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      • KCI등재

        Acaricidal and insecticidal responses of Cinnamomum cassia oils and main constituents

        강민승,이회선 한국응용생명화학회 2018 Applied Biological Chemistry (Appl Biol Chem) Vol.61 No.6

        Insecticidal and acaricidal responses of Cinnamomum cassia oils made by organic solvent (OS), steam distillation (SD), and supercritical fluid (SF) and their components were examined in two bioassays (contact and fumigant bioassays) against Plodia interpunctella, Sitophilus oryzae, S. zeamais, Tyrophagus putrescentiae, and Sitotroga cerealella adults. Using the contact or fumigant bioassay against T. putrescentiae adults, OS oil exhibited the strongest toxicities (50% lethal dose [LD50], 2.60 lg/ cm2 and 1.34 lg/cm3), followed by SF and SD oils. Furthermore, using two bioassays, SD oil against S. oryzae and S. zeamais adults exhibited the strongest toxicities (LD50, 102.25 lg/cm2 and 68.62 lg/cm3, 102.03 lg/cm2 and 57.59 lg/cm3), followed by SF and OS oils. Using the fumigant bioassay against S. cerealella and P. interpunctella adults, OS oil exhibited the strongest toxicities (LD50, 1.17 lg/cm3 and 0.79 lg/cm3) followed by SF and SD oils. Cinnamaldehyde, cinnamyl acetate, and coumarin against T. putrescentiae adults showed no significant differences in the contact bioassay, but in the fumigant bioassay, cinnamaldehyde exhibited the highest toxicity (LD50, 0.91 lg/cm3) followed by cinnamyl acetate and coumarin. Against S. oryzae, S. zeamais, S. cerealella, and P. interpunctella adults, cinnamaldehyde using two bioassays exhibited the most potent toxicities (LD50, 108.81 lg/ cm2 and 77.80 lg/cm3, 104.72 lg/cm2 and 36.48 lg/cm3, 0.57 lg/cm2 and 2.29 lg/cm3), followed by coumarin and cinnamyl acetate in order. The results showed that cinnamaldehyde and the C. cassia oils could be effective values in the management of stored product pests.

      • KCI등재

        Cloning and Characterization of the Tetracycline Resistant Gene, tetB, from Vibrio parahaemolyticus

        강민승,박큰바위,황혜진,배향남,임근식,엄성환,김영목 한국수산과학회 2009 Fisheries and Aquatic Sciences Vol.12 No.1

        A tetracycline resistant Vibrio parahaemolyticus, capable of growing on TCBS medium containing tetracycline, was isolated from cultivated fishes. A gene responsible for the tetracycline resistance was cloned from chromosomal DNA of the V. parahaemolyticus strain using Escherichia coli KAM3, which lacks major multi-drug efflux pumps (△acrB) as host cells. The nucleotide sequence and homology analysis revealed an open reading frame (ORF) for tetracycline resistance protein (TetB). In order to characterize the antibiotic resistance of TetB originated from the V. parahaemolyticus strain, the gene was subcloned into plasmid pSTV28. The resulting plasmid was designated as pSTVTetB and transformated into E. coli KAM3. E. coli KAM3 cells harboring the recombinant plasmid pSTVTetB are able to grow on plates containing tetracycline and oxytetracycline but not doxycycline, indicating that the tetB gene confers the tetracycline- and oxytetracycline-resistance to the host cell.

      • KCI등재

        이전 이식편 후부각막실질 전체가 남은 상태에서 시행된 이차 데스메막박리내피각막이식술

        강민승,이지은 대한안과학회 2021 대한안과학회지 Vol.62 No.1

        Purpose: To report well-attached cornea after secondary Descemet’s membrane stripping automated endothelial keratoplasty (DSAEK) without removing the previous graft. Case summary: A 72-year-old man was referred for decreased visual acuity due to bullous keratopathy in the right eye. He had a history of cataract surgery and trabeculectomy in both eyes and primary DSAEK in the right eye 2 years ago. A new DSAEK graft was superimposed onto the previous grafted posterior stroma due to difficulties with primary graft removal. The patient was laid in supine position for 6 hours to facilitate graft attachment. Grafted cornea remained clear and well-attached for 3 months postoperatively. Conclusions: When previous grafts were difficult to remove completely, new graft could be superimposed onto the previously grafted posterior stroma. 목적: 데스메막박리자동내피각막이식(descemet stripping automated endothelial keratoplasty, DSEAK) 시행 과정 중 이전 이식된각막편의 제거가 완전하게 되지 않은 채 시행된 DSAEK에서 이식된 공여각막이 잘 유지되었던 증례를 보고하고자 한다. 증례요약: 72세 남자 환자가 우안 수포각막병증 소견으로 각막이식을 위해 의뢰되었다. 양안 백내장 및 섬유주절제술을 받은 적 있으며, 2년 전 우안 DSEAK 병력이 있었다. DSAEK시 이전에 이식되어 있던 각막이식편을 제거하려고 하였으나, 후부각막실질의 제거가용이하지 않아 이를 남겨둔 채 새로운 공여각막을 전방에 주입한 뒤 무균공기를 주입하여 각막을 유착시켰다. 술 후 6시간 앙와위자세를 유지하였으며, 항생제 및 스테로이드 안약을 점안하면서 경과 관찰하였다. 술 후 3개월의 경과 관찰 기간 동안 각막 투명도는잘 유지되었으며, 각막단층촬영검사상 이식된 각막편은 잘 유착된 상태로 관찰되었다. 결론: 이전 각막이식편의 완전한 제거가 힘들 경우 이전 이식편 후부각막실질을 그대로 둔 채 DSAEK을 시행하더라도 합병증 없이공여각막의 유착을 기대할 수 있을 것으로 사료된다.

      • KCI등재

        포스트 코로나 시대 소비가치와 항공사 선택속성이 항공사 선택에 미치는 영향*: 다항로짓모형 적용

        강민승 (사)한국관광레저학회 2022 관광레저연구 Vol.34 No.10

        In this study, a multinomial logit model was applied by identifying consumption values and airline selection attributes, suggesting domestic airlines preferred by users, and confirming airline selection factors. In the case of the Korean Air choice group, preference was high for male, education level and office workers. Positive (+) results were derived from 'additional services' and 'convenience and safety', but the influence of 'social value orientation' of consumption value factors did not appear. In the case of the Asiana Airlines choice group, among consumption value factors, ‘social value orientation’ had a negative (-) effect. In the case of the low-cost airline selection group, female preferred, and a positive (+) influence was confirmed in 'social value orientation'. Airline selection attribute factors had a negative (-) effect on ‘additional services’, ‘convenience and safety’ and a positive (+) influence on ‘cost rationality’ was confirmed.

      • KCI등재

        전신마취수술 이후 발생한 양측 후대뇌동맥 폐쇄에 의한 피질맹 2예

        강민승,김수진 대한안과학회 2020 대한안과학회지 Vol.61 No.5

        Purpose: To report two rare cases of cortical blindness resulting from posterior cerebral artery (PCA) occlusion after laminectomy and laparoscopic gastrectomy under general anesthesia. Case summary: (Case 1) A 65-year-old man presented with visual loss after a bone graft and total laminectomy for spinal stenosis under general anesthesia. His best-corrected visual acuity (BCVA) was hand motion in both eyes. The pupillary light reflex was normal, and a relative afferent pupillary defect (RAPD) was not observed. Slit-lamp microscopy and fundus examination were normal. Using brain computed tomography, magnetic resonance imaging (MRI), and angiography, a PCA infarction was detected. His left 2b PCA was reperfused after intra-arterial thrombolysis. However. he still had left homonymous hemianopia and right homonymous superior quadrantanopia. (Case 2) A 64-year-old man was referred for visual loss in the recovery room after laparoscopic distal gastrectomy under general anesthesia. His BCVA was finger counting for the right eye and hand motion for the left eye. His pupillary light reflex was normal. A RAPD was not observed. Slit-lamp microscopy and a fundus examination were normal. Using a brain MRI and angiography, PCA infarctions were detected. Echocardiography showed an atherosclerotic change with a mobile plaque. Anticoagulation therapy was started, but he still had left homonymous hemianopia and right homonymous inferior quadrantanopia. Conclusions: Cortical blindness caused by non-ophthalmological surgery under general anesthesia is known for severe complications and poor prognoses. Therefore, it is crucial to identify patients with high risks of complications to prevent ophthalmic complications resulting in visual losses before surgery. 목적: 전신마취하 척추수술 및 위암수술 후 양측 후대뇌동맥 폐쇄에 의한 피질맹이 발생한 증례 2예를 보고하고자 한다. 증례요약: (증례 1) 65세 남자 환자가 전신마취하에 척추수술 후 4일째 시력저하가 발생하였다. 시력은 양안 안전수동이었으며, 대광반사, 상대구심동공운동장애, 세극등현미경, 안저검사는 정상이었다. 뇌 컴퓨터단층촬영 및 자기공명영상에서 후대뇌동맥 부위의 경색 소견이 확인되었다. 시야검사상 양안 동측성 좌측 반맹과 우측 상사분맹이 남았다. (증례 2) 64세 남자 환자가 전신마취하에 복강경 위절제술 시행 후 회복실에서 시력저하를 호소하였다. 시력은 우안 안전수지, 좌안 안전수동이었으며, 대광반사, 상대구심동공운동장애, 세극등현미경, 안저검사는 정상이었다. 뇌 자기공명영상에서 양측 후대뇌동맥 영역의 경색 소견이 확인되었다. 시야검사상 양안동측성 좌측 반맹과 우측 하사분맹으로 나타났으며, 추적 관찰 2년째에도 회복되지 않았다. 결론: 시력과 무관한 부위의 수술 후에 발생한 피질맹은 예측하기가 어렵고, 예후가 매우 불량하며 치명적인 합병증이다. 전신마취하수술 후 발생할 수 있는 시기능 저하의 위험 요인에 대한 연구와 이를 예방하기 위한 노력이 필요할 것이다.

      • KCI등재

        기내서비스품질의 만족도가 행동의도에 미치는 영향 연구: 대형항공사와 저가항공사의 조절효과를 중심으로

        강민승,이희찬 (사)한국관광레저학회 2017 관광레저연구 Vol.29 No.12

        In this study it is intended to propose differentiated and effective way to secure competitiveness in this severe airline market by analysing the influential relationship between satisfaction of in-flight service quality and customer's behavioral intention. The hierarchical regression analysis was carried out to review the difference of in-flight service quality factor to behavioral intention between Full-Service Carriers (FSC) and Low-Cost Carriers(LCC). For FSC factors tangibility and empathy gave significant and positive(+) influence, while for LCC factors tangibility and responsiveness gave significant influence. So there was difference between FSC and LCC. Like this, in this study the influence of the satisfaction on service quality factors, consisting in-flight service, of FSC and LCC to behavioral intention has been reviews, and this study has its meaning on providing useful direction for marketing differentiation by managing quality attribute of in-flight service.

      • KCI등재

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