혈관 생성 억제 제를 투여한 신생마우스 폐 조직에서 Retinoic acid의 세포자멸사의 억제

주선영 ( Sun Young Ju ),조경아 ( Kyoung Ah Cho ),유경하 ( Kyung Ha Ryu ),우소연 ( So Youn Woo ),박은애 ( Eun Ae Park ),조수진 ( Su Jin Cho ) 대한주산의학회 2008 Perinatology Vol.19 No.1

목적: Retinoic acid는 폐포 재생에 중요한 역할을 하는 것으로 알려져 있어서 미숙아의 기관지폐이형성중의 예방에 사용되고 있다. 기관지폐이형성중의 병태생리는 미숙한 폐의 내피세포와 외피세포의 세포자멸사를 일으킴과 관련이 있다. 이에 본 연구에서 VEGFR2 억제가 신생 폐에서 세포자멸사를 증가시키는지, 또 retinoic acid의 투여가 본 폐 발달 억제 동물모형에서 세포자멸사를 억제하는지 알아보고자 하였다. 방법: VEGFR2 억제제인 SU1498을 생후3일된 마우스에 주사하고, 폐포발달이 이루어지는 그 후 10일간 retinoic acid나 위약을 주사하였다. 형태학적인 분석을 하였고, 세표자멸사를 비교분석하기 위해서 TUNEL 염색과 Annexin V을 표지자로 FACS출 시행하였다. 자멸사한 세포를 확인하기 위해서 동일초점 현미경으로 분석하였다. 결과: SU1498를 주사한 마우스에서 폐포면적과 평균폐포용적이 의미 있게 감소하였다. 세포자멸사도 약 3배 의미 있게 증가하였다. 자멸사한 세포는 내피세포와 상피세포에 분포하였다. Retinoic acid를 주사한 약 50%의 세포자멸사가 감소하였고, 형태학적으로 정상에 가까운 폐포 발달이 유지되었다. 결론: VEGFR2 억제를 하면 내피세포와 상피세포의 세포자멸사가 증가되면서 폐 발달이 저해되었다. 이어서 retinoic acid를 주사하면 세포자멸사가 억제되어 폐 발달유지에 도움이 되었다. 이러한 retinoic acid에 의한 세포자멸사의 억제가 기관지폐이형성증 예방에 기여할 것으로 추정된다. Background: Retinoic acid is known to play a role in alveolar regeneration and is used in the prevention of bronchopulmonary dysplasia (BPD) in premature infants. Many factors involved in the pathogenesis of BPD induce apoptosis of the endothelium and epithelium of the premature lung. We hypothesized that VEGFR2 inhibition would increase apoptosis in the newborn lung and retinoic acid would decrease apoptosis in our model of inhibited lung growth. Material and Methods: SU1498, a VEGFR2 inhibitor or vehicle was given to three-day-old mice. Subsequent retinoic acid or vehicle injection was given for ten days for the duration of alveolarization. Morphometric analyses were performed. Apoptosis was assessed with TUNEL staining and Annexin V staining. Co-localization of apoptotic cells with endothelial and epithelial cells was performed. Results: SUI498 injection reduced alveolar surface area and mean alveolar volume in newborn mice. Apoptosis was increased by three-fold in SU1498 injected mice. Apoptotic cells co-localized to endothelial and epithelial cells. Retinoic acid significantly reduced the degree of apoptosis by 50% in SU1498 injected mice and maintained lung development. Conclusion: VEGFR2 inhibition caused an arrest in lung development accompanied by an increase in apoptosis of endothelial and epithelial cells of the neonatal lung in mice. Subsequent retinoic acid treatment reduced apoptosis and we speculate that retinoic acid may preserve lung growth in bronchopulmonary dysplasia by inhibiting apoptosis in the neonatal lung.

Solar urticaria in Korean patients

( Sung Min Kim ),( Young Ah Cho ),( Ji Su Lee ),( Ji Youn Hong ),( Jin Hee Kim ),( Joo Ran Hong ),( Hye In Cheon ),( Yang Won Lee ),( Yong Beom Choe ),( Kyu Joong Ahn ) 대한피부과학회 2020 대한피부과학회 학술발표대회집 Vol.72 No.1

Background: Solar urticaria (SU) is an uncommon type of photodermatoses characterized by erythematous wheal with pruritus after sunlight exposure, which shows a female predominance. Only a few studies to analyze the clinical features of SU were reported in Asia. Objectives: We aimed to analyze the clinical and photobiological characteristics of SU in Korea. Methods: We retrospectively investigated the medical records of patients with SU who visited the department of dermatology, Konkuk university medical center from January 2005 to January 2020. Results: Of a total of 44 patients, 36 (81.8%) were male and 8 (18.2%) were female. The mean age at onset was 17.6 years (range, 5-47) and the mean threshold time was 19.7 minutes. Among 32 patients who underwent phototesting, the action spectrum most commonly implicated was ultraviolet (UV) A only in 12 (37.5%), and then visible light (415nm) only was responsible for SU in 8 (25.0%). Three patients (9.4%) were induced by both UVA and visible light (415nm). Even 4 patients (12.5%) were triggered by only natural sunlight. Serum immunoglobulin E levels were measured in 33 patients, and 17 (51.5%) showed values higher than normal. The treatment most widely used were oral antihistamine (43.2%), followed by phototherapy (13.6%). Conclusion: SU is a rare photodermatosis in Korea, which shows a male preponderance. In this study, UVA and visible light (415nm) were predominant action spectrum of SU.

노년기 주관적 연령과 건강노화와의 관계: 연령집단별 분석

최은영 ( Choi Eun Young ),조성은 ( Cho Sung Eun ),오영삼 ( Oh Young Sam ),장희수 ( Chang Hee Su ),김영선 ( Kim Young Sun ) 한국보건사회연구원 2017 保健社會硏究 Vol.37 No.1

본 연구의 목적은 노년기 주관적 연령(스스로를 노인 혹은 비(非)노인으로 인지하는지 여부)과 건강노화와의 관계를 연령집단에 따라 검증하는 것이다. 이를 위해 2014년 노인실태조사 대상자 중 연소노인(65~74세)과 고령노인(75~84세)을 연구대상으로 선정하였다. 연구의 주목적인 주관적 연령과 건강노화 간의 관련성을 검증하기 위해 연소노인 및 고령노인 집단별로 다중회귀분석(multiple regression analysis)을 실시하였다. 연구결과, 주관적 연령과 건강노화의 관계는 연령집단별로 다른 양상을 보이는 것으로 나타났다. 연소노인에서 주관적 연령이 비(非)노인인 경우, 높은 수준의 인지기능과 적은 만성질환 수, 낮은 수준의 우울, 그리고 높은 수준의 사회활동 참여를 보이는 것으로 밝혀졌다. 반면, 고령노인인 경우 주관적 연령은 인지기능을 제외하고 건강노화의 다른 세 요소와 유의미한 관련이 없었다. 본 연구는 주관적 연령을 건강노화의 새로운 고려요소로 제시하여, 두 요소의 관련성을 통합적인 차원에서 살펴보았다는 의의가 있다. 또한 노인을 연령대에 따라 구별된 집단으로 살펴봄으로써, 주관적 연령과 건강노화의 연관성이 연령집단마다 상이한 양상을 보임을 밝혔다는 의의를 가진다. The purpose of this study is to examine the relationship between subjective age (whether one subjectively perceives himself as `the elderly` or `not the elderly`) and healthy aging. Using data from the 2014 National Elderly Survey (KIHASA), this study selected a total of 9,653 participants consisting of young-olds (aged 65-74) and old-olds (aged 75-84) For both the young-old and the old-old, multiple regression analyses were conducted to examine the research model. In the young-old population, one`s considering oneself as “non-elderly” was significantly associated with higher cognitive functioning, fewer chronic diseases, lower depressive symptoms, and higher level of social engagement. On the other hand, in the old-old population, one`s considering oneself as “non-elderly” was associated only with higher cognitive functioning, and not with the other domains of healthy aging. The findings suggest that subjective age can be considered as an important predictors of older adults` healthy aging. These findings indicate that the association between subjective age and healthy aging has a different pattern depending on the age group.

용접공들의 면역능에 관한 연구

이수일,조봉수,김영욱,고광욱,조원근,김영실,강수용,황인경,조병만,김돈균 大韓産業醫學會 1997 대한직업환경의학회지 Vol.9 No.1

The authors examined human immunity indicators such as CD4, CD8, Natural Killer(NK) cell, IgG, IgA, and IgM, and heavy metals such as Pb, Cr, Cd to evaluate the health hazard of welders. We selected total 126 male subjects, 60 welders who had more than 5 year working history as an exposure group, and 66 office workers and medical college students as a control group. This immunity evaluation approach is the first newly designed study that has never been tried in past studies on welder's heath evaluation. And the results were as follows. 1. Total lymphocyte count of peripheral blood was significantly higher in welder group, 2,615±650 ea/㎕ compared with 2,368±681 ea/㎕ in control group(p>0.05). 2. The proportion of CD4, CD8, NK cell in welder group was 37.5±9.4%, 24.0±8.8%, and 21.0±9.5%, respectively, and 35.5±6.5%, 25.0±6.9%, and 19.6±8.8% in control group. CD4 and NK cell were slightly higher, and CD8 was slightly lower in welder group. but there was no statistical significance between two groups(p≥0.05). 3. IgG, IgA, and IgM in welder group were 15.9㎎/㎗, 2.7㎎/㎗ and 1.5㎎/㎗, respectively, and 15.6㎎/㎗, 2.9㎎/㎗ and 1.7㎎/㎗ in control group. There was no statistical significance between two groups(p≥0.05). 4. Blood lead, cadmium and chromium concentrations were 4.2±1.8㎍/㎗, 0.4±0.38㎍/㎗, 1.2±0.6㎍/㎗ in welder group and 3.2±1.7㎍/㎗, 0.44±0.29㎍/㎗, 1.1±0.6㎍/㎗ in control group, respectively, and these value were within normal ranges.

株當本數에 따른 油菜의 生育, 靑刈收量 및 組成分 變化

趙南棋,劉哲受,姜榮吉,宋昌吉,趙英一 濟州大學校 亞熱帶農業硏究所 1999 亞熱帶農業硏究 Vol.16 No.-

ABSTRCT Four rape cultivars were grown at 1 to 5 plants per hill to investigate the effects of the number of plants per hill on major agronomic characters of forage rape in Cheju volcanic soil. Days to flowering of Sparta was 191 days, those of Ramon and Youngsanyuchae were 189 and 188 days, respectively. And that of Hallayuchae was 180.3 days. Days to flowering decreased as number of plants per hill was increased. Sparta was tallest (153.6cm), while Hallayuchae was shortest (131.8cm). Plant height with three plants per hill( 155.3cm) was longest, while with 5 plants per hill(131.8cm) was shortest. The number of branches, stem diameter, leaf length and leaf width gradually decreased as the number of plants per hill were increased. Sparta had the greatest fresh and dry matter, total digestible nutrient (TDN) and crude protein yields, while Hallayuchae had the least. Fresh yield were greatest (7,544 kg) at the three plants per hill. Dry matter TDN, and crude protein yields were also greatest (7,544 kg) at the three plants per hill. Ramon had the greatist SPAD reading (44.7) while Youngsanyuchae had the lowest (40.7). SPAD reading were highest (44,1) in the one plant per hill plot but decreased as the number of plants per plant was increased. Sparta had greater contents of crude fiber and crude ash, but Hallayuchae and Youngsanyuchae had lower contents. The content of crude protein, crude fat, nitrogen free extract(NFE) and TDN were increased as number of plant per hill were increased. But the contents of crude fiber and crude ash were decreased as the number of plants per hill was increased.

상악동에 발생한 국균증의 치험2례

조재오,김영균,여환호,김수민,김수관 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.4

Aspergillosis of the maxillary sinus was rare disease, but it was increasing tendency with overuse antibiotics, streoid hormones, and anticanncerous agents. The clinical features and X-ray findings are similar to chronic suppurative paranasal sinusitis. Aspergillosis of the maxillary siuses may occur as a chronic diseae in an otherwise healthy person. This infection is usually confined to a single paranasal sinus, the maxillary antrum, though it can involve the orbit and may initiate with findings of proptosis and decreased vision. The disease is characterized by a wide range of initial symptoms, and should be considered as a possible diagnosis in sinusitis refractory to antibiotics and antral lavage. The choice of treatment of this disease is radical surgery and adjunctive systemic antifungal therapy. We experienced a case of right maxillary sinus aspergillosis in a 34 years old male who has dull pain on Rt. zygoma area, and a case of left maxillary sinus aspergillosis in a 30 years old male who had been suffered from nasal stiffness and frontal headache for 3-4 years. We reported two cases with review of literature.

지식 베이스의 에이젠트화에 의한 지식 미디어 시스템에 관한 연구

조정래,손영수,배상현 조선대학교 기초과학연구소 1996 自然科學硏究 Vol.19 No.1

The knowledge of the specialized fields has been changed rapidly in the both side of quantity and quality. A hyper media as a knowledge based system is so fixed in the linked media in mutual that we couldn't tell it information provision with the view of user. In this paper, we propose the way of offering intellectual and flexible information which is same with demand in the side of user, selecting, searching and composing the hypertext at the point of user's view through the design of knowledge media system. three concepts is used in order to challenge the knowledge media system : special document, agent system and ontology. The special document is a knowledge using special fields. The agent system is a homogeneous controller that operates on and application to ensure that its activities are coordinated with those of the others within the community, providing a uniform control mechanism. The last 3th concept, ontology is a language for knowledgement, which is a message exchanged among agents to ensure the proper interaction among them. The combination of these three concepts is used designed the prototype of knowledge media system.

기업이미지에 관한 小考 : 성호장생도라지 포장디자인 도입 사례중심으로

조용수,이영춘 진주여자전문대학 1998 論文集 Vol.21 No.-

The change of recognition about marketing is making important effects on the brand strategy and packaging design strategy. The case study in this article is describing technically elements of marketing, brand strategy and product from the development stage in new product till the exploitation stage in new market. The article in the following tries to make differences from the existing product through Niche marketing in the aspect of product positioning, high value and funiction in packaging design. This study takes the case from the beauty set of Sung-ho Jangsaeng Doraji developing and explaining the effect of sinergy as corporate image strategy, brand strategy and packaging design.

해외에서 유입된 정상 면역 성인의 Varicella 폐렴 1예

조영관,박은경,김양기,이영목,어수택,김태형 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Varicella-zoster virus, an unusual pathogen of community acquired pneumonia in adult, may causes respiratory distress and death. Since the first description by Waring et al. in 1942, the disease has been recognized by roentgenography, pathologic study, and virus studies as a special syndrome of the varicella virus. Immunocompromized conditions such as leukemia, malignant lymphoma and corticosteroid therapy predispose patients to varicella pneumonia. It occurs rarely in immunocompetent patients. The clinical manifestations of varicella zoster pneumonia exhibit a wide spectrum of disease from asymptomatic to death. Acyclovir is the recommended treatment and corticosteroid can be used in severe varicella pneumonia. We report a case of varicella pneumonia developed in a immunocompetent patient.

분리 유채단백의 전기영동 패턴에 미치는 Phytate의 영향

조희경,윤재영,이서래 이화여자대학교 생명과학연구소 1992 생명과학연구논문집 Vol.3 No.-

영양 저해인자로 알려진 phytate를 제거한 분리 유채단백(low-phytate rapeseed protein isolate)을 제조하여 유채단백질과 phytate와의 결합양상에 미치는 pH 및 phytate 첨가량의 영향을 알아보기 위하여 SDS-polyacrylamide gel 전기영동법을 수행 하였다. 가용화된 분리 유채단백은 pH2.0과 pH5.0에서 phytate 첨가량이 증가할수록 가용성 단백질 band의 수가 감소하였으며 pH11.5에서는 band의 변화가 없었다. pH2.0에서 대조군의 18개 band중 7개의 band(105.8,52.3,27.3, 34.8, 36.3, 21.3, 18.4 KDa)가 100mg phytate 첨가시 침전되었으며 150mg 첨가시에는 6개의 band (78.8, 46.5, 19.4, 16.8, 11.7, 8.5KDa)가 더 사라졌다. ph5.0에서는 대조군과 비교하여 15개의 band중 4개의 band( 34.8, 21.3, 18.4, 16.8KDa)만이 phytate 첨가시 침전되었다. 따라서 유채단백을 식품소재로 이용하기 위해서는 phytate 함량을 낮춤으로써 그의 기능성을 향상시킬 수 있을 것이다. This study was undertaken to investigate the effect of pH and phytate level on the solubility of the protein due to binding between phytate and low-phytate rapeseed protein isolate by means of SDS-polyacrylamide gel electrophoresis. Results showed that the number of protein bands decreased by the increasing amount of phytate added to the soluble extreact at pH 2.0 and 5.0 whereas there was no change at pH 11.5. Among 18 bands of rapeseed proteins at pH 2.0 seven bands (105.8, 52.3, 37.3,26.3,21.3,18.4 KDa) were removed by precipitation with 100mg phytate addition and six bands(78.8, 46.5, 19.4, 16.8, 11.7, 8.5KDa) further disappeared by 150mg phytate addition. Among 15 bands at pH 5.0, only four bands disappeared by phytate addition. It is suggested that thefunctionality of rapeseed protein isolate can be improved by lowering the phytate content.