Box-Wilson 계획법에 의한 沈降炭酸칼슘의 製造條件에 關한 硏究

김영윤,신화우,최광식,안세민,이광표,장영수 원광대학교 식품약품안전성연구소 1993 食品藥品安全性硏究 Vol.6 No.-

Medicinal Precipitated Clacium Carbonate is prepared by the interaction of Calcium Chloride and Ammonium Carbonate Solutions in this study. It has been known that the particle size of Precipitated Calcium Carbonate is greatly affected by the concentration, temperature, and moli-ratio of reactant solutions as well as the temperature at which the precipitate is dried and the temperature of washing water. The purpose of this study is to determine the optimum preparation Condition of medicinal Precipitated Calcium Carbonate. A randomized complete block design wuggested by G.E.P. Box and K.B.Wilson was for this purpose. The optimum preparation condition of light and fine precipitated Calcium Carbonate obtained from this study is as follows : 1) The reacting temperature range is 25∼45℃ 2) The concentration range of reactant solutions is 10∼30% 3) The optimum mole-ratio (CaCl/(NH) CO) is 1.0∼2.0. 4) The drying temperature range is 60∼80℃. 5) Temperature range of washing water is 25∼60℃.

일부 초등학생의 비만도에 따른 칼슘과 철의 섭취상태와 혈청 농도에 관한 연구

이화성,김은영,배윤정,승정자 숙명여자대학교 건강·생활과학연구소 2006 生活科學硏究誌 Vol.- No.22

The purpose of this study was to evaluate the nutrition status of calcium and iron in 116 elementary school students with different obesity index. Subjects were assigned to under weight, normal weight or over weight group according to the their obesity index. Their nutritional status, serum calcium and iron levels were evaluated based on 24-recall dietary recalls, anthropometric measurements and blood analysis. The mean height, weight, obesity index and blood pressure of total subjects were 150.4 cm, 45.5 kg, 5.8% and 118.2/76.8 mmHg. The mean % of body fat, SBP and WHR were higher while LBM and TBW were lower in over weight subject than those of other group. The iron intake of overweight was lower than that of underweight and normal subjects(p<0.05) and average calcium intakes were only 50% and 60% respectively of the RDA for Korean. Serum calcium and iron levels of a three groups were within the normal range. There were no significant differences in serum calcium and iron concentrations among three groups, however those of underweight group were a slightly lower than those of other two groups. There were significantly negative correlations between iron of intake and weight, obesity index, systolic pressure and body fat percent. Systolic blood pressure was negatively correlated to the intakes of energy, carbohydrate and protein. Energy intake was also negatively correlated to serum iron. To summarize the results, calcium and iron intakes should be supplied to elementary school students, especially those who were overweight. These indicated that overweight group had improper mineral nutrition status. Therefore, nutrition education and profound studies from many different focus for overweight groups are highly required.

중등도 심부전증 환자에서 Carvedilol의 치료 효과

차동훈,차영수,국진환,황경화,김형태,임상욱,김태용,조윤경,박필원 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.4

저체중 또는 과체중 청소년에서 캠프 프로그램을 이용한 영양 교육효과에 관한 연구

성미경,승정자,류화춘,박재년,박동연,최미경,조경옥,최선혜,이윤신,김유경,이은주 대한지역사회영양학회 2003 대한지역사회영양학회지 Vol.8 No.4

This study was performed to evaluate the effectiveness of a nutrition education program developed for nutritionally imbalanced adolescents. A summer nutrition camp was held for 23 overweight and 16 underweight subjects. Its effectiveness with regard to was evaluated at the end of the camp and 6 months later. Nutrition knowledge, nutritional attitude, food behavior, nutrient intake, exercise habit and ideal body figures desired by the subjects. The results showed significantly higher nutrition knowledge scores at the end of the camp as compared to those obtained prior to the camp program, and these scores were maintained for at least six months. Nutrition attitude scores also improved after the education program, and these improved scores also lasted for 6 months. However, the food behavior scores measured 6 months after the education program were not significantly different from those obtained prior to the camp. Also, the exercise habit, the ideal body figures and the body figures desired by the subjects remained unchanged. When nutrient intakes of subjects were assessed before the program and 6 months later, the mean daily vitamin C intake was significantly increased after the education program. Also, the intake of iron from plant food sources increased in the overweight subjects, while less iron from animal source were consumed by the underweight subjects. Both groups tended to consume more vegetables and fruits 6 months after the education program which may have contribute to the higher vitamin C and plant-based iron intakes. These results indicate that a 4-day nutrition education camp program sustained changes in nutrition knowledge and nutrition attitude for 6 months. The increased intake of vegetables and fruits was also achieved through this education program. However, changes in dietary behavior in adolescents may require repeated education.

T1 강조 영상에서 선조체 고신호강도를 보이는 당뇨 환자에서의 편측무도증

정승철,이병철,김상윤,배재천,황성희,도화범,박종호 대한신경과학회 1997 대한신경과학회지 Vol.15 No.1

Enhanced bath immersion vaccination through microbubble treatment in the cyprinid loach

Yun, Saekil,Giri, Sib Sankar,Kim, Hyoun Joong,Kim, Sang Geun,Kim, Sang Wha,Kang, Jung Woo,Han, Se Jin,Kwon, Jun,Oh, Woo Taek,Chi, Cheng,Jun, Jin Woo,Chang Park, Se ACADEMIC PRESS LTD 2019 FISH AND SHELLFISH IMMUNOLOGY Vol.91 No.-

<P><B>Abstract</B></P> <P>Immunization by bath immersion is likely the simplest method of fish vaccination. Although the route of immunogenicity has not been fully identified, immersion vaccination is clearly a useful labor-saving technique. In this study, microbubble (MB) treatment was assessed for its ability to improve the efficacy of bath immersion vaccination in the cyprinid loach. MBs are commonly defined as minute particles of gas with a diameter of less than 100 μm, which generated free radicals. Here, the efficacy of MB treatment for vaccination enhancement in the cyprinid loach was assessed in direct challenge experiments using the virulent <I>Aeromonas hydrophila</I> JUNAH strain; assessments comprised agglutination titer assay and non-specific parameter analysis. Agglutination titers were high in loaches that were immunized via injection with inactivated cells (FKC group); however, non-specific immune activation parameters (e.g., lysozyme, superoxide dismutase, and phagocytic activity) were more increased in loaches that were immunized via bath immersion with MB treatment. Moreover, MB-treated loaches showed comparable survival rates, relative to loaches immunized via injection with formalin inactivated cells. Thus, higher levels of non-specific immune parameters suggest increased efficacy of this vaccine approach. Improving the effectiveness of bath immersion vaccine will increase its affordability and ease of application in aquaculture.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Microbubble treatment was used for bath immersion vaccination in the cyprinid loach. </LI> <LI> Non-specific immune activation parameters were increased by microbubble treatment. </LI> <LI> Bath immersion with microbubble treatment was comparable to original injection vaccine in survival rates. </LI> <LI> This approach may increase vaccine efficacy, improving its use in aquaculture. </LI> </UL> </P>

Immunostimulation of <i>Cyprinus carpio</i> using phage lysate of <i>Aeromonas hydrophila</i>

Yun, Saekil,Jun, Jin Woo,Giri, Sib Sankar,Kim, Hyoun Joong,Chi, Cheng,Kim, Sang Geun,Kim, Sang Wha,Kang, Jung Woo,Han, Se Jin,Kwon, Jun,Oh, Woo Taek,Park, Se Chang Elsevier 2019 Fish & Shellfish Immunology Vol.86 No.-

<P><B>Abstract</B></P> <P>Over the last 50 years, various approaches have been established for the development of antigens for immunostimulation. We used phage lysate (PL), composed of inactivated antigens by the lytic bacteriophage pAh 6-c for <I>Aeromonas hydrophila</I> JUNAH strain to develop a vaccine for the prevention of <I>A. hydrophila infection</I> in <I>Cyprinus carpio</I> (common carp). We also assessed the poly <SUB>D,L</SUB> lactide-co-glycolic acid (PLGA) microparticles encapsulation method to increase the efficiency of the vaccine. Six groups of vaccines involving encapsulated by PLGA, formalin killed cells, or phage lysate at low or high concentration were prepared for intraperitoneal injection in <I>C. carpio</I>. Blood specimens and head kidney samples were collected at various time points for bacterial agglutination assay and to assess relative expression of immune-related genes interleukin-1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), lysozyme C, and serum amyloid A (SAA). The vaccine groups using high dose phage lysate antigen showed significantly higher agglutination titers than all other groups at 4- and 6-weeks post vaccination (wpv), with the titer of the PLGA encapsulated vaccine group being highest from 10 wpv to the end of the experiment. The survival rate of fish immunized with the phage lysate vaccines were higher than that of fish immunized with the formailin killed cells vaccine in the challenge experiment conducted 6 wpv. Additionally, the PLGA-encapsulated high dose phage lysate antigen vaccinated groups showed the best protective efficacy in the challenge experiment 12 wpv. Vaccines using the phage lysate antigen also showed higher IL-1β and lysozyme C gene expression at 7 days post vaccination (dpv) and 2 wpv, and higher TNF-α gene expression was seen at 7 dpv. Higher SAA gene expression was seen in these groups at 1 dpv. These results suggest that phage lysate antigen has the potential to induce robust immune responses than formalin killed cells-based vaccines, and could be more effective as a novel inactivated antigen in preventing <I>A. hydrophila</I> infection in <I>C. carpio</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Vaccine formulation using phage lysate (PL) antigen and PLGA encapsulation. </LI> <LI> Inactivated PL vaccines of <I>Aeromonas hydrophila</I> were evaluated in <I>Cyprinus carpio.</I> </LI> <LI> PLGA encapsulation is a low-cost, efficient antigen delivery system in aquaculture. </LI> <LI> Components of innate and adaptive immunity activated by PL antigen vaccine. </LI> <LI> PL could be an alternative for developing novel potent inactivated antigen in fish. </LI> </UL> </P>

Dietary factors related to body weight in adult Vietnamese in the rural area of Haiphong, Vietnam: the Korean Genome and Epidemiology Study (KoGES)

Kim, Sun-Hye,Hwang, Ji-Yun,Kim, Mi-Kyung,Chung, Hye-Won,Nguyet, Tran Thi Phuc,Kim, Wha-Young The Korean Nutrition Society 2010 Nutrition Research and Practice Vol.3 No.2

The objectives of this study were to examine the association between dietary factors and underweight and overweight adult Vietnamese living in the rural areas of Vietnam. A cross-sectional study of 497 Vietnamese aged 19 to 60 years (204 males, 293 females) was conducted in rural areas of Haiphong, Vietnam. The subjects were classified as underweight, normal weight, and overweight based on BMI. General characteristics, anthropometric parameters, blood profiles, and eating habits were obtained and dietary intake was assessed using 24-hour recalls for 2 consecutive days. A high prevalence of both underweight (BMI < 18.5 kg/$m^2$) and overweight (BMI ${\geq}$ 23 kg/$m^2$) individuals was observed (14.2% and 21.6% for males and 18.9% and 20.6% for females, respectively). For both genders, the overweight group were older than the under- and normal weight groups (P = 0.0118 for males and P = 0.0002 for females). In female subjects, the overweight group consumed significantly less cereals (P=0.0033), energy (P = 0.0046), protein (P = 0.0222), and carbohydrate (P = 0.0017) and more fruits (P = 0.0026) than the underweight group; however, no such differences existed in males. The overweight subjects overate more frequently (P = 0.0295) and consumed fish (P=0.0096) and fruits (P=0.0083) more often. The prevalence of both underweight and overweight individuals pose serious public health problems in the rural areas of Vietnamese and the overweight group was related to overeating and high fish and fruit consumption. These findings may provide basic data for policymakers and dieticians in order to develop future nutrition and health programs for rural populations in Vietnam.

Functionality Analysis on Tomato-fermented Soy Sauce

Yun-sun Kim,Eun-jung Yim,Seung-wha Jo,Eun-Ji Kim,Ho-Yeon Yang,Gwang-Su Ha,Sung Ho Cho,Do-Yeon Jeong 한국식품영양과학회 2016 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

EGFR-Mediated Reactivation of MAPK Signaling Induces Acquired Resistance to GSK2118436 in BRAF V600E–Mutant NSCLC Cell Lines

Kim, Sung-Moo,Kim, Hwan,Jang, Kang Won,Kim, Min Hwan,Sohn, Jinyoung,Yun, Mi Ran,Kang, Han Na,Kang, Chan Woo,Kim, Hye Ryun,Lim, Sun Min,Moon, Yong Wha,Kim, Joo Hang,Paik, Soonmyung,Cho, Byoung Chul American Association for Cancer Research 2016 Molecular Cancer Therapeutics Vol.15 No.7

<P>Although treatment of BRAF V600E-mutant non-small cell lung cancer (NSCLCV600E) with GSK2118436 has shown an encouraging efficacy, most patients develop resistance. To investigate the mechanisms of acquired resistance to GSK2118436 in NSCLCV600E, we established GSK2118436-resistant (GSR) cells by exposing MV522 NSCLCV600E to increasing GSK2118436 concentrations. GSR cells displayed activated EGFR-RAS-CRAF signaling with upregulated EGFR ligands and sustained activation of ERK1/2, but not MEK1/2, in the presence of GSK2118436. Treatment of GSR cells with GSK2118436 enhanced EGFR-mediated RAS activity, leading to the formation of BRAF-CRAF dimers and transactivation of CRAF. Interestingly, sustained activation of ERK1/2 was partly dependent on receptor-interacting protein kinase-2 (RIP2) activity, but not on MEK1/2 activity. Combined BRAF and EGFR inhibition blocked reactivation of ERK signaling and improved efficacy in vitro and in vivo. Our findings support the evaluation of combined BRAF and EGFR inhibition in NSCLCV600E with acquired resistance to BRAF inhibitors. (C) 2016 AACR.</P>