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가시오갈피 생물반응기 배양에서 질소급원이 세포생장과 이차대사 생산에 미치는 영향
안진권,이위영,박소영,Ahn, Jin-Kwon,Lee, Wi-Young,Park, So-Young 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.3
The effects of inorganic nitrogen sources such as KNO$_3$ and NH$_4$ NO$_3$ on cell growth and production of chlorogenic acid and eleutheroside E derivative were investigated in 5L bioreactor cultures of Eleutherococcus senticosus. The cell growth in the 1/2MS medium containing 15mMKNO$_{3}$. The fresh weight of cells harvested from bioreactor was affected by the concentration ratio of NO$_3$$^{[-10]}$ and NH$_4$$^{+}$ in culture medium. At the viewpoint of secondary metabolite production, the production of chlorogenic acid was affected by the concentration of NH$_4$$^{+}$ in the culture medium, but not by the total concentration of nitrogen sources in the culture medium. Futhermore, eleutheroside E derivative production was also affected by the concentration ratio of NO$_3$$^{[-10]}$ and NH$_4$$^{+}$ in the culture medium. Base on those results, it is suggested that cell growth and production of secondary metabolite(chlorogenic acid and eleutheroside E derivative) could be manipulated by controlling the total concentration of nitrogen sources and the concentration ratio of NO$_3$$^{[-10]}$ and NH$_4$$^{+}$ in the culture medium. medium.
가시오갈피나무 부정근(不定根) 배양(培養)에서 부정근(不定根)의 생장(生長)과 Eleutheroside유(類)의 생산(生産)에 미치는 Salicylic Acid 처리(處理)의 영향
안진권 ( Jin Kwon Ahn ),이위영 ( Wi Young Lee ),박응준 ( Eung Jun Park ) 한국임학회 2011 한국산림과학회지 Vol.100 No.2
생물반응기를 이용한 가시오갈피 부정근 배양시에 salicylic acid를 농도별(0, 5, 10, 20, 40, 80 μM)로 처리하여 부정근의 생장과 eleutheroside류 생산과의 관계를 조사하였다. Salicylic acid 처리농도별 부정근의 생장은 무처리구에서 4.4 g dry weight(DW)/L로 가장 높았으며, salicylic acid 농도가 증가할수록 부정근 생장은 감소하였다. 그러나 eleutheroside B, E 및 E1의 함량은 대체로 salicylic acid 농도가 높을수록 증가하여 80 μM 처리구에서 각각 179.5, 1169.9 및 45.4 μg/g DW로 생산량이 가장 많았다. 배지 1 L당 eleutheroside류의 총생산량은 무처리구에서 4975.8 μg/L를 생산하여 가장 우수하였다. 80 μM의 salicylic acid 처리 후 15일간 eleutheroside류의 함량을 조사한 결과 eleutheroside B는 salicylic acid처리 후 9일째, eleutheroside E 및 E1은 처리 후 6일째 각각 가장 많은 생산량을 보여주었다. This study was carried out to investigate the dose-dependent effect of salicylic acid on both the adventitious root growth and the accumulation of various eleutherosides in the bioreactor culture of Eleutherococcus senticosus. The highest biomass production (5.4 g DW/L) was observed in the absence of salicylic acid, while the root growth was significantly decreased by increasing the concentration of salicylic acid. Salicylic acid stimulated the production of both eleutheroside B, E and E1. The highest levels of eleutheroside B (179.5 μg/g DW), E (1169.9 μg/g DW) and E1 (45.4 μg/g DW) were obtained by the addition of 80 μM of salicylic acid. The maximum eleutheroside production was 4975.8 μg/L when salicylic acid was not added. In addition, when the adventitious roots were cultured in the basal medium supplemented with 80 μM of salicylic acid, the highest levels of eleutheroside B was observed at the 9th day, while eleutheroside E and E1 were observed at the 6th day, respectively.
안진권,박영기,이위영,박소영 한국산림과학회 2007 한국산림과학회지 Vol.96 No.5
This study was caried out to investigate the impacts of jasmonic acid (JA) on adventitious rootculture of Eleutherococcus senticosus. Adventitious root of E. senticosus were treated with jasmonic acid(JA) and cultured for 30 days. JA inhibited the root growth but increased eleutherosides accumulation, totaltotal phenolic contents in E. senticosus adventitious root to 39.81 g/g, about 2.6 times higher than that ofthe control. Consequently, high acumulation of total phenolic contents led to increase the antioxidantactivity to 82.41%. The antioxidant activity of control was 37.89% at 250 g/mL. A linear correlation (R2= 0.9937, 0.9648 and 0.983) was also shown between antioxidant activity (at 1250, 1875, and 2500 g/E. senticosus.