石雄黃을 投與한 흰쥐에 蓄積되는 砒素量의 硏究

嚴東玉 又石大學校 1982 論文集 Vol.4 No.-

The purpose of this work is to review the absorption and accumulation of Arsenic after oral administration of Seok Woong Whang to rats. Experiments were carried out for the assay of accumulated Arsenic in blood, brain, each organ. The Arsenic concentration was studied by Silver diethyldithiocarbamate method and exhibited a increase by continous administration..

鹽酸프로메타진의 分光光度定量法에 關한 硏究

嚴東玉,徐恩實 又石大學校 1990 論文集 Vol.12 No.-

A rapid and convenient spectrophotometric method of the determination of promethazine hydrochloride and its unit dosage form is described. Extensive separation and extraction of the active ingredient are not required. The micro sensitive color response(入 max. 428nm) with palladium chloride reagent is the basis of the analytical technique. Color generation under standard conditions obeyed Beer's law between 5×10^-5mol∼2.5×10^-5mol(0.17∼0.87 absorbance, respectively, at 428nm) PMH. The relative standard deviation was found to be 0.73% It was also found that there is no interference from the commonly encountered excipients or additives such as diphenhy-dramine·HCL, caffeine anhydrous, sucrose, sodium saccharin, starch and citric acid. The results are reproducible.

鹽酸 클로르프로마진의 吸光度 定量法에 관한 硏究

嚴東玉 又石大學校 1988 論文集 Vol.10 No.-

A rapid and convenient spectrophotometric method of the detrmination of chlarpromazine hydrochloride and its unit dosage from is described. Extensive seperation and extraction of the active ingredient are not required. The microsensitive color response (入 Max. 435 nm) with palladium chloride reagent is the basis of the analytical technique. Color generation under standard conditions obeyed Beer's law between 1.0 × 10^-2㎎.㎖^-1-6.0×10^-2㎎·㎖^-1(0.17-095 absorbance, respectively, at 435 ㎚) The relative standard deviation was found to be 0.73%. It was also found that there is no interference from the commonly encountered excipients or additives such as glucose, lactose, sucrose, starch, talc and magnesium stearate. The results are reproducible.

Phenothiazine 유도체 중 Thioridazine. HCI의 定量

嚴東玉,姜兩承 又石大學校 1994 論文集 Vol.16 No.-

Generally, Phenothiazine derivatives are colored to form complex with palladium chloride. Using relationship of degree of coloring and concentration, studies on the determination of Thioridazine hydrochloride were performed. The results are followings. 1. Complex of TRH with palladium chloride has maximum absorbance at 430nm in 1, 2-dichloroethane solution. 2. Linear relationship was found between absorbance and concentration in the range of 0.5×10^-4∼2.5×10^-4M TRH. 3. The binding ratio of Thioridazine-palladium complex was presumed as Thioridazin-IM to palladium-1M by means of mole ratio and continious variation methods. 4. It was found that there is no interference from caffein anhydrous, corn starch, lactose, magnesium stearate.

사티오시아노코발트[Ⅱ]산 이온을 이용한 植物性鹽基成分의 確認 및 定量

嚴東玉,潘泰煥,韓相旭,申台容,都京三 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

A Coloring and spectrophotometric method using tetrathiocyanatocobaltate(Ⅱ) ion as a complexation reagent was developed for the identification of Coptidis Rhizoma and Phellodendri Cortex in herb medicine. Identification tests could be carried out coloring in test tube and ultraviolet-visible spectrophotometry. Coptidis Rhizoma and Phellodendri Cortex: with a solution of 1,2-dichloroethane in thst tube - yellowish green. The ultraviolet - visible spectrum of base components in 1,2-dichloroethane exgibits maxima at 270, 340, 442 and 624nm and was developed for the determination of berberine or palmatine in Coptidis Rhizoma and Phellodendri Cortex. Optimum conditions for good determination was determined: Maximum wavelength 442nm; Determination of berberine was possible to the range of 0.01mg/ml-0.10mg/ml in 1,2-dichloroethane. Calibration curve showed that the coefficient of variation was 0.999% for berberine. And determination of palmatine was possible to the range of 0.10mg/ml - 0.60mg/ml in 1,dichloroethane. The method was rapid, accurate. It was considered that spectrophotometry could be greatly applied to the analysis of base components in Coptidis Rhizoma and Phellodendri Cortex containing relatively small amount of gerb medicine extract.

Indole에 의한 DDVP의 螢光定量

嚴東玉,徐智榮 又石大學校 1991 論文集 Vol.13 No.-

The fluorescence spectrophotometric method is studied with regard to determination of DDVP in Organophosphorus pesiticides, which is applicable oxidation of Schoenemann reaction. DDVP is determined by fluorescence of indigo white, that is produced oxidation of indole by perphosphonate that produce at organophosphorus compound and peroxide. It examined the optimum reaction condition while DDVP reacted sodium perborate, trisbuffer and indole. It obtained maximum fluorescence wavelength, it examined with regard to effect of pH, effect of temperature and heating time, effect of Sodium perborate concentration, effect by solvent and effect of indole concentration on fluorescence of reaction product. Determination of DDVP was possible to the range of 10-20㎍/㎖. The method was applicable to the determination of DDVP in drug preparations. The fluorescence spectrophotometry was accuracy and precision is better than that of the absorbance spectrophotometry.

雄黃製劑 投與後의 殘留砒素量과 肝의 酵素活性化에 關한 硏究

嚴東玉,崔定容 又石大學校 1991 論文集 Vol.13 No.-

The purpose of this work is to review the absorption and accumulation of Arsenic and the effect of liver function by GOT, GPT activities after oral administration of Realgar Preparation to rats. Experiments were carried out for the assay of accumulated Arsenic in blood, heart, lung, liver, brain and kidney. The Arsenic concentration was determined by H.Gutzeit method and Silver diethyldithiocarbamate method. Activities of GOT and GPT in serum were measured by Reitman-Frankel method. The experimental results were as follows; 1. Realgar preparation decreased Arsenic accumulation after once administration than Realgar. 2. Realger Preparation decreased Arsenic accumulation after continuous administration than Realgar. 3. Activities of GOT and GPT in serum were increased but Realgar Preparation almost unchanged to liver function than Realgar.

玄胡索중 3級鹽基成分의 分離와 定量에 관한 硏究

嚴東玉,金基德,李來洪,梁在憲,申台容 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

A new application of specrophotometry for the isolation and determination of tertiary base in Corydalis Tuber was investigated. In order to establish the method for isolation and determination of tertiary base in the Corydalis Tuber. Tertiary base was isolated from Corydalis Tuber by organic solvent. When tertiary base was added to a solution containing cobaltous ion and excess thiocyan ion, yellowish-green, 1,2-dichloroethane-solube(base)_2Co(SCN)_4 precipitate. Supportive evidence for identification could be obtained by complexation with tetrathiocyanatocobaltate(Ⅱ) ion. The ultraviolet-visible spectrum of complex vompound in 1,2-dichloroethane exhibits maxima at 282nm and 624nm. The tertiary base was isolated from compex compound and identified as glaucine by UV-Visible spectrum and TLC. A tertiary base in 1,2-dichloroethane scanned from 200nm to 400nm qualitatively exhibits the same absorbance characteristics at identical wavelength as does isolated and concomitantly measured solution of a complex compound. The tertiary base components present in comples compound have been resolved on an absorbance solvent. And it is studied that the differential determination of tertiary base in Corydalis Tuber by spectrophometry. The tertiary base is determined by absorbance of colored products that is produced to reaction of tetrathiocyanatocobaltate(Ⅱ) ion under determination of tertiary base was possible to the range of glaucine 0.100mg/ml-1.000mg/ml in 1,2-dichloroethane. (maximum wavelength; 624nm) The calibration curve showed that the coefficient of variation was 0.9999% for glaucine. Also reproducibility tests showed that the coefficient of variation was 0.9999%. Arepid, specific and precise method using UV-Visible spectrophotometry and TLC has been developed for the isolation and determination of tertiary base in the Corydalis Tuber. The method was applicable to the isolation and determination of tertiary base in Corydalis Tuber or it's extract.

부자류 생약의 성분인 아코니틴과 관련 알칼로이드의 정량

엄동옥,한상욱,신현덕 대한약학회 2000 약학회지 Vol.44 No.2

Determination of Aconitum alkaloids in processed Buza (Cho-0, Salted Buza, Moist-heating Buza, Limed Buza), which had been prepared from the raw tubers of Aconitum chiisanenseb(Ranunculaceae), was established using visible spectrophotometry and high-performance liquid chromatography (HPLC) method especially for Aconitine analysis. Aconitum alkloids were reacted with tetra- thiocy-anatocobalt[II] complex ion to form a stable ion pair. The reaction product was insoluble in water but freely soluble in several organic solvents. 1.2-Dichloroethane was the best extracting solvent among the examined solvents. Spectrophotometry of Aconitum alkaloids at nax. 625 was carried out. The HPLC method for aconitine was carried out using Radial PAK-CN column with gradient solvent system by solvent mixture of acetonitrile and phosphate buffer (pH 3.0) at 4$0^{\circ}C$ and 254 nm. Linear relationship was found between absorbance response and concentration of aconitine in range of 0.45 mM~0.9 mM ($r^2$=0.9949) by spectrophotometry and 0.3 mM~1.2mM($r^2$=0.9983) by HPLC method. These methods have been found to be suitable and reproducible for routine analysis of Aconitum alkaloids and its pharmaceutical preparations.

吸光度測定에 의한 Triflupromazine·HCI의 定量

嚴東玉,韓相旭 又石大學校 1995 論文集 Vol.16 No.-

Generally, Phenothiazine derivatives are colored to form complex with palladium chloride. Using relationship of degree of coloring and concentration. studies on the determination of triflupromazine hydrochloride were performed. The results are followings. 1. Complex of triflupromazine with palladium chloride has maximum absorbance at 440nm in 1.2-dichloroethane solution. 2. Linear relationship was found between absorbance and concentration in the range of 1×10^-2∼6×10^-2㎎/㎖ TFP. 3. The binding ratio of triflupromazine-palladium Complex was presumed as triflupromazine-1mol to palladium-1mol by means of mole ratio and continuous variation methods. 4. It was found that there is no interference from 0.9_w/v%. NaCl, glucose, starch, sucrose, lactose, talc, magnesium stearate etc.