[의생활] 강황 에탄올 추출물의 염색과 응용

강샘,이정순 한국생활과학회 2016 한국생활과학회 학술대회 Vol.2016 No.5

강황 추출물을 함유한 PVA 나노섬유 제조

강샘,이정순 한국생활과학회 2016 한국생활과학회 학술대회 Vol.2016 No.5

강황 추출물의 기능성 성분과 강황 추출물을 함유하는 PVA 나노섬유의 제조

강샘,이정순 한국섬유공학회 2019 한국섬유공학회지 Vol.56 No.4

The purpose of this study was to analyze the functional ingredients of Curcumae longae Rhizoma extracts and to confirm the possibility of producing polyvinyl alcohol (PVA) nanofibers using these extracts. Distilled water and ethanol were used as extraction solvents. Terpene and its analogues as well as various volatile and essential oil components were detected in the distilled water and ethanol extracts. The total polyphenol content and flavonoid content showed that the Curcumae longae Rhizoma ethanol extract was higher than distilled water extract at 242.33 μg/ml and 166.65 μg/ml, respectively. PVA nanofibers containing Curcumae longae Rhizoma ethanol extract were electrospun in a relatively uniform manner without the occurrence of beads as a whole. As the content of Curcumae longae Rhizoma ethanol extract increased, the diameter of the fiber increased as well; the range of approximately 330 to 610 nm was added with a 0.25−1.5% addition. The hydrogen bonding between the Curcumae longae Rhizoma extracts and PVA, as well as the resulting degrees of crystallinity, melting point, and increase in heat capacity were identified through a Fourier-transform infrared spectroscopy, X-ray diffraction, and differential scanning calorimetry analysis. Therefore, it was verified that the developed Curcumae longae Rhizoma/PVA nanofiber was created in a state wherein the bond between the molecules were smoothly achieved.

음주 수준에 따른 여자 대학생의 임신 중 음주 인식 비교: 계획된 행위 이론을 적용하여

강샘이,김혜원 한국간호과학회 2019 Journal of Korean Academy of Nursing Vol.49 No.6

Purpose: The purpose of this study was to examine the awareness of alcohol use during pregnancy and factors influencing the intention to abstain from alcohol during pregnancy by drinking levels. Methods: Utilizing a comparative descriptive design, a total of 359 female college students were recruited. Measurements were Theory of Planned Behavior (TPB) variables related awareness of alcohol use during pregnancy, Korean Version of the Alcohol Use Disorders Identification Test, knowledge about alcohol use during pregnancy, and socio-demographic factors. Main variables were compared by drinking levels. The factors influencing the intention to abstain from alcohol during pregnancy were identified using hierarchical regression analysis. Results: Non-problematic alcohol behavior group (n=185) had higher the intention to abstain from alcohol during pregnancy than problematic alcohol behavior group (n=174) (t=3.13, p=.002). The significant TPB variables influencing the intention to abstain from alcohol during pregnancy were that attitudes (β =-.44, p<.001), subjective norms (β =.14, p=.026), and perceived behavior control (β =.19, p=.002) in non-problematic alcohol behavior group, whereas the corresponding variables in the problematic alcohol behavior group were attitudes (β =-.51, p<.001) and subjective norms (β =.21, p=.006). Conclusion: It is necessary to increase the intention to abstain from alcohol during pregnancy for the female college students having problematic alcohol behavior. The following strategies are recommended for them; decreasing acceptable attitudes toward alcohol use during pregnancy and reinforcing the influence of social pressure toward to abstain from alcohol during pregnancy.

최근 4년간 여성건강간호학회지에 게재된 여성건강 관련 연구의 동향(2013~2017년)

이영진,김서연,강샘이,강유정,김란,정희연,김혜원 한국여성건강간호학회 2018 여성건강간호학회지 Vol.24 No.1

Purpose: To analyze articles published in the Korean Journal of Women Health Nursing from 2013 to 2017 to determine the latest research trends and understand how 2013 Korea Women’s Health Statistics were reflected in journal articles. Methods: A total of 130 studies were analyzed. Research design, types of research, research framework, research subjects, characteristics of quantitative research, characteristics of qualitative research, and keywords were analyzed using a structured analysis format. Results: Quantitative and qualitative research accounted for 83.8% and 13% of these 130 studies analyzed, respectively. Non-experimental and experimental research accounted for 70.7% and 13.1% of these studies, respectively. The most frequent study subjects were childbearing women (62.8%), including college students, mothers, and adults. A total of 69.1% of non-experimental research and 88.2% of experimental research used convenience sampling. Questionnaires were most frequently used for data collection. The most frequent keyword domain involved health-related concepts (41%) among nine domains and the most frequently used keyword was “women.” Conclusion: This study suggest that further experimental research should be conducted in the future. Also, adolescent and the elderly women should be focused on as subjects in future studies based on results of 2013 Korean Women's Health Statistics.

강황 에탄올 추출물의 염색성

강샘(Sam Kang),이정순(Jung Soon Lee) 한국생활과학회 2015 한국생활과학회 학술대회 Vol.2015 No.11

추출 용매에 따른 강황 색소의 염색성 및 기능성

강샘(Kang, Sam),이정순(Lee, Jung Soon) 한국생활과학회 2017 한국생활과학회지 Vol.26 No.1

급성 저산소성 허혈성 뇌손상이 유발된 신생자돈에서 재산소-재관류기 동안 NG-monomethyl-L-arginine과 L-arginine이 뇌의 혈역학 및 에너지 대사에 미치는 영향

고선영,강샘,장윤실,박은애,박원순 대한소아청소년과학회 2006 Clinical and Experimental Pediatrics (CEP) Vol.49 No.3

Purpose : This study was carried out to elucidate the effects of nitric oxide synthase(NOS) inhibitor, NG-monomethyl-L-arginine(L-NMMA) and nitric oxide precursor, L-arginine(L-Arg) on cerebral hemodynamics and energy metabolism during reoxygenation-reperfusion(RR) after hypoxia- ischemia(HI) in newborn piglets. Methods : Twenty-eight newborn piglets were divided into 4 groups; Sham normal control(NC), experimental control(EC), L-NMMA(HI & RR with L-NMMA), and L-Arg(HI & RR with L-Arg) groups. HI was induced by occlusion of bilateral common carotid arteries and simultaneously breathing with 8 percent oxygen for 30 mins, and followed RR by release of carotid occlusion and normoxic ventilation for one hour. All groups were monitored with cerebral hemodynamics and cytochrome aa3 (Cyt aa3) using near infrared spectroscopy(NIRS). Na+, K+-ATPase activity, lipid peroxidation products, and tissue high energy phosphate levels were determined biochemically in the cerebral cortex. Results : In experimental groups, mean arterial blood pressure, PaO2, and pH decreased, and base excess and blood lactate level increased after HI compared to NC group(P<0.05). These variables subsequently returned to baseline after RR except pH. There were no differences among the experimental groups. In NIRS, oxidized hemoglobin(HbO2) decreased and hemoglobin(Hb) increased during HI(P<0.05) but returned to base line immediately after RR; 40 min after RR, the HbO2 had decreased significantly compared to NC group(P<0.05). Changes of Cyt aa3 decreased significantly compared to NC after HI and recovered at the end of the experiment. Significantly reduced cerebral cortical cell membrane Na+, K+-ATPase activity and increased lipid peroxidation products(P<0.05) were not improved with L-NMMA or L-Arg. Conclusion : These findings suggest that NO is not involved in the mechanism of HI and RR brain damage during the early acute phase of RR. 목 적 : 주산기 저산소성 허혈성 뇌손상의 병태 생리에서 nitric oxide(NO)가 급성 저산소성 허혈(hypoxia-ischemia, HI) 후 재산소-재관류기(reoxygenation-reperfusion, RR)에 대뇌의 혈역학 및 에너지 대사에 미치는 영향을 규명하기 위하여, NO 합성 억제제인 NG-monomethyl-L-arginine(L-NMMA)와 NO 합성 촉진제인 L-arginine(L-Arg) 투여를 통하여 뇌신경 세포에 어떠한 영향을 주는지 알아보고자 하였다. 방 법 : 생후 3일 이내의 신생자돈 28마리를 대상으로 무작위로 나누어, Sham 처치만 받은 정상 대조군(n=9), HI와 RR만 유발한 실험 대조군(n=7), HI 이후 RR 직전에 L-NMMA 투여군(n=6)과 L-arginine 투여군(n=6) 등 4군으로 구분하였다. 실험은 ether을 흡입 시킨 후 thiopental을 정주하고, 기관 삽관 후 인공호흡기 등의 처지를 끝낸 후, HI를 유발하기 위하여 실험군에서 수술 겸자로 양측 경동맥을 폐쇄한 후 8% 산소로 30 분간 흡입하였고, RR을 시행하기 위하여 경동맥 폐색을 풀고 흡입 산소농도를 60%로 올려 1시간까지 투여하면서 관찰하였다. 생리적 변수로 혈압과 동맥혈 가스 소견을 관찰하였고, 뇌의 혈역학적 변화와 에너지 상태는 near infrared spectroscopy (NIRS)를 이용하여 대뇌의 산화 헤모글로빈(HbO2), 환원헤모글로빈(Hb), 환산 헤모글로빈(HbD), 싸이토크롬 aa3(Cyt aa3) 등을 지속적으로 관찰하여 비교하였다. 또한 실험 종료 시 얻은 뇌조직에서 Na+, K+-ATPase의 활성도 및 지질 대사산물인 conjugated dienes, 고에너지 인분자인 ATP(adeninetriphosphate)와 phosphocreatine(PCr)을 비교하였다. 결 과 : 생리적 변수의 변화에서는 실험군 모두에서 정상 대조군에 비하여 혈압, 동맥혈 산소 분압, pH, base excess 등이 유의하게 감소하였고(P<0.05), 젖산은 유의하게 증가하였다(P< 0.05). L-NMMA와 L-Arg군에서 실험 대조군과 유의한 차이는 없었다. 실험군에서 RR 1시간 후 pH를 제외한 혈압, 동맥혈 산소 분압, base excess 등의 이상소견은 모두 기저치로 회복되었고, 실험군간에 유의한 차이가 없었다. NIRS 소견에서 HbO2와 HbD는 HI 동안 정상 대조군에 비하여 실험군 모두에서 유의하게 감소하였으나(P<0.05), RR 직후 기저치로 회복되었으며, HbO2는 RR 40분 이후 정상 대조군에 비해 유의하게 감소하였다(P<0.05). Hb은 정상 대조군을 제외한 모든 실험군에서 HI 동안 유의하게 증가하였다가(P<0.05), RR 직후 기저치로 회복되었다. 산화 Cyt aa3는 HI 동안 실험군 모두에서 감소하는 경향을 보였고, RR 이후 다시 증가하였다. 정상 대조군과 각 실험군간에 유의한 차이는 없었다. 뇌의 Na+, K+-ATPase 활성도와 conjugated dienes은 실험군 모두에서 정상 대조군(제1군)에 비하여 유의하게 감소하였다(P<0.05). 뇌의 ATP, phosphocreatine은 실험군 모두에서 정상 대조군과 차이가 없었고, 또한 실험군간에도 유의한 차이가 없었다.

PET/Cotton MVS 직물의 혼용률에 따른 드레이프성과 역학특성

최형열,김정화,강샘,이정순 한국감성과학회 2015 춘계학술대회 Vol.2015 No.-

드레이프성이란 직물이 자중에 의하여 아래로 처져서 주름이 발생하는 상태를 말하며, 드레이프의 형상은 의복에 따라 시각적 변형을 가져오게 하는데 관련된 성질로 제품의 착용감과 태에 밀접하게 관계되는 특성이다. 드레이프성을 평가하는 기술은 Cusick이 개발한 Cusick Drape Tester를 이용하여 드레이프 계수를 측정하고, 널리 보급되어있다. 하지만 장비를 사용한 수작업에 의한 정성적인 평가가 주로 이용되어 왔기에 본 연구에서는 화상입력장치를 활용하여 드레이프의 형상 인자 평가를 통한 정량적인 평가방법을 활용하여 실험을 진행했다. 실험에 사용한 시료의 특징으로는 MVS(Murata Vortex Spinning)방적사를 이용하여 혼용률을 달리하여 직물을 제직하여 진행했는데, MVS방적사는 가연을 통해 코어(Core)에는 꼬임이 없고 코어를 둘러싸고 있는 래핑(Wrapping)섬유의 꼬임에 의해 결속되어 있는 결속사의 형태를 가진다. MVS 방적사는 구조적으로 섬유전체가 회전하는 것이 아니라 내부섬유는 평행하고 래핑섬유는 회전하여 래핑섬유의 꼬임에 의해 결속 되어 있기 때문에 물성 특성에 영향을 미친다. 따라서 본 연구에서는 MVS방적사로 제직한 폴리에스터와 면 혼방 직물의 혼용률에 따른 역학적 특성과 이에 따른 기능특성치와 드레이프성과의 상관관계를 알아보았다.

태아 백서에서 임신 백서의 자궁 내 대동맥 결찰로 유발한 뇌실주위 백질연화증

장윤실,성동경,강샘,박수경,정유진,서현주,최서희,박원순 대한소아청소년과학회 2008 Clinical and Experimental Pediatrics (CEP) Vol.51 No.8

Purpose:This study was undertaken to develop an animal model of periventricular leukomalacia (PVL) induced by in utero clamping of pregnant rat aorta in fetal rats. Methods:A timed pregnanct Sprague-Dawley rat on embryonic day 21 just prior to delivery was sedated and anesthetized, and a Harvard ventilator for small animals was applied. Following laparotomy, the maternal aorta was clamped reversibly for 40 minutes using a surgical clip. The fetal rats were then delivered by Cesarean section, resuscitated if necessary, and reared by a surrogate mother rat until postnatal day 21 to obtain the brain specimen. After systemic perfusion and fixation, 10 µm thick serial brain sections were obtained and stained for pathologic examination and assessment of ventriculomegaly. Ventriculomegaly was assessed by the measured ventricle to total brain volume ratio. Results:Eight out of eleven fetal rats (73%) survived in the ischemia group after induction of in utero ischemia by clamping maternal rat aorta, and all ten survived in the control group. Body and brain weights measured at postnatal day 21 were significantly lower in the ischemia group compared to the control group. In pathologic findings, significant ventriculomagaly (3.67±1.21% vs. 0.23±0.06%) was observed in the ischemia group compared to the control group; although cystic lesion was not observed, mild (n=6) and moderate (n=2) rerefaction of the brain tissue was observed. Conclusion:A fetal rat model of PVL induced by in utero clamping of pregnant rat aorta was developed. (Korean J Pediatr 2008;51:874-878) Purpose:This study was undertaken to develop an animal model of periventricular leukomalacia (PVL) induced by in utero clamping of pregnant rat aorta in fetal rats. Methods:A timed pregnanct Sprague-Dawley rat on embryonic day 21 just prior to delivery was sedated and anesthetized, and a Harvard ventilator for small animals was applied. Following laparotomy, the maternal aorta was clamped reversibly for 40 minutes using a surgical clip. The fetal rats were then delivered by Cesarean section, resuscitated if necessary, and reared by a surrogate mother rat until postnatal day 21 to obtain the brain specimen. After systemic perfusion and fixation, 10 µm thick serial brain sections were obtained and stained for pathologic examination and assessment of ventriculomegaly. Ventriculomegaly was assessed by the measured ventricle to total brain volume ratio. Results:Eight out of eleven fetal rats (73%) survived in the ischemia group after induction of in utero ischemia by clamping maternal rat aorta, and all ten survived in the control group. Body and brain weights measured at postnatal day 21 were significantly lower in the ischemia group compared to the control group. In pathologic findings, significant ventriculomagaly (3.67±1.21% vs. 0.23±0.06%) was observed in the ischemia group compared to the control group; although cystic lesion was not observed, mild (n=6) and moderate (n=2) rerefaction of the brain tissue was observed. Conclusion:A fetal rat model of PVL induced by in utero clamping of pregnant rat aorta was developed. (Korean J Pediatr 2008;51:874-878)