한국에 분포되어 있는 토착대두 근류균의 질산 환원 특성

최영주,이상규,조무제,최용락,윤한대,유진창 한국콩연구회 1987 韓國콩硏究會誌 Vol.4 No.1

연속적으로 대두를 재배한 토양과 신개간지 토양에서 재배한 대두 품종 단엽의 근류로부터 토착대두 근류균 87종을 분리하고 이들을 생장속도에 따라 Bradyrhizobium japonicum((slow-grower) 및 Rhizobium fredii(fast-grower)로 분류한 결과 전자가 55종, 후자가 32 종이었다. 이두그룹의 대두 근류균을 질산염 환원특성에 따라 denitrifying fastgrower(F-1), nitrate respiring fast-grower (F-Ⅱ), denitrifying slow-grower(S-I), 및 nitrate respiring slow-grower(S-Ⅱ)로 세분 되었으며, 분리균주 총 87종중 S-Ⅰ, S-Ⅱ, F-I 및 F-Ⅱ의 수는 각각 10, 22, 48및 7종이었다. 이들 각 group 대두 근류균의 균체단백질의 전기영동 pattern상의 차이를 1차 및 2차원 전기영동으로 분석한 결과 slow및 fast-grower간에는 현저한 차이를 관찰할 수 있었으나 동일생장속도를 가진 것 중 denitrifer와 nitrate respirer간에는 큰 차이가 없었다.

노안교정을 위한 굴절형 각막 인레이의 장기간 임상 결과

최영주,한겨레,임동희,정태영 대한안과학회 2023 대한안과학회지 Vol.64 No.2

Purpose: To study the long-term efficacy and safety of Flexivue Microlens refractive corneal inlays in Koreans for compensation of presbyopia. Methods: Flexivue Microlens inlays were implanted in nine eyes at Samsung Medical Center from October 2015 to February 2018. The follow-up period was 1.95 ± 0.6 years. Pre- and postoperative near/intermediate/distant visual acuity, spherical equivalent, contrast sensitivity, defocus curve, keratometry, and patient satisfaction were evaluated retrospectively. Results: The uncorrected near visual acuity (UNVA) of the operated eye increased significantly to 0.27 ± 0.10 logarithm of the minimal angle resolution (logMAR) after 6 months (p = 0.012). At the last observation, it was 0.32 ± 0.21 logMAR, which improved compared to before surgery, but the difference was not significant (p = 0.127). The binocular UNVA improved significantly to 0.19 ± 0.78 logMAR at 6 months postoperatively (p = 0.017) and to 0.21 ± 0.13 logMAR at the last observation (p = 0.028). There was no difference in the binocular uncorrected distant visual acuity (UDVA) before and after surgery, but the UDVA of the operated eye decreased significantly to 0.38 ± 0.15 logMAR at 6 months postoperatively (p = 0.007) and to 0.32 ± 0.21 logMAR at the last observation (p = 0.012). Satisfaction with near vision improved significantly after surgery; 14.3% of the patients had a score of 4 (good) or higher and 42.9% did not require near-vision glasses. In one case, the inlay was removed after 17 months due to blurred vision. Conclusions: The Flexivue Microlens has a low risk of complications and is a reversible technique. However, the near vision improvement was temporary and distance vision deteriorated. In addition, the Koreans examined had relatively low satisfaction and high spectacle dependence. 목적: 한국인에서 노안교정을 위한 Flexivue Microlens의 효능과 안전성에 대한 장기간 임상 결과를 분석하였다. 대상과 방법: 2015년 10월부터 2018년 2월까지 삼성서울병원에서 Flexivue Microlens를 삽입한 환자 9명을 대상으로 하였고, 평균추적 기간은 1.95 ± 0.6년이었다. 수술 전후 시력, 구면렌즈대응치, 대비감도, 탈초점곡선, 환자 만족도에 대해 후향적으로 의무기록을 분석하였다. 결과: 삽입안의 근거리 나안시력은 술 후 6개월에 0.27 ± 0.10 logarithm of the minimal angle resolution (logMAR)으로 향상되었고(p=0.012), 마지막 관찰 시 0.32 ± 0.21 logMAR로 술 전에 비해 개선되었으나 유의한 차이는 없었다(p=0.127). 양안 근거리 나안시력은 술 후 6개월과 마지막 관찰 시 각각 0.19 ± 0.78 logMAR, 0.21 ± 0.13 logMAR로 향상되었다(p=0.017, p=0.028). 삽입안의 원거리 나안시력은 술 후 6개월과 마지막 관찰 시 각각 0.38 ± 0.15 logMAR, 0.32 ± 0.21 logMAR로 감소하였으나(p=0.007, p=0.012), 양안에서는 수술 전후 차이가 없었다. 근거리 시력에 대한 만족도는 유의하게 향상되었으며, 4점(만족) 이상은 14.3%였고 근거리 안경을 착용하지 않는 비율은 42.9%였다. 1명은 흐려 보임으로 1년 5개월째 인레이를 제거하였다. 결론: Flexivue Microlens는 합병증 위험이 낮고 가역적이지만, 근거리 시력 개선이 단기적이고 원거리 시력이 저하되었다. 또한 한국인에서 만족도가 낮고 안경 의존도가 높았다.

Enterobacter agglomerans CBNU45로부터 분리된 제한효소 EagBI 의 특성

최영주,김성재,황혜연,임정빈,김영창,Choe, Yeong-Ju,Kim, Seong-Jae,Hwang, Hye-Yeon,Im, Jeong-Bin,Kim, Yeong-Chang 한국미생물학회 1994 미생물학회지 Vol.32 No.1

토양에서 분리된 Enterobacter agglomerans CBNU45는 type II 제한효소인 EagBI을 생산하고 있음을 발견하였다. EagBI을 DEAE-cellulose, phosphocellulose P11, hydroxylapatite column chromatography를 거쳐 부분 정제하여 그 특성을 알아보았다. EagBI은 여섯개의 염기배열 5’-CGAT${\downarrow}$CG-3’을 인식하고 T 와 C 사이를 절단하여 두개의 염기가 3’-말단쪽으로 돌출된 cohesive end를 형성하였다. EagBI의 반응 최적조건은 10mM Tris-HCl(pH 7.8), 6~10mM $MgCl_2$, 37${\circ}C$이었으며 NaCl이 없는 반응 완충용액에서 가장 좋은 활성을 보였다. EagBI은 $dam^-$와 $dam^+$ 메칠화 DNA도 절단할 수 있으며 65${\circ}C$에서 10분 동안 열처리하였을 때 효소의 활성을 상실하였다. 따라서 EagBI은 PvuI의 isoschizomer이나 NaCl 요구성과 열안정성에서 PvuI보다 편리한 제한효소로 확인되었다. EagBI is a type II restriction endonuclease from Enterobacter agglomerans strain CBNU45 isolated from soil. EagBI was partially purified by DEAE-cellulose, phosphocellulose P11 and hydroxylapatite column chromatography. EagBI recognizes and cleaves the sequence 5'-CGAT${\downarrow}$CG-3' and generates 2-base 3'-protruding cohesive ends. The optimal reaction conditions of EagBI are 10 mM Tris-HCl (pH 7.8), 6-10 mM $MgCl_2$, at 37 ${\circ}C$. The enzyme is maximally active in the absence of NaCl, able to cleave both $dam^-$ and $dam^+$ DNAs, and sensitive to heat treatment (at 65 ${\circ}C$ for 10 min). Therefore, although EagBI is an isoschizomer of PvuI, it is more useful than PvuI in respect of the NaCl requirement and heat-stability.

[발제문] 〈밀키웨이〉 : 휴머니즘의 온화함과 유보된 현실인식

최영주 공연과이론을위한모임 2008 공연과 이론 Vol.- No.32

Dissimilatory Nitrate Reduction Characteristics of Indigenous Soybean Rhizobia Distributed in Korea Soils

최영주,최용락,윤한대,유진창,이상규,조무제,Choi, Young-Ju,Choi, Yong-Lark,Yun, Han-Dae,Ryu, Jin-Chang,Lee, Sang-Kyu,Cho, Moo-Je 한국응용생명화학회 1986 Applied Biological Chemistry (Appl Biol Chem) Vol.29 No.2

연속적으로 대두를 재배한 토양과 신개간지 토양에서 재배한 대두 품종 단엽의 근류로부터 토착대두 근류근 87종을 분리하고 이들을 생장속도에 따라 Bradyrhizobium japonicum (slow-grower) 및 Rhizobium fredii (fast-grower)로 분류한 결과 전자가 55종, 후자가 32종이었다. 이두그룹의 대두 근류균을 질산염 환원특성에 따라 denitrifying fast-grower(F-1), nitrate respiring fast-grower(F-II), denitrifying slow-grower(S-I), 및 nitrate respiring slow-grower (S-II)로 세분 되었으며, 분리균주 총 87종중 S-I, S-II, F-I 및 F-II의 수는 각각 10, 22, 48 및 7종이었다. 이들 각 group 대두 근류균의 균체단백질의 전기영동 pattern상의의 차이를 1차 및 2차원 전기영동으로 분석한 결과 slow 및 fast-grower간에는 현저한 차이를 관찰할 수 있었으나 동일 생장속도를 가진 것 중 denitrifer와 nitrate repirer간에는 큰 차이가 없었다. Eightyseven strains of indigenous Rhizobia were isolated from the nodule of soybean cultivar, Danyup, cultivated in four different soils sampled from continuously soybean cultivated and newly reclaimed fields. The strains were grouped into Bradyrhizobium japanicum (slow-grower:55 strains) and Rhizobium fredii (fast-grower: 32 strains). The both groups could be divided into two sub-groups according to the denitrification characteristics, that is, denitrifying fast-grower (F-I), nitrate respiring fast-grower (F-II), denitrifying slow-grower (S-I). and nitrate respiring slow-grower (S-II). Among the 87 isolates, F-I, F-II, S-I and S-II sub-groups were 10, 22, 48 and 7 strains, respectively. The one-and two-dimensional polyacrylamide gel electrophoretic pattern of the four sub-groups were compared and discernible difference was observed between fast and slow-grower, but the difference was not discernible between subgroups within the same growth rate group.

한국의 페미니즘 연극, 그 현황과 과제

최영주 국제언어인문학회 2004 인문언어 Vol.6 No.-

Considering Korean feminist theatre is not successful at all these days, it is needed to find the reasons of its crisis. As two Korean feminist theatre scholars argued, the crisis owed to the structural problem from the start. That is the Korean feminist theatre started and developed depending on the imported popular feminist plays without social and cultural self-consciousness. Once the imported feminist theatres were flourished, some theatre companies pursued the commercial success blurring the feminist issues. It was resulted into the intentional ignorance in and out of the theatre society. While, some feminist plays were too inclined to the agit-prop without artistic sophistication. This essay tries to examine how the feminist theatres have been developing and what kinds of feminist performances have been made until now. And it intends to emphasize that the play text should be based on the Korean women's past, present, and future reality. Besides, they have to delve into the problem by which the Korean women were trapped in historical, social, and cultural environment. To make the women's matter the social issue at present and for the future, the Korean feminist theatre should re-find its place as the socio-cultural forum. First, Korean theatre should cooperate with the other women's group crossing the different disciplines of the society, the culture, the politics etc. Secondly, we need to observe and watch where and how the distortion happens in women's matter, and react to correct it. Thirdly, we need to discover, to support, and to protect the women centered perspective of some playwrights as well as the performers. Co-writing or co-performing is also very positive to diversify the women's subjects. Lastly, to protect the feminist theatre against the consumerism, they need to have the financial support from the government or some civil society.

미디어가 주선한 실제와 환상의 접촉 : <887>

최영주 한국연극평론가협회 2019 연극평론 Vol.94 No.-

허구와 실재 그 경계의 확장

최영주 한국연극평론가협회 2018 연극평론 Vol.88 No.-

Tissue Specific Expression of Wound-Inducible RCaM-2 Promoter in Transgenic Tobacco Plants

최영주,Choi Young Ju Korean Society of Life Science 2005 생명과학회지 Vol.15 No.2

To study calmodulin (CaM) gene expression and its regulation, rice CaM promoter (RCaM-2) was isolated and fused to $\beta-glucuronidase$ (GUS), reporter gene. X-Glue staining patterns revealed that GUS localization is high in meristemic tissues such as the stem apex, stolen tip, and vascular regions. GUS staining in the transverse sections of stem and petiole was restricted to the inside of the vascular system, and cortex and epidermis located outside of the vascular system usually did not show GUS staining even a plant that expressed strong activity. GUS activity was found to be tissue specific expressed and exhibited a dramatic transient increase in response to wounding. These results suggest that the 5'-flanking region of RCaM gene regulates wound-inducible expression. Calmodulin 유전자의 발현 조절을 연구하기 위해, 벼 calmodulin promoter (RCaM-2)를 분리하여 GUS (report 유전자)에 융합하였다. GUS 활성은 정단조직, 근단 및 관다발 영역과 같은 성장조직에서 높게 발현되었다. 줄기와 페티올의 transverse 절단부위 GUS 활성은 관다발계의 안쪽에 제한되었으며 관다발계의 외부에 위치한 피층과 표피에서는 강하게 발현된 식물에서도 GUS 활성이 나타나지 않았다. GUS 활성은 어린 조직에서 특이적으로 발현되었으며 상처에 의해서 신속하게 증가하였다. RCaM-2 promoter는 세포분열이 왕성한 어린조직이나 생장점에서 강하게 발현되며 mechanical 신호에 의해서 현저히 유도되었다. 이러한 결과는 RCaM-2 유전자의 5'-flanking 영역이 상처에 의해서 유도되는 발현을 조절하는 것으로 추정된다.

Corpus Based Collocation Analysis of the Phrasal Verb Stand Up

최영주 21세기영어영문학회 2016 영어영문학21 Vol.29 No.2

When analyzed appropriately, studies of corpus data aid in our understanding of the current usage of words and phrases. For lexical phenomena, lexical partnership or collocation has been the focus of many corpus studies as it is reliably and comprehensively identified in corpora. Nonetheless, studies on collocational patterns of phrasal verbs are rare. This paper showcases the collocational partners of the phrasal verb stand up as observed in the COCA corpus. The paper shows the following results: first, new meanings are harvested through the collocational analysis, when the meanings observed in COCA are compared with those defined in the Longman Dictionary of Contemporary English; second, among various collocates, the prepositions to, for, and against and the adverb well/better are strongly associated with metaphorical meanings; third, the base form, stand up, is more closely connected to metaphorical meanings of STAND UP than other forms such as stood up, standing up, and stands up; fourth, among various other metaphorical meanings, ‘to face boldly' is the most frequent.