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생쥐 난자 및 초기배아에서 Leptin 수용체 발현 및 Leptin에 의한 Mitogen Activated protein Kinase 활성의 조절 및 난자의 성숙 조절
강병문,한현주,서혜영,홍석호,계명찬,Kang, Byung-Moon,Han, Hyun-Joo,Seo, Hye-Young,Hong, Suk-Ho,Gye, Myung-Chan 대한생식의학회 2001 Clinical and Experimental Reproductive Medicine Vol.28 No.2
Objective: To verify the expression of leptin receptor (OB-R) in oocytes and preimplantation embryos, the involvement of mitogen activated protein kinase (MAPK or Erk1/2) in the leptin signaling, and effect of leptin on the oocyte maturation in mice. Method: RT-PCR analysis of OB-R was conducted in germinal vesicle (GV)-intact and MII stage oocytes, and 1, 2, 8-cell embryos and blastocysts. Germinal vesicle breakdown (GVB), polar body extrusion, monitored in the presence or absence of leptin ($1{\mu}M$). Following the leptin treatment, temporal changes in MAPK activity were verified by immunoprecipitation and in vitro kinase assay in MII oocytes. Results: The expression of OB-R mRNA was found in GV and MII oocyte but not in the embryos. MAPK activity of the MII oocytes was significantly increased by brief incubation in the HTF supplemented with leptin ($1{\mu}M$). Priming of PD098059, a MEK inhibitor to leptin treatment attenuated the activation of MAPK by leptin in MII oocytes. Following 24 hrs of culture of the GV oocytes, leptin significant increased the GVB and 1 st polar body extrusion. Conclusion: This result suggested that functional interaction between leptin and OB-R resulted in potentiation of MAPK (Erk1/2) activity in MII oocytes through MEK activation and that leptin might be a local regulator of meiotic maturation of the mouse oocytes.
난관복원술전에 실시한 불임검사의 임신율 예측지표로서의 유용성에 관한 연구
강병문,Kang, Byung-Moon 대한생식의학회 1993 Clinical and Experimental Reproductive Medicine Vol.20 No.1
Since the successful advent of IVF-ET, reproductive surgeons have been forced to compare pregnancy outcomes of surgical procedures for tubal infertility with those of IVF-ET. The current study was designed in an effort to determine the usefulness of pre-operative infertility work-up, especially diagnostic laparoscopy, in predicting the pregnancy rate and in assessing the reversal feasibility. 109 patients who underwent a microsurgical tubal reversal were reviewed. The patients were followed over one year. The total intrauterine pregnancy, spontaneous abortion, and ectopic pregnancy rates were 66.4%, 3.7%, and 7.3%, respectively. The result showed that the use of loupe might increase the chance of the ectopic pregnancy. I found that the final tubal length, especially the longest tubal length, the site of reanastomosis, and the methods of sterilization affected the pregnancy rates. A 85% intrauterine pregnancy rate was achieved in the patients with good preoperative assessments(Group II-1). There was only 10% incidence of intrauterine pregnancy in the patients with bad assessments(Group II-2). It is concluded that the pre-operative infertility work-up, especially diagnostic laparoscopy, is a good diagnostic tool in assessing the reversal feasibility.
강병문,이석원,채희동,강은희,추형식,김정훈,장윤석,남주현,Kang, Byung-Moon,Lee, Suk-Won,Chae, Hee-Dong,Kang, Eun-Hee,Chu, Hyung-Sik,Kim, Chung-Hoon,Chang, Yoon-Seok,Nam, Joo-Hyun 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.1
Since the blastocyst is broken and spreads out on a flat plastic culture dish (two dimensional culture) during in vitro development, it has been difficult to study the implantation process. It also has been difficult to analyse the interactions between endometrial epithelial and stromal cells because of the lack of a long-term in vitro model which can stimulate in vivo characteristics, as these cells eventually fail to proliferate or cease to express differentiated functions. Recently nontransformed cell lines, CUE-P and CUS-V2, derived from rat endometrial epithelium and stroma were reported. In this study, morphology of CUE-P and CUS-V2 was examined and oxytocin gene expression by CUE-P cells was demonstrated by RT-PCR. The CUE-P cells have a cuboidal morphology and CUS-V2 cells resemble fibroblast and exhibit a spindle-like morphology. In RT-PCR, same size of PCR products of oxytocin gene at hypothalamus, uterus and CUE-P cells were demonstrated. These results showed three dimensional culture system could be made by using the new cell lines.
배아밀도와 배양액 용량이 착상전후의 생쥐배아의 체외 성장에 미치는 영향
강병문,전용필,김지영,김정희,이지윤,채희동,김정훈,장윤석,목정은,Kang, Byung-Moon,Cheon, Yong-Pil,Kim, Ji-Young,Kim, Jeong-Hee,Lee, Ji-Yun,Chae, Hee-Dong,Kim, Chung-Hoon,Chang, Yoon-Seok,Mok, Jung-Eun 대한생식의학회 1997 Clinical and Experimental Reproductive Medicine Vol.24 No.3
The effects of embryo number and incubation volume on the development of mouse embryos were evaluated. The growth rate of two-cell mouse embryos to attached blastocyst stage and the growth rate of blastocysts to early somite stage were assessed after culture in different incubation volumes and embryo densities. Embryos were collected from ICR female mice superovulated with pregnant mare serum gonadotropin and human chorionic gonadotropin and mated by ICR males. In experiment 1, groups of one, five, ten, twenty 2-cell embryos were cultured in 10-, 50-, 500-, 1000-${\mu}l$ drops of BWW media under mineral oil at $37^{\circ}C$ in a humidified atmosphere of 5% $CO_{2}$ and 95% air. As the incubation volume decreased, significantly (p<0.05) higher rates of embryos reached morular and blastocyst stage on day 3 and 4 culture, respectively. In experiment 2, groups of one, five, ten, twenty blastocysts were cultured in 1- and 2-ml volumes of CMRL 1066 media under same condition as in experiment 1. However the reverse was the result. Decreasing the number of embryos incubated per volume from 1 to 20 significantly (p<0.05) increased the number of blastocysts reaching the late egg cylinder (LEC) and early somite (ES) stage on day 6 and 8 culture, respectively, regardless of incubation volume. Blastocysts cultured in 2ml had higher (p<0.05) development rates to LEC and ES stage on day 6 and 8 culture, respectively, than embryos cultured in 1ml. Our results suggest that the effects of embryo number and incubation volume on the development of mouse embryos are stage specific and the shifting point was between hatching and EEC stage.
Extracellular Matrix 배지에서 생쥐 배아의 발생 및 아폽토시스
강병문,손인표,정병목,최규완,계명찬,Kang, Byung-Moon,Sohn, In-Pyo,Chung, Byung-Mok,Choi, Kyoo-Wan,Gye, Myung-Chan 대한생식의학회 2000 Clinical and Experimental Reproductive Medicine Vol.27 No.3
Objective: To verify the effect of Matrigel, a ECM complex from Engelbreth-Holm-Swarm (EHS) mouse sarcoma on the preimplantation development and apoptosis of mouse fertilized eggs. Method: Late pronucleus stage eggs were cultured through the blastocyst stage in the presence of Matrigel (0.5%, v/v). Characteristics of apoptosis and cell number assesed by Hoecst staining and TUNEL labeling at the blastocyst stage, respectively. Results: Morphological development, number of cells per embryo was significantly increased but rate and number of TUNEL positive nuclei of the embryo were decreased in the presence of Matrigel. Conclusion: This result suggested that at low concentration of Matrigel improves both viability and morphological development in the preimplantation mouse embryos.
강병문(Byungmun Kang),김대은(DaeEun Kim) 대한전기학회 2021 대한전기학회 학술대회 논문집 Vol.2021 No.10
본 논문에서는 자기장 센서 기반으로 구성된 자기장 센싱 시스템을 이용하여 외부 자기장에 따른 위치 추정하기 위한 자기장 센서 보정 기법과 외부 자기 물체에 따른 자기장 변화를 이론적인 모델링을 소개한다. 자기장 센서의 정확한 보정 기법을 기반으로 외부 자기 물체의 위치에 따라 자기장 센서에 미치는 영향을 이론적으로 해석 할 수 있는 모델링을 통해서 자기장 센서 주변의 외부 자기 물체의 위치를 정확히 추정할 수 있다.