Dimethylnitrosamine 유발 급성 간 손상 흰쥐에서 ^(99m)Tc-Lactosylated Serum Albumin을 이용한 간 기능의 평가

정신영,이재태,서명랑,유정아,배진호,안병철,황재석,정재민,하정희,이규보 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.6

목적: ^(99m)Tc-lactosylated serum albumin (^(99m)Tc-LSA)은 간세포에 특이적으로 결합하는 간수용체 영상용 방사성의약품으로 새로이 합성되었다. 간섬유화를 유발하는 dimethylnitrosamine (DMN)을 투여한 간 손상 휜쥐 모델에서 ^(99m)Tc-LSa의 역동학적인 간섭취를 조사하고 간효소치의 변화와 조직학적 소견을 비교하여, LSA의 간섭취가 간기능의 변화를 반영하는지를 연구하였다. 대상 및 방법: SD계 흰쥐에 DMN를 27 mg/kg으로 복강 내 주사하여 급성 간손상을 유도하고 대조군과 비교하였다. DMN을 주사한 흰쥐를 3일(DMN-3), 8일(DMN-8), 21일(DMN-21)에 ^(99m)Tc=LSA (1,665 mg/kg) 29 MBq를 정맥 주사하여, 30분 동안 동적 영상을 획득하고 간과 신장부위에 관심영역을 설정하여 간과 심장부위의 시간방사능 곡선을 얻었다. 간기능 평가를 위해 시간방사능 곡선을 이용하여 간섭취지수와 혈중제거지수를 구하였고 곡선 최적화를 시행하였다. DMN 투여군과 대조군의 간효소치의 변화와 간조직의 광학현미경 소견을 비교하였다. 결과: 대조군에서는 ^(99m)Tc-LSA가 빠르게 간에 섭취되고 혈중에서 제거되었으나 DMN을 처리한 군에서는 간섭취가 낮았다. 간섭취지수의 비교에서 대조군에 비해 DMN 처리군에서 유의하게 간섭취지수가 낮았다(DMN-3: 0.842, DMN-8: 0.898, DMN-21: 0.91, 대조군: 0.96, p<0.05). 혈중제거지수의 비교에서도 대조군에 비해 DMN 처리군에서 혈중제거지수가 유의하게 높았다(DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, 대조군: 0.473, p<0.05). 비선형 회귀분석에서 R_(2) 값은 0.9이상으로 좋은 일치를 보였고, 대조군에ㅓ K값이 DMN처리군에 비해 크고(DMN-3: 0.28, DMN-8: 0.41, DMN-21: 0.46, 대조군: 0.97, p<0.05), T_(1/2)값은 작았다(DMN-3: 2.5, DMN-8: 1.7, DMN-21: 1.5, 대조군: 0.7, p<0.05). 간효소치의 변화는 DMN-3군에서는 대조군에 비해 상승하였으나 DMN-8, DMN-21군에서는 간효소치의 상승이 관찰되지 않았다. 간조직 소견의 경우 DMN-3군에서 중심정맥 주위에 괴사가 관찰되었으나 DMN-8군, DMN-21군에서는 미약한 정도의 염증세포 침윤만이 관찰되었다. 결론: ^(99m)Tc-LSA 간신티그래피의 간섭취 정도는 간손상과 반비례하였으며 간섭취의 변화는 조직학적 손상이 심한 정도와 간손상후 회복되는 과정을 반영하여 주었다. ^(99m)Tc-LSA 간신티그래피가 간손상을 평가하고 간손상후 회복되는 과정을 추적하는 간수용체 영상용 방사성 의약품으로 사용될 수 있을 것으로 생각된다. Objects: ^(99m)Tc-lactosylated human serum albumin(LSA) is a newly synthesized radiopharmaceutical that binds to asialoglycoprotein receptors, which are specifically presented on the hepatocyte membrane. Hepatic uptake and blood clearance of LSA were evaluated in rat with acute hepatic injury induced by dimethylnitrosamine(DMN) and results were compared with corresponding findings of liver enzyme profile and these of histologic changes. Materials and Methods: DMN (27 mg/kg) was injected intraperitoneally in Sprague-Dawley rat to induce acute hepatic injury. At 3(DMN-3), 8(DMN-8), and 21(DMN-21) days after injection of DMN, LSA injected intravenously, and dynamic images of the liver and heart were recorded for 30 minutes. Time-activity curves of the heart and liver were generated from regions of interest drawn over liver and heart area. Degree of hepatic uptake and blood clearance of LSA were evaluated with visual interpretation and semiquantitative analysis using parameters (receptor index : LHL3 and index of blood clearance : HH3), analysis of time-activity curve was also performed with curve fitting using Prism program. Results: Visual assessment of LSA images revealed decreased hepatic uptake in DMN treated rat, compared to control group. In semiquantitative analysis, LHL3 was significantly lower in DMN treated rat group than control rat group (DMN-3:0.842, DMN-8: 0.898, DMN-21: 0.91, Control: 0.96, p<0.05), whereas HH3 was significantly higher than control rat group (DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, Control: 0.473, p<0.05). AST and ALT were significantly higher in DMN-3 group than those of control group. Centrilobular necrosis and infiltration of inflammatory cells were most prominent in DMN-3 group, and were decreased over time. Conclusion: The degree of hepatic uptake of LSA was inversely correlated with liver transaminase and degree of histologic liver injury in rat with acute hepatic injury.

경막 내 colistimethate 투여로 치료한 다제 내성 cinetobacter baumannii 뇌실염

홍유아,유진홍,김진진,모은영,안건희,정희경,김진석,이현정,정미향,윤승배 대한감염학회 2009 감염과 화학요법 Vol.41 No.4

Acinetobacter species is a non-fermentative aerobic gram-negative coccobacillus that is an important pathogen found in nosocomial infections. Recently, multi-drug resistant Acinetobacter baumannii (MDR-AB) infections have been increasing and pose a serious problem. Most such infections present as bacteremia, pneumonia, or a wound infection; however, CNS infections are very rare. We herein present a case of ventriculitis caused by MDR-AB in a 37-year old man after a neurosurgical intervention. The patient was successfully treated with intrathecal colistimethate.

스탬핑 성형해석을 위한 유한요소의 자동생성

유동진,윤정환 대진대학교 생산기술연구소 1999 생산기술연구소 논문집 Vol.2 No.-

In the present study, a general method for the mathematical description of arbitrarily shaped surface is proposed by introducing the parametric surface. A polynomial function is employed to descrive the base parametric surface and the boundary curves are defined to describe arbitrary three - dimensional trimmed surfaces. For the mesh generation of initial sheet blank, a grid-based mesh generation algorithm using quadtree is proposed. In order to generate the finite element mesh for tool surface, a hybrid method based on the grid approach and the Delaunay triangulation if proposed. To verify the validity of the proposed method, automatic mesh generation is carried out for some chosen complicated parts including actral automotive panel.

식품 중 보존료의 일제분석에 관한 연구

정진일,민승식,노미정,박유신,남궁석 한국법과학회 2002 한국법과학회지 Vol.3 No.1

As the use of preservatives in foods has been increased, the misuse and overuse of preservatives are occasionally occurred. As the variety of food has risen, it is necessary to establish the screening method to determine preservatives in various foods. In this study, benzoic acid, sorbic acid, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, i-propyl p-hydroxybenzoate, n-butyl p-hydroxybenzoate were analyzed by TLC and HPLC. In order to determine the recoveries from food, these preservatives were spiked into foods. As a result, solvent systems of 8:2:0.45:0.05 and 12:2:0.45:0.05 of pentane:acetic acid:chloroform:ethanol were appropriate for TLC analysis of preservatives. All preservatives were well resolved with solvent system of 2% ammonium acetate in 2.5% acetic acid:methanol (90/10~10/90) by HPLC. Direct extraction of preservatives from food proved to be more proper than distillation and extraction method. The recoveries by direct extraction method showed 93.7~104.6% of preservatives studied.

기도장치 사용후 발생한 기관협착 3례

정진용,유홍균 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.3

Tracheal stenosis can be caused by various etiologies, such as infectious disease, trauma from previous prolonged intubation, airway surgery, or external blunt trauma, and neoplasm. Recently, the development of emergency and intensive care units leads to the primary cause of airway stenosis using airway devices. The stenotic lesions can be produced at any level between the vocal cord and the site of the tip of the tube. Laryngotracheal stenosis may be due to prolonged endotrachel intubation, especially with large tube, large treacheostomy stoma, too highly placed tracheostomy, erosion by local infection, the prying action of heavyweight equipment that connects the tracheostmy to the ventilator, excessive cuff pressure, or erosion by the tip of the tube. Prevention of tracheal stenosis is of key importance by understanding and attending to these causes. We experienced three cases of tracheal stenosis occured after use of airway devices. In first and second cases, We performed end-to-end anastmosis after resection of stenotic segment of the trachea in a 22-year-old female and a 25-year-old male. The had been treated with prolonged endotracheal intubation and emergency tracheostomy, respectively, for a ventilatory support for the respiratory failure after falling down from a height. The stenotic lesions occured at the cuff site in the first case and at the stomal level in the second case. In third case, We performed onestage laryngotracheoplastic procedure for subglottic stenosis in a 23-year-old male. He had been treated with emergency tracheostomy which had been placed too high for a ventilatory support for the respiratory failure after traffic accident. The stenotic lesion occured at the stomal level. The postoperative courses were uneventful.

풍화암에 근입된 영구 앵커의 극한인발력

유남재,박병수,정길수,김진황 江原大學校 産業技術硏究所 2001 産業技術硏究 Vol.21 No.B

The purpose of this study is to estimate ultimate uplift capacity of permanent anchor which was cast into weathered rock. The ultimate uplift capacity was estimated from the load-displacement curve of four different anchors which have different bond length. The creep test was performed for 15minutes under the maximum load of each step in order to understand the load-transfer property of permanent anchor and to decide which anchor to choose. The destruction range of soil due to the changes in load was estimated by installing dial gauge on the ground which was cast into the weathered rock. Ultimately, the study on the behavior of the anchor case into the weathered rock was performed by comparing and analyzing the estimated result of the UUC obtained by the full scale pull out test in the field with the exsting theoretical and practical results of soil and rock anchor.

식품 및 미세증거물 중 함유전분의 종류 판별에 관한 연구

정진일,민승식,어상희,박유신,김재균 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-

일 대학 부속병원 간호사들의 연구장애 요인

유연희,배수정,김진선 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.1

Background and Objectives: Evidence based practice is a hallmark of professional nursing. However, low levels of research utilization are problematic in nursing. The purposes of this study were to identify the facilitators and barriers to research utilization and to identify factors associated with research utilization among nurses. Methods: A descriptive study was conducted with a sample of 227 nurses in a university hospital located in a metropolitan city. The questionnaire included the Barriers Scale and questions about research activities, research related educational needs, and resources used to obtain information for clinical decision making. Results: The main barriers to research utilization identified by the respondents were: the fact that most research is published in a foreign language; implications for practice are not made clear; the research has not been replicated. The greatest mean score of each of the sub-scales was the communication factor followed by the organization, research, and nurse factors. Nurses who had received education for research methods, statistics, and reference search and who had participated research activities reported lower barrier score than those who did not. Conclusions: Although the nurses perceived the importance of research utilization in their practice, there was a poor application in their work. Decreasing known barriers would facilitate research-based practice. The implications for nursing to decrease the barriers to research utilization are discussed.

Acriflavine-Guanosine 복합제 (AG60)의 조직내 분포에 대한 형광현미경적 연구

고정식,임수재,안의태,김진국,김상건,이경영,강종구,홍은경,정영신,유보림,한영복 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.1

해안서식 어패류 중 유해중금속 함량에 관한 연구 (Ⅰ)

유영찬,정진일,박유신,이종숙,인상환,최동기,김동우 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-