음성인식을 활용한 언어평가에 대한 이론적 고찰 및 연구

김봉규,김경신 21세기영어영문학회 2019 21세기영어영문학회 학술대회 Vol.2019 No.09

Dust Production of 9P/Tempel 1 after Deep Impact

김봉규,김승리,이충욱,문홍규,이동주,구재림,강용범 한국천문학회 2005 天文學會報 Vol.30 No.2

실버타운 모형개발에 관한 연구

김봉규 관광품질시스템학회 1997 관광품질시스템연구 Vol.3 No.1-2

The Study for old people has become going on the sociology, psychology and nursing area, however, the study of the structure of the residental space for them in not enough. In developed country, the study with the concept of Silver Town community, not a nursing camp(like an asylum), has been keeping on actively, but in Korea, there are few studies of developing the space where old people, who has the economic power and stable position in society and family, can live enjoying the rest of life, as the concept, that the welfare policy for old people would be to provide them with the facilities mainly for diet life and nursing comp, caused the studies to be concentrated with the tourism development. The unidentified concept for Silver Town(namely old people's community) was identified community for old people which is funded by healthy old people who are over 60, in charge with operating expense about the community and have economic independence; the community which, in special area, provides old people with residential facilities and the opportunity for relaxation with all programs for recreation, sports, culture and education for old people in order to live a comfortable life; the community which has medical facilities preparing against desease caused in old people's long stay and bank facilities for resident; the complex developed community(facility space) which has resort function area where old parents can get along with their decendants to creat vigorous residential space, not inactive space.

觀光호텔의 採用 및 配置管理에 관한 實證的 硏究

김봉규 관광품질시스템학회 1995 관광품질시스템연구 Vol.1 No.1

The purpose of this study is accomplished by developing the systems and models to be applied to the tourist hotel personnel management through the theoretical and empirical analysis. The following goals are developed for the purpose of this study. First, to develop the personnel management goal of the hotel management system analysing the theories on the tourist hotel personnel management. Second, to present the items on the effectiveness evaluation of the tourist hotel personnel management and improve the theoretical system on the personnel effectiveness evaluation. Third, to develop the new models of the effectiveness evaluation of the tourist hotel personnel management. Fourth, to evaluate the result of each personnel management through the empirical analysis on the luxurious tourist hotels.

英語 語彙의 分析比較 調査硏究 : 中學校 3學年 新舊 英語 敎科書

金奉圭 한국영어교육연구학회 1989 영어교육연구 Vol.- No.9

반도체 Advanced Packaging용 Photo-definable 절연막 상용화 기술 개발

김봉규,이종찬 한국공업화학회 2020 한국공업화학회 연구논문 초록집 Vol.2020 No.-

반도체 첨단 패키징은 다양한 전자 기기에 반도체를 적용하기 위해 반드시 필요한 제조 공정으로, 향후 5G, 모바일 AP, 자율주행차, IoT, 가전 등의 다양한 시스템 산업 급속한 성장에 맞춰, 반도체 제품의 시장 선점 및 안정적인 산업 성장을 위해 기술 경쟁력을 강화해야 한다. 반도체 Advanced package를 위해서는 포토레지스트, 현상액, 식각액, 박리액, 절연막, 도금액 등 다양한 공정 소재 및 기능 소재가 필요하지만, 아직까지 이 소재들의 대부분은 수입 소재, 특히 일본 소재에 의존하고 있는 것이 현실이다. 반도체 패키징 고객사의 경우 소재 미확보시 발생하는 제품생산 중단 및 소재 변경에 대한 최종 고객사의 보수적인 소재 변경 허가 등으로 인하여 소재 변경 불가하였으나, 최근 일본 수출 규제로 인한 글로벌 소재 공급망의 위험성 대두로 그간 개발 및 사업화가 어려웠던 advanced package용 소재들에 대한 국산화에 탄력을 받고 있다. 특히 상용화에 어려움을 겪었던 Photo-definable 절연막 소재의 기술, 산업 현황을 공유하고, 성공적인 개발 및 상용화를 위한 전략적인 접근법에 대해 이야기 하고자 한다.

대장균에서 naringenin으로부터 생리활성 isokaemferide의 생합성

김봉규 한국응용생명화학회 2019 Journal of Applied Biological Chemistry (J. Appl. Vol.62 No.1

The flavonoid, isokaempferide, has various biological activities such as hepatoprotective, antimicrobial and antiproliferative effect and is extracted from Amburana cearensis and Cirsium rivulare (Jacq.). Biotransformation is an alternative tool for the synthesis of value-added flavonoids with inexpensive substrates. Here, to synthesize isokaempferide from naringenin, two genes, PFLS and Rice O-mthyltransferae-9 were introduced in Escherichia coli. Although isokaempferide was successfully synthesized, the amount of biosynthesis was no high. In order to increase the yields of isokaempferide, S-adenosylmethionine (SAM) used as a methyl donor was increased by deleting MetJ, which is a transcriptional regulator related to SAM biosynthetic pathway. Next we optimized the cell concentration and substrate feed concentration with the engineered E. coli strain. Through these strategies, the biosynthesis of isokaempferide was increased up to 87 mg/L. Flavonoid인 isokaempferide는 간 보호, 항균, 항증식성, 항염 등다양한 생리활성을 가지는 것을 보고 되고 있으며 Amburana cearensis와 Cirsium rivulare (Jacq.)과 같은 식물에서 추출하여사용한다. 생물전환은 비교적 값이 싼 물질로부터 고부가가치물질을 얻기 위한 방법으로 유용물질의 식물추출법을 대체할 수있는 방법이다. Naringenin으로부터 isokaempferide를 생합성 하기 위해 대장균에 포플러에서 분리한 PFLS와 벼에서 분리한ROMT-9 유전자를 도입하였다. 이 균주를 이용하여 naringenin 으로부터 isokaempferide의 생합성 수율(9.8 mg/L)은 낮았다. Isokaempferide의 생합성 수율을 높이기 위해 S-adenosylmethionine (SAM) 생합성 경로의 transcriptional regulator인MetJ를 제거함으로써 methyl donor로 사용되는 SAM을 증가시켰다. SAM생합성 대사를 조절한 대장균을 이용하여 생물전환균주의 최적 세포밀도 및 최적의 기질공급 농도를 결정하였다. 최적화된 조건을 이용하여 isokaempferide의 생합성은 87 mg/L 까지 증가하였다.

포플러로부터 flavone synthase I 유전자의 클로닝 및 생화학적 특성

김봉규,안중훈 한국응용생명화학회 2009 Journal of Applied Biological Chemistry (J. Appl. Vol.52 No.1

포플러는 naringenin, kaempferol, myricetin, apigenin, luteolin, rhamnetin, quercetin과 같은 플라보노이드를 가지고 있다. 이러한 플라보노이드들은 여러 가지 효소에 의해 naringenin으로부터 합성된다. 그러나, 포플러에서는 플라보노이드 합성에 관여하는 효소들의 연구가 거의 이루어 지지 않았다. 포플러 에서 flavone synthase I 유전자를 RT-PCR방법을 이용하여 클로닝 하였다. PFNS I-1 유전자의 기질 특성을 알아보기 위하여 대장균에 과발현하여 glutathione S-transferase 친화 크로마토그래피를 이용하여 정제하였다. 정제한 PFNS I-1 재조합 단백질은 flavanone인 2S-naringenin기질을 이용하여 flavone인 apigenin을 생성함을 알 수 있었다. 또한 cofactor인 oxoglutarate, FeSO4, ascorbate, catalase가 PFNS I-1의 반응성에 중대한 영향을 미치는 것을 알 수 있었다. 이상의 결과를 종합해 볼 때 PFNS I-1 유전자는 flavone synthase I을 암호화하는 유전자임을 확인하였다. Poplar contains various flavonoids including naringenin, kaempferol, myricetin, apigenin, luteolin,rhamnetin, and quercetin. These flavonoids are synthesized from naringenin with various enzymes. However, none of genes from poplar involved in flavonoid biosynthesis have been biochemically characterized. We cloned PFNS I-1 from Populus deltoids by RT-PCR method. The open reading frame of PFNS I-1 consisted of 1,017-bp and it showed high similarity with other FNS genes. The purified recombinant PFNS I-1, expressed in Escherichia coli, catalyzed the reaction from flavanone (naringenin) to flavone (apigenin). The reaction of PFNS I-1 was enhanced by cofactors such as oxoglutarate, Fe2+, ascorbate and catalase. Thus, it is concluded that PFNS I-1 encodes a flavone synthase I.

Synthesis of PSPI with Enhanced Mechanical Properties

김봉규,박혜진,이상원,허완수,정명섭 한국고분자학회 2002 한국고분자학회 학술대회 연구논문 초록집 Vol.2002 No.4

관광호텔의 인터넷마케팅 활성화방안에 관한 연구 : 서울지역 특 1 급 관광호텔을 대상으로

김봉규 한국관광산업학회 2000 Tourism Research Vol.14 No.1

In recent years many a enterprise tends to establish its own homepage for the introduction of newly developed production such as effective marketing and customer management. Tourist hotels in Korea are also active in keeping with this global stream. The internet marketing through homepage is mostly focused on the hotel merchandise. In other words, this homepage marketing is not in keeping with the true purpose of internet marketing such as image improvement, market survey and customer satisfaction, etc. The study results are as follows; First, Lack of internet usage instruments such as -display use net and news group due to the over - reliance on website. Second, Lack of market survey and customer information management due to the over focusing on the production sales. Third, Lack of direct and active advertising for the hotel and hotel internet homepage due to the operation by internet agencies not by hotel personnels. Fourth, Lack of internet marketing specialist of hotel's own and absence of any independent department for this purpose. Fifth, Lack of obligation and response for the information circulation in the process of internet marketing. Numerous enterprise homepages appear and disappear continually every day. So even if any hotel establishes its own homepage it loses its value when customers do not visit it. Therefore continual advertising for the homepage and marketing activities to induce customers' visits and the prompt replacement of newly developed information are necessary.