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미치광이풀 모상근 배양에서 적정 sucrose 농도 및 hydroxyapatite 첨가에 의한 tropane alkaloid 생산성 향상
안준철,양선주,표병식,최지원,황백,An, Jun-Chul,Yang, Sun-Ju,Pyo, Byung-Sik,Choi, Ji-Won,Hwang, Baik 한국식물생명공학회 1998 식물생명공학회지 Vol.25 No.1
미치광이풀(Socopolia parviflora Nakai) 모상근 배양에서 모상근 생장과 tropane alkaloid 생산에 대한 sucrose농도와 일부 흡착제의 효과를 조사하였다. 모상근 클론 SP 11은 5% sucrose 농도를 함유한 1/2 B5 배지에서 최적인 생장과 tropane alkaloid 생산을 보였다. 모상근에서 tropane alkaloids 생성양상은 모식물에서와 약간의 차이를 보였고 그 중 식물체에서 검출되지 않는 littorine의 생성이 모상근에서 두드러졌다. 조사된 흡착제(0.01% 1%) 중에서, charcoal 0.01%와 XAD-II l%에서 약간의 생장촉진효과를 보인 것을 제외하고는 그 외 농도나 흡착제(amberlita chitosan)에서는 억제효과 또는 유의적인 변화를 보이지 않았다. 반면에 hydroxyapatite는 미치광이풀 모상근의 생장과 tropane alkaloid 생산에 있어 촉진효과가 있음이 확인되었다. The effects of sucrose concentration and some absorbents on growth and tropane alkaloid production in hairy root cultures of Scopolia parviflora were investigated. The maximum effect on growth and tropane alkaloid production in hairy root clone SP11 was obtained for 1/2 B5 medium containing 5% sucrose. The production pattern of tropane alkaloid in hairy roots was some different from that of rhizome of mother plant, particulary showing high littorine contents, which was not found in ordinary roots. Among absorbents examined, charcoal 0.01% and XAD-II 1% made a slight growth promotion effect, whereas the other concentration of charcoal, XAD-II and absorbents (amberlite and chitosan) showed inhibition or no significant effect. The addition of hydroxyapatite enhanced the production of tropane alkaloids significantly than control cultures.
시호 모상근 배양에서 생장과 시호 사포닌 생합성에 미치는 배지와 IAA 및 IBA의 영향
안준철,김응식,이현진,황백,Ahn, Jun Cheul,Kim, Eung Sik,Lee, Hyun Jin,Hwang, Baik 한국식물생명공학회 1999 식물생명공학회지 Vol.26 No.3
시호 모상근 (BFHR2 클론) 배양에서 생장과 saikosaponin 생산에 미치는 기본배지, 배지의 다량무기염농도 변화 및 IAA와 IBA의 효과를 조사하였다. 조사한 배양액 중 RCM 배지에 다량무기염농도를 2배로 증가시킨 배지에서 생장이 가장 양호하였다. 또한 2RCM배지에서는 saikosaponin인 a, c와 d의 생성을 확인하였으며, 반면에 MS배지에서는 saikosaponin의 생성이 극히 저조하였다. IBA는 0.5mg/L에서 약 60%의 생장촉진효과를 보였으나, IAA와 IBA두 가지 모두 0.01~5mg/L 까지의 농도에서 뿌리형태의 외적인 변화 없이 saikosaponin 생성을 강하게 억제하는 작용을 나타내었다. We examined the effects of various basal media and exogenous auxins on the growth and saikosaponin production in hairy root cultures of Bupleurum falcatum. Of the various media tested, 2RCM medium was superior for growth and saikosaponin content, in which saikosaponin a, c, and d was produced, however MS medium was resulted in extremely low level in saikosaponin production. By the treatment of 0.5 mg/L IBA the growth rate was increased by about 60% in 2RCM medium, whereas both IAA and IBA showed a potent inhibition effect in saikosaponin biosynthesis by the treatment of 0.01 mg/L~5mg/L.
저널리즘 생산자로서 '문제적 기자'에 관한 연구 - 민속지학적 방법을 중심으로 -
안준철,김영찬 한국외국어대학교 국제커뮤니케이션연구소 2019 東西言路 Vol.45 No.-
The study began with a focus on social phenomena in which producers, journalists, are in the spotlight rather than articles, which are the result of journalism. After an era of exclamations, exclusive or in-depth articles, recent audience of journalism have begun to attack journalists. The behavior, which began from a critical perspective on the results of journalism produced by a particular journalist, came at last to attach the scornful term ‘Kireki(journalist+trash)’ to journalists. Many people are concerned about this social phenomenon as a ‘crisis of journalism’. Therefore, the crisis phenomenon of journalism was approached through in-depth interview with journalists who are considered to be representative 'problematic journalist' among journalists and news consumers. The ‘problem journalists’ had something in common that the way they entered the world of journalists was not orthodox and did not have a proper journalism education. They pretended to be a fanatic even before entering the world of journalist in the field they were covering, and after becoming a journalist, they were either overly friendly to what they liked or obsessed with comments advocating their own articles. However, the fact that they have become 'problematic journalists' could not simply turn it into an individual problem. The reality that the control of the newsroom has become meaningless due to changes in the media environment, and the excessive authority given to journalists' individuals have also made them problematic journalists.