Characteristic Fluorescence Response of (6-Hydroxy-2-naphthyl)ethenyl Pyridinium Dyes with Bovine Serum Albumin

최명석,Ankush Gupta,서정화,S. Velmathi,James N. Wilson,Jong S. Park 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.1

In this study, we report the efficient identification of proteins using (6-hydroxy-2-naphthyl)ethenyl pyridinium dyes (HNEPs). These dyes exhibited characteristic fluorescence responses, showing enhanced fluorescence with a red shift in the case of bovine serum albumin. The nature of the interaction between the dye and the protein was examined using a combination of various spectroscopic techniques. A time-dependent density functional theory analysis supported the strong environment-dependent fluorescence emission properties of the dyes. The current investigation clearly demonstrates that a selective optical response HNEPs can find potential usefulness as bio-sensing probes.

한국자생 음나무집단 및 채취부위에 따른 Kalosaponin 함량 변이

최명석,권기원,이철호,Choi, Myung-Suk,Kwon, Ki-Won,Lee, Cheol-Ho 한국생약학회 2000 생약학회지 Vol.31 No.2

The concentrations of 4 kalosaponins from tissues of Kalopanax septemlobus (Thunb.)Koidz grown in 7 provenances in Korea were determined by HPLC. Kalosaponin contents in plant part were much higher in the inner bark(30.59 mg/g on the dry weight basis) than those of young leaves(22.74 mg/g on the dry weight basis) and root bark(18.02 mg/g on the dry weight basis). A considerable range of variation in the contents was observed among population. The kalosaponin contents in inner bark from each population were highest in the Mt. Barwang (30.37 mg/g on the dry weight basis) followed by Mt. Gariwang, Hanra II, Mangun, Paltan, and Hanra I population. A variation of kalosaponin contents among population may be affected by both environmental and genetic factors. Establishment of selection and propagation of high kalosaponin containing trees can be a good source for the development of valuable forest products.

폴리우레탄 합성을 위한 새로운 사슬연장제의 합성

최명석,강인규,이영무,성용길 한국고분자학회 1995 한국고분자학회 학술대회 연구논문 초록집 Vol.1995 No.4

Production of Ginkgolides and Bilobalide from Optimized the Ginkgo biloba Cell Culture

최명석,김선원,정영관,박용구,Su-Jung Kim,Young-Min Kang,Hee-Young Jung,D. Theertha Prasad 한국생물공학회 2004 Biotechnology and Bioprocess Engineering Vol.9 No.1

The influence of various culture conditions on growth and ginkgolides (GKA and GKB), and bilobalide formation in callus and suspension cultures of Ginkgo biloba were investigated. Callus induced from the leaf petioles exhibited distinct morphological and physiological responses. The cell biomass and ginkgolides content varied among the cell lines; brownish callus lines produced high levels of ginkgolides and bilobalide in spite of poor cell growth. Among the culture media used, MS medium showed significant effect on cell growth and ginkgolides production. Low concentration of sucrose (3%) improved cell growth, while higher sucrose levels (5 and 7%) improved ginkgolides production. Cultivation of callus cultures above 28oC dramatically reduced their growth rate; however the cell lines grown at 36oC showed increased levels of bilobalide content. A 2.5-L balloon type bubble bioreactor (BTBB) was successfully developed for the cell growth and ginkgolides production.

Efficient Release of Ferulic Acid from Sweet Potato (Ipomoea batatas) Stems by Chemical Hydrolysis

최명석,이종윤,이병현,정강원,Chandrakant S. Karigar,Ji-Hyun Park,Ji Yun Min,Bu-Kug Lim,양재경 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.3

An efficient method for release of ferulic acid from sweet potato stems was developed. Ferulic acid along with phenolic compounds were released from stems by acid and alkaline treatments. The base hydrolysis with 0.1 N NaOH yielded the highest quantity of total extracts (471.1 mg/g). The stems released more phenolic compounds when 0.0125~0.025 N NaOH was employed. Where as ferulic acid release was maximal with 0.05 N H₂SO₄ (0.32 mg/g). Ferulic acid was separated from phenolics by column chromatography. Among the elution solvents, ethyl acetate fractions (80%) contained ferulic acid. Ethyl acetate eluants were further fractionated with n-hexane/ethyl acetate/formic acid (100/50/0.5, v/v/v). All fractions showed ferulic acid and phenolic compounds. Fraction V among them was ascribed to ferulic acid with an yield of 5.41 mg/g of dry sweet potato tissue.

유망 산채의 산업적 잠재성

최명석,양재경,Choi, Myung-Suk,Yang, Jae-Kyung 한국식품연구원 2008 食品技術 Vol.21 No.1

폐에 발생한 원발성 융모막 상피종 - 1예 보고 -

최명석 대한흉부심장혈관외과학회 1990 Journal of Chest Surgery (J Chest Surg) Vol.23 No.5

Primary choriocarcinoma of the lung is extremely rare and have been reported ten and several cases only in the literature. It is very difficult to confirm the "Pure primary pulmonary choriocarcinoma" clinically, so most cases that have been reported in the literature confirmed by autopsy and the prognosis is relatively poor compared with metastases. We experience one case of the primary pulmonary choriocarcinoma. She was 53 year old multiparous woman[4 \ulcorner0 \ulcorner2 \ulcorner0] who complain right chest pain for 4 years. On plain film and computerized tomogram of the thorax at admission, 2.5cmx3.5cmx5 cm sized, well demarcated ovoid mass was founded on the right middle lobe just below the parietal pleura and growing rapidly on plain film to 5cm x 6.5cm x 7cm after 15 days. We confirm the choriocarcinoma in the lung by percutaneous needle aspiration biopsy and strongly suspect primary after various examination for rule out metastases from the ovary or uterus. We perform middle lobe lobectomy because that can not confirm the primary or the metastases because there are not complete histological examination by bilateral ovariectomy and hysterectomy, and under the belief that extragestational or extragonadal primary choriocarcinoma is more resistant to the chemotherapy, and could be reduce the duration of hospitalization and the amount of chemotherapy used to achieve remission On 14th postoperative day, serum p-HCG level was returned to normal limit, and perform chemotherapy two times on 3rd and 5th week for prevention surgical traumatic hematogenous metastases or undetected microfocus, but the patient expire on 68th postoperative day due to intracerebral hemorrhage in the intracranial choriocarcinoma which strongly suspected surgical traumatic hematogenous metastases.etastases.

성인에서 발견된 선천선 식도 - 기관지루 수술 치험 -1예 보고-

최명석 대한흉부심장혈관외과학회 1990 Journal of Chest Surgery (J Chest Surg) Vol.23 No.5

Congenital bronchoesophageal fistula is a rare anomaly that can appear in adult life uncommonly. There was a largest review that include 100 cases in the literature. In our case, he was 48 years old male patient who admitted for chronic coughing and recurrent lobar pneumonia on the right lower lobe since 10 years old. We could confirmed the fistular preoperatively by barium swallow examination and performed division of the fistula. The patient’s postoperative course was unremarkable. According Braimbridge’s classification, it was belonged to the type II.

폐내 과오종

최명석 대한흉부심장혈관외과학회 1990 Journal of Chest Surgery (J Chest Surg) Vol.23 No.6

Hamartoma had been considered as a congenital malformation of a tumerous lesion since the original description by Albrecht in 1904. Pulmonary hamartoma is able to classified as endobronchial and intrapulmonary hamartoma clinically, and is considered to benign mesenchymal neoplasms which originated in peribronchial connective tissue, and so called mesenchymoma by someone. We have experienced 3 cases intrapulmonary hamartoma which were discovered during routine chest film check up at all cases and are able to detailed histologic examination by electron microscopy at one case.

흉부 손상에 대한 임상적 고찰

최명석 대한흉부심장혈관외과학회 1990 Journal of Chest Surgery (J Chest Surg) Vol.23 No.5

A clinical evaluation was performed on 545 cases of the chest trauma those had been admitted and treated at the department of thoracic and cardiovascular surgery in Chosun University Hospital during the past 11 years 5 months period from January 1978 to may 1989. Obtained results were as follows: 1. The ratio of male to female was 3.9: 1 in male predominance, and the majority[66.6%] was distributed from 3rd to 5th decade. 2. Nonpenetrating chest trauma was more common than penetrating about 4.6 times, and the most common cause of the nonpenetrating injuries was traffic accident[241/448, 53.8%] and of the penetrating injuries was stab wound[88/97, 90.7%]. 3. Only 79 cases[14.5%] were arrived to our emergency room within one hour after trauma. 4. The most common lesion due to trauma among these admitted patients was rib fracture[390/545, 71.6%], and the others were lung contusion[217/545, 39.8%], hemothorax[35%], hemopneumothorax[19.6%], and pneumothorax[11.8%] et al in decreasing order. 5. The associated injuries those required special treatment of other departments were 223 cases and its distributions were bone fractures[178/545, 32.7%], head injury[5.3%], and abdominal injury[6.6%]. 6. The others, but interesting chest injuries were follows: sternum fracture[3.1%], diaphragm rupture[2.6%], myocardial laceration and rupture[2 cases], bronchial rupture and laceration[2 cases], and traumatic thymoma rupture[1 case]. 7. The incidence of flail chest was 5.8%a[26/448] in the nonpenetrating injury, and the causes were multiple rib fracture which was in rows more than 4 rib fracture[20 cases], and sternum fracture[6 cases]. 8. We could managed the most of the patient with conservative treatment[43.1%] or closed tube thoracostomy[52.7%], but required emergency open thoracotomy in 64 cases