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외인성 Estrogen에 노출된 조피볼락, Sebastes schiegeli 치어의 혈장 VTG과 GPT의 변화
황운기,강주찬 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.3
Changes of plasma vitellogenin (VTG) and glutamate pyruvate transaminase (GPT) were examined for determining whether hepatocyte was damaged during the process of VTG induction in the juvenile rockfish, Sebastes schlegeli exposed to exogenous estrogen (estradiol-17β, E₂). Rockfishes were intraperitoneally injected with E₂(5 mg/kg B.W.) in 70% ethanol and plasma sampling were extracted at 0, 1, 3, 6, 9, 12, l5 days after E₂ administration. VTG and GPT were then analyzed by SDS-PAGE and Reitman-Frankel method, respectively. VTG band was detected at a molecular weight position of 175 kDa on Day 3 after E₂ administration. This band became more distinct at 6 days, but its was gradually thinned with time-course, and not detected at 15 days. GPT was suddenly increased at 1 days after E₂ administration and highest GPT was detected at 3 days. However, OPT was gradually decreased with time-course as the change of VTG. These results suggest that the process of VTG induction by exogenous E₂ damage to hepatocyte, and plasma GPT was temporarily increased in the juvenile rockfish.
TBT의 노출에 따른 넙치, Paralichthys olivaceus의 생존, 성장 및 산소소비의 변화
강주찬,황운기,지정훈 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.3
This study was carried out to examine the effects of his (tribytyltin)oxide (TBT), endocrine disrupting compounds (EDCs), on the changes of survival, growth and oxygen consumption rate in the oliver flounder, Paralichthys olivaceus. Oliver Flounders were exposed to sublethal concentration of TBT (0, 1.67, 3.20, 6.30 and 12.50㎍/L) during 6 weeks. Survival rate was decreased in a concentration and exposure period-dependent way and suddenly the reduction of more than 20% occurred at TBT concentration greater than 3.20㎍/L after exposure 6 weeks. Growth rate and feed efficiency significantly decreased at concentration greater than 3.20㎍/L. Oxygen consumption rate was also decreased in a concentration-dependent way and significantly decreased to 17, 48 and 67% than that of the control at the TBT concentration of 3.20, 6.32 and 12.50㎍/L, respectively. This study revealed that high TBT concentration (≥ 3.20㎍/L) reduced growth and oxygen consumption rates of the juvenile oliver flounder suggesting potential influence on the natural mortality of Paralichthys olivaceus in the coastal areas.
넙치, Paralichthys olivaceus 치어의 생존, 성장 및 산소소비에 미치는 수은의 만성적 독성
강주찬,황운기,지정훈,김성길,김재원 한국어병학회 2002 한국어병학회지 Vol.15 No.1
산업활동의 증가로 인해 해양에 유입되는 수은 (Hg)이 저서성 연안정착 어류인 넙치, Paralichthys olivaceus 치어의 생존, 성장, 사료효율 및 산소소비에 미치는 영향을 조사하였다. 6주 동안 0~0.13mg/L의 아치사 농도에서 넙치를 사육하며 Hg 독성에 대한 영향을 조사하였다. Hg 농도와 노출기간이 증가할수록 생존육은 감소했으며 6주 후, 0.05mg/L 이상의 농도 구에서 생존율이 급격히 감소했다. 성장률과 사료효율 또한, 각각 0.028mg/L (P<0.01)와 0.05mg/L (P<0.01) 이상의 농도 구에서 현저하게 감소하였다. 산소소비율은 0.05mg/L와 0.13mg/L의 농도구에서 각각 대조 구에 비해 25(P<0.01)와 32% (P<0.01) 감소해, 유의적인 차이를 나타내었다. 이상의 결과로부터 Hg 독성은 넙치의 성장, 사료효율 및 산소소비와 같은 생리적 기능을 저해해 생존율을 감소시키는 것으로 나타났다. Effect of mercury (Hg) toxicity on survival, growth, feed efficiency and oxygen consumption were examined in the juvenile olive flounder. Paralichthys olivaceus. Fishes were exposed to sublethal concentrations of Hg ranging from 0 to 0.13mg/L for 6 weeks. Hg reduced survival rate in a concentration and exposure period-dependent way and suddenly reduction occurred at Hg concentrations greater than 0.05mg/L after 6 weeks. Growth rate and feed efficiency also significantly decreased at greater than 0.028 and 0.05 mg/L respectively. Oxygen consumption rate was significantly decreased to 25 and 32% than that of the control at the Hg concentration of 0.05 and 0.13 mg/L respectively. These results suggest that Hg toxicity inhibit physiological function including growth, feed efficiency and oxygen consumption in the juvenile olive flounder, resulting in survival failure at high concentration.
유기주석 노출에 의한 Mysid, Neomysis awatschensis의 독성 영향
지정훈,김상규,황운기,강주찬 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.4
Tests for the toxicity of tributyltin (TBT) were conducted on mysid collected from Dadepo beach, Pusan, Korea. The toxic effects of tributyltin on the survival, growth and oxygen consumption of the mysid, Neomysis awatschensis have been evaluated. Mysids were exposed to several concentrations of TBT (0, 0.56, 1.15, 3.07 and 6.12 ㎍/L) for 6 weeks. Survival rate was decreased with increases in concentration and exposure time and the reduction of more than 40% occurred at TBT concentration greater than 1.15 ㎍/L after 6 weeks. Growth rate was significantly decreased at concentrations greater than 1.15 ㎍/L Oxygen consumption rate was also decreased in a concentration-dependent way and significantly decreased to 39, 47 and 69% of the control at 1. 15, 3.07 and 6.12 ㎍/L, respectively. These results indicate that the contamination of aquatic environment by TBT has the potential to significantly reduce coastal and estuaries recruitment of mysid.
Hwang, Un Gi 한국수산학회 2001 Fisheries and Aquatic Sciences Vol.4 No.4
Al and Cd-induced inhibition of vitellogenin (VTG) production was examined at the estrogen receptor (ER) level in rainbow trout Oncorhynchus mykiss hepatocytes. The binding of[^3H]estradiol-17β(E₂) to hepatocytes reached a plateau 3 days after addition of E₂(2×10^(-6)M) to the medium. The binding activity was linearly reduced with the increased concentrations (∼10^(-5) M) of 4-hydroxy-tamoxifen (4-OHT) and specific binding linearly increased with the increased doses of[^3H]E₂, indicating that the radioligand bound to ER. Al (10^(-4)) and Cd (10^(-6) M) as well as 4-OHT (10^(-6) M) significantly reduced the[^(3)]E₂- binding activity by 30∼40%, while they completely inhibited VTG production. Al and Cd had no effect on E₂-human ERα binding activity at any concentrations used (∼10^5 nM each). These results suggested that Al and Cd inhibited VTG production in part by interfereing with the ER level. Inhibitory effects of these metals on the E₂- dependent upregulation of ER activity are also discussed.
( Un Gi Hwang ),( Jung Sik Lee ),( Ju Chan Kang ) 한국수산과학회(구 한국수산학회) 2013 Fisheries and Aquatic Sciences Vol.16 No.4
The study was conducted to investigate the responses of the hepatic microsomal cytochrome P450 monooxygenase system in the rock bream Oplegnathus fasciatus after chronic exposure to 0, 1, 2, 4, and 8 μg/L tributyltin (TBT) concentrations for 4 weeks. Hepatic cytochrome 450 content and ethoxyresorufin O-deethylation (EROD) activity were found to significantly increase in fish treated with the higher concentration of TBT (≥4 μg/L); however, no significant changes were observed in penthoxyresorufin Odeethylation (PROD) activity in all treated groups compared to the control group. These findings suggest that exposure to a low TBT concentration (≥4 μg/L) has the potential to induce cytochrome 450 content and EROD enzyme activity in hepatic tissue inthe rock bream.
Hwang, Un-Gi,Lee, Jung-Sik,Kang, Ju-Chan The Korean Society of Fisheries and Aquatic Scienc 2013 Fisheries and Aquatic Sciences Vol.16 No.4
The study was conducted to investigate the responses of the hepatic microsomal cytochrome P450 monooxygenase system in the rock bream Oplegnathus fasciatus after chronic exposure to 0, 1, 2, 4, and $8{\mu}g/L$ tributyltin (TBT) concentrations for 4 weeks. Hepatic cytochrome 450 content and ethoxyresorufin O-deethylation (EROD) activity were found to significantly increase in fish treated with the higher concentration of TBT (${\geq}4{\mu}g/L$); however, no significant changes were observed in penthoxyresorufin O-deethylation (PROD) activity in all treated groups compared to the control group. These findings suggest that exposure to a low TBT concentration (${\geq}4{\mu}g/L$) has the potential to induce cytochrome 450 content and EROD enzyme activity in hepatic tissue in the rock bream.