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허영태,하우태,이란,이원영,정하연,황규찬,송혁 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.6
Objective: Glucose is an essential fuel in the energy metabolism and synthesis pathways of all mammalian cells. In lactating animals, glucose is the major precursor for lactose and is a substrate for the synthesis of milk proteins and fat in mammary secretory (alveolar) epithelial cells. However, clear utilization of glucose in mammary cells during lactogenesis is still unknown, due to the lack of in vitro analyzing models. Therefore, the objective of this study was to test the reliability of the mammary alveolar (MAC-T) cell as an in vitro study model for glucose metabolism and lactating system. Methods: Undifferentiated MAC-T cells were cultured in three types of Dulbecco's modified Eagle's medium with varying levels of glucose (no-glucose: 0 g/L, low-glucose: 1 g/L, and high-glucose: 4.5 g/L) for 8 d, after which differentiation to casein secretion was induced. Cell proliferation and expression levels of apoptotic genes, Insulin like growth factor-1 (IGF1) receptor, oxytocin receptor, αS1, αS2, and β casein genes were analyzed at 1, 2, 4, and 8 d after differentiation. Results: The proliferation of MAC-T cells with high-glucose treatment was seen to be significantly higher. Expression of apoptotic genes was not affected in any group. However, expression levels of the mammary development related gene (IGF1 receptor) and lactation related gene (oxytocin receptor) were significantly higher in the low-glucose group. Expressions of αS1-casein, αS2-casein, and β-casein were also higher in the low-glucose treated group as compared to that in the no-glucose and high-glucose groups. Conclusion: The results demonstrated that although a high-glucose environment increases cell proliferation in MAC-T cells, a low-glucose treatment to MAC-T cells induces higher expression of casein genes. Our results suggest that the MAC-T cells may be used as an in vitro model to analyze mammary cell development and lactation connected with precise biological effects.
사전보정기법을 사용한 새로운 지상파 DTV 대역외발사강도 측정법
허영태,권원현,Her, Young-Tae,Kwon, Won-Hyun 한국전기전자학회 2016 전기전자학회논문지 Vol.20 No.1
In this paper, out-of-band emission regulations and measurement methods of terrestrial DTV broadcasting services are analyzed, and a novel method to evaluate the out-of-band emission characteristics of DTV transmitter is proposed. Experimental results show that proposed method can be easily used to measure the characteristics of DTV transmitter with inexpensive measuring equipments. With a few modifications, proposed method can be widely adopted to measure the RF performances of the general radio equipments efficiently. 본 연구에서는 우리나라 DTV 방송의 대역외발사강도 기술기준 및 기존 측정방식의 문제점들을 분석하였으며, 지상파 DTV 방송의 대역외발사강도를 효과적으로 측정할 수 있는 방안들을 제안하였다. 고가의 전용 측정장비로만 측정가능한 기존방식에 비해 제안한 방식은 범용장비로도 대역외발사강도를 손쉽게 측정할 수 있으며, 실제 측정시스템 구현을 통하여 제안한 방식의 유효성을 입증하였다. 본 논문에서 제안된 방식은 경제적이고도 간편하므로 방송장비 이외의 무선 송신장치 특성측정에도 널리 사용될 수 있다.
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허영태,김동구,엄상준,Heo, Young-Tae,Kim, Dong-Gu,Uhm, Sang-jun 한국수정란이식학회 2018 한국동물생명공학회지 Vol.33 No.3
It has been claimed that artificial insemination (AI) of cows with frozen-thawed semen treated with commercially produced kits, Wholemom (in favour of female gender) increases the birth chance of calves with desired sex ratio by approximately 85% without decrease of pregnancy rates. Hence, this study was conducted to investigate the efficacy of wholemom kits as combined with frozen-thawed bovine semen during in vitro fertilization on the in vitro fertilization and developmental efficiency and sex ratios such as some reproductive parameters in bovine. For this, 1,737 oocytes were in vitro fertilized and developed. Agglutination effects on bovine after treatment of Wholemom kit were observed by time passage and dose respectively. To determine sex of embryos, Bovine embryo Y-specific gene primers(ConEY) and Bovine specific universal primer(ConBV) were used as multiple PCR method. Fertilization rate of wholemom-treated group was significantly lower than its of control group[66.9% (1,156/1,737) in Wholemom-treated group; 75.0% (610/813) in control group]. However, developmental rate after fertilization of both wholemom-treated and control groups were not significantly different [26.1% (404/1,156) in Wholemom-treated group; 27.4% (224/610) in control group]. Sex ratio of in vitro fertilized embryo with frozen-thawed semen treated with wholemom kit was determined by multi PCR. Female ratio in wholemom-treated group [85.4% (173/201)] was significantly higher than its of control group [47.2% (66/141)]. In conclusion, wholemom treatments of semen used in the in vitro fertilization and development of bovine oocytes provided increase in female ratio with decrease of fertilization rate.