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천마(天麻)가 뇌조직출혈(腦組織出血) 흰쥐의 뇌부종(腦浮腫)과 Aquaporins 발현에 미치는 영향
손낙원 ( Nak Won Sohn ),이주용 ( Ju Yong Lee ),구자승 ( Ja Seung Ku ),이동은 ( Dong Eun Lee ),신정원 ( Jung Won Shin ),김성준 ( Seung Joon Kim ) 대한본초학회 2010 大韓本草學會誌 Vol.25 No.4
Objectives: This study aimed at evaluation of the effects of Gastrodiae Rhizoma on brain edema and aquaporin water channel expressions in the brain. Methods: Brain edema following intracerebral hemorrhage (ICH) was induced by the stereotaxic intrastriatal injection of bacterial collagenase type VII in Sprague-Dawley rats. Then ethanol extract of Gastrodiae rhizoma was treated once a day for 3 days. Brain edema % and water contents, and cell size of neurons in the cerebral cortex were examined. Immuno-histochemistry was processed for AQP4, AQP1, and AQP9 expressions in the brain sections and area % of immuno-labeling was analyzed with image analysis. Results: 1. Ethanol extract of Gastrodiae Rhizoma reduced brain edema of ICH induced rats significantly. 2. Ethanol extract of Gastrodiae Rhizoma reduced excessive brain tissue water contents of ICH induced rats significantly. 3. Ethanol extract of Gastrodiae Rhizoma reduced cellular edema of neurons in cerebral cortex of ICH induced rats significantly. 4. Ethanol extract of Gastrodiae Rhizoma reduced AQP4 immuno-positive area % in cerebral cortex and external capsule of ICH induced rat brain significantly. 5. Ethanol extract of Gastrodiae Rhizoma reduced AQP9 immuno-positive area % in glia limitans externa of ICH induced rat brain significantly. Conclusions: These results suggest that Gastrodiae Rhizoma reveals protective effects against brain edema and cytotoxic edema of neurons by means of down-regulation of AQP4 expression in the brain.
면구에 미치는 側柏葉, 栢子仁의 效能에 關한 實驗的 硏究
손낙원,채병윤 慶熙大學校 1994 論文集 Vol.23 No.-
In order to evaluate the effect of Biotae Cacumen and Biotae Semen on acne experimentally, we studied the effects of water extracts on anti-inflammation(dextran and yeast paw edema) and anti-histamine, and alcohol extracts on stratum corneum of rabbit ear induced by oleic acid histologically. The results were obtained as follows: 1. Sample Ⅰ (Biotae Cacumen) and sample Ⅱ(Biotae Semen) were showed the significant decrease effect on the dextran paw edema as compared with control. 2. Sample Ⅰ was showed the significant decrease effect on the yeast paw edema, but sample Ⅱ was not significant as compared with control. 3. Sample Ⅰ and sample Ⅱ were not significant on the time to death induced by histamine shock as compared with control. 4. Sample Ⅰ was showed the significant decrease effect on the increased vascular permeability induced by histamine, but sample Ⅱ was not significant as compared with control. 5. Sample Ⅰ and sample Ⅱ were showed the epidermal regenerative effect on the stratum corneum induced by oleic as compared with control.
서울 居住主婦의 通過儀禮에 대한 意識과 儀禮飮食의 利用實態에 대한 硏究
손유미,정낙원,이효지 漢陽大學校 韓國生活科學硏究所 1992 韓國 生活 科學 硏究 Vol.- No.10
The purpose of this study is to investigate the actual situation of the transitional rites and to reestablish the meaning of that by grasping the relation among urban housewives' view, knowledge and behavior about transitional rites, by grasping the relation of those and background variables, by grasping the actual use -pattern of ritual food. The results are as follows 1.The view of the transitional rites shows significant difference according to educational level(p <0.001) and religion(p<0.001), and shows partial difference according to age, income, and residing shape. The older, the more traditional. With the less income, the more traditional. So do for the less educated, for Buddhist, and for those who live in a private residense. 2.The mean of knowledge about the transitional rites is 13.03. The knowledge shows significant difference according to religion, and shows partial difference according to educational level and income. the mean of knowledge is higher for the less educated, and for Buddhist. Those who earn more than 1500 thousand won have more knowledge than the other groups. 3.The behavior about traditional rites shows significant difference according to religion (p<0.001), residing shape(p<0.05), and shows partial difference according to age, educational level, family constitution, and whether employed or not. 4.There is close correlation(p<0.001) among the views, knowledge, and behavior. The behavior is traditional and the mean of knowledge is high as the view of the transitional rites is traditional.
뇌해마의 장기양 조직배양을 이용한 한약물의 뇌신경세포손상 보호효능 연구
정혁상,손낙원,이원철,Jung, Hyuk-Sang,Sohn, Nak-Won,Lee, Won-Chul 대한한방내과학회 2004 大韓韓方內科學會誌 Vol.25 No.3
Objectives : For the screening of neuroprotective effects of medicinal herbs, the complex system of animal models suffer some disadvantages in controlling critical parameters such as blood pressure and body temperature. Additionally, application of drugs to the appropriate brain area sometimes is difficult, due to poor permeability though the blood brain barrier, and so potential protective effects might be masked. Methods : Organotypic hippocampal slice culture (OHSC) method has the advantages of being relatively easy to prepare and of maintaining the general structure, including tissue integrity and the connections between cells. Drugs can easily be applied and neuronal damage can easily be quantified by using tissues and culture media. This study demonstrates neuroprotective effects of Puerariae radix (葛根, PR), Salviae miltiorrhizae radix (丹蔘, SR), Rhei rhizoma (大黃, RR), and Bupleuri radix (柴胡, BR). These were screenedand compared to MK-801, antagonist of NMDA receptors, by using OHSC of 1 week-old Sprague-Dawley rats. Oxygen/glucose deprivation (OGD) were conducted in an anaerobic chamber $(85%\;N_2,\;10%\;CO_2\;and\;5%\;H_2)$ in a deoxygenated glucose-free medium for 60 minutes. Water extracts of each herbs were treated to culture media with $5\;{\mu}g/ml$ for 48 hours. Results : Neuronal cell death in the cultures was monitored by densitometric measurements of the cellular uptake of propidium iodide (PI). PI fluorescence images were obtained at 48 hours after the OGD and medicinal herb treatment. Also TUNEL-positive cells in the CAI and DG regions and LDH concentrations in culture media were measured at 48 hours after the OGD. According to measured data, MK-801, PR, SR and BR demonstrated significant neuroprotective effect against excessive neuronal cell death and apoptosis induced by the OGD insult. Especially, PR revealed similar neuroprotective effect to MK-801 and RR demonstrated weak neuroprotective effect. Conclusions : These results suggest that OHSC can be a suitable method for screening of neuroprotective effects of medicinal herbs. (This work was supported by the research program of Dongguk University and Grant 01-PJ9-PG1-01CO03-0003 from Ministry of Health & Welfare.)
小續命湯이 中腦動脈 閉鎖에 의한 虛血性 腦損傷에 미치는 影響
趙英哲,孫洛源 慶熙大學校韓醫科大學韓醫學硏究所 1998 慶熙韓醫大論文集 Vol.21 No.1
This study demonstrates the effect of Sosokmyung-Tang on the ischemic damaged rat brain induced by middle cerebral artery (MCA) occlusion. 24 rats (Sprague-Dawley) were divided by three group : Sham operation group, MCA occlusion group, and Sosokmyung-Tang administration after MCA occlusion group. Dr. Chen's MCA occlusion model was used for this study. Ipsilateral common carotid artery (CCA) was occluded permanently prior to MCA occlusion. Right MCA was occluded permanently through the cranial window which was made by dental drill on the parietal bone 2mm rostral to the fusion of the zygomatic arch under the chloral hydrate anesthesia. Then contralateral CCA was occluded temporarily for 1 hour. Sosokmyung-Tang water extract (98mg/100g body weight) was administered orally at the 3 hours before and the 5 hours after the surgery. The MCA occluded animals were sacrificed at the 24 hours after the surgery. The brain was removed immediately, then 2mm thickness brain sections were made and stained with 2% triphenyl tetrazolium chloride(TTC) and 1% cresyl violet, infarct size was measured by using TTC stained brain sections with the computer image analysis system. The living neuron number and cell size in the penumbral area were measured by using cresyl violet stained brain sections. Sosokmyung-Tang administration did not reduce the infarct size significantly. There was significant decrease of the infarct size only in the interaural 6mm brain section with respect to that in the control group. The destruction of neurons, decrease of cell number and size, caused by MCA occlusion was attenuated significantly in the Sosokmyung-Tang administered animals. CA1 layer of hippocampus of the Sosokmyung-Tang administered animals was also less damaged than that in the control animals. From these results, it is presumed that Sosokmyung-Tang has a preventable effect on the ischemic brain damage, especially at the early stage.