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- 저자 : ( Youl Woong Chun ) (검색결과 5 건)
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Analysis of the Apoptotic Mechanisms of Snake Venom Toxin on Inflammation-induced HaCaT Cell-line
Chun, Youl Woong,Song, Ho Sueb Korean AcupunctureMoxibustion Medicine Society 2017 대한침구의학회지 Vol.27 No.6
Objectives : In this study, the roles of Interleukin (IL)-4 and Signal transducer and activator of transcription 6 (STAT6), which have been reported to play a role in the pathogenesis of inflammation and cancer, were evaluated in snake venom toxin (SVT)-induced apoptosis. Methods : Inflammation was induced in human HaCaT kerationocytes, by lipopolysaccharide (LPS; $1{\mu}g/mL$) or tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), followed by treatment with SVT (0, 1, or $2{\mu}g/mL$). Cell viability was assessed by MTT assays after 24 h, and the expression of levels of IL-4, STAT6, and the apoptosis-related proteins p53, Bax, and Bcl-2 were evaluated by western blotting. Electro mobility shift assays (EMSAs) were performed to evaluate the DNA binding capacity of STAT6. Results : MTT assays showed that inflammation-induced growth of HaCaT cells following LPS or TNF-${\alpha}$ stimulation was inhibited by SVT. Western blot analysis showed that p53 and Bax, which promote apoptosis, were increased, whereas that of Bcl-2, an anti-apoptotic protein, was decreased in a concentration-dependent manner in LPS- or TNF-${\alpha}$-induced HaCaT cells following treatment with SVT. Moreover, following treatment of HaCaT cells with LPS, IL-4 concentrations were increased, and treatment with SVT further increased IL-4 expression in a concentration-dependent manner. Western blotting and EMSAs showed that the phosphorylated form of STAT6 was increased in HaCaT cells in the context of LPS- or TNF-${\alpha}$-induced inflammation in a concentration-dependent manner, concomitant with an increase in the DNA binding activity of STAT6. Conclusion : SVT can effectively promote apoptosis in HaCaT cells in the presence of inflammation through a pathway involving IL-4 and STAT6.
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Woo, Jang Chun,Bae, Woong Jin,Kim, Su Jin,Kim, Sung Dae,Sohn, Dong Wan,Hong, Sung Hoo,Lee, Ji Youl,Hwang, Tae-Kon,Sung, Young Chul,Kim, Sae Woong 대한비뇨기과학회 2011 Investigative and Clinical Urology Vol.52 No.5
<P><B>Purpose</B></P><P>Muscle-derived stem cells (MDSCs) harvested from skeletal muscles have the advantage of providing easier access and do not pose the immunogenic risks of embryonic stem cells. We investigated the effect of intracavernosal transplantation of MDSCs on erectile function in rats with bilateral cavernous nerve injury.</P><P><B>Materials and Methods</B></P><P>Adult male white rats underwent experimentation in 3 groups: group I, sham operation; group II, bilateral cavernous nerve injury; group III, bilateral cavernous nerve injury with MDSC injection. MDSCs were harvested from the femoral muscle of rats and were then injected into the cavernosum. Survival of MDSCs and measurement of erectile function was studied after 4 weeks. We checked the intracavernosal pressure (ICP) and obtained penile tissue. The expression of cyclic guanosine monophosphate (cGMP) was analyzed.</P><P><B>Results</B></P><P>Four weeks after transplantation, PKH-26-labeled MDSCs were identified in the cavernosal tissues of group III. Peak ICP and the drop rate of group II were 52±8.7 mmHg and 34±6.5 mmHg/min, respectively, whereas peak ICP and the drop rate of group III were 97±15.6 mmHg and 17±4.9 mmHg/min, respectively, showing that erectile function improved after MDSC transplantation (p<0.05). The expression of cGMP was significantly lower in group II (21.9±5.8 fmol/well) than in group I and group III (70.2±10.3 and 58.9±10.5 fmol/well, respectively).</P><P><B>Conclusions</B></P><P>In a cavernous nerve injury rat model, intracavernosal transplantation of MDSCs showed acceptable survival of MDSCs as well as improvement of erectile function.</P>
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물과 백색폴리스티렌 팬텀에 의한 10 MV X-선 빔 선량계측
김종언(Jong-Eon Kim),차병열(Byung-Youl Cha),강상식(Sang-Sik Kang),박지군(Ji-Koon Park),신정욱(Jeong-Wook Sin),김소영(So-Yeong Kim),조성호(Seong-Ho Jo),손대웅(Dae-Woong Son),최치원(Chi-Won Choi),박창희(Chang-Hee Park),윤천실(Chun-Sil 대한방사선과학회(구 대한방사선기술학회) 2008 방사선기술과학 Vol.31 No.1
본 연구의 목적은 고체물등가팬텀을 사용하여 절대흡수선량을 측정할 때 물등가깊이에 비례되는 측정값을 보정하기 위한 보정인자를 구하는데 있다. 10MV X-선 빔에 대하여 백색폴리스티렌팬텀과 물팬텀에서 측정의 조건들은 선원 대 전리조 중심까지의 거리를 SAD 100cm로 고정하였고, 조사면 크기(field size)는 각각 10×10cm2, 20×20cm2를 사용하였으며, 깊이는 각각 2.3cm, 5cm, 10cm, 15cm를 사용한 것이다. 두개의 팬텀에 대하여 분당 400MU의 출력을 갖는 선형가속기로부터 100MU의 전달로 각각의 조사면 크기와 깊이들에서 3번 측정으로 취득된 전리의 평균값을 측정값으로 얻었다. 이 실험으로부터 보정인자와 TPR에서 퍼센트 편차는 각각 0.97%, 0.53% 이하를 얻었다. 따라서, 고체물등가팬텀을 사용한 절대흡수선량 측정 시에는 보정인자와 TPR에서 퍼센트 편차를 사용하여 보정을 행하면 높은 정확도를 얻을 수 있다. The purpose of this study is to get the correction factor to correct the measured values of the absolute absorbed dose proportional to the water equivalent depth. The measurement conditions in white polystyrene and water phantoms for 10MV X-ray beam are that the distance of source to center of ionization chamber is fixed at SAD 100cm, the field sizes are 10×10cm2, 20×20cm2 and the depths are 2.3cm, 5cm, 10cm, and 15cm, respectively. The mean value of ionization was obtained by three times measurements in each field size and depths after delivering 100MU from linear accelerator with output of 400MU per min to the two phantoms. The correction factor and the percentage deviation in were obtained below 0.97% and 0.53%, respectively. Therefore, we can get high accuracy by using the correction factor and the percentage deviation in in measuring the absolute absorbed dose with the solid water equivalent phantom.
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송영웅,손보식,전재열,최윤기,Song, Young-Woong,Son, Bo-Sik,Chun, Jae-Youl,Choi, Yoon-Ki 한국건설관리학회 2009 건설관리 : 한국건설관리학회 학회지 Vol.4 No.1
최근 정부 공사를 중심으로 기술경쟁이 가능한 설계시공일괄 입찰방식이 확대 적용되고 있는 추세이며 이러한 시장의 변화에 따라서 그 중요성이 더욱 증가할 것으로 예상된다. 일반적으로 설계시공일괄방식의 단위 건설사업 생애주기는 기획, 기본 설계, 입찰, 계약, 상세설계, 착공, 시공, 준공, 평가의 진행단계를 가지게 된다. 계약자의 측면에서 주무부서의 잦은 변화로 효율적인 프로젝트 관리와 일관성 있는 정보의 공유가 어려워지는 문제점이 드러나고 있다. 이러한 문제점을 해결하기 위해 기존의 주무부서에 따른 기능 중심의 관리 체계에서, 프로젝트 중심의 관리 프로세스 체계로의 전환이 필요하다. 본 연구에서는 이러한 필요성에 따라 단위 프로젝트의 진행단계별 관리 프로세스 모델 개발을 목적으로 하였다. 이를 위해서 설계시공일괄방식의 진행단계별 주요 관리 쟁점과 의사결정 사항, 업무 분석을 하였고, 진행단계별 관리 주제의 책임 및 권한과 주요 관리 포인트의 변화를 분석하여 체계화 하였다. 프로젝트 특성별 및 진행단계별 연결고리가 되는 핵심 정보를 정의 하고 정보의 흐름을 분석하였으며, 이를 바탕으로 최종적으로 발주계획 수립 단계부터 실시설계 심의 단계까지의 업무 프로세스를 모델링하고 각 수행 주체별 업무프로세스를 모델링 하여 단위 프로젝트의 진행단계별 관리 프로세스 모델을 개발하였다. In public project market, design-build projects have been increased because of market trend change which enables technical competition. In this condition, its importance is also getting important. Generally, life cycle of design-build project consists of planning, preliminary design, procurement, contract, detail design, start of construction, construction, end of construction, and evaluation. From contractor's viewpoint, it has problems such as difficulties in project management and information sharing because of frequent change of charged division. To solve these problems, it is desirable to change from function-based management system to process-based management system. The purpose of this study is to develop management process model for design-build project by progress phases. This study analyzed main conflicts and decision making factors of each stage in design-build projects, then systemized management subjects' responsibilities and management points' change. And also this study defined the key information that is the key point by project characteristics and progress phases. Based on this analysis, this study did business process modeling from planning stage to construction design check stage. At last, we proposed the way to manage business process by design-build project progress.
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