심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

ATHB12, an ABA-lnducible Homeodomain-Leucine Zipper (HD-Zip) Protein of Arabidopsis, Negatively Regulates the Growth of the Inflorescence Stem by Decreasing the Expression of a Gibberellin 20-Oxidase Gene

Ora Son,허윤선,김윤경,이현정,김성한,김미란,남경희,이명석,김부용,박종범,박정안,이석찬,Atsushi Hanada,Shinjiro Yamaguchi,이인정,김승기,윤대진,Eva So¨derman,천충일 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

ATHB12, an ABA-lnducible Homeodomain-Leucine Zipper (HD-Zip) Protein of Arabidopsis, Negatively Regulates the Growth of the Inflorescence Stem by Decreasing the Expression of a Gibberellin 20-Oxidase Gene

Ora Son,허윤선,김윤경,이현정,김성한,김미란,남경희,이명석,김부용,박종범,박정안,이석찬,Atsushi Hanada,Shinjiro Yamaguchi,이인정,김승기,윤대진,Eva So¨derman,천충일 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

A transcervical amniotic fluid collector: a new medical device for the assessment of amniotic fluid in patients with ruptured membranes

Lee, Seung Mi,Romero, Roberto,Park, Joong Shin,Chaemsaithong, Piya,Jun, Jong Kwan,Yoon, Bo Hyun Walter de Gruyter GmbH 2015 Journal of perinatal medicine Vol.43 No.4

<B>Abstract</B><P>To describe a new device for the transcervical collection of amniotic fluid (AF) in patients with ruptured membranes, and to compare the concentration of proteins in fluid retrieved by transabdominal amniocentesis and the transcervical AF collector.</P><P>Paired AF samples were collected in patients with preterm prelabor rupture of membranes (PROM) (n=11) by transabdominal amniocentesis and with the transcervical AF collector (Yoon’s AF Collector™). Three proteins known to have high concentrations in AF [α-fetoprotein (AFP), β-human chorionic gonadotrophin (β-hCG), and prolactin] were measured.</P><P>(1) There was a significant correlation between the concentrations of analytes in AF obtained by transabdominal amniocentesis and by the transcervical AF collector (r=0.94, P<0.001 for AFP; r=0.96, P<0.001 for β-hCG; r=0.72, P<0.05 for prolactin); (2) Bland-Altman plots showed no evidence of heteroscedasticity between transabdominal or transcervical AF concentrations of these markers.</P><P>There was a strong correlation between the concentrations of proteins in AF collected by amniocentesis or with the transcervical device.</P>

대학생의 노인에 대한 태도에 관한 연구

이영희,권미경,김남선,김혜원,최미경,이규은,홍윤미 關東大學校 醫科大學 醫科學硏究所 2003 關東醫大學術誌 Vol.7 No.1

The purpose of this study is to identify the perception, the future and images, and prejudice toward old people. And to examine difference the group of gerontological education (educated group) with the non-educated group. 189 students in K university were participated in this study. The educated group was composed 101 students who enrolled liberal arts course that learned this title. Data were collected from May to June, 2002. The results are as follows; 1) Many students perceived the elderly that was started 60~64 years. 2) The perception, the future and images, and prejudice of both groups were some positively. 3) Each variables were not significantly difference. But the mean level of the educated group were more higher. 4) The educated group was found significantly related to the perception and prejudice(r=.303, p=.002). The non-educated group was found significantly related to the perception and the future and image(r=.421, p=.000), the perception and prejudice(r=.321, p=.003), the future, image and prejudice(r=.272, p=.012).

중량물 운반시 잡기유형과 부하단계에 따른 생리학적 연구

윤양진,이훈식,윤길환,정미라,이재규,이중숙,서국웅 釜山大學校 附設 體育科學硏究所 1999 體育科學硏究所 論文集 Vol.15 No.-

The purpose of this study was to examine physiological changes of the body according to various weight loads and the holding during carrying object. The experiments were conducted with utilizing physiological analyses for five male and five female adults as subjects. The results were as followed. 1. Any significant difference was not shown in heart rate, ventilation and amount of oxygen uptake of male subjects in the case of no load and 5kg. And also no significant difference was shown in expiratory exchange ratio of male subjects of no load, 5kg and 10kg, but in other measure items and other stages of weight was shown. 2. Significant differences were shown in heart rate, ventilation, defluxion of carbon dioxide, oxygen uptake per body weights, respiratory exchange rate, respiration rate and all other measure items of female subjects in all stages of load. 3. In the MMH job, there was no influence on male subjects up to 5kg loads, but there was an significant difference in male subjects in load walking from 5kg. It shows that even in 5kg weight MMH work, female subject's expiratory function was influenced. 4. 10kg load was the limit weight of female subjects and 15kg was of male subjects when they walked on treadmills with 4km/h velocity for 3 minutes, holding loads in the right and vertical types.

성인에서 장기간 Furosemide오용과 연관된 신수질 석회화

김윤구,이윤하,이규백,장세호,김대중,오하영,김보현,김미경,조윤숙 대한신장학회 1996 대한신장학회잡지 Vol.15 No.4

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

1인 미디어 이해와 제작 수업에 대한 초등교사의 자문화기술지

윤미 ( Yoon¸ Mi ),이지영 ( Lee¸ Jiyoung ) 한국독서학회 2021 독서연구 Vol.- No.60

이 논문은 초등교사가 1인 미디어의 이해와 제작 수업을 실천하면서 경험한 것, 성찰한 것을 자문화기술지 연구로 담아낸 것이다. 1인 미디어의 이해와 제작 수업은 ① 준비와 시작, ② 1인 미디어의 이해, ③ 1인 미디어의 제작, ④ 제작한 1인 미디어 공유로 이루어졌다. 수업 경험을 디지털 이주민-교사의 입장과 디지털 원주민-학생의 입장에서 성찰하였다. 교사는 ‘교사’로서 정체성과 ‘디지털 이주민’으로서의 정체성 사이에서 갈등한다. 학생은 디지털 원주민으로 주도적으로 수업을 이끌기도 하고 디지털 이주민과 협상하기도 한다. 교사와 학생은 1인 미디어 수업을 함께하면서 파트너 관계를 끊임없이 조정한다. 미디어 리터러시 교육은 미디에 대한 이해와 제작이 순환 구조로 이루어져야 한다. 1인 미디어의 이해와 제작 역시 재미나 흥미 위주의 활동으로 접근할 것이 아니라 미디어 리터러시 교육의 차원에서 접근-분석과 평가-제작-성찰-실천의 순환 과정으로 다루어지기를 기대한다. This paper describes the authentic experiences and reflections of the author, an elementary school teacher about understanding and production of personal media through autoethnography in their teaching class. The class on understanding and production of personal media covered ① preparation and beginning, ② understanding of personal media, ③ production of personal media, and ④ sharing of the media production. I reflected the classroom experience based on the digital immigrant teacher's perspective and the digital native students’ perspective in this paper. A teacher is in conflict between their identity as a ‘teacher’ and a ‘digital immigrant’. Students lead classes proactively and negotiate with a digital immigrant as digital natives. The teacher and students constantly adjust their partnership as they work together in personal media classes. In media literacy education, understanding and production of media should be done in a cyclical manner. I anticipate that understanding and production of personal media should not be approached merely as a fun or interesting activity, but should be treated as a cycle of access-analyze & evaluate-create-reflect-act in terms of media literacy education.