Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

Isolation and Characterization of Antimicrobial Substance Macrolactin A Produced from Bacillus amyloliquefaciens CHO104 Isolated from Soil

( Seung Je Lee ),( Jeong Yong Cho ),( Jung Il Cho ),( Jae Hak Moon ),( Ki Deok Park ),( Young Ju Lee ),( Keun Hyung Park ) 한국미생물생명공학회 2004 Journal of microbiology and biotechnology Vol.14 No.3

凍結前處理가 굴의 冷凍變性 防止에 미치는 效果

趙權玉,趙永濟 釜山水産大學校 1978 釜山水産大學 硏究報告 Vol.18 No.1

凍굴의 凍結變性을 抑制하여 解凍時流出되는 drip量을 減少시키기 위하여 굴을 凍結前에 16種類의 添加物 溶液에 10分間 浸漬處理하여 凍結貯藏한 후 解凍하였을 때의 free drip量, expressible drip量, drip中의 全窒素量, DNA量, VBN量, 그리고 解凍後의 遊離水量 및 加熱 튀김減量 等을 定量하였다. 1) 試料의 個體에 따른 drip流出量은 個體가 작은 것이 큰 것보다 많았으며 處理液의 濃度가 높을수록 drip量이 적었다. 그리고 混合處理區가 單獨處理區 보다 drip流出量이 적었다. 2) drip中의 全窒素量은 大體로 drip流出量에 比例하였으며 混合處理液의 效果가 컸었다. 3) pH는 鹽處理區가 약간 低下하였고 燐酸鹽處理區는 7.0이상이었다. 4) 處理液의 濃度別 및 種類別에 따른 DNA의 變化는 drip流出量과 비슷한 경향을 나타내었다. 5) VBN은 食鹽處理區에서는 增加하였고 燐酸鹽處理區에서는 多少 減少하였으며 drip 流出量과는 반드시 比例하는 것은 아니었다. 6) Sucrose와 燐酸鹽處理區가 遊離水 流出抑制效果가 多少있었다. 7) 加熱튀김減量은 食鹽을 除外한 全處理區 모두 處理液의 濃度가 높을수록 적었다. It is well known that the frozen foodstuffs exude much drip on thawing. This undesirable drip loss is considered to be a measure of quality estimation and of the several factors affecting the drip loss, maintaining freshness of materials may be more important than any others. In the present paper, therefore, the amount of drip and its quality changes were tested in order to find out effect of pretreatment as means of maintaining freshness with several salt solutions on the denaturation of frozen oysters. After shelling, oysters divided into two groups as small and large were washed with fresh water and treated with solutions of sodium chloride, sucrose, poly-mixer(pyro-+tri-;1:1), mixed phosphates(ortho-+meta-+para-; 1:1:1) and these mixture, and then refrigerated at -30℃ by semi-air blast freezer. The total nitrgen, DNA, VBN, released water content and weight loss by cooking as well as the amount of free and expressible drips were also determined during 90 days storae at -25℃. As results of this experiments, the small oysters exude more drip than the large ones. The amount of total drip decreased as the concentration of solutions increased and it is similar to the total nitrogen and DNA in the drip. The amount of VBN increased in the sodium chloride treatment contrary to decrease in the case of phosphate. On the other hand, the pH of drip dropped slightly in treatment with sodium chloride and rose in case of phosphate. The water holding capacity of oysters was counterproportional to the drip loss and the effect of solutions used in treatment. The weight loss by cooking decreased in the all treatments except only one as the concentration of the solutions increased but sodium chloride exhibited adverse effect. It could be concluded from this study that pretreatments with solutions of some salts or sucrose would effective in maintaining the quality of frozen oysters.

노인정신장애 평가척도(Psychogeriatric Assessment Scale)의 한국판 표준화 연구

조맹제,박임순,신영민,김무진,정희연,정은기,최용성,조성진,서국희,함봉진 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.1

연구목적 : 본 연구는 치매와 우울증을 다차원적으로 평가할 수 있는 도구인 Psychogeriatric Assessment Scale(PAS)의 한국판(PAS-K)을 개발하기 위한 것이다. 방 법 : 번역, 역번역, 그리고 세차례의 예비조사를 통하여 PAS-K를 제작하였다. 60세 이상의 노인 291명(임상환자군 : 58명, 지역사회노인군 : 168명, 수용시설 거주자 : 67명)과 그들의 정보제공자들을 대상으로 PAS-K, Geriatric Depression Scale(GDS), Korean version of Mini-Mental State Examination(MMSE-K), Hamilton Rating Scale for Depression(HRSD), 그리고 Hachinski Ischemic Score 를 적용하고, Diagnostic Interview chedule(DIS-Ⅲ-R)을 사용하여 치매와 우울증을 진단하였다. PAS-K의 신뢰도와 타당도를 평가하고, 예민도와 특이도, Kappa값, 그리고 ROC커브 분석법을 이용하여 PAS-K의 6개의 소척도들(피검자면접 : 인지기능 장애척도, 우울척도, 뇌졸중척도, 정보제공자면접 : 인지기능 저하척도, 행동변화척도, 뇌졸중척도)의 최적 절단점을 구하였다. 결 과 : PAS-K는 높은 내적 일관성을 보였고, 검사자간 신뢰도도 만족할 만한 수준이었다. PAS-K의 소척도들을 다른 표준화된 검사도구들과 비교하였을 때 인지기능 장애척도와 인지기능 저하척도는 MMSE-K와, 우울척도는 GDS 및 HRSD와 유의한 상관관계를 보였으며, 뇌졸중척도는 Hachinski Ischemic Score와 통계적으로 의미있는 상관관계를 보였다. 최적절단점은 인지기능 장애척도 10점, 인지기능 저하척도 3점, 우울척도 5점, 피검자면접과 정보제공자 뇌졸중척도 모두 1점, 그리고 행동변화척도 2점으로 추정하였다. 결 론 : 본 연구를 통해 PAS-K의 신뢰도와 타당도가 검증되었고, 노인정신의학 역학연구나 보건분야에서 치매와 우울증의 임상경과관찰 및 일차 선별도구로서 유용하게 사용될 수 있게 되었다. Objectives : This study was designed to develop the Korean version of the Psychogeriatric Assessment Scale(PAS-K), a multidimensional screening tool for the dementia and depression. Methods : Through three times preliminary trials, the authors translated PAS into Korean. The PAS-K, Geriatric Depression Scale, Hamilton Depression Rating Scale, Hachinski Ischemic Scale and Mini-Mental State Examination-Korean version were administered to 291 subjects over the age of 60 and the same number of their informants(67 subjects were institutionalized, 168 subjects were in community, 58 subjects were psychiatric hospital patients). And the Diagnostic Interview Schedule(DSM-Ⅲ-R) was independently administered to exactly diagnose dementia and depression. The reliability and validity test, optimal cut-off point estimation for six each scale and ROC curve analyses were done to investigate the diagnostic validity of PAS-K. Results : Internal consistency and interrater reliability of the PAS-K were high. Concurrent validity of each scales of PAS-K was good in being measured with other standardized scales. The optimal cut-off points of each scale of the PAS-K were estimated as follows : 1) Stroke Scale of subject interview : 1, 2) Cognitive Impairment Scale of subject interview: 10, 3) Depression Scale of subject interview : 5, 4) Stroke Scale of informant interview : 1, 5) Cognitive Decline Scale of informant interview : 3, and 6) Behavioral Change Scale of informant : 2. conclusions : The PAS-K was valid and reliable screening tools for detecting dementia and depression. Therefore the PAS-K could be widely and extensively used in psychogeriatric epidemiological research or clinical setting of primary screening for dementia and depression.

인조 합성 테니스 바닥재의 지면 반력 분석

조승제,정미라,서국웅,박승범,윤양진,이훈식,강영택 釜山大學校 附設 體育科學硏究所 1998 體育科學硏究所 論文集 Vol.14 No.-

Some authors suggest that certain types of surfaces are the origin of such injuries. A few years after the first medical concerns about surfaces were voiced, publications of biomechanical measurements apperared, describing accleration, force, and impact measurement on different types of surfaces. In many sport activities, surfaces can be under very high dynamic load. This was the reason for the development of various methods for impact simulation, like the development of various methods for impact simulation, like the artificial athlete. Furthermore, it is important to collect information about the hardness of new and already existing surfaces in sport arenas. The idea on which this measuring system is based Is as follows: The stiffness of the material can be computed from the kinematics measurd at touch down of a rigid body onto a material sample. The results show the following The result for the artifical surfaces(Synpave ace) is surprising. It is known that these surfaces are much harder than synpave rebound classic, synpave spring. This finding suggests that it may be possible that the subjective impression is used as one criterion in the selection of landing(or style) strategies. The number of subjects in this experiment is too small to make statistically significant conclusions. It is shown analytically that when an object a deformable surface, the acceleration it experiences is inversely propotional to its mass. In future, it need to stress that the interaction between shoe and surface is important, and this aspect has now become well accepted. Considering biomechanical aspect in player's injury, it request Korean Standards for synthetic playing surfaces in sport like ASTM(America Society for Testing & Materials) standards of America, DIN 18035 standards of Germany, BSI standards of U.K.

玉田 M₃號墳 發掘調査 槪報

崔榮濟,金貞禮,朴升圭 嶺南考古學會 1988 嶺南考古學 Vol.- No.5

玉田古墳의 編年硏究

趙榮濟 嶺南考古學會 1996 嶺南考古學 Vol.- No.18

청소년기에 발생한 경부 지방육종의 치험례

조문제,강낙헌,김영진 大韓成形外科學會 1993 Archives of Plastic Surgery Vol.20 No.1

Liposarcomas are common malignant mesenchymal neoplasms, comprising approximately 15% of all soft-tissue sarcomas. They rarely occur in the head and neck region, the reported incidence being 2 to 6%. They make up only 4% of childhood soft tissue sarcomas, in contrast to adults, in whom they account for 15 to 20%. Less than 15 cases of liposarcomas in the head and neck have been reported in patients under the age of 18 years. Although the tumor is radiosensitive, wide surgical excision with postoperative radiotherapy is the treatment of choice at present. The prognosis is dependent of the type and grade. Five year survival is reported as over 80% for myxoid types and well-differentiated types but less than 50% for round cell types and pleomorphic types. We found a case of liposarcoma in the submandibular region in a 16-year-old female patient. In this case, surgical excision with postoperative radiotherapy was perfomed.

三角透窓高杯에 대한 一考察

趙榮濟 嶺南考古學會 1990 嶺南考古學 Vol.- No.7

高麗初期 鄕吏職의 由來에 對한 小考 : 鄕吏職名의 變化를 中心으로

趙榮濟 부산대사학회 1980 역사와 세계 Vol.- No.4