La traduction du theatre francais en Chine

( Yun Zhang Xiang ) 한국통역번역학회 2007 FORUM Vol.5 No.2

This article first presents a list of French dramas which have been translated into Chinese and performed in China. By analysing the list of works, the author attempts to nail down the factors which influence both the translation and the performance of French dramas in China. In addition to the selection of dramas made by the translators or the stage directors from the cultural perspectives, there are still specific requirements for the translated works to be performed on the stage in China. This article highlights the importance for the translation works to recover the performance implicit in the text of the original works. Meanwhile, the author explores the process of drama translation by taking examples of the Chinese translation of Le Tartuffe, the famous play written by Moliere.

Mechanism of a Torrential Rainstorm That Occurred to the West of a Mei-yu Frontal Low

Yun Zhang,Lifeng Zhang,Chunming Wang,Yanqiong Xie,Jie Xiang 한국기상학회 2014 Asia-Pacific Journal of Atmospheric Sciences Vol.50 No.4

Heavy rainfall usually occurs to the southeast of a Mei-yu frontal low. The rainstorms appeared to the west of Mei-yu frontal lows are difficult to forecast because their formation mechanisms are not well understood. An extreme rainfall event occurred to the west of a Mei-yu frontal low in Anqing City, Anhui Province, China, on 13 July 2010, which was not well predicted. Based on observation data, NCEP/NCAR reanalysis data and high-resolution numerical simulation output, the mechanisms of this severe rainstorm are analyzed in this paper. The results indicate that the eastward moving meso-α-scale low was a key synoptic system that resulted in the formation of the mesoscale convective system causing the rainstorm. As the low moved eastward and strengthened, the increasing northerly flow and the southerly warm/moist flow formed an eastwest elongated convergence line (frontal zone) at the west part of the low. While the low moved to the coastal region, its intensity was enhanced and its moving speed was reduced. As a result, the convergence line and the frontal zone remained quasi-stationary near Anqing. Furthermore, Anqing is located in a valley between the Dabie and Wannan Mountains; the northerly winds flowed around Dabie Mountains and entered the valley, leading to an intense local convergence and frontogenesis near Anqing. Under the unstable environments with sufficient water vapor, the local intense frontogenesis along with intense convergence triggered and maintained the quasi-stationary mesoscale convective system that resulted in the record-breaking rainfall in Anqing.

Genetic Analysis of the Liver Putative Tumor Suppressor (LPTS) Gene in Gastric Cancer

Hai Bing Zhang,Qu Zhang,Li Sa Wang,Fei Yan,Yun Lu,Zhi Qiang Wang,Jing Li,Liang Liang Zhang,Ying Yan Yu,Zheng Gang Zhu,Xiang Yin Kong,Lan Dian Hu 한국유전학회 2005 Genes & Genomics Vol.27 No.4

Regulation of Metabolic Flux in Lactobacillus casei for Lactic Acid Production by Overexpressed LdhL Gene with Two-Stage Oxygen Supply Strategy

( Xiang Yang Ge ),( Yan Xu ),( Xiang Chen ),( Long Yun Zhang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.1

This study describes a novel strategy to regulate the metabolic flux for lactic acid production in Lactobacillus casei. The ldhL gene encoding L-lactate dehydrogenase (L-LDH) was overexpressed in L. casei, and a two-stage oxygen supply strategy (TOS) that maintained a medium oxygen supply level during the early fermentation phase, and a low oxygen supply level in the later phase was carried out. As a consequence, a maximum L-LDH activity of 95.6 U/ml was obtained in the recombinant strain, which was over 4-fold higher than that of the initial strain. Under the TOS for L. casei (pMG-ldhL), the maximum lactic acid concentration of 159.6 g/l was obtained in 36 h, corresponding to a 62.8% increase. The results presented here provide a novel way to regulate the metabolic flux of L. casei for lactic acid production in different fermentation stages, which is available to enhance organic acid production in other strains.

Hamiltonian Stabilization Additional L2 Adaptive Control and Its Application to Hydro Turbine Generating Sets

Yun Zeng,Li-Xiang Zhang,Ya-Kun Guo,Jing Qian 제어·로봇·시스템학회 2015 International Journal of Control, Automation, and Vol.13 No.4

This study presents a design method for port-controlled Hamiltonian system, in which the nonlinear control is decomposed into the stabilization control at given equilibrium and additional L2 adaptive control. The stabilization controller includes the simplified object model, which is the internal model controlling the conception expansion in nonlinear case. The errors arising from parameters and modelling are evaluated using the weighing matrix of the penalty function, which simplifies the design procedure of control. The L2 adaptive control law is feedback control of the output errors with weighting matrix, which is the key character of the adaptive control. The proposed decomposing and simplifying method of the control law can be easily applied to investigate the higher order system. The procedure of control design and the issues associated with the application of control law are demonstrated by taking the hydro turbine generating system as an example. Simulation shows that the proposed method is very stable and robust.

Prefrontal cortex miR-29b-3p plays a key role in the antidepressant-like effect of ketamine in rats

Yun-Qiang Wan,Jian-Guo Feng,Mao Li,Mao-Zhou Wang,Li Liu,Xueru Liu,Xiao-Xia Duan,Chun-Xiang Zhang,Xiao-Bin Wang 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Ketamine has a rapid, obvious, and persistent antidepressant effect, but its underlying molecular mechanisms remain unknown. Recently, microRNAs (miRNAs) have emerged as important modulators of ketamine’s antidepressant effect. We investigated the alteration in miR-29b-3p in the brain of rats subjected to ketamine administration and chronic unpredictable mild stress (CUMS), and a sucrose preference test and forced swimming test were used to evaluate the rats’ depressive-like state. We used recombination adeno-associated virus (rAAV) or lentivirus-expressing miR-29b-3p to observe the change in metabotropic glutamate receptor 4 (GRM4). Cell culture and electrophysiological recordings were used to evaluate the function of miR-29b-3p. Ketamine dramatically increased miR-29b-3p expression in the prefrontal cortex of the normal rats. The dual luciferase reporter test confirmed that GRM4 was the target of miR-29b- 3p. The miR-29b-3p levels were downregulated, while the GRM4 levels were upregulated in the prefrontal cortex of the depressive-like rats. The ketamine treatment increased miR-29b-3p expression and decreased GRM4 expression in the prefrontal cortex of the depressive-like rats and primary neurons. By overexpressing and silencing miR-29b-3p, we further validated that miR-29b-3p could negatively regulate GRM4. The silencing of miR-29b-3p suppressed the Ca2+ influx in the prefrontal cortex neurons. The miR-29b-3p overexpression contributed to cell survival, cytodendrite growth, increases in extracellular glutamate concentration, and cell apoptosis inhibition. The overexpression of miR- 29b-3p by rAAV resulted in a noticeable relief of the depressive behaviors of the CUMS rats and a lower expression of GRM4. The miR-29b-3p/GRM4 pathway acts as a critical mediator of ketamine’s antidepressant effect in depressive-like rats and could be considered a potential therapeutic target for treating major depression disorder.

Aurantio-obtusin exerts an anti-inflammatory effect on acute kidney injury by inhibiting NF-κB pathway

Xiang Haiyan,Zhang Yun,Wu Yan,Xu Yaling,Hong Yuanhao 대한약리학회 2024 The Korean Journal of Physiology & Pharmacology Vol.28 No.1

Acute kidney injury (AKI) is one of the major complications of sepsis. Aurantio-obtusin (AO) is an anthraquinone compound with antioxidant and antiinflammatory activities. This study was developed to concentrate on the role and mechanism of AO in sepsis-induced AKI. Lipopolysaccharide (LPS)-stimulated human renal proximal tubular epithelial cells (HK-2) and BALB/c mice receiving cecal ligation and puncture (CLP) surgery were used to establish in vitro cell model and in vivo mouse model. HK-2 cell viability was measured using MTT assays. Histological alterations of mouse renal tissues were analyzed via hematoxylin and eosin staining. Renal function of mice was assessed by measuring the levels of serum creatinine (SCr) and blood urea nitrogen (BUN). The concentrations of pro-inflammatory cytokines in HK-2 cells and serum samples of mice were detected using corresponding ELISA kits. Protein levels of factors associated with nuclear factor kappa-B (NF-κB) pathway were measured in HK-2 cells and renal tissues by Western blotting. AO exerted nocytotoxic effect on HK-2 cells and AO dose-dependently rescued LPS-induced decrease in HK-2 cell viability. The concentrations of pro-inflammatory cytokines were increased in response to LPS or CLP treatment, and the alterations were reversed by AO treatment. For in vivo experiments, AO markedly ameliorated renal injury and reduced high levels of SCr and BUN in mice underwent CLP operation. In addition, AO administration inhibited the activation of NF-κB signaling pathway in vitro and in vivo. In conclusion, AO alleviates septic AKI by suppressing inflammatory responses through inhibiting the NF-κB pathway

HPLC Determination of Malondialdehyde in ECV304 Cell Culture Medium for Measuring the Antioxidant Effect of Vitexin-4"-O-glucoside

Xi-xiang Ying,Hai-bo Li,Zheng-yun Chu,Yan-jun Zhai,Ai-jing Leng,Xun Liu,Wen-jie Zhang,Ting-guo Kang,Chun Xin 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.7

To investigate the antioxidant effect of vitexin-4"-O-glucoside, a flavone glycoside, isolated from the leaves of Crataegus pinnatifida Bge. var. major, we developed a simple and sensitive high-performance liquid chromatography (HPLC) method to determine levels of malondialdehyde (MDA) in ECV304 cell culture medium after induction by tert-butyl-hydroperoxide (TBHP). The preparation of analyzed samples involved a one-step derivatization with thiobarbituric acid (TBA). HPLC analysis was performed on a SynergiTM Hydro-RP, a polar end-capped C18 column (250×4.6 mm, 4 μm), using an acetonitrile-ammonium acetate aqueous solution (10 mM, pH 6.8) as the mobile phase under linear gradient conditions with UV detection at 532 nm. The calibration curve was linear over 0.0125-1.25 μM MDA (r = 0.9951). Relative standard deviations (RSDs) of intra-day and inter-day precision were less than 6.1% and 5.0%, respectively. The mean recovery was 96.9 ± 1.6%. The lower limit of quantification (LLOQ) of MDA was 0.0125 μM. This chromatographic method was successfully applied to investigating the in vitro antioxidant effect of vitexin-4"-O-glucoside. Vitexin-4"-O-glucoside (120 M) protected ECV304 cells from peroxidation induced by TBHP.

Studies on the Ginseng Polypeptide - Decreasing Blood Sugar and Hapatic Glycogen

(Jin Zhang),(Hong Ying Zhang),(Wen Yun Du),(Da Wei Wang),(Ben Xiang Wang),(Ming Yang),(Yu Lian Jin),(Zhi Yong Cui),(Yan Wang) 고려인삼학회 1990 Proceedings of International Symposium on Korean G Vol.- No.-

Assessment of the Cytotoxic and Apoptotic Effects of Chaetominine in a Human Leukemia Cell Line

( Jing Yun Yao ),( Rui Hua Jiao ),( Chang Qing Liu ),( Yu Peng Zhang ),( Wan Guo Yu ),( Yan Hua Lu ),( Ren Xiang Tan ) 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.2

Chaetominine is a quinazoline alkaloid originating from the endophytic fungus Aspergillus fumigatus CY018. In this study, we showed evidence that chaetominine has cytotoxic and apoptotic effects on human leukemia K562 cells and investigated the pathway involved in chaetominine-induced apoptosis in detail. Chaetominine inhibited K562 cell growth, with an IC50 value of 35 nM, but showed little inhibitory effect on the growth of human peripheral blood mononuclear cells. The high apoptosis rates, morphological apoptotic features, and DNA fragmentation caused by chaetominine indicated that the cytotoxicity was partially caused by its pro-apoptotic effect. Under chaetominine treatment, the Bax/Bcl-2 ratio was upregulated (from 0.3 to 8), which was followed by a decrease in mitochondrial membrane potential, release of cytochrome c from mitochondria into the cytosol, and stimulation of Apaf-1. Furthermore, activation of caspase-9 and caspase-3, which are the main executers of the apoptotic process, was observed. These results demonstrated that chaetominine induced cell apoptosis via the mitochondrial pathway. Chaetominine inhibited K562 cell growth and induced apoptotic cell death through the intrinsic pathway, which suggests that chaetominine might be a promising therapeutic for leukemia.