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프로젝트 과정품질이 프로젝트 성과에 미치는 영향 : 시스템통합 프로젝트를 중심으로
고덕성,박소현,김승철 한국IT서비스학회 2019 한국IT서비스학회지 Vol.18 No.2
These days, many companies use information systems as a basis for organizational management activities and as an important component of sustainable management. Thus, securing and operating a high-quality information system that can improve the efficiency of business processes is a large part of corporate competitiveness. In order to get such a critical information system, it is only possible to successfully carry out the project. Various attempts and studies have been undertaken to increase the success rate of the project. However, the reality is that the success rate is still not getting much better. In this study, the concept of quality of service, which has long been actively studied in marketing area, was applied as a new factor to enhance the project's success. In this study, project performance activities were defined as the act of providing services. A survey of 164 valid collection data was analyzed. The analysis used SPSS 23. Four process quality factors (customer participation, customer response, knowledge and skills, reliability) and 21 metrics were derived. Through this research, I hope to be the starting point of applying process quality concept as a new breakthrough to increase success rate of project.
Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF)의 첨가가 생쥐 수정란의 발생과 착상관련 유전자 발현에 미치는 영향
김동훈,고덕성,이회창,이호준,강희규,김태전,박원일,김세웅,Kim, Dong-Hoon,Ko, Duck-Sung,Lee, Hoi-Chang,Lee, Ho-Joon,Kang, Hee-Gyoo,Kim, Tai-Jeon,Park, Won-Il,Kim, Seung-Samuel 대한생식의학회 2002 Clinical and Experimental Reproductive Medicine Vol.29 No.2
Objective : The purpose of the current series of experiments were to assess the effect of GM-CSF, as a medium supplement, on the development of mouse embryos and the expression of LIF and IL-1? mRNA. Materials and Methods: Mouse 2-cell embryos were collected from the oviducts of 6 weeks old ICR mice at 48 hours after hCG injection. Embryos were cultured in P-1 medium supplemented with mouse GM-CSF (0, 1, 5, 10 ng/ml). The embryo development to blastocysts and hatching blastocysts was assessed and the cell number in blastocyst was also examined. Using RT-PCR, the expressions of LIF and IL-1? mRNA in blastocyst were evaluated in the GM-CSF supplemented group and control group. Results: In mouse, the addition of GM-CSF increased the percentage of blastocysts (65.5%, 68.6%, 73.0% and 76.1% for control and 1, 5 and 10 ng/ml, respectively), and increased the proportion of hatching blastocysts (35.2%, 36.4%, 43.2% and 53.0% for control and 1, 5 and 10 ng/ml, respectively). The mean cell numbers in blastocyst were significantly increased in GM-CSF supplemented groups compared to control group. LIF and IL-1? expression in blastocyst were significantly higher in GM-CSF supplemented group than in control group. Conclusion: The results of experiment by mouse embryos showed beneficial effects of GM-CSF as a medium supplement. Furthermore, the addition of GM-CSF significantly increased the expression of LIF and IL-1? in mouse embryos. These results suggest that GM-CSF might be a important molecule in embryo implantation.
자궁 근종과 정상 자궁근층에서 Exon 5 가 결손된 에스트로겐 수용체 mRNA 의 발현 양상
박철범,박원일,고덕성,이호준 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.4
목적: 에스트로겐은 여러 에스트로겐 의존형 종양에서 그 성장에 관여하며, 정상 조직과 에스트로겐 의존성 종양에 다양한 종류의 에스트로겐 수용체가 존재한다는 실험결과가 기존에 많이 나와 있다. 최근에는 Exon 5 결손 에스트로겐 수용체 (D5-ER)가 많은 에스트로겐 의존형 종양에서 발견되었다. 본 연구에서는 자궁 근종과 D5-ER사이의 상호관계를 규명하고자 하는 것이 목적이다. 연구방법: 12명의 자궁 근종 환자와 12명의 대조군 에서 얻은 자궁근육층과 자궁 근종 조직에서 RT-PCR 을 이용해 D5-ER mRNA 와 wild type 에스트로겐 수용체(WT) mRNA의 상대적인 양을 측정하였다. WT/D5-ER 비율과 나이와의 상관관계도 측정하여 보았다. 결과: WT/D5-ER 의 값은 자궁근종 환자의 정상 자궁근층에서는 6.81±1.18 자궁근종에서는 6.40±1.20 으로 나타났고 대조군의 자궁 근층에서는 6.57±0.91로 나타나서 대조 군과 자궁근종 군, 자궁근종과 정상 자궁근층 사이에 통계학적 차이는 나타나지 않았다. 정상 자궁 근층내의 WT/D5-ER값과 나이의 상관관계를 조사하였으나 두 값 사이의 상관관계는 발견할 수 없었다 그러나 자궁 근종 조직내의 WT/D5-ER과 나이와의 상관관계를 조사해 본 결과 Rs= -0.789, p=0.002로 음의 상관관계를 나타내었다 결론: D5-ER은 대부분의 여성에서 흔히 일어나는 돌연변이이며 이는 자궁 근종의 생성과는 관계가 없는 것으로 여겨진다. 그러나 자궁근종내의 D5-ER은 자궁 근종의 성장과 관련이 있을 가능성이 있다. Objective: There is a large and increasing body of experimental and clinical data supporting the existence of variable estrogen receptors in both normal and neoplastic estrogen target tissue. Recently, exon 5 deleted estrogen receptor(D5-ER) has been identified in many estrogen dependent tumors. The purpose of this study is to assess the relationship between uterine myoma and D5-ER. Methods: The relative amount of wild type estrogen receptor mRNA and D5-ER mRNA were examined in human myometrium and myoma tissue obtained from 12 myoma patients and 12 control subjects by RT-PCR. The correlation between WT/D5-ER ratio and age was also evaluated. Results: There was no difference in expression of D5-ER mRNA not only between myoma and myometrim of the myoma patients, but also between myometrium of myoma patients and normal subjects. No significant correlation was found between D5-ER expression and age of the subjects in myometrial tissue. However, we could find negative correlation between WT/D5-ER ratio and age in myoma tissue. Conclusion: Upon these data, we conclude that D5-ER is a common variant mutation of estrogen receptor, which is not related with development of myoma. However, in myoma tissue, there is possibility that D5-ER may act some role in the growth of tumor.
The Regulators of VEGF Expression in Mouse Ovaries
신소영,이호정,고덕성,이회창,박원일 연세대학교의과대학 2005 Yonsei medical journal Vol.46 No.5
The objectives of this study were to explore whether ovarian vascular endothelial growth factor (VEGF) expression in mice can be regulated by IL-6 (interleukin-6), angiotensin II, FSH, and hCG; and to test whether the mouse ovarian VEGF expression can result in angiogenesis. The ICR mice were sacrificed, and their ovaries were recovered. Recovered ovaries were treated with IL-6, angiotensin II, FSH, and hCG separately and incubated for 24 hours in α-MEM. Expression of mRNA and protein of VEGF were assessed by RT-PCR and immunohistochemistry. The resulting angiogenesis was evaluated through immunohistochemical analysis for CD34. Treatment of mice ovaries with IL-6, FSH, and hCG resulted in a significant increase of VEGF mRNA, and IL-6 was the most potent inducer of VEGF. IL-6 and FSH resulted in increased neovascularization in the follicular phase of mouse ovaries. In contrast, angiotensin II could not increase VEGF expression or neovascularization. We documented an in vitro increase in VEGF expression by IL-6, FSH, and hCG; and reaffirmed that the proliferative response of murine ovarian endothelial cells paralleled an increase of VEGF expression.