기내$\gamma$-선 처리에 의한 감(Diospyros kaki Thunb.) 돌연변이 유기

고갑천 한국식물생명공학회 2000 식물생명공학회지 Vol.27 No.2

This study was carried out to know the optimum dose of gamma-ray for the induction of mutation in vitro and the characteristics of the mutants induced by gamma-ray in persimmon (Diospyros kaki Thunb.). The LD50 (50% lethal dose) for in vitro shoots of the cultivar, Nishimurawase was between 1 krad and 2 krad and about 1 krad for the cultivar, Ichikikeijiro. As the dose of gamma-ray increased, the length of shoots decreased and necrosis of buds increased. For the cultivar, Nishmurawase, 37.5∼58.3% shoots rooted and the rooting rate and the number of roots per shoot was low in high gamma-ray. The irradiated young plants which were grown in the growth cabinet for 6 weeks were shorter in shoot length and had more branches than non-irradiated plants. The survival rate of irradiated plants grown in the green house for 3 months was 33%, while 77% for control plants. 감 돌연변이 획득을 위해 기내배양 신초에 ${\gamma}$-선을 조사하여 변이유기를 위한 적정 선량, ${\gamma}$-선 조사 식물체의 기내 발근 및 생육 특성을 조사한 결과는 다음과 같다. 감 돌연변이 유기를 위한 적정한 조사 선량 (LD$_{50}$)은 서촌조생 품종에서 1 krad와 2 krad사이이었고, 일목계차랑 품종은 1 krad이었다. ${\gamma}$-선을 조사한 신초를 1차,2차, 3차에 걸쳐 계대배양하는 각 기간의 기내 생육상태를 보면 ${\gamma}$-선량이 증가할수록 기내에서 생장한 신초의 길이가 감소하였으며, 신초의 정아가 가장 장애를 많이 받았다. 서촌조생 품종에서 신초의 37.5~58.3% 정도가 발근되었고 발근율은 2 krad 처리에서는 가장 낮았다. ${\gamma}$-선 조사선량이 높아지면 발근율과 뿌리의 발생수가 줄어들었다. 순화한 뒤 6주 동안 생장상에서 생육한 ${\gamma}$-선 조사 개체는 무처리 개체의 묘고가 6.0 cm인데 비해 2krad 처리개체는 평균 묘고는 2.6 cm에 불과하였다. 또한 발생한 가지의 수도 무처리에서는 1.0개인데 비해 2krad에서는 1.8 개로 많았다. 온실 생육시에서 ${\gamma}$-선 처리선량이 높았던 개체는 처리되지 않았거나 낮았던 개체에 비해 묘고가 작고 분지수가 많았으며, 생육상태도 부진하여 생육 중에 33%정도만 생존하고 나머지는 고사하였다.다.

한글 초성자음 활용 방법의 개발

고갑천 호남대학교 1999 호남대학교 학술논문집 Vol.20 No.2

A methodology of using the first consonant character of korean language for operation of various instruments was developed. Adding the first consonant character of Hangul on numeric buttons of various electric products such as phone dialing, door key and remote controller, we can use them more conveniently then before. First, instead of using old fashioned system of dialing number to find company or institute, we can simply use the first consonant character(ㄱ, ㄴ, ㄷ, ...., ㅎ) of Hangul character. We can also convert a numeric telephone number into meaningful words or phrase, adding vowel or vowel and consonants to the first consonant characters properly. Then we can memorize the converted words or phrase instead of memorizing telephone number. Second, we can extend telephone functions in phone number memory, ARS or beeper system. Third, we can also apply this methodology to door key system which unlock the door by accepting numeric password. With this method, we can unlock the door by pressing the first consonant of passwords which used to numbers. This methodology will make our life better, as it is easy to apply this new system to various electric instruments such as air conditioner, TV, video player, etc., which all have numeric control system to. Thus, all of the products with the first consonants of Hangul can be controlled by the first consonant pressing system which presses the first consonant of words or phrase meaning their functions.

감마선 처리에 의한 정원장미 돌연변이 유기

고갑천,한태호,기광연 全南大學校 農業科學技術硏究所 2015 農業科學技術硏究 Vol.50 No.-

Garden rose popularity has been increased in Korea. However, the commercial demand has not reached out the development of domestic cultivar, and mainly imported cultivars are dominating the domestic market. In this work, mutant garden rose has been developed by using γ-ray irradiation. Rose ‘Love’ and ‘Rosanna’ cultivars were irradiated till 70Gy of γ-ray irradiation, and mutated into the form of flower color and shape. For three years, mutants for flower color and shape were selected and isolated into individual plantlets. Rose ‘Love’ with red flower color allowed five outstanding mutants focusing on various flower color and petal number. Rose ‘Rosanna’ also produced five excellent mutants with various flower color, shape, size and petal number.

器內 ??-線 處理에 의한 배 突然變異 誘起 및 選拔

高甲千 호남대학교 산업기술연구소 1999 산업기술연구논문집 Vol.7 No.-

배 器內生育 중인 新稍에 ??-선을 처리하여 돌연변이를 유기하고 여기서 黑斑病 抵抗性 變異體를 선발하는 시험을 실시하였다. 적정 ??-선 처리 線量(LD??)은 新水와 二十世紀 품종 공히 6krad는 과다하고, 4krad는 약간 낮은 감이 있었다. γ-선 처리선량이 높아지면 신초 및 腋芽의 증식 배수가 줄어들었다. 기내 생육 일을 이용한 흑반병 저항성 조기 검정법으로서, 菌絲배양에 의해 얻어진 독소액으로 검정하면 반응이 없었으나, 균사를 접종하면 병반이 발생했다. 기내신초를 마사토에 삽목하여 발근하면 기내에서 발근하는 것보다 發根率이 더 높아졌으며, 높은 감마선 선량이 照射된 신초는 발근율이 낮았다. 포장에서 생육한 ??-선 처리 신수 품종의 나무는 접목묘에 비해 樹高와 신초 발생수에 있어 차이가 있었으나 이십세기 품종은 차이를 보이지 않았다. 신수 품종 11계통 중 2계통과 이십세기 품종 22계통 중 1계통이 실내독소 검정과 포장의 생육 잎의 관찰에서 저항성으로 나타났다. In vitro shoots were irradiated with gamma rays to induce mutants resistant to pear black spot disease(Alternaria kikuchiana Tanka) from oriental pear cultivars, Shinsui and Nijisseiki. ??(50% lethal dose) of in vitro shoots was about 5 krad between 4 and 6 krad in two tested cultivars. In vitro shoots obtained after 4∼5 subcultures were more well rooted in ex vitro than in vitro. Rooting rate of shoots decreased in the treatment of gamma-ray of high dose. For the early test of resistance to pear black spot disease, AK toxin inoculation on in vitro leaf was not desirable for little response, but the inoculation of hypha on in vitro leaf has a possibility as it developed necrotic spots. In vitro irradiated trees grown for 2 years in the field showed shorter in the plant height and more branching than non-irradiated trees. After grown in the field for 2 years, AK toxin test of leaf and observations of leaf at the field showed that 2 lines of Shinsui and 1 line of Nijisseiki were resistant to black spot disease.

새로운 생장점 채취방법의 개발

고갑천 호남대학교 2000 호남대학교 학술논문집 Vol.21 No.2

A new method of excising meristems of plants was developed. By this new method, meristems can be excised from the base of shoot tips, but not from upper direction of shoot tips. Comparison between a usual method and a new method in excising meristems of oriental pears and apples showed that this new method was even much in number of excised meristems per hour, lower in loss percentage of materials and higher in survival rate after culture than a usual one. In horticultural crops, such as carnation, strawberry, and persimmon, meristems also could be excised more effectively in this new method than a usual method.

한글 초성자음 활용 방법의 개발

고갑천 湖南大學校 情報通信硏究所 1999 정보통신연구 Vol.9 No.-

A methodology of using the first consonant character of korean language for operation of various instruments was developed. Adding the first consonant character of Hangul on numeric buttons of various electric products such as phone dialing, door key and remote controller, we can use them more conveniently then before. First, instead of using old fashioned system of dialing number to find company or institute, we can simply use the first consonant character(ㄱ, ㄴ, ㄷ, ..., ㅎ) of Hangul character. We can also convert a numeric telephone numer into meaningful words or phase, adding vowel or vowel and consonants to the first consonant characters properly. Then we can memorize the converted words or phrase instead of memorizing telephone number. Second, we can extend telephone functions in phone number memory, ARS or beeper system. Third, we can also apply this methodology to door key system which unlock the door by accepting numeric password. With this method, we can unlock the door by pressing the first consonant of passwords which used to number. This methodology will make our life better, as it is easy to apply this new system to various electric instruments such as air confitioner, TV, video player, etc., which all have numeric control system to. Thus, all of the products with the first consonants of Hangul can be controlled by the first consonant pressing system which presses the first consonant of words or phrase meaning their functions.

배 기내 신초의 증식과 기내 및 기외 발근

고갑천,안규빈 호남대학교 2001 호남대학교 학술논문집 Vol.22 No.2

Meristems(0.6-0.9mm) obtained from shoot tips of pear were cultured on MS media containing various concentrations of BA to induce multiple shoots and multiplicated shoot was rooted in vitro and ex vitro using some kinds of methods. About 50% inoculated meristems were survived in the first culture. Medium containing 1∼2mg/L BA as a growth regulator was the most suitable for multiplication of in vitro shoots. Rooting was poor in vitro, while ex vitro rooting were effective when irrigating 2ml/L concentration EM solution. Rooted shoots grew up to about 90cm during one growing season.

배 기내 증식 배지의 질소원 종류별 신초 생육

고갑천 호남대학교 2005 호남대학교 학술논문집 Vol.26 No.2

This study was carried out to know the optimum concentration of NH_(4)NO_(3) and KNO_(3) added MS media for the culture of Oriental pear (Pyrus pyrifolia) in vitro. Meristems (0.6-0.9mm) obtained from axillary bud of pear were cultured on MS media containing 3mg/L BA for establishing shoots in vitro. The initial culture of meristem on the solid media was better than that on the liquid media at the point of view of shooting and vitrification of culture. The best result was obtained from the cultures in the MS media containing 20mM NH_(4)NO_(3) and 20mM KNO_(3) as the nitrogen sources. Fresh weight and shoot length obtained were highest after 5 week culture in the media containing 20mM NH_(4)NO_(3) and 20mM KNO_(3) in the culture of Oriental pear in vitro.

경단 절편 배양에 의한 바나나 급속 증식

고갑천 호남대학교 산업기술연구소 2000 산업기술연구논문집 Vol.8 No.-

바나나의 흡아에서 채취한 정단, 경단의 1/2절편, 그리고 1/9절편을 배양한 결과 BA를 5mg/ℓ를 첨가한M 바나나 흡아의 경단을 2등분 한 것, 6등분한 것, 그리고 경정을 BA가 다른 농도별로 첨가된 MS배지에 배양하여 효과적인 multiple shoot를 유기하기 위한 방법을 개발하였다. 부정아가 9등분한 경단절편에서 가장 많이 형성되었으며 이들 부정아는 엽초와 엽초사이 조직에서 발생되었다. 경단 절편배양에서 배지에 첨가된 BA 농도가 5mg/ℓ일 때 경단 절편으로부터 발생한 부정아의 수가 절편당 6.3개로 가장 많았으며 부정아의 생장도 빨랐다. 발생한 부정아로부터 얻어진 신초는 계대배양에 의해 증식할 수 있었으며, 계대배양 회수가 늘어남에 따라 배양한 신초당 다시 발생한 부정아 및 신초의 수는 더 많아졌다. 발근된 묘는 순화후 온실에서 2개월 뒤에 30∼40cm 크기로 생장했다. The shoot tips, and 1/2 and 1/9 shoot apex segments obtained from suckers of banana were cultured on MS media containing 0.0, 0.5, 1.0, 3.0, 5.0 and 7.0mg/ℓ BA, respectively, to induce multiple adventitious shoots in vitro. 6.2 adventitious shoots per segment were developed from 1/9 shoot apex segment when cultured on MS media containing 5mg/ℓ BA for 4 weeks. The number of developed adventitious buds and their growth were best on MS medium added with 5.0mg/ℓ BA. By progressing subculture, the number of developed shoots per explant increased gradually. Rooted plantlets grew up to 30∼40cm in 2 months in the plastic house.

Characteristics of Triploid Seedless ‘Mudeungsan’ Watermelon(Citrullus vulgaris) Compared to Its Diploid and Tetraploid Counterparts

고갑천 한국원예학회 2004 Horticulture, Environment, and Biotechnology Vol.45 No.6

This study was conducted to compare the growth and fruit characteristics of diploidy (2N = 22), triploidy (3N = 33), and tetraploidy (4N = 44) ‘Mudeungsan’ watermelon (Citrullus vulgaris). Seedlings and rooted cuttings of diploid grew earlier than those of triploid and tetraploid in the early growth stage. The higher the ploidy level, the larger the plant organs in general. The conspicuous differences among diploid, triploid, and tetraploid were on leaf length, width, area, and chlorophyll content, and tendril size. The DNA content per cell measured by flow-cytometry was 1.5- and 2-times higher in triploid and in tetraploid, respectively, than that in diploid. Diploid bore the first male flower at lower node than triploid and tetraploid. There was only a little difference in fruit set between diploid selfings and triploid pollinated with diploid. Fruit set of tetraploid was very low at 12.5% when selfed, while when pollinated with diploid it was comparatively high at 60%. The width/length ratio of fruits from diploid selfings, from triploid pollinated with diploid, and from tetraploid pollinated with diploid was 0.84, 0.92, and 1.02, respectively. The self-pollinated tetraploid fruits were mostly malformed and relatively small. The fruits from diploid selfings contained about 380 seeds per fruit, while those from triploid pollinated with diploid contained about 162 embryoless, but tough seed coats.