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서양계 Cymbidium의 경정배양에서 protocorm을 분화시키고 protocorm의 계대배양에서 shoot와 root의 기관 분화와 새로운 protocorm의 형성에 미치는 생장조절제, 영양인자와 비영양인자의 영향에 대하여 실험한 결과를 요약하면 다음과 같다. 1. Cymdidium의 경정조직배양에서 protocorm의 형성까지는 MS기본배지에서 8∼10주가 소용되었다. 2. MS의 기본배지에서 NAA 0.5 ppm과 BA 1.0 ppm를 혼합첨가한 것이 protocorm의 형성에 가장 효과적이다. 3. protocorm의 계대배양에서 기관분화는 NAA 0.5ppm과 BA 1.0ppm을 첨가한 배지에서 양호하였다. 4. glucose 2%와 sucrose 3%를 첨가한 MS기본배지에서 기관분화가 양호하였다. 5. NO₃-N태 질소원이 부정아와 부정근 분화와 생장에 유리하였다. 6. 활성탕의 첨가량은 1%수준이었고 경정 pH는 5.8이었다. 7. 부정아 형성에서 24시간 명배양이 유리하였고 부정근과 protocorm형성에는 12시간의 조명이 효과적이었다. Shoot tip culture of orchids is mainly practised to rapidly propagate homogeneous clones rather than the production of virus-free stocks. In the shoot tip culture of Cymbidium, the protocorms obtained was used to investigate the effects of growth regulators, nutritive and non-nutritive factors on the organogenesis. The results obtained are as follows. 1. In the shoot tip culture, the formation of protocorm required 8∼10 weeks and MS medium supplemented with NAA 0.5 ppm, BA 1.0 ppm was most effective. 2. The concentration of 0.5 ppm NAA and 1.0 ppm BA resulted in good organogenenesis from protocorm. 3. Out of sugars tested, glucose 2% showed the best result in terms of organogenesis . In addition, the sucrose concentration of 3% appeared to be optimum. 4. There was no effect of different nitrogen sources on the protocorm formation. However, the formation of adventitious shoots and roots was facilitated by the addition of NH₄NO₃or NaNO₃at 5 mM, respectively. 5. Activated charcoal was effective in the formation of adventitious shoot with the concentration at 1%. 6. Optimum pH of medium for organogenesis was 5.8. 7. Photoperiod effects on the differentiation of organs. Results showed that 24 hours for adventitious shoot and 12 hours for protocorm was effective.
, The experiment was conducted to know the reasonability of tillage effects by the treatments of different depths(0, 5, 13, and 27㎝) under the conditions of various cropping (soybean, peanut, corn, red-pepper and carrot), at the college farm of COA/CNU, Kwangju. The year of 1980 was characterized by the excessive precipitation, low light intensity and low temperature during the growing periods. The study was the preliminary summarized in scope of three volumetric changes of soils(solid-, liquid-, and gaseous phases) as affected by cultural practices mentioned above. The results obtained were as follows; 1. Volummtric ratio of solid phase was readily increased as crop advanced. This seemed to be caused mainly by excessive precipitation during the year. 2. Significant increment of liquid portion in the plots of deep tillage was detected only at early growth in the most of crops. 3. Gaseous portion which is a most critical factor on crop root elongation and proliferation was signifcantly varied by tillage depths until the late season of crop growth. 4. For the root crops(ex. carrot), possibly deep tillage is rather recommended to losening and granulation of soils than minimum or zero tillage. 5. For the cereal crops (ex. corn), leguminous(ex. soybean) and red-pepper croppings, possibly minimum or zero tillage are recommended to rationalize farm economy. 6. More detailed studies on soil physical components should be followed to know the exact and directed reasons of tillage operation on the various cropping, respectively.
Although it is suggested that risk-based management plan is needed to manage air pollution effectively, we have no resources enough to evaluate all aspects of substances and set priorities. So we need to develop a logical and easy risk-based priority setting method. However, it is impossible that only on generic system that is consistent with all the use is developed. In this study, we proposed a human health risk based priority-setting method for hazardous air pollutants, and ranked priorities for this method. First of all, after investigating previous chemical ranking and scoring systems, we chose appropriate indicators and logics to goal of this study and made a chemical priority ranking method using these. As results, final scores in priority ranking method were derived for 25 substances, and ethylene oxide, acrylonitrile and vinyl chloride were included in high ranks. In addition, same substances were highly ranked when using default values like when using no default, but the scores of hydrofluoric acid and cyan and compounds were sensitive to default values. This study could be important that priorities were set including toxicity type and quality and local inherent exposure conditions and we can set area-specific management guidelines and survey plans as a screening tool.
Background: Sporotrichosis is a chronic, deep fungal infection caused by a dimorphic fungus,Sporothrix schenckii. It is widely variable in incidence, distribution and clinical pattern. Clinical manifestations are greatly variable, with important involvement of the skin and the superficial lymphatic system. Although sporotrichosis is the most common deep fungal infection in Korea, few reports have included a long term investigation and survey of sporotrichosis. Objective: We investigated patients' records for 42 years to identify the prevalence, epidemiological features and changes in clinical findings on sporotrichosis. Methods: We carried out a retrospective analysis of 118 patients with sporotrichosis for 42 years (1997~2009) by reviewing the medical records and making phone calls to patients if needed. Results: The incidence of sporotrichosis is on a decreasing tendency at this time and incidents were more common for rural residents (80 cases, 67.8%) than urban residents (38 cases, 32.2%). Lymphocutaneous type (86 cases, 73%) is more common than fixed cutaneous type (32 cases, 27%) and the most frequent occupation of patients was farmer (71 cases, 60%). Seasonally, 42 cases (36%) occurred in winter (December to February), showing higher incidency than any other season. The most common site of the skin lesion was right upper extremity. Conclusion: We found considerable changes in prevalence, seasonal distribution and clinical findings of sporotrichosis over 42 years.
This experiment was intended to know the effects of physical and chemical properties of the excellent composts. Twenty two samples were collected from plastic covered greenhouse zone in chonnam province. The results obtained are as follows; 1. One of the most important factors in relation to the texture and other properties of the composts was the organic matter content. The higher the organic matter content were the lower the specific gravity and the apparent specific gravity and the higher the porosity. moisture content. total nitrogen and exchangeable capacity. 2. The composts for raising leaf vegetable seedlings contained higher levels of organic matter while the ones for fruit vegetable contained rather less. The organic matter contents in the composts for spring heading chinese cabbage were not variable ranging from 8.85 to 9.19 percent. 3. Hydrogen-ion concentrations of the composts were in the range of pH 4.9~7.2 but most of them were in pH 7:t1 or nearly neutral in their reaction. 4. Seven samples contained more than 1. 0m.e. of exchangeable potassium, and fifteen of them contained lower than 1.0m.e. of it. 5. It was not clear how high of P or K levels were suffieient for the excellents in some of the samples, at least, were so high, that they seemed to contain surplus amounts of fertilizers.