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가지과식물에서 Capsidiol 생합성에 관여하는Cytochrome P450 유전자의 발현과 효소활성
권순태,Paul Hasegawa 한국자원식물학회 2008 한국자원식물학회지 Vol.21 No.2
Enzyme activity and expression of cytochrome P450 gene involved in the pathway of capsidiol biosynthesis were compared in five different solanaceae plants such as red pepper, green pepper, tobacco, potato and egg plant. Base on genomic DNA and/or RT-PCR results, four solanaceae plants such as red pepper, green pepper, tobacco and egg plant possess P450 gene in the genome and specifically expressed by elicitor treatment. However, potato was appeared to have neither P450 nor cyclase gene in the genome. P450 genes did not show any expression in the plants under normal condition, but showed highly specific expression under elicitation condition in various organs and tissue such as leaf, root, stem and culture cells.
오세명,정형진,권순태 安東大學校 農業科學技術硏究所 1997 農業科學技術 硏究論文集 Vol.4 No.-
야생더덕은 재배더덕에 비해 조섬유와 석유에텔추출물 함량 비율이 높았고 수분, 환원당 및 조단백질 함량은 낮았다. 더덕의 뿌리가 오래된 것일수록 조섬유, 환원당, 조회분 및 석유에텔성분의 함량이 높았고 수분과 조단백질은 낮았다. 항산화효소인 superoxide dismutase와 peroxidase의 활성은 더덕뿌리의 년수가 오래된 것일수록 높았고 야생더덕이 재배더덕에 비해 높았으나 catalase의 활성은 년근수가 적을수록 높았고 야생 및 재배더덕간에는 차이가 없었다. 야생더덕이 재배더덕에 비해 malic산의 함량이 현저히 낮았으나 타 성분은 큰 차이를 보이지 않았고, 년근이 오래된 것일수록 유기산의 함량이 증가하는 경향이었다. 지방산인 oleic, linoleic 및 palmitic 산은 년근이 오래된 것일수록 함량이 높았고 야생더덕이 더욱 높았다. Content of crude lipid, petroleum ether extracts and crude fiber were higher in wild grown species than in cultivated species, and that of water, reducing sugar and crude protein were vice versa. The older roots showed the higher contents of crude fiber, reducing sugar, crude ash and petroleum ether extracts. However, content of water and crude protein were higher in younger roots than in older roots. Wild grown species and/or older roots showed higher enzyme activity of superoxide dismutase and peroxidase than those of cultivated species and/or younger roots. Younger roots showed higher catalase activity than older roots. Wild species contained lower malic acid and citric acid contents than those of cultivated species tested. Content of fatty acids such as oleic, plamitic and linolenic acid were higher in wild species and/or older roots than cultivated species and/or ypunger roots. However, content of linoleic acid was lower in wild species.
권순태,전익조,Cody Marklez 한국자원식물학회 2014 한국자원식물학회지 Vol.27 No.1
Correlation and similarity of garlic cultivars was analyzed by measuring the composition of twenty amino acids contents in the bulb and callus from twelve garlic cultivars which were collected from Korea and foreign countries. Arginine and asparagine occupied more than 78% of total amino acids both in bulb and callus followed by glutamic acid, lysine, aspartic acid, valine, glycine, histidine and ornithine in order. Based on correlation coefficients of amino acid composition in the bulb, twelve garlic cultivars were classified into three major groups. Danyang, Euiseong, Seosan, Jeju, Mongol and Turkey cultivars belong to group-I, Namdo, China, Mexico and Nepal cultivars are in group-II, and group-III includes Philippine and Daeseo cultivars. Based on amino acid composition in callus, group-I includes Mongol, Euiseong, Danyang and Seosan cultivars, group-II includes Jeju, Daeseo, China and Namdo cultivars, group-III includes Napal, Mexico, Philippine and Turkey cultivars. Composition of amino acid contained in both callus and bulb is a clear standard to identify northern-type garlic cultivars. Especially the composition of amino acids in callus is more distinctive standard of classification between northern and southern type garlic cultivars than that in bulbs. 국내외에서 재배되는 12종의 마늘을 수집하여 인편과 캘러스에 함유된 아미노산의 함량을 측정하여 재배종간의 상관관계를 분석하였다. 아르기닌과 아스파라긴은 인편과 캘러스에 함유된 총 아미노산 함량의 78%이상을 차지하였으며, 그 다음으로 글루탐산, 라이신, 아스파르트산, 발린, 글리신, 히스티딘 및 오르니틴 순으로 많았다. 12개의 재배종 마늘의 인편의 아미노산 구성으로 볼 때 마늘 재배종은 3개의 주요 그룹으로 분류되었다. 첫 번째 그룹에는 단양종, 의성종, 서산종, 제주종, 몽골종 및 터키종이 포함되어 있으며, 두 번째 그룹에는 남도종, 중국종, 맥시코종, 네팔종이 포함되어 있고, 세 번째 그룹에는 필리핀종과 대서종이 포함되어 있다. 캘러스의 아미노산 구성으로 볼 때, 첫 번째 그룹에는 몽골종, 의성종, 단양종 및 서산종이 포함되어 있으며, 두 번째 그룹에는 제주종, 대서종, 중국종 및 남도종이 포함되어 있고, 세 번째 그룹에는 네팔종, 맥시코종, 필리핀 및 터키종 종이 포함되어 있다. 이러한 결과는 마늘의 인편과 캘러스에 함유된 아미노산의 조성은 한지형 재배마늘을 구분하는데 뚜렷한 기준을 제시해 주었다. 특히 캘러스에 함유된 아미노산함량 조성은 인편에 함량조성보다 한지형과 난지형을 구분하는데 더 명확한 기준을 제시해 주었다.
권순태,박상홍,주문갑,Kwon, Soon T.,Park, Sang H.,Joo, Moon G. 대한임베디드공학회 2012 대한임베디드공학회논문지 Vol.7 No.1
We propose a method for an indoor mobile robot to track user with infrared transmitter. Several infrared receivers attached around by the mobile robot enable the robot to determine the moving direction by comparing the received signal patterns. The cost of the proposed system is not only cheaper than ultrasonic system, image signal processing, RFID, and RSSI method, but also robust against environment change because any complex algorithm is not necessary. In the mobile robot, ultrasonic sensors are equipped to avoid obstacles located in the moving direction, and a simple algorithm is embedded to avoid the case of poor signal reception.
고추(Capsicum annuum L.) 배양세포의 Elicitor 유도성 Phytoalexin 생성
권순태,오세명 한국생명과학회 1999 생명과학회지 Vol.9 No.4
Extracellular capsidiol, sesquiterpenoid phytoalexin, in the medium of pepper (Capsicum annuum L.) suspension cells was not identified from control cells, but highly accumulated in the elicitor-induced cells within 6 hours after the addition of 0.05$\mu\textrm{g}$/$m\ell$ cellulase. Capsidiol production in elicitor-induced cells was markedly suppressed by cytochrome P450 inhibitors, such as ancymidol and ketoconazole demonstrating that biosynthesis of capsidiol is catalyzed by at least on hydroxylation enzyme in the biochemical pathway. Based on protein electrophoresis, two bands, 23.0kDa and 27.5kDa, were identified as newly synthesized polypeptides in the elicitor-induced suspension cells, suggesting that pepper cells which were subjected to elicitor treatment activate specific gene(s) for capsidiol biosynthesis in cultured cells.
권순태,조강희,Gwon, Sun-Tae,Jo, Gang-Hui 대한영상의학회 2003 대한영상의학회지 Vol.49 No.2
급성 횡문근융해는 골격근손상으로 인해 세포내 물질이 세포외와 혈장으로 유리되는 급성질환으로,근세포막이 파괴되는 여러 가지 근육 질환,즉 약물 또는 알코올 남용,감염,좌상,교원질병,갑작스럽고 심한 운동,지속적인 혼수상태 등으로 유발된다.최근 저자들은 일산화탄소 중독과 알코올 과다 섭취 후에 생긴 급성 횡문근융해 환자를 경험하였기에 초음파 및 자기공명영상 소견을 문헌 고찰과 함께 보고하는 바이다. Rhabdomyolysis is an acute disorder resulting from skeletal muscle injury in which intracellular contents are released into extracellular space and plasma. The condition may result from drug or alcohol overdose, infection, crush injuries, collagen disease, or intensive exercise. We report two cases of acute rhabdomyolysis resulting from CO poisoning and alcohol overdose, and discuss the MRI and ultrasonographic findings
벼 (Oryza sativa L.)배양세포의 고중력유도성 cDNA의 탐색
권순태,김길웅,Kiyoharu OONO 한국식물생명공학회 1994 식물생명공학회지 Vol.21 No.2
벼(Oryza sativa L. cv Nipponbaie)배양세포에 중력 450,000 x g를 처리하여 cDNA library를 만들고, 무처리 및 고중력을 처리한 cDNA 프로브로 스크리닝을 실시하여 고중력에 특이적으로 양성반응을 나타내는 GSC 13 및 GSC124 cDNA를 선발하였다. 선발된 두 유전자 GSC 13 및 GSC 124의 길이는 각각 1.34 및 0.67 kilobase pairs였으며, 배양세포내에서 관련된 transcript의 크기는 각각 2.0 및 1.9 kilobase pairs인 것으로 나타났다. 두 유전자를 프로브로한 Northern hybridization을 실시한 결과 GSC 13, GSC 124 공히 배양세포내에 고중력처리에 의해 특이적으로 축적되는 mRNA가 나타났으며, 중력강도 300,000 x g 에 비해 450,000 x g 처리에서 더욱 강한 축적을 보였고 450,000 x g 4시간 처리에서 최대의 수준을 보였다. Two different gravity specific cDNA, namely, GSC 13 and GSC 124 with length of 1.34 and 0.67 kilobase pairs, and transcripts of 2.0 and 1.9 kilobase pairs, respectively. were isolated by differential screening and northern hybridization of the total RNA isolated from treated and untreated cultured cells showed that maximum levels of trannscripts were achieved after 4 h of gravity stress at 450, 000 x g for both, GSC 13 and GSC 124, suggesting that these mRNA could be expressed and translated into polyeptites related to the cell to extream gravity stress.
하악골 골절 치료후 단순 방사선촬영을 이용한 추적조사의 의의
정지원,정창훈,권순태 大韓成形外科學會 1998 Archives of Plastic Surgery Vol.25 No.5
For many years, healing membranous bone fractures have been known to show a persistent lucency at the fracture interface. Radiographic follow-up has proven to be of little value as a guideline for healing of mandibular fractures. Recently, a fracture has been treated by rigid internal fixation by metallic plate and screws rather than closed reduction and wire fixation, we expected that some difference in the radiographic features of fracture healing. To investigate these questions we undertook a retrospective radiographic and clinical analysis of 33 followed patients with fractures of the body and angle of the mandible from 1993 to 1997. There were 26 male and 7 female patients ranging in age between 5 and 74 years, with an average of 29.7 years. All patients were managed by open reduction with metallic plates and screws. The length of follow-up ranged from 9 to 187 weeks. Total 81 radiographic follow-up films were obtained and divided into 3 grades according to the degree of radiolucency of fracture lines; grade 0 radiolucent fracture line and no evidence of fracture interface calcification, grade 1 decreased radiolucent area and evidence of fracture interface calcification, and grade 2 disappearance of fracture line. Until follow-up of 8 weeks, all of the radiographs showed grade 0. From 8 weeks to 16 weeks, 62.5 percent showed grade 0 and 37.5 percent showed grade 1. From 16 weeks to 48 weeks, 38.5 percent showed grade 1 and 61.5 percent showed grade 2. After follow-up of 48 weeks, all showed grade 2. In this study we have shown that the radiographic disappearance of mandibular fracture lines was usually accomplished by 48 weeks. We feel that radiographic union of the mandible is lagging well behind clinical union, but disapperance of the fracture line in rigidly fixated mandibular fracture was occurred earlier than healed by fibrous union. We propose that radiographic union of the mandible by approximately 1 year can be a guideline for the normal mandibular bone healing.