白鼠의 腦內 Opiate 受容體의 日中 變動에 미치는 Phenobarbital의 影響

박영걸,김기원,조규박,정성후,이화옥 전북대학교 의과학연구소 1987 全北醫大論文集 Vol.11 No.1

실험적으로 명암을 조절한 백서 뇌내 specific opiate binding β-endorphin 함량을 시간별로 정량하여 그 일중변동 유무및 이에 대한 지속적인 암적응및 phenobarbital 장기처리에 의한 영향과 opiate receptor binding과 β-endorphin 함량 양자간의 관계를 관찰하여 다음과 같은 성적을 얻었다. 1. L : D, 12 : 12 주기에 적응시킨 대조군에서 maximum[3H]-morphine binding과 뇌내 β-endorphin 함량은 22시및 06시에 최고에 달하는 매우 유의한 일중변동을 보였고 24시간 평균[3H]-morphine binding 및 뇌내 β-endorphin 함량은 각각 0.45±0.03 pmole/㎎ protein과 46.7±3.6 fmole/mg protein이었다. 2. D : D, 12 : 12 주기에 적응시킨 표본에서 maxi-mum [3H]-morphine binding과 뇌내 β-endorphin 함량은 대조군에서와 다른 02시및 14시에 최고에 달하는 시간별로 유의한 일중변동을 보였고 24시간 평균 maximum [3H]-morphine binding치는 0.36±0.03 pmole/mg protein으로 대조군에 비하여 유의한 감소를 보였고, 24시간 평균 뇌내 β-endorphin치는 35.9±3.1fmole/mg protein으로 대조군에 비하여 유의한 감소를 보였다. 3. Phenobarbital 처리 표본에서 maximum [3H]-morphine binding과 뇌내 β-endorphin 함량은 02시 및 14시에 최고, 그리고 14시 및 02시에 최저에 달하는 대조군과는 다른 일중변동을 보였으며 24시간 평균 maximum [3H]-morphine binding 치는 0.33±0.03 pmole/mg protein으로 대조군에 비하여 유의한 감소를 보였고, 24시간 뇌내 β-endorphin 함량은 60.0±5.8 fmole/mg protein으로 유의한 증가를 보였다. 4. 전실험군에서 opiate receptor binding의 Kd치는 변동되지 않았다. 5. 전 실험군에서 maximum[3H]-morphine binding은 뇌내 β-endorphin 함량과 유의한 역상관 관계를 나타내었다. 이상의 실험성적은 phenobarbital이 뇌내 β-endorphin 함량과 opiate 수용체의 숫적 변동을 일으켜 morphine의 약리적 작용을 변동시킬 수 있으며, 이와 더불어 opiate 수용체와 β-endorphin 함량 을중변동을 변화시킬 수 있음을 보여준다. To investigate the influence of penobarbital sodium on the action of morphine and the diurnal rhythms of both opiate receptor binding and β-endorphin contents, the amount of specifically bound [3H]-morphine and immunoreactive β-endorphin were measured in the midbrain of phenobarbital-treated rats at 4-hr intervals in a day. Rats were housed and adapted to a controlled cycle of either 12-hr light -12-hr dark or 24-hr constant dark. After 3 weeks of adaptation, 0.5㎖ of physiological saline or phenobarbital sodium(20㎎/㎏/day) were administered intraperitoneally twice a day for 2 weeks. Highly significant diurnal rhythms of opiate receptor binding and β-endorphin were present in rat midbrain. In the control group, the peack of maximum [3H]-morphine binding was observed at 22:00 O'clock, whereas the peak of β-endorphin contents was found at 06:00 O'clock. Even in the absence of time cues these diurnal rhythms persisted, but they were highly modified with respect to the wave forms as well differences in the timing of peak and nadir. In the phenobarbital-th\reated group, these diurnal rhythms were also modified in shape, phase and ampltude, as well as in timing of peak and nadir. In the phenobaribital-treated group, these diurnal rhythms were also modified in shape, phase and amplitude, as well as in timing of peak and nadir. In this group, 24-hr mean of opiate receptor bindindg was significantly decreased, while the 25-hr mean level of β-endorphin content was highly increased. However, Kd values in all experimental groups was not changed. This indicates that dfferences in binding were not due to changes in the affinity but in the number of binding sites. Statistical analysis of of regression line indicates that changes in receptor binding are closely correlated with the changes of β-endorphin content. These results suggest that phenobarbital may influence the action of morphin by changing the number of opiate receptors and that ine modification of diurnal rhythm of opiate receptor by the agent is possibly due to changes in β-endorphin content.

Target robot for active safety evaluation of ADAS vehicles

박영걸,이서항,박명연,신재곤,정재일 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.9

Active safety features and functions are built into a vehicle to automatically detect collision risks and prevent the occurrence of collisions. These functions are difficult to validate by using the traditional safety assessment methods. Therefore, evaluation methods and systems are developed for testing active safety systems. We developed a system for evaluating the active safety of advanced driver assistance system vehicles. This system consisted of an unmanned robot with a height of 100 mm and cloaked under a target dummy vehicle, which was made of balloons. This setup enabled the robot to pass through the underside of the test vehicle. In this manner, the evaluation system and test vehicle avoided possible damage during the collision test. The evaluation system could generate vehicular paths by using a differential global positioning system to enact a collision scenario for the assessment of the active safety function. Actual vehicle experiments were conducted to verify the system via the collision scenarios established by the Euro new car assessment program.

각막 및 수정체 질환

박영걸 대한가정의학회 1985 Korean Journal of Family Medicine Vol.6 No.1

안과 입원환자의 통계적 고찰

박영걸,이연,조성현 대한안과학회 1979 대한안과학회지 Vol.20 No.4

^(90)Sr 照射로 治療된 再發性結膜乳嘴腫

朴泳杰 全南大醫大 1980 전남의대학술지 Vol.17 No.1

Influence of Phenobarbital on the Circadian Rhythm of Opiate Receptor in Rat Brain

박영걸,김기원,조규박,Park, Yeoung-Gul,Kim, Kee-Won,Cho, Kyu-Park The Korean Society of Pharmacology 1985 대한약리학잡지 Vol.21 No.2

실험적으로 명암에 적응시킨 백서의 뇌내 specific opiate binding과 ${\beta}-endorphin$ 함량 일중변동에 미치는 지속적인 암적응, phenobarbital 장기처리의 영향을 관찰하고 opiate recptor binding과 ${\beta}-endorphin$ 함량 양자간의 관계를 추구하여 다음과 같은 성적을 얻었다. 1. L : D, 12 : 12 주기에 적응시킨 대조군에서 maximum $(^3H)$-morphine binding과 뇌내 ${\beta}-endorphin$ 함량은 각각 22시 및 06시에 최고에 달하는 매우 특이한 일중변동을 보였고 24시간 평균 $(^3H)$-morphine binding 및 뇌내 ${\beta}-endorphin$ 함량은 각각 0.45+0.03 pmol/mg protein과 46.7+3.6 fmol/mg protein이었다. 2. D : D, 12 : 12 주기에 적응시킨 표본에서 maximum $(^3H)$-morphine binding과 뇌내 ${\beta}-endorphin$ 함량은 대조군에서와는 달리 02시 및 14시에 최고에 달하는 유의한 일증변동을 보였고, 24시간 평균 maximum $(^3H)$-morphine binding치는 0.36+0.03 pmol/mg protein으로 대조군에 비하여 유의하게 감소를 하였으며, 24시간 정균 뇌내 ${\beta}-endorphin$치는 35.9+3.1 fmol/mg protein으로 대조군에 비하여 유의한 감소를 보였다. 3. Phenobarbital처리 표본에서 maximum $(^3H)$-morphine binding과 뇌내 ${\beta}-endorphin$ 함량은 각각 02시 및 14시에 최고 그리고 14시 및 02시에 최저에 달하는 대조군과는 다른 일중변동을 보였고, 24시간 평균 maximum $(^3H)$-morphine binding치는 0.33+0.03 pmol/mg protein으로 대조군에 비하여 현저한 감소를 보였다. 4. 전 실험군에서 opiate receptor binding의 Kd치는 변동하지 않았다. 5. 전 실험군에서 maximum $(^3H)$-morphine binding은 ${\beta}-endorphin$ 함량과 유의한 역상관관계가 있었다. 이상의 실험성적은 phenobarbital이 뇌내 ${\beta}-endorphin$ 함량과 opiate수용체의 숫적변동을 일으켜 morphine의 약리적 작용을 변동시킬 수 있으며, 이와 더불어 opiate 수용체와 ${\beta}-endorphin$ 함량 일중변동을 변화시킬 수 있음을 보여준다. To investigate the influence of phenobarbital sodium on the action of morphine and on the diurnal rhythms of both opiate receptor binding and ${\beta}-endorphin$ contents, the amount of specifically bound $(^3H)$-morphine and immunoreactive ${\beta}-endorphin$ were measured in the midbrain of phenobarbital-treated rats at 4h intervals in a day. Rats were housed and adapted to a controlled cycle of either 12 h light-12 h dark or 24 h constant dark. After 3 weeks of adaptation, 0.5 ml of physiological saline or phenobarbital sodium (20mg/kg/day, i.p.) were administered twice a day for 2 weeks. Highly significant diurnal rhythms of opiate receptor binding and ${\beta}-endorphin$ were present in rat midbrain. In control group, the peak of maximum $(^3H)$-morphine binding was observed at 22:00 h, whereas the peak of ${\beta}-endorphin$ content was found at 06:00 h. Even in the absence of time cues these diurnal rhythms persisted, but they were highly modified with respect to the wave form as well as differences in the timing of peak and nadir. In the phenobarbital-treated group, these diurnal rhythms were also modified in shape, phase and amplitude, as well as in timing of peak and nadir. In this group, 24 h mean of opiate receptor binding was significantly decreased, while the 24 h mean level of ${\beta}-endorphin$ content was highly increased. However, Kd values in all experimental groups did not change. This indicates that differences in binding were not due to changes in the affinity, but in the number of binding sites. Statistical analysis of regression line indicates that changes of receptor binding were closely correlated with the changes of ${\beta}-endorphin$ content. These results suggest that phenobarbital may influence the action of morphine by changing the number of opiate receptors and that the modification of diurnal rhythm of opiate receptor by the agent is possibly due to changes of ${\beta}-endorphin$ content.

眼斜筋의 張力에 미치는 半規管의 相互作用 樣態

朴泳杰 全南大醫大 1977 전남의대학술지 Vol.14 No.2

심포지움 : 하드 콘택트렌즈에 의한 시력교정

박영걸 대한안과학회 1983 대한안과학회지 Vol.24 No.2

LM guide 의 등가 강성 및 열적 모델 개발

박영걸(Yeonggeol Park),정재일(Jayil Jeong) (사)한국CDE학회 2013 한국 CAD/CAM 학회 학술발표회 논문집 Vol.2013 No.8

Hydroxyapatite 안와삽입술의 임상보고

박영걸(Yeoung Geol Park),정종학(Jong Hak Jeong),정상기(Sang Ki Jeong) 대한안과학회 1996 대한안과학회지 Vol.37 No.11