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Neocalimastix frontalis 로 조제한 fungal additive 가 반추위 발효에 미치는 영향
하종규,강민원,이상석 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.5
The anaerobic rumen fungus, N. frontalis N1-1, which had a high fiber-degrading capacity was selected to make a fungal additive and to evaluate its effects on ruminal fermentation. The fungal additive was added at the level of 0.03, 0.06, 0.09, and 0.12%(w/v) in Lowe's medium and pH, gas production, dry matter degradation, CMCase, xylanase, and volatile fatty acid(VFA) were determined after 2, 4, and 6 days of incubation. Addition of high level of fungal additive (0.09 and 0.12%) decreased pH compared with control treatment(P$lt;0.05). The gas production was increased by the addition of fungal additive. The highest increase in gas production was achieved with 0.09, and 0.1% supplementation after 6 days incubation(P$lt;0.05). The degradation of dry matter was increased with incubation time up to 6 days and was significantly lower in control. Activities of carboxymethylcellulase(CMCase), and xylanase in the culture supernatant was increased by the addition of fungal additive with highest increase after 4 days of incubation. Total VFA production and acetate:propionate ratio also tended to increase by fungal additive after 4 days of incubation similar to results of enzyme activities.
하종규,강민원,이상석 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.4
Five different anaerobic fungi (Neocallimastix frontalis N1-1, Piromyces communis P1-2, Orpinomyces spp. 02-2, Caecomyces communis CI-5, Anaeromyces spp. AI-3) isolated from the rumen of a male Holstein were compared in fermentation characteristics and enzyme activities in a hope to select a proper fungal species with a high fiber-degrading capacity. The fungal isolates were incubated in Lowe's medium containing rice straw as a substrate for 5 days, and pH, gas production, volatile fatty acid concentration, and activities of carboxymethylcellulase(CMCase), xylanase and amylase in the culture supernatant were determined. Culture supernatant of N-1 had the lowest pH, and monocentric generally showed lower pH than polycentric fungi. N-1 and P-I produced more gas than the other fungal isolates. Overall gas production data indicated that it could be used as a potential indicator of fungal growth. The concentration of acetic acid in the culture fluid of N1-1 and A1-3 was significantly higher than that of the other fungal isolates, and isobutylate was detected only in the culture of 02-2. All of five fungal species produced CMCase, xylanase and amylase, and Nl-I showed the highest enzmye activity. Based on data obtained in this study, Neocallimastix frontalis showed the highest fiber digesting ability among 5 fungal species.