Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

냉동 제대혈 세포의 체외 증폭

김삼용,김철희,배광봉,김현수,박상준,김종숙,윤환중,조덕연 충남대학교 암연구소 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Background : Cord blood(CB), which has no HLA restriction, is an alternative to bone marrow for hematopoietic stem cell transplantation. The use of cord blood, however, is limited by the number of progenitor/stem cells necessary to reconstitute the older child or adult. Therefore, ex vivo expansion of CB could have tremendous impact on diverse clinical settings. We studied the ex vivo expansion of isolated population of CD34_(+) cells from cryopreserved CB cells. Methods : CD34 cells were isolated from cryopreserved CB mononuclear cells. Purified cells were cultured with various combinations of hematopoietic growth factors including erythropoietin(EPO), stem cell factor(SCF), granulocyte-colony-stimulating factor(G-CSF), gra-nulocyte, macrophage-colony-stimulating factor(GM-CSF), interleukin-1β(IL-1β), 1L-3, and IL-6. After 7, 10 or 14 days of culture, the fold increases of colony-forming unit- granu-locyte, macrophage(CFU-GM), burst-forming unit-erythroid(BFU-E), colony-forming unit-mix (CFU-Mix), and high proliferative potential colony-forming cell(HPP-CFC) were evaluated. Results : Ten-day culture with the combination of EPO, SCF, G-CSF, IL-1β, and IL-3 resulted in a median of 60-fold increase of CFU-GM, which was greater than those with the combinations of less than 5 growth factors. The addition of IL-6 or GM-CSF to this combination did not enhance CFU-GM expansion. Ten-day culture was significantly superior to 7-day culture for CFU-GM expansion. Prolongation of culture to 14 days, however, revealed decreased expansion of CFU-GM compared to 10 days. BFU-E and CFU-Mix were expanded to 2~5 folds in 7-day culture with the combination of EPO, SCF, and G-CSF. Further expansion was not achieved in 10-day culture and colonies disappeared in 14-day culture. HPP-CFC was expanded to a median of 7.5 folds in 7-day culture with the combination of EPO, SCF, G-CSF, IL-1β, IL-3, and IL-6. Neither 10-day or 14 day-culture enhanced expansion of HPP-CFU. Conclusion : Cryopreserved cord blood cells maintain ex vivo expansion potential. In our system, 10-day culture with the combination consisting of EPO, SCF, G-CSF, IL-1β, and IL-3 seems to be adequate for hematopoietic progenitor/stem cell expansion from cryopreserved cord blood cells.

항암제 처리한 백혈병 세포주에서의 Apoptosis 발현

김삼용,윤소현,김현수,김종숙,윤환중,김진경,조덕연 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

The bcl-2 proto-oncogene encodes a 26 kD protein that promotes cell survival by blocking apoptosis. The bax protein is a member of the bcl-2 familly, now known to form heterodimers with the bcl-2 protein. The ratio of bax to bcl-2 is be critical in determining the fate of the cell in response to stimuli that can induce apoptosis. Extract of Pulsatilla Koreana (SB-31) showed promising antitumor activity in vitro with Topo I inhibitory action. In the present study, the relationship between apoptosis and the apoptosis related proteins, bcl-2 and bax were investigated in human leukemic cell lines HL-60, U-937 and CEM-CM3. All anticancer drugs(adriamycin, etoposide, camptothecin, SB-31) induced extensive apoptosis in HL-60, U-937 cells and CEM-CM3 cells. The expression of bcl-2 and bax protein were determined in cell lines by western blotting before and after incubation with anticancer drugs at different time points. 1) In HL-60 or U-937 cell lines, down regulation of bcl-2 and up-regulation of bax were found after incubation with ADR, VP-16 or camptothecin. 2) In HL-60 or U-937 cell lines, no significant change in bcl-2 or bax protein expression resulted after incubation with SB-31. 3) In CEM-CM3 cells, virtually no change was noted in bcl-2/bax expression after incubation with ADR, VP-16, camptothecin or SB-31. It is suggested that different leukemic cell lines use different pathways of apoptosis activation and a given cell may utilize different pathways of apoptosis activation in response to different cytotoxic agents.

항암제 처리한 백혈병 세포주에서의 Apoptosis 발현

김삼용,윤소현,김현수,김종숙,윤환중,김진경,조덕연 충남대학교 암연구소 1998 癌共同硏究所 硏究誌 Vol.2 No.1

The bcl-2 proto-oncogene encodes a 26 kD protein that promotes cell survival by blocking apoptosis. The bax protein is a member of the bcl-2 familly, now known to form heterodimers with the bcl-2 protein. The ratio of bax to bcl-2 is be critical in determining the fate of the cell in response to stimuli that can induce apoptosis. Extract of Pulsatilla Koreana (SB-31) showed promising antitumor activity in vitro with Topo I inhibitory action. In the present study, the relationship between apoptosis and the apoptosis related proteins, bcl-2 and bax were investigated in human leukemic cell lines HL-60, U-937 and CEM-CM3. All anticancer drugs(adriamycin, etoposide, camptothecin, SB-31) induced extensive apoptosis in HL-60, U-937 cells and CEM-CM3 cells. The expression of bcl-2 and bax protein were determined in cell lines by western blotting before and after incubation with anticancer drugs at different time points. 1) In HL-60 or U-937 cell lines, down regulation of bcl-2 and up-regulation of bax were found after incubation with ADR, VP-16 or camptothecin. 2) In HL-60 or U-937 cell lines, no significant change in bcl-2 or bax protein expression resulted after incubation with SB-31. 3) In CEM-CM3 cells, virtually no change was noted in bcl-2/bax expression after incubation with ADR, VP-16, camptothecin or SB-31. It is suggested that different leukemic cell lines use different pathways of apoptosis activation and a given cell may utilize different pathways of apoptosis activation in response to different cytotoxic agents.

단기배양을 통한 말초혈액 CD34 양성세포의 체외증폭

박상준,김철희,배광봉,김현수,김종숙,윤환중,조덕연,김삼용 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

Background: It is suggested that clinical practice in the areas of bone marrow transplantation and gene therapy might rely on the ex vivo expansion of hematopoietic stem/progenitor cells. However, the condition for ex vivo expansion of hematopoietic progenitor cells is not well established. The authors pursued a series of experiments to define the proper conditions for the expansion of hematopoietic cells in the short-term liquid suspension culture of mobilized peripheral blood CD34+ cells. Methods: 1.0ml cultures were initiated with 9×10^3 PB CD34+ cells, which were isolated from PB mononuclear cells (MNCs) by high-gradient cell sorting, in 12 well plates with the various combinations of hematopoietic growth factors(HGF). The following recombinant human HGFs were used: stem cell factor(SCF) 100ng/ml, granulocyte colony-stimulating factor(G-CSF) 100ng/ml, GM-CSF(granulocyte, macrophage colony-stimulating factor) 100ng/ml, interleukin-1 beta(IL-1B) 1ng/ml, interleukin-3(IL-3) 20ng/ml, interleukin-6 (IL-6) 100ng/ml. At the end of culture, colony-forming cells were evaluated by semisolid clonogenic assay. Results: 1) Using the high-gradient magnetic sorting system, CD34^+ cells were isolated with a yield of 40 3% 2) In 7 day culture of PB CD34^+ cells(9×10^3 cells), nucleated cells expanded mean 10×10^3(range, 9 to 20×10^3) with the addition of SCF alone, 35×10^3(range, 10 to 60×10^3) with SCF plus G-CSF plus GM-CSF, and 130×10^3(range, 40 to 300×10^3) with the combination of SCF, G-CSF, IL-1, IL-3, IL-6, GM-CSF. In 14 day culture, nucleated cells expanded 10×10^3 to 1,860×10^3 with combination of human hematopoietic growth factors. 3) In 10 day culture without medium change of PB CD34^+ cells, CFU-GM numbers expanded 16. 5 fold(range, 7 to 59 fold) with the addition of SCF plus G-CSF plus Il-1 plus IL-3, 31.3 fold(range, 20.5 to 101.1 fold) with the combination of SCF, G-CSF, IL-1, IL-3, GM-CSF. In 14 day culture with or without medium change of PB CD34^+ cells was inferior to 10 day culture for CFU-GM expansion. 4) There was no significant difference for CFU-GM expansion between five growth factors(SCF,G-CSF,IL-1,IL-3,GM-CSF) and six growth factors(five growth factors plus IL-6). Conclusion: The authors could confirm that short-term suspension culture of peripheral blood CD34+ cells could expand hematopoietic progenitor cells. Ten-day culture with medium change of CD34+ cells with the addition of five growth factors, i.e. SCF, G-CSF, IL-1B, IL-3, and GM-CSF, might be the most efficient in this system.

重化學工業機械의 國産化方案에 關한 硏究 : 特히 窯業에 있어서의 燒成爐, 粉碎機, 排風機, 冷却機, 電氣集마器 自動枰量供給器 等의 製作을 目的으로

趙哲衡,朴碩喆,丁太權,宋鐵,桭達福,金基玉,朴煥奎,趙煥從,朴善鐘,金種一,李茂錫 朝鮮大學校 1977 綜合論文集 Vol.1977 No.-

This is to investigate the posibility of home manufacturing of heavy chemical industry machines, such as rotary kiln, crusher, blower, cooler, electrostatic precipitator and weighing feeder of cement plant. It is concluded that even though we can not make all of them (some of them are made now and some others are going to be made in the near future, some of them are made whole and some others are made partially), we can build or export the cement plant by importing the important machines which we can not make now and by substituting them with ours gradually.

糖尿病 患者의 齒周狀態

金茂中,金錫煥 慶北大學校 齒科大學 1984 慶北齒大論文集 Vol.1 No.1

In order to obtain the basic data of periodontal treatment in diabetic patients, the periodontal status of 104 diabetic outpatients was compared with 127 other persons not having diabetes mellitus in Kyungpook National University Hospital, using Russell's periodontal index. It was found that: The severity of periodontal disease was significantly greater among the diabetic group(5.32±1.04) than among the non-diabetic group of patients(4.43±0.91). (p<0.001). Male periodontal status was the same as the female's in diabetic & control group. As age increased, the severity of periodontal disease increased significantly in diabetic and control group. The periodontal status of lower anterior teeth (5.82±0.84) was worst in diabetic group. The longer morbidity period in diabetic group, the worse periodontal status.(p<0.001).

생명공학의 윤리적 쟁점과 과학자의 책임

김환석 가톨릭대학교 인간학연구소 2004 인간연구 Vol.- No.6

지난 백여 년에 걸쳐 과학과 기술은 인간사회와 환경에 심대한 영향을 미쳐 왔다. 과학과 기술의 영향은 긍정적일 수도 있고 부정적일 수도 있다. 그러나 생명공학에서 볼 수 있는 바와 같이 최근의 급속한 과학의 산업화는 심각한 실제적 또는 잠재적인 위험을 수반해 왔고, 따라서 이에 대한 사회적 논쟁들이 초래되어 왔다. 특정한 기술을 개발할 것이냐 아니냐의 최종 결정은 물론 정부 또는 기업의 손에 달려 있으나, 그러한 기술을 연구하고 제공하는 담당자는 바로 과학자들이다. 따라서 과학자의 책임은 무거우며 그들의 연구 윤리는 매우 중요하다. 과학기술의 시대는 '위험 사회'로 특징지어지며 이는 일부 논자들이 주장하는 바와 같이 '성찰적 과학화' 또는 '탈정상 과학'을 요청한다. 이 후자의 개념들은 과학자가 전문가로서의 자신의 권위를 벗어 던지고 그 대신에 과학의 결과가 지닌 위험과 불확실성에 대해 일반 대중과 진지하게 대화하고 협력하는 것을 가리킨다. 이것은 결국 과학의 민주화가 필요하다는 것을 발하는 것이며, 나는 이것이 과학기술 시대에 요청되는 과학자의 연구윤리와 책임이기도 하다고 생각한다. Over the last one hundred years, science and technology have had profound effects on human society and the environment. The effects of science and technology may be either positive or negative. But the recent rapid industrialization of science, as can be seen in biotechnology, has heightened the actual and potential risks and has thus led to social controversies. Although the final decision to develop(or not) particular technologies may be in the hands of government or industries, it is scientists who do research such technologies and offer them. So the research ethics of scientists is very important. The era of science and technology is characterized by `risk society' which requires, as some writers named, `reflexive scientization' or `post-normal science.' Both of these concepts indicate that scientists should cast off their authority as experts and instead co-operate with the public to meet the risks and uncertainty of the consequences of science. This means the democratization of science, which is, in my view, also the research ethics required in the era of science and technology.

벼 재배방법과 규산질 비료 시용이 생육 및 도복에 미치는 영향

金正鎬,吳成煥,李哲遠,宋凡憲,孫錫龍 忠北大學校 農業科學硏究所 2002 農業科學硏究 Vol.19 No.-

본 시험은 벼 도목을 경감시키기 위한 재배대책을 강구하기 위하여 광안벼를 공시하고, 이앙재배와 담수직파재배에서 규산질 비료를 시용함에 따른 도복경감효과를 조사하였던 바 그 결과를 요약하면 다음과 같다 1 간장은 담순표면직파재배에선 규산시용구가 무처리보다 31cm 감소하여 37%가 단축되었으며, 기계이앙재배에서는 규산시용구가 무처리보다 5cm 감소하여 55% 단축되었다 2 하위절간장은 담수표면직파재배에서 규산시용구가 무처리보다 3.1cm 감소하여 36 9% 단축되었으며, 기계이앙재배에서는 규산시용구가 무처리보다12cm 감소하여 11% 단축되었다 3 좌절중은 담수표면직파에서 규산시용구가 73g이 증가하여 35 2%의 증가율을 보였고, 기계이앙재배에서는 규산시용구가 373g 증가하여 9.6%의 증가율을 보였다 4 수량은 담수표면직파재배에서 규산시용구가 무처리보다 30kg이 많아 64%의 증수효과를 나타내었고, 기계이앙재배에서는 규산시용구가 무처리보다50kg이 많아 9%의 증수효과를 나타내었다 This study was carried out to examine the responses of growth and lodging of rice as affected by both transplanting and direct seeding in waterlogging, which was differently applicated amounts of silicate fertlizers The Kwangan cultivar of Japonica type was used The growths including culm and internode lengths and grain yield were investigated in major growth stages and harvest time The culm length in waterlogging direct seeding was reduced in treatments of silicate fertilizers, about 37%, compared to that in the contro1 and it was reduced to 5.5% in the transplanting cultivation The breaking strength of lower internodes of rice were increased to 35.2% and 96% by applying of silicate fertilizers in the water direct seeding and in the transplanting cultivations, respectively The rice yields were increased with application of silicate fertilizers in both water direct seeding and transplanting cultivations compared to those of the control, about 64% and 90%, respectively.

충돌제트의 속도변화에 따른 실험적 연구

김동균,김정환,배석태,김시범,이영호 동아대학교 생산기술연구소 2001 生産技術硏究所硏究論文集 Vol.6 No.1

The flow characteristics of impinging jet flow are affected greatly by nozzle plate to distances. An sharp edge nozzle was used to achieve uniform mean velocity at the nozzle inlet, and its diameter is 10mm(d). Therefore, the flow characteristics on the impinging jet plate can be changed largely by the control of main flow. In the parent study, we investigate the effects of main flow velocity, its variable is nozzle inlet velocity(1.5m/sec, 3m/sec, 5m/sec)