백서 심근경색모델에서 시간경과와 경색의 크기에 따른 심자도의 변화

김미성(Mi Sung Kim),박영선(Young Sun Park),권순길(Sun Gil Kwon),지정훈(Jeong Hoon Ji),신종성(Jong Sung Shin),오광식(Kwang Sik Oh),양용모(Yong Mo Yang),연태진(Tae Jin Youn),김동운(Dong Woon Kim),조명찬(Myeong Chan Cho),이용호(Yong Ho 대한내과학회 2002 대한내과학회지 Vol.62 No.1

N/A Background: Magnetocardiogram (MCG), which records the changes of magnetic fields generated by the heart`s electrical activity, theoritically can provide unique data for clinical application. To date, MCG has been investigated only at a single time point after myocardial infarction (MI) with severe left ventricular dysfunction in rats. The purpose of the present study was to investigate sequential changes of MCG after MI and to evaluate effects of infarct size on MCG. Methods: Acute MI were induced by the permanent ligation of left coronary artery in 22 rats. Magnetic fields were recorded just above a rat with Nb Superconducting Quantum Interference Device (SQUID) gradiometer inside a magnetically shielded room. MCG was measured before and immediately after surgery and it was subsequently recorded at the time points of 1, 4 and 6 hours post operatively. MCG was also measured at 1, 3, 7 and 21 days after surgery. Results: Elevation of ST segment and appearance of pathological Q wave on the MCG were evident immediately after the ligation of coronary artery and persisted to 6 hours after MI. On MCG, ST segment was depressed and T wave was inverted from 1 day after MI. In rats with small-and moderate- sized MI (infarct size〈30%), ST depression returned to near the isoelectric level and Q wave disappeared from 7 days after MI. However, ST depression and Q wave were still present in rats with larger infarct (infarct size≥30%). Conclusion: Evolutional changes of MCG were well-recognized up to 21 days after MI. Furthermore, the infarct size can be expressed by the extent of Q wave and ST segment depression on MCG. Taken together, these data indicate that MCG is a helpful modality for the diagnosis, evaluation of infarct size and follow up after MI.(Korean J Med 62:42-48, 2002)

심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

심근경색의 위험인자로서 Thrombomodulin유전자변이의 의의

박현영,김영미,권혁문,지선하,조승연,정남식,심원흠,장양수 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.6

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

대동맥 협착증과 동반된 출혈성 위장관 동정맥 기형에서 Estrogen-Progesterone 치료 1예

김도연,김화정,김효정,남준식,박수영,박영미,신정은,주미순,황민규,정혜경,손혜영,이선영,이한주 大韓消化器內視鏡學會 1999 Clinical Endoscopy Vol.19 No.부록2

교양소설의 두 유형에 나타난 교양주체의 의미

김미영 ( Mi Young Kim ) 한국언어문화학회 2015 한국언어문화 Vol.0 No.56

This paper presents an attempt to “re-read” The Square by Choi In-hun and Portrait of the Days of Youth by Lee Mun-yeol from the perspective of bildungsromans. The plots and embodiments of subject of culture in both the novels are similar to those of German"Bildungsroman"and also exhibit enough Korean features. Culture builds characters as intellectuals and cultured individuals and thus becomes a criterion to show the cultural refinement of the upper class of the time. It also contains the policy and ruling ideology of the time. Subjects of culture ultimately seek after harmony with the world through an ego exploration process, but the results are sometimes unsuccessful. The processes and results can lead to the disillusive and romantic types of bildungsromans. While The Square by Choi In-hun is one of the representative works in the disillusive type of bildungsromans, Portrait of the Days of Youth by Lee Mun-yeol is one of the representative works in the romantic type of bildungsromans. In disillusive bildungsromans, the subjects of culture fail to narrow down the gap between the world and themselves and eventually face frustration. In romantic bildungsromans, the main characters secure their own worlds. In The Square, Lee Myeong-jun, who is capable of criticizing the contradictions of political and social reality, is one of the utmost intellectuals and cultured men of the time, but he has a feeling of disappointment due to the gap between his own ideal and the reality and chooses to take his own life. In Portrait of the Days of Youth, Lee Yeong-hun, on the other hand, strengthens his own artistic world through ego exploration and achieves harmony with the world. Here one cannot disregard the gender differences in culture building between male and female subjects of culture. While male subjects of culture build their culture through"books"both in disillusive and romantic bildungsromans, their female counterparts build their culture by the influence of male subjects rather than on their own. In The Square, the female subject is perceived as the object of abasement by the male subject rather than being embodied as a perfect cultured person. The bildungsroman by Lee Mun-yeol, however, shows different aspects from the bildungsroman by Choi In-hun, embodying female subjects of culture in two types. They either receive instructions for culture building from the male subject or become subjects of culture through their own abilities. The former become the objects of criticism to male subjects like in The Square, the latter are set as helpers that exert influences on the ego exploration process of male subjects. Those changes to female subjects are the results of accepting the changes to the consciousness of culture.

Mycobacterium intracellulare에 의한 요추부 척추염 1예

장은영,김미영,김정욱,송은희,백관미,정용필,성흥섭,김미나,김양수,우준희,이상호 대한감염학회 2007 감염과 화학요법 Vol.39 No.3

비결핵성 미코박테리아에 의한 척추염에 대한 증례 보고가 국내에서는 아직 없었다. 저자들은 기저질환이 없는 환자에서 M. intracellulare에 의한 척추염을 rpoB 유전자에 대한 PCR-RFLP 방법을 통해 진단하였고 수술적 치료와 3제 병합 항균 요법으로 성공적인 임상 경과를 보인 1예를 경험하였기에 보고하는 바이다. We report a case of vertebral osteomyelitis due to Mycobacterium intracellulare in a 60-year-old man without predisposing conditions. He was successfully treated with surgical excision of the inflamed tissue and combined antimycobacterial therapy consisting of clarithromycin, ethambutol, and rifampin. In this case, polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) based on the rpo B gene of cultured isolates allowed rapid identification of M. intracellulare. Although very rare, nontuberculous mycobacteria (NTM) should be suspected as a causative pathogen of vertebral osteomyelitis. To the best of our knowledge, this is the first case of vertebral osteomyelitis caused by NTM reported in the Korean literature.

Comparison of fall recurrence between young-old and old-old

Yujeong Kim,Mi Young Kim 한국간호과학회 2021 한국간호과학회 학술대회 Vol.2021 No.10

Aim: As the elderly population is increasing, fall-related problems are emerging as important health problems. This study aimed to identify factors related to recurrent falls in community-dwelling young-old (65-74 years old) and old-old elderlies (≥75 years). Method: Data from 2017 National Survey of Older Koreans were used, and a total of 10,044 subjects over the age of 65 were included in analysis. Χ2 test, ANOVA, and logistic regression were performed for data analysis. Results: In the young-old elderly, limitations in ADL (OR=2.25, 95% CI: 1.44~3.54), visual aids (OR=1.56, 95% CI: 1.18~20.7), cognitive function (OR=0.93, 95% CI: 0.90~0.97), suicidal thoughts (OR=1.69, 95% CI: 1.17~2.45), number of chronic diseases (OR=1.22, 95% CI: 1.13~1.32), and number of prescribed medications (OR=1.06, 95% CI: 1.02~1.11) were factors that affected fall recurrence. In the old-old elderly, spouse (OR=0.76, 95% CI: 0.59~0.98), national basic livelihood security system (OR=1.53, 95% CI: 1.05~2.20), exercise (OR=1.48, 95% CI: 1.14~1.91), limitations in ADL (OR=1.98, 95% CI: 1.46~2.69), visual aids (OR=1.54, 95% CI: 1.17~2.02), suicidal thoughts (OR=1.63, 95% CI: 1.10~2.43), number of chronic diseases (OR=1.18, 95% CI: 1.11~1.26), and Parkinson"s disease (OR=3.53, 95% CI: 1.83~6.79) were factors that affected fall recurrence. Conclusion: Through this study, it was observed that there were differences in factors related to recurrent falls between young-old and old-old elderlies. Based on the findings, it would be necessary to develop appropriate interventions in order to prevent recurrent falls.

조선시대 전쟁소설에 나타난 여성에 대한 기억과 침묵

김영미 ( Kim Young-mi ) 조선대학교 인문학연구원 2021 인문학연구 Vol.0 No.61

재난 서사를 대표하는 조선시대 전쟁소설 「강도몽유록」, 「최척전」, 「김영철전」, 「박씨전」을 ‘여성’과 ‘기억’의 관점에서 읽어보았다. 전쟁은 남녀 모두에게 참혹한 상처를 남기는 사회재난이지만 그 영향력은 여성에게 더 폭력적으로 다가온다. 본고는 이러한 문제의식을 가지고 조선시대 전쟁소설 속에 형상화된 여성의 목소리를 탐색해 보았다. 공식기억에서 배제된 여성들이 17세기 전쟁소설 속에서는 어떻게 형상화되고 있는지, 어떤 여성을 기억하고 있는지, 그 기억의 작동 원리는 무엇인지 살펴보았다. 결론적으로 17세기 조선시대 전쟁소설 속에서 여성들의 고통은 전장(戰場)의 주체가 아니라는 이유로 주변적인 것으로 처리되든지 아니면 ‘열(烈)’이라는 프리즘을 투과시켜 논의되는 것을 확인할 수 있었다. 꿈이라는 틀을 통해 죽어서야 말할 수 있었던, 그것도 자살로 정절을 지킨 여성들만이 말할 수 있었던 「강도몽유록」의 귀녀(鬼女)들이나 살아서 정절을 잘 지켜내고 말할 수 있는 자격을 획득한 「최척전」의 옥영 같은 여성이 있었다. 이 여성들은 살아서나 죽어서나 자신의 정절을 증명해야 했다. 정절이 증명된 여성들만 기억하고 기려서 15명의 귀녀들처럼 본인들의 슬픔을 맘껏 통곡할 수 있었고, 옥영처럼 온 가족이 행복해질 수 있는 자격을 가지게 된다. 또한 남성 중심의 서사 이면에서 전쟁의 폭력성을 소리죽인 울음으로 견뎌야 했던 「김영철전」의 아내들이 있었다. 이들의 고통은 문면(文面)에 드러나지 않고 김영철 뒤에서 침묵해야 했다. 후대 「김영철전」 한글 이본들에서 ‘여성 인물에 대한 서술 확장’이 이루어지는데, 이는 숨어서 고통당하는 아내들의 존재를 찾아내고 그녀들의 아픔에 공감하면서 새롭게 그녀들을 기억하고자 한 결과이다. 한편 「박씨전」은 실제적인 여성의 목소리를 대변한다기보다 ‘피화당(避禍堂)’이라는 환상적 공간을 통해 전쟁으로 인한 여성들의 통곡과 울음을 우회적으로 위무하고 있다. 이는 역설적으로 병자호란 당시 여성을 보호할 ‘피화당’ 같은 유토피아적 공간은 절대 존재하지 않았으며 포로로 잡혀간 수많은 부인네들의 통곡을 상기시킨다. 울음과 통곡만으로 전란의 참혹함 속에서 죽어간 원한은 사라지지 않는다. 하지만 이런 원한을 조금이나마 삭일 수 있는 것이 자유로운 감정 표출이다. 이런 측면에서 전쟁소설 속에 나타난 여성들의 숨죽인 울음과 대성통곡은 기억해야 할 중요한 한 부분이다. I’ve read Gangdomongyurok, Choicheokjeon, Kimyoungcheuljeon, Parkssijeon, the war novels of the Joseon period, which are representing disaster narrative, from the perspective of women and memories. War is a social disaster leaving horrible scar on both men and women, but its influence is much more violent toward women. Having a critical mind on this, it searched the women's voice embodied in the war novels of the Joseon Dynasty in this study. It examined how women excluded from official memory are embodied in 17th-century war novels, what kind of women they remember, and how the memories work. In conclusion, it was verified that the women's pain in the war novels in the 17th century Joseon was treated as peripheral because it was not the subject of the battlefield, or it was discussed by passing through a prism called ‘virtue(烈)’. There were women who could say only after death through the framework of dreams, at that, the women who kept their chastity by suicide could say, like the ghosts in Gangdomongyurok or Ok-young in Choicheokjeon, who obtained the right to speak as she kept her chastity during her life. These women had to prove their chastity on whether alive or dead. Since only women with proven chastity can be remembered and cherished, they could wail their sorrows as much as they could like the 15 ghosts, and they could be qualified to be happy for the whole family like Ok-young. Also, there were wives in Kimyoungcheuljeon, who had to endure the violence of war with muted tears behind the male-dominated narrative. Their pain shouldn't be revealed and be kept silent behind Kim Young-chul. It made the 'extension of narrative on woman character' in the later different editions of Kimyoungcheuljeon, and it is the result of finding the existence of wives who are suffering behind, and trying to remember them newly by sympathizing with their pain. Meanwhile, in Parkssijeon, it indirectly pacifies the wailing and crying of women caused by war through a fantastic space called ‘Pihwadang(避禍堂)’, rather than representing the actual voice of women. This, paradoxically, reminds that there was never a utopian space such as the "Pihwadang" where women could be protected during the Second Manchu Invasion but the wailing of many wives who were taken a prisoner. Only with crying and wailing, it can't erase the resentment that died in the horrors of war. However, the free emotion expression could repress the resentment even a little bit. In this respect, the women's soundless cries and loud wailing in the war novels are the significant part to remember.