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Dot Enzyme Immunoassay법과 RT - PCR을 사용하여 국내에서 분리되는 일본뇌염 바이러스의 조기 진단법 개발
조해월,김정림,반상자,남재환,정연준,김은정,이유진,최승은,원은하 대한바이러스학회 1995 Journal of Bacteriology and Virology Vol.25 No.2
To the development of the rapid viral diagnosis for Japanes Encephalitis (JE), we established the dot enzyme immunoassay (DEIA) and RT-PCR to detect an antigen and antibody against JE virus. For detection of JE antibody in swine serum using the DEIA method, we confirmed 100% of the sensitivity and the specificity comparison to the ELISA and HI method. Beside, using three method: SDS-Proteinase K-phenol method, RNA Tack resin Kit (Biotecx) and Guanidine thiocyanate Kit (Promega) for isolation RNA of JE virus. Among these method, RNA Tack resin Kit was detected to JE virus even 10'dilution level which HA titer was 1:320 and this method is the most rapid isolation of RNA. The sensitivity of RT-PCR was found to be at least 10 to 10better, as compared to that of HA test. With the results, DEIA and RT-PCR is rapid di- agnosis method to detection for JE virus and it could be applied to the other viral disease for rapid viral diagnosis.