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아토피피부염 동물 병태모델에서의 형개연교탕(荊芥連翹湯)의 면역조절작용
강란이 ( Ran Yi Kang ),박보경 ( Bo Kyoung Park ),김선빈 ( Seon Bin Gim ),최학주 ( Hak Joo Choi ),김동희 ( Dong Hee Kim ) 대전대학교 한의학연구소 2009 혜화의학회지 Vol.18 No.2
Atopic dermatitis induced NC/Nga mice were used to investigate the efficacy of HYT on the recovery of dermatitic symptoms by how HYT influenced the immune related factors. The results are as below: 1. Compared to the control, HYT treated group showed recovery of atopic dermatitis by the naked eye observation, and significant reduction of dermatits index was observed after 14 weeks. 2. HYT treated group showed significant decrease of the ratio of CCR3+, B220+/IgE+, and Gr-1+/CD11b+immune cells in dorsal skin by 40.5%, 34.2%, and 48.1%, respectively. 3. HYT treated group showed increase in the ratio of CD19+ immune cells within PBMC by 10.8%, as well as decrease in CD3+, CD3+/CD69+, NKT+ ratios by 5.3%, 35.2%, and 44.9%, respectively. 4. HYT treated group showed increase in the expression of IFN-γ in serum by 589.3%, whereas the expression of IL-4, IL-5, IL-6, IL-13, TNF-α, MCP-1 and RANTES were decreased by 31.4%, 82.1%, 97.1%, 39.5%, 83.7%, 26.1%, 48.6%, respectively. A 47.2% decrease in IgE expression was also observed. The results above strongly supported the improvement of atopic dermatitis by HYT treatment through immune modulation. Further studies on the synergistic effect of each ingredients of HYT and therapeutic effects according to the dosage of each ingredient should be followed for clinical applications. This work was (partly) supported by the RIC program of MKE(Ministry of Knowledge Economy) in Daejeon University.
애기땅빈대의 열수 추출 및 에탄올 추출물의 항산화 활성 비교
강란이 ( Ran Yi Kang ),최학주 ( Hak Joo Choi ),박지원 ( Ji Won Bak ),심부용 ( Boo Yong Sim ),이해진 ( Hae Jin Lee ),김동희 ( Dong Hee Kim ) 대전대학교 한의학연구소 2014 혜화의학회지 Vol.23 No.1
Objectives : In this study, the antioxidant activities of the 80% ethanol and hot water extracts of Euphorbia supina Rafinesque were investigated. Methods : We measured total phenol contents, flavonoid contents, 2,2-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, 2,2``-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay. The production of reactive oxygen species was measured in LPS-induced RAW 264.7 cells using flow cytometry system. Results : The content of phenol in the hot water extract was 65.529 ± 0.462 mg/g and 126.932 ± 1.894 mg/g in the 80% ethanol extract, and that of flavonoid in the hot water extract was 16.063 ± 0.471 mg/g and 29.159 ± 1.963 mg/g in the ethanol extract. The 80% ethanol extract also showed higher DPPH and ABTS radical scavenging activities (90.8±1.0% and 92.5±0.7%) than the hot water extract (81.5±0.5% and 91.5±0.2%). The production of reactive oxygen species(ROS) was reduced dose-dependently by 80% ethanol and hot water extract at concentration of 1, 10 and 100 ㎍/㎖of RAW 264.7 cells. Conclusion : According to these results, the 80% ethanol extract of Euphorbia supina Rafinesque has a good anti-oxidative effects than the hot water extract. Thus, the 80% ethanol extract of Euphorbia supina Rafinesque may serve as useful natural antioxidants.