonomy rate, and number of hospitals in the region were also mortality risk factors based on the data of urban cities. Based on the results from counties, the proportion of elderly population and financial autonomy rate were found to be a significant risk factor for stroke mortality in small towns in rural areas. Regional differences of acute stroke mortality were also found. Aside from demographic factors (proportion of elderly population), socioeconomic factors (financial autonomy rate) were identified as risk factors for acute stroke mortality. Collectively, it is necessary to build health care policies that address the health inequality at the loco-regional level.

좁은 치조골에서 사다리꼴형 디자인으로 개발된 단폭경임플란트의 증례 보고

이사야(Sa Ya Lee),고미선(Mi-Seon Goh),고석영(Seok-Yeong Ko),윤정호(Jeong-Ho Yun) 대한치과의사협회 2018 대한치과의사협회지 Vol.56 No.5

Long-term survival and prognosis of narrow-diameter implants have been reported to be adequate to consider them a safe method for treating a deficient alveolar ridge. The objective of this study was to perform case report of narrow-diameter implants with a trapezoid-shape in anterior teeth alveolar bone. A 50-year-old male patient presented with discomfort due to mobility of all of the maxillary teeth and mandibular incisors. Due to destruction of alveolar bone, four anterior mandibular teeth were extracted. Soft tissue healing was allowed for approximately 3 months after the extraction, and a new design of implant placement was planned for the mandibular incisor area, followed by clinical and radiological evaluation. Implant placement was determined using an R2GATE surgical stent. The stability of the implants was assessed by ISQ measurements at the first and second implant surgery and after prosthetic placement. At 1 and 3 months and 1 year after implantation of the prosthesis, clinical and radiological examinations were performed. Another 50-year-old male patient presented with discomfort due to mobility of the mandibular central incisors. For the same reason as in the first patient, implant placement was carried out in the same way after extraction. ISQ measurements and clinical and radiological examinations were performed as in the previous case. In these two clinical cases, 12 months of follow-up revealed that the implant remained stable without inflammation or additional bone loss, and there was no discomfort to the patient. In conclusion, computer-guided implant surgery was used to place an implant in an optimal position considering the upper prosthesis. A new design of a narrow-diameter implant with a trapezoid-shape into anterior mandibular alveolar bone is a less invasive treatment method and is based on the contour of the deficient alveolar ridge. Through all of these procedures, we were able to reduce the number of traumas during surgery, reduce the operation time and total treatment period, and provide patients with more comfortable treatment.