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질트리코모나스에 대한 마우스의 세포매개성 면역반응에 관한 연구
신명헌,민득영,류재숙,안명희,신수재 한양대학교 의과대학 1990 한양의대 학술지 Vol.10 No.1
This study was aimed to observed the cell-mediated response by duration after immunization with T. vaginalis in mice. Trichomonas vaginalis, isolated from vulvovaginitis patients, was cultured in the TPS-1 medium axenically. For the experiments trichomonads were inoculated subcutaneously with 1×10?? of trophozoites into the back of BALB/c mice. Following immunization, delayed type hypersensitivity (DTH) response in the footpad and blastogenic response of the mouse spleen cells using [³H]-thymidine were observed on week 1, 4 and 7 after immunization. In the preparation of T. vaginalis lystae, trophozoites wer disrupted with ultrasonicator. Suspension was sedimened, filtered and dialyzed. For the collection of T. vaginalis excretory-secretory product, trophozoites were suspended in RPMI 1640 medium and incubated at 37℃ for 3-4 hours. The supernatant was filtered and dialyzed. T. vaginalis lysate, excretory-secretroy product and PHA were used as mitogens in the blastogenic responses. ELISA technique was applied for the measurement of the serum antibody titer. The results obtained are as follows: 1. The blastogenic response of splenocytes to PHA was slightly decreased on week 4 after immunization, whereas those to T. vaginalis lysate and excretory-secretory product were similar with control group on same time course. Both of these blastogenesis were markedly increased on week 7 after immunization. 2. In regard to delayed type hypersensitivity responses in mice immunized with T. vaginalis, the levels were increased in comparison with the control group throughout 7 weeks after immunization. 3. The serum antibody titers in mice immunized with T. vaginalis were increased continuously from on week 1 to on week 8 after immunization. With these results it is assumed that not only humoral immunity but also cell mediated immunity is involved in immune defense mechanism in the mice experimentally inoculated with Trichomonas vaginalis.
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