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( Yunho Jang ),( Haet-nim Lee ),( Narae Kim ),( Yu Sin Ok ),( Tae-woon Kim ),( Suk Chan Jung ),( Jae-myung Kim ) 한국가축위생학회 2016 韓國家畜衛生學會誌 Vol.39 No.4
Mycobacterium avium subspecies paratuberculosis (MAP) causes Johne`s disease (JD) in ruminants. This is the first large scale report to estimate the herd-level prevalence of antibodies against MAP by using an ELISA to detect antibodies in bulk tank milk (BTM) samples. The samples were collected from January 2011 to November 2011, from 636 herds of the dairy farms in the Gyeonggi and Chungbuk areas of Korea. The overall apparent prevalence of MAP antibody-positive herds was 8.5%, and regional prevalence were 32/440 (7.3%) and 22/196 (11.2%) of dairy farms in the Gyeonggi and Chungbuk areas, respectively. The results did not differ significantly by region. While we have determined the prevalence rate of MAP in the Gyenoggi and Chungbuk areas in this study, there is a continuing need for well-designed studies to calculate the prevalence of MAP in dairy herds based on culture and molecular findings.
Jang, Yunho,Lee, Haet-Nim,Kim, Narae,Ok, Yu Sin,Kim, Tae-woon,Jung, Suk Chan,Kim, Jae-Myung The Korean Society of Veterinary Service 2016 韓國家畜衛生學會誌 Vol.39 No.4
Mycobacterium avium subspecies paratuberculosis (MAP) causes Johne's disease (JD) in ruminants. This is the first large scale report to estimate the herd-level prevalence of antibodies against MAP by using an ELISA to detect antibodies in bulk tank milk (BTM) samples. The samples were collected from January 2011 to November 2011, from 636 herds of the dairy farms in the Gyeonggi and Chungbuk areas of Korea. The overall apparent prevalence of MAP antibody-positive herds was 8.5%, and regional prevalence were 32/440 (7.3%) and 22/196 (11.2%) of dairy farms in the Gyeonggi and Chungbuk areas, respectively. The results did not differ significantly by region. While we have determined the prevalence rate of MAP in the Gyenoggi and Chungbuk areas in this study, there is a continuing need for well-designed studies to calculate the prevalence of MAP in dairy herds based on culture and molecular findings.
Diode Laser—Can It Replace the Electrical Current Used in Endoscopic Submucosal Dissection?
Yunho Jung,Gwang Ho Baik,Weon Jin Ko,Bong Min Ko,Seong Hwan Kim,Jin Seok Jang,Jae-Young Jang,Wan-Sik Lee,Young Kwan Cho,Sun Gyo Lim,Hee Seok Moon,In Kyung Yoo,Joo Young Cho 대한소화기내시경학회 2021 Clinical Endoscopy Vol.54 No.4
Background/Aims: A new medical fiber-guided diode laser system (FDLS) is expected to offer high-precision cutting withsimultaneous hemostasis. Thus, this study aimed to evaluate the feasibility of using the 1,940-nm FDLS to perform endoscopicsubmucosal dissection (ESD) in the gastrointestinal tract of an animal model. Methods: In this prospective animal pilot study, gastric and colorectal ESD using the FDLS was performed in ex vivo and in vivoporcine models. The completeness of en bloc resection, the procedure time, intraprocedural bleeding, histological injuries to themuscularis propria (MP) layer, and perforation were assessed. Results: The en bloc resection and perforation rates in the ex vivo study were 100% (10/10) and 10% (1/10), respectively; those inthe in vivo study were 100% (4/4) and 0% for gastric ESD and 100% (4/4) and 25% (1/4) for rectal ESD, respectively. Deep MP layerinjuries tended to occur more frequently in the rectal than in the gastric ESD cases, and no intraprocedural bleeding occurred ineither group. Conclusions: The 1,940-nm FDLS was capable of yielding high en bloc resection rates without intraprocedural bleeding duringgastric and colorectal ESD in animal models.
Prevalence of antibiotic resistance genes from effluent of coastal aquaculture, South Korea
Jang, Hyun Min,Kim, Young Beom,Choi, Sangki,Lee, Yunho,Shin, Seung Gu,Unno, Tatsuya,Kim, Young Mo Elsevier 2018 Environmental pollution Vol.233 No.-
<P><B>Abstract</B></P> <P>The wide use of antibiotics in aquaculture for prophylactic and therapeutic purposes can potentially lead to the prevalence of antibiotic resistance genes (ARGs). This study reports for the first time the profile of ARGs from effluents of coastal aquaculture located in South Jeolla province and Jeju Island, South Korea. Using quantitative PCR (qPCR), twenty-two ARGs encoding tetracycline resistance (<I>tetA</I>, <I>tetB</I>, <I>tetD</I>, <I>tetE</I>, <I>tetG</I>, <I>tetH</I>, <I>tetM</I>, <I>tetQ</I>, <I>tetX</I>, <I>tetZ</I>, <I>tetBP</I>), sulfonamide resistance (<I>sul1</I>, <I>sul2</I>), quinolone resistance (<I>qnrD</I>, <I>qnrS</I>, <I>aac(6′)-Ib-cr</I>), β-lactams resistance (<I>bla</I> <SUB>TEM</SUB>, <I>bla</I> <SUB>CTX</SUB>, <I>bla</I> <SUB>SHV</SUB>), macrolide resistance (<I>ermC</I>), florfenicol resistance (<I>floR</I>) and multidrug resistance (<I>oqxA</I>) and a class 1 integrons-integrase gene (<I>intI1</I>) were quantified. In addition, Illumina Miseq sequencing was applied to investigate microbial community differences across fish farm effluents. Results from qPCR showed that the total number of detected ARGs ranged from 4.24 × 10<SUP>−3</SUP> to 1.46 × 10<SUP>−2</SUP> copies/16S rRNA gene. Among them, <I>tetB</I> and <I>tetD</I> were predominant, accounting for 74.8%–98.0% of the total ARGs. Furthermore, <I>intI1</I> gene showed positive correlation with <I>tetB</I>, <I>tetD</I>, <I>tetE</I>, <I>tetH</I>, <I>tetX</I>, <I>tetZ tetQ</I> and <I>sul1</I>. Microbial community analysis revealed potential host bacteria for ARGs and <I>intI1</I>. Two genera, <I>Vibrio</I> and <I>Marinomonas</I> belonging to <I>Gammaproteobacteria</I>, showed significant correlation with <I>tetB</I> and <I>tetD</I>, the most dominant ARGs in all samples. Also, operational taxonomic units (OTUs)-based network analysis revealed that ten OTUs, classified into the phyla <I>Proteobacteria</I>, <I>Cyanobacteria/Chloroplast</I>, <I>Bacteroidetes</I>, <I> <U>Verrucomicrobia</U> </I> and an unclassified phylum, were potential hosts of tetracycline resistance genes (i.e., <I>tetA</I>, <I>tetG</I>, <I>tetH</I>, <I>tetM</I>, <I>tetQ</I> and <I>tetZ</I>). Further systematic monitoring of ARGs is warranted for risk assessment and management of antibacterial resistance from fish farm effluents.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We found prevalence of various ARGs from effluent of coastal aquaculture. </LI> <LI> Among the targeted ARGs, both <I>tet</I>B and <I>tet</I>D were predominant. </LI> <LI> <I>intI</I>1 had positive correlation with tetracycline and sulfonamide resistance genes. </LI> <LI> Network analysis revealed potential host bacteria for ARGs and <I>intI</I>1. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>