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진생베리 아미노산 복합체로부터 분리한 펩타이드의 항염, 주름개선 효과
강상문 ( Sang Moon Kang ),박청 ( Chung Park ) 대한화장품학회 2019 대한화장품학회지 Vol.45 No.3
Ginseng berry(GB)는 진세노사이드Re를 함유하고 있으며, 항염, 피부 주름 완화의 기능을 가지고 있다. 본 연구에서는 Ginseng berry 아미노산 복합체의 TLC 분획 fraction 1, 2, 4를 확인하고 HPLC로 분석하였으며, fraction 1의 LC/MASS 분석을 통해 peptide (AP-1)를 동정하였다. AP-1에 의한 NO 생성 억제 효과를 조사하여 항염증 활성을 확인하였다. 또한 procollagen type I C-peptide (PIP) ELISA kit를 이용한 collagen 합성은 대조군 대비 50% 이상의 효과를 보였다. 이상의 결과로부터 진생베리 아미노산 복합체로부터 분리한 펩타이드는 항염과 주름개선 효능을 가진다고 사료되며, 향후 항염 및 항노화 화장품 원료로서의 이용 가능성을 보였다. Ginseng berry (GB) contains Ginsenoside Re and has anti-inflammatory and anti-wrinkle properties. In this study, TLC fractions 1, 2, and 4 of the ginseng berry amino acid complex were identified and analyzed by HPLC. And identified a peptide (AP-1) by LC/MASS analysis of fraction 1. The anti-inflammatory activity was confirmed by investigating the inhibitory effect of AP-1 on NO production. In addition, collagen synthesis using procollagen type I C-peptide (PIP) ELISA kit was 50% higher effective than that of the control group. From these results, the peptide isolated from ginseng berry amino acid complex is considered to have anti-inflammatory and anti-wrinkle effect, and may be useful as an anti-inflammatory and anti-aging cosmetic raw material.
Ye Seul Park(박예슬),Gun He Nam(남건희),Kyung Jo Jo(조경조),Hye Won Kawk(곽혜원),Je-Geun Yoo(유제근),Jin Dong Jang(장진동),Sang Moon Kang(강상문),Sang Yong Kim(김상용),Young Min Kim(김영민) 한국생물공학회 2019 KSBB Journal Vol.34 No.3
Collagen is decomposed by MMP-1, an enzyme induced by transcription factor activator protein 1 (AP-1) and matrix metalloproteinase-9 (MMP-9). Action of MMPs in inflammatory response promotes inflammatory cell movement and secretion, resulting in wrinkles on the skin. After using Bacillus sp. fermentation system and water, Artemisia vulgaris was fermented to prepare different solvent fractions using water, dichloromethane, hex ane, n-butanol, and ethyl acetate. These fractions were used to assess their effects on cell survival, wound healing, MMP-1/MMP-9 and procollagen type I C-peptide (PICP) expression, and skin turnover. MTT assay showed that cell viability of each treated group was 103% to 121%, indicating that A. vulgaris fractions were not toxic compared to control (cell viability: 100%). Wound healing assay revealed that wound healing ability in each treated group was 51% to 61%. This was lower than wound healing area in the control. Using RT-PCR, inhibition of MMP-1/MMP-9 gene expression was examined. As a result, each group treated with fraction showed reduced expression of both MMP-1 and MMP-9 compared to tumor necrosis factor-α (TNF-α) treatment group. Effects on collagen biosynthesis were analyzed using a PICP ELISA Kit. The group in which Artemisia vulgaris was treated increased collagen synthesis from 141 to 262ng/mL compared to the control group. Three-dimensional cell culture revealed that each fraction could increase skin wall formation. These results suggest that each fractions has anti-aging and anti-wrinkle effects on the skin, indicating its suitability as a functional material.
나이아신 펩타이드 융합체의 피부 미백 소재로서의 기능성 평가
김가연(Ga Yeon Kim),이승제(Seung Je Lee),전미지(Mi Ji Jeon),김보민(Bo Min Kim),김근태(Geun Tae Kim),강상문(Sang Moon Kang),이기영(Kee Young Lee),신은진(Eun Jin Shin),김상용(Sang Yong Kim),김영민(Young Min Kim) 한국생물공학회 2018 KSBB Journal Vol.33 No.1
The purpose of this study was to investigate the skin-whitening effects of Niacinamide-dipeptide Convergence (Niacin-peptide). To evaluate skin-whitening effects of Niacin-peptide, we performed tyrosinase inhibition assay and L-DOPA oxidation inhibition assay. These results demonstrated that Niacin-peptide decreased melanin production through tyrosinase inhibition. Also, we measured expression level of melanogenesis-related mRNA and proteins by reverse transcription-polymerase chain reaction (PCR) and western blotting. Results of PCR and Western blotting were showed that Niacin-peptide reduced expression of microphthalmia- associated transcription factor (MITF), tyrosinase related protein-1 (TRP-1), tyrosinase related protein-2 (TRP- 2) and tyrosinase. Thus, we identified the skin-whitening effect of Niacin-peptide and this finding demonstrated that Niacinamide-dipeptide Convergence may have potential as cosmetic ingredient.