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총담관 결찰후 집토끼 결장점막내 배상세포의 미세구조적 변화
고정식,강권철,양남길,안의태,박경호 순천향대학교 1991 논문집 Vol.14 No.2
담관이 폐쇄되었을 때 결장점막내 배상세포의 미세구조적 변화를 알아보기 위하여 체중 2㎏ 내외의 집토끼 총담관을 이중으로 결찰한 후 1일, 3일, 5일,7일 및 14일이 경과한 후, 결장원위부의 조직을 절취하였다. 절취된 조직은 전자현미경 관찰을 위하여 통산적 방법으로 고정, 탈수 및 포매하여 JEM 100CX Ⅱ전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1. 결장점막상피를 구성하는 세포중 점액을 분비하는 세포로는 장은와원주세포와 배상세포가 있다. 2. 수술후 3일군의 배상세포의 경우 점액과립중 전자밀도가 낮은 것들은 융합되어 있는 경우가 많았고, 과립형질내세망이 다소 확장되어 있었다. 3. 수술후 5일군에서는 미성숙 배상세포가 많이 관찰되었다. 4. 수술후 7일, 14일군의 배상세포는 점액과립의 생성과 분비양상이 정상세포의 것과 같아 보였다. 따라서 총담관이 결찰되면 초기에는 배상세포의 점액방출 및 생성이 억제되나 시일이 경과되면 배상세포의 분화가 활성화되어 점액의 부족분을 보완해 주어 정상상태로 회복되는 것 같다. This experiment was performed to study the morphological changes of the goblet cells in the rabbit colonic mucosa after common bile duct ligation. Healthy adult rabbits weighing about 2㎏ body weight were divided into normal and bile duct ligated groups. Common bile duct ligation was performed under ether anesthesia. Experimental animals were sacrificed on the 1st, 3rd, 5th, 7th and 14th day after the operation. Mucosal specimens from the distal colon were fixed in 2.5% glutaraldelyde-1.5% paraformaldehyde, followed by 1% osmium tetroxide, embedded in araldite mixture, cut with LHB-V ultratome, stained with uranyl acetate-lead citrate, and observed with JEM 100 CX Ⅱ electron microscope. Observed results were as follow: 1. There were two types of mucous secreting cells in the rabbit distal colon; crypt columnar cell and goblet cell. 2. In the 3rd day after bile duct ligation, there found electron lucent mucous granules in the goblet cell which were occasionally fused together, and moderately distended granular endoplasmic reticulum. 3. In the 5th day after operation, the rabbit colonic goblet cells are frequently seen immature type. 4. In the late stage of the experiments, the rabbit colonic goblet cells showed general findings of the mucous granules, and other cell organells were similar to normal ones. Considering the above findings, common bile duct ligation probably resulted the hypoformation and hyposecretion of mucous granules of goblet cells in the early stage, and may facilitate the differentiation of goblet cells in the late stage.
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원저(原著) : 공혈혈액의 B형간염 판독기준에 관한 연구
장동익 ( Dong Ik Chang ),강권철 ( Kwon Chul Kang ),한상무 ( Sang Moo Han ),이충영 ( Chung Young Lee ),조도현 ( Do Hyun Cho ) 대한임상병리사협회 1999 임상수혈검사학회 발표자료집 Vol.5 No.1
This study was aimed to develop a more accurate method for the detection of HBV(Hepatitis B Virus) infection in the donated sera at the Kyounggi Do Red Cross Blood Center. The samples were collected from February 1998 to August 1998. The sera were devided into 5 groups(A, B, C, D, and E) according to OD(Optical Density)value of EIA(Enzyme Immunoassay). EIA,RIA(Radio Immunoassay) and PCR(Polymerase Chain Reaction)were performed for these samples to compare the results. Among 10 samples of group A(cut off : 0.050~0.073). EIA for HBsAg was negative for the whole sample, but the result of RIA was found to be negative for 9 sample with one positive. The results of neutralization test were the same in EIA and RIA. The sera of group C which were equivocal by EIA were found to be negative by RIA. The group D(OD value : 0.500~l.000) showed 9 positive sera by EIA of HBsAg, but only one positive by RIA of HBsAg. For group E. the result of EIA was in perfect accordance with that of RIA except one serum. Late confirmation of this serum proved that the result of the first EIA was false positive. Dilution experiments showed that the sensitivity of PCR was 105~6 time higher than EIA. When the result of PCR-amplified HEV DNA detection was compared with those of EIA and RIA, the presence of HBV was confirmed even in group A. which group was judged safe by EIA and RIA. This result suggests that a more accurate method like PCR-amplification of HBV DNA should be developed for the safety of public health.
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