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Poxvirus 感染에 있어서의 Virus宿主細胞의 相互關係
김우호(金宇鎬) 대한바이러스학회 1977 Journal of Bacteriology and Virology Vol.7 No.1
In a series of comparative studies on virus-host cell interaction of poxviruses, an analysis in swinepoxvirus-porcine kidney cell (Pky) system bv cytochemical and autoradiographic methods was performed. An established cell line of porcine kidney cells, Pky, at 320 subcultured level, were inoculated with PP-1 strain of swinepoxvirus. Characteristic cytopathic effects (CPE) which consist of rounded and refractile cells were produced on 4 5days postinoculation in the homologous Pky monolayers. Increasing of CPE number in the Pky monolayer culture occurred more slower than that of cowpoxvirus FL cell, and orf virus-monkey kidney or bovine kidney cell systems (heterologous). Cytochemical and autoradiographic treatments of cultured infected monolayers were performed together. In these preparations, compact or diffuse intracytoplasmic inclusions which correspond to the type-B inclusion were observed. The inclusions were developed to the less defined type by the infection was progressing. No intracytoplasmic inclusion which correspond to the type-A inclusion was observed. The use of low level of 'H-thymidine appears that to yield rather detailed content of the inclusion because of sparse deposits of silver grains. The accumulation of silver grains was coincided with the inclusions, thus it was concluded that these inclusions must be the site of viral DNA synthesis and virus maturation.
Epstein-Barr Virus(EBV) 와 사람의 腫瘍
김우호(金宇鎬) 대한바이러스학회 1976 Journal of Bacteriology and Virology Vol.6 No.1
Recently the evidence for an association between herpesviruses and neoplasia was rapidly accumulated. Herpesviruses are the only DNA viruses which have been associated with spontaneous malignancies in man. A herpesvirus has been well shown to be the epidemic cause of Marek's disease of fowl, and other herpesviruses also have been suspected or known to be oncogenic in man or other animals. Since the first observation of Epstein et al. (1964) of an unknown herpes-type virus, now referred to a Epstein-Barr virus (EBV), in cultured Burkitt lymphoma cells, the putative role of this virus in human carcinogenesis has been well documented. EBV appears.to meet several of the criteria, Burkitt lymphoma (BL), nasopharyngeal carcinoma (NPC) and infectious mononucleosis (IM), for an oncogenic virus. I believed that a comparative approach on diseases caused by oncogenic herpesviruses would be useful and of mutual value for the investigators. The current state of knowledge on EBV and associated laboratory techniques was briefly reviewed.
미국 방사선학회코드 (ACR code)의 자동 코드화 방식에 대한 검토
김우호 대한영상의학회 1990 대한영상의학회지 Vol.26 No.2
The authors developed a computer program for automation for the coding by ACR (American college of Radiology) code. The automatic coding can reduce radiologist's burden and make more accurate coding possible in most of the situation. This program used IBM PC AT and was written in dBASE Ⅲ plus language. We established the ACR dictionary as followings; procedure code='ACR 32', anatomy code='-761',pathology code='_312/7' The codes were automatically established while the results were typer written, Uncoded diagnosis was remained into 28 digits. All of the searching wee performed by index file, with which searching time is negligible regardless of the file size.