전산화단층사진을 이용한 하악골 비대칭 환자의 저작근 평가

최순철,이선복,이진구,이원진,허민석,이삼선 大韓口腔顎顔面 放射線學會 2004 Imaging Science in Dentistry Vol.34 No.1

Purpose : To compare the size of the masseter and lateral pterygoid muscle between the affected and the unaffected side of the patients who have the chief complaints of the mandibular asymmetry. Materials and Methods : Twenty two patients (male: 4, female: 18, average age: 21.3 year-old) were radiographed using posterior-anterior (P-A) cephalography and computed tomography (CT). On P-A cephalography, the degree of deviation was determined by the distance from the mentum to the vertical reference line through the crista galli and the anterior nasal spine. On the scanned tracing papers of the maximum cross-sectional area of the masseter and lateral pterygoid muscle using axial CT images, the pixel number was measured. The ratio of the affected : unaffected sides were obtained. For the masseter and lateral pterygoid muscle, the relationship between the muscular volume and degree of skeletal hypoplasia was studied. Results : The half cases showed no skeletal asymmetry. The lateral pterygoid muscle of the affected side was larger significantly than unaffected side (p<0.05). However, there was no significant difference between two sides in the cases of skeletal asymmetry. There was only significant difference in the cases without skeletal asymmetry (p<0.05). Conclusions : To some extent, the slight mandibular hypoplasia could affect the growth of some masticatory muscles.

방사선조사 후 유표피암종세포내 칼슘농도의 변화와 apoptosis 발현에 관한 연구

이삼선,문제운,허민석,박태원,유동수,최순철 大韓口腔顎顔面 放射線學會 1999 Imaging Science in Dentistry Vol.29 No.1

Purpose: Ionizing radiations have been reported as an apoptosis initiating stimulus in various cells and it has established that sustained elevations in [Ca²+] can lead to DNA fragmentation by Ca²+-dependent endonucleases, ultimately resulting in apoptotic cell death. The previous experiments have been reported by using primarily thymocytes and lymphocytes and the change of [Ca²+] was measured only by minutes or hours respectively. We need to evaluate [Ca²+] in both several minutes and hours after irradiation of radiation of radiation therapy and verify the apoptotic cells. Materials and Methods: We have measured [Ca²+] in human gingival epitheloid cancer cell with 10 Gy irradiation, at minutely intervals and hourly intervals using digitized video-intensified fluorescence microscopy and the fluorescent Ca²+ indicator dye, fura-2. In order to find out that the transient rise in [Ca²+] could induced apoptosis, cells were incubated for 1 hour at 37℃ with TdT enzyme, rinsed and resuspended containing fluorescence and observed under a confocal fluorescence microscope. MTT assay was done to determine cell activity and LDH assay was done to determine the amount of necrotic cells. Results: After irradiation, the transient and temporal increasing of [Ca²+] in the KB cells was founded. Though, there was no change in the intracellular [Ca²+] at 30 minutes and 2 hours after irradiation. We could detect of DNA fragmented cells at 4 hours after 10 Gy irradiated cells. There were no significant differences between 4 hour, 1 day, 3 day cells. There were no significant differences in MTT and LDH assay between the irradiated group and the control group after 4 hours and 1 day. Though after 3 days there were differences in MTT and LDH assay between the irradiated group was significantly decreased than the control group, in LDH assay the number of necrotic cell death of the irradiated was higher than the control group. Conclusion: In KB cells there were incipient and temporal increasing of the [Ca²+] with 10 Gy irradiation and the apoptosis was founded from 4 hours later which was earlier than seeing of the change of the amount of the cellular ability and necrosis.(J Korean Oral Maxillofac Radiol 1999:29:109-117)

일차성 흉곽내 갑상선종 : 1예 보고

이미경,류대웅,이삼윤,최순호 원광대학교 의과학연구소 2009 圓光醫科學 Vol.24 No.1

Intrathoracic goiter consist of two types, namely, the primary intrathoracic goiter and secondary intrathoracic goiter. Primary intrathoracic goiters are very rare. Fifteen to fifty percent of these patients are asymptomatic. Symptoms, when present, are usually the result of tracheal or esophageal compression. Standard chest roentgenograms are often diagnostic but computed tomographic or radioactive iodine scans may be helpful. In symptomatic patients or those in whom explorations are undertaken for diagnostic purpose or exclude carcinoma, surgical removal is indicated. We report here on a case that was completely removed with using the thoracoscopic assist technique. The postoperative courses were uneventful.

악하선에 발생한 방선균증

이진호,박인우,최항문,허민석,이삼선 대한구강악안면방사선학회 2000 Imaging Science in Dentistry Vol.30 No.2

Actinomycosis is defined as a chronic, specific, suppurative, granulomatous disease caused mainly by the anaerobic, gram positive organism, Actinomyces israelii. Actinomycosis in the salivary gland is a rare disease that is caused by an inhabitant of the normal flora. We report the case of the actinomycosis of submandibular gland. A 53-year old man presented with the swelling on left submandiblar area. The lesion was not painful but had been increasing for about 10 days. In the CT view, the internal portion of the mass showed homogeneous moderate signal. The mass had continuities with the inferior portion of the left enlarged submandibular gland. In the MRI, there was a mass that showed a buldging pattern inferiorly in the left submandibular gland without bony invasion sign. The biopsy shows the colony of special organism. Many filaments are discovered with clubbed ends diffused from center of colony. We diagnosed this disease as actinomycosis in the submandibular gland by the postoperational biopsy. (Korea J Oral Maxillofac radiol 2000; 30: 132-137)

다량의 골양물질을 형성한 중심성 거대세포육아종의 영상진단

이설미,허민석,이삼선,최순철,박태원 대한구강악안면방사선학회 2000 Imaging Science in Dentistry Vol.30 No.2

A 19-year-old man was referred to Seoul National University Dental Hospital for evaluation of a large painless swelling of the left mandibular angle area in August, 1999. The growth had been first noted 6 years ago. He had visited other hospital in 1997. In spite of the treatment given at the hospital, the mass continued to grow rapidly. Conventional radiographs in 1999 showed an expansile, lobulated, and destructive lesion of the left mandibular body. CT scan demonstrated an expansile mass with a corticated margin. Bony septa were seen within the lesion. Internal calcification noted on the bone-setting CT image, and corresponded to the hypointense area in T1-weighted MRI image. MRI clearly delineated the extent of the lesion which had heterogenous intermediate signal intensity in T1-weighted images and heterogenous hyperintense signal intensity in T2-weighted images. The lesion was well-enhanced. Histopathologically, the lesion was well demarcated. Multinucleated giant cells were presented in a fibrous background, demonstrating a storiform pattern. Areas of osteoid rimmed by a few osteoblasts were scattered throughout the lesion. Inflammatory cells, blood vessels, and hemosiderin deposition were also shown. CGCG may show lots of internal calcification foci on the CT, and varied signal intensity in MRI. More cases will be needed to understand the features of the CT & MR fonding of CGCG. (Korean J Oral Maxillofac Radiol 2000 ; 30 : 127-131)

외상성 골낭의 방사선학적 연구

이건일,이삼선,최순철 大韓口腔顎顔面 放射線學會 1994 Imaging Science in Dentistry Vol.24 No.1

Fifty-two cases of traumatic bone cysts in 50 patients were analysed clinically and radiologically. The obtained results were as follows; 1. Women showed a slightly higher incidence than did men (56% of patients) and the average age proved to be 18.6 years. 2. The majority of the cases were asymptomatic, being detected incidentally, and over the half of the cases occurred in the mandibular symphyseal region. 3. All cases were unilocular and the largest diameter of the lesions varied from 1 to 10㎝, mean 3㎝. 4. Some degree of marginal condensation was present in 28 cases and 23 cases presented pencil-sketch appearance. 5. Many anatomical cortical plates(especially, mandibular inferior cortex and lamina durae) consisted of the margin of the lesions partly. 6. Erosive change of the mandibular inferior cortex was caused by 12 cysts, but cortical expansion only by 3 cysts including 2 cases of buccal expansion. 7. The lesion enveloped the roots of the adjacent teeth in 27 cases and scalloping was present between roots in 17 cases. 8. Lamina dura of the teeth was destroyed by only 1 cyst, and in 1 case root resorption was noticed. But there was no divergence of the roots of teeth.

방사선 조사가 배양된 조골세포의 apoptosis와 세포주기의 변화 및 석회화 결절 형성에 미치는 영향에 관한 연구

이영미,최항문,허민석,이삼선,최순철,박태원 대한구강악안면방사선학회 2000 Imaging Science in Dentistry Vol.30 No.3

Purpose : The study was aimed to detect the induction of apoptosis, cell cycle arrest and calcified nodule formation after irradiation on primarily cultured osteoblasts. Materials and Methods : Using rat calvarial osteoblasts, the effects of irradiation on apoptosis, cell cycle arrest, and calcified nodule formation were studied. The single irradiation of 10 and 20 Gy was done with 5.38 Gy/min dose rate using the 137Cs cell irradiator at 4th and 14th day of culture. Apoptosis induction and cell cycle arrest were assayed by the flowcytomtry at 1,2,3, and 4 days after irradiation. The formation of calcified nodules was observed by alizarin red staining at 1,3,10,14 days after irradiation at 4th day of culture, and at 1,4,5 days after irradiation at 14th day of culture. Results : Apoptosis was not induced by 10 or 20 Gy independent of irradiation and culture period. Irradiation did not induced Gl arrest in post-irradiated ostedblasts. After irradiation at 4th-day of culture, G2 arrest was induced but it was not statistically significant after irradiation at 14th-day of culture. In the case of irradiated cells at 4th day of culture, calcified nodules were not formed and at 14th-day of culture after irradiation, calcified nodule formation did not affected. Conclusion : Taken together, these results suggest that Irradiation at the dose of 10-20 Gy would not affect apoptosis induction of osteoblasts. Cell cycle and calcified nodule formation were influenced by the level of differentiation of osteblasts. (Korean J Oral Maxillofac Radiol 2000; 30: 189-198)

성공적인 임플란트시술을 위한 방사선검사

이삼선,최순철 대한구강악안면방사선학회 2005 Imaging Science in Dentistry Vol.35 No.2

Recently implant has become an important field in dental clinic. Radiographic examination of pre- and postoperation is essential for successful treatment. Clinicians should have knowledge about the purpose of the radiographic examination, suitable imaging modality for the cases, anatomic landmarks of tooth and jaw bone, advantage and limitation of panoramic radiographic examination for implant, principle and interpretation of crosssectional imaging, bone mineral density, post-operative radiographic examination. This paper will be helpful to get above informations for dentists who want to do dental implant successfully.

Saccharomyces uvarum의 인지질 생합성 및 지방산 조성에 미치는 탄소원의 효과에 관하여

이종삼,김선혜 성신여자대학교기초과학연구소 1988 基礎科學硏究誌 Vol.5 No.-

Saccharomyces uvarum을 30℃에서 Knopp's 최소배지에 자당결핍과 자당고농도 처리(15%)을 하여 배양시켰다. 이들 세포의 생장과 인지질 및 지방산 조성에 미치는 탄소원의 효과를 조사하기 위하여 배양 기간중 4, 7일 간격으로 일정량의 세포를 수확하여 생장률과 이에 따른 함량을 조사하였다. 자당 고농도 처리구는 배양 4일째에 생장감소 효과를 나타내었으며, 자당결핍구는 배양기간동안 세포생장을 관찰할 수 없었다. 주요 인지질의 지방산 palmitic acid와 oleic acid로 주로 이용되었으며, PC, PE에서는 대조구에 비하여 oleic acid가 조성상 급격한 이용의 증가를 나타내었다. Saccharomyces uvarum was cultured in the Knopp's media of the sucrose absence and the sucrose excess. In order to observe the effect of sucrose as carbon source on biosynthesis of phospholipids in these cells and the compositions of fatty acids, the cells were harvested at 4 and 7 days intervals of cultivations after inoculation. The growth rate at sucrose excess was decreased at 4 days of cultivation, but the growth rate of sucrose absence was not increase during whole period of cultivation. The fatty acids in major phospholipids were composed of palmitic acid and oleic acid. The amount of oleic acid in phosphatidylethanolamine and phosphatidylcholine was markedly increased to compared with those of the control.

치과에서의 방사선안전관리

이삼선 대한구강악안면방사선학회 2007 Imaging Science in Dentistry Vol.37 No.3

Although the diagnostic information provided by radiographs may be of definite benefit to the patients, the radio-graphic examination does carry the potential for harm from exposure to ionizing radiation. Therefore we should try to expose radiation as low as reasonably achievable and to give diagnostic information to patients as much as possible. All of dentists should have competence in radiation protection, I wish to deal with what we should do for the optimization of radiation protection in dental clinic.