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공용해,오동익,김성택 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-
㈜한길은 콘크리트 2차를 가공하는 있는 중소기업이다.이 업체는 현재 재고, 생산, 출고, 매출 관리 등 업무의 전산화가 전혀 이루어져 있지 않기 때문에 업체의 특성상 항상 제품이 적절량을 보유하여야 하는 사항에 있어서는 업무가 비효율적으로 이루어지고 있다.뿐만 아니라 재고, 생산, 출고 관리가 동일한 장소에서만 이루어지는 것이 아니라 지역적으로 분리된 곳에서 이루어지고 있어 정보관리와 공유에 있어서 많은 어려움을 겪고 있다.이에 본 연구에서는 이 업체의 특성에 맞는 효율적인 분산 재고 관리 프로그램의 개발과 지역적으로 분리되어 있는 곳을 근거리 네트워크와 전화접속 네트워크를 혼합한 하이브리드 형태로 구축하여 경제적으로 최적인 재고 관리 시스템을 제공하고, 업체의 생산성 및 경쟁력 향상을 도모하고자 한다.
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임윤규,이두식,박전홍,양기천,김승호,김공식,현관종,김우택,이영순 濟州大學校 農科大學 動物科學硏究所 1993 動物科學論叢 Vol.8 No.1
Enzyme-linked Immuno sorbent Assay (ELISA) for the serological diagnosis oi Bruceiia abortus was developed and compared with plate aggluhnation test Cell wall antigen was extracted from Brucella abortus 1119-3 by sonicabon and with a sodium deoxychlate solution Optimum protein concentra tion of coating antigen 0.4㎍/100㎕ protein on each microtiter plate well. Horse radish peroxidase(HRP) labled protein-G was used as a tracer of reacted antibodies ELISA confirmed the agreeable results of 40 rases out of 43 cases by plate aggulutination test ELISA diagnosed positive cases (10 out of 12) and negatiw cases (1 out of 12) with dubious sera by plate agglutination test From this results EL ISA could be used for the early diagnostic tools of Brucellosis in cattle.
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Metabolism of 14C - Endosulfan in Carp(Cyprinus carpio L .)
Suh, Yong Tack,Shim, Jae Han,Lee, Kong Bong 한국농화학회 1991 Applied Biological Chemistry (Appl Biol Chem) Vol.34 No.4
Fish (Cyprinus carpio L.) were treated with LC_(18) concentration, 4.49 ng/㎖ of ^(14)C endosulfan to study the absorption and metabolism. In an in vivo test, endosulfan was motabolized (65∼80%) in the tissues, only the endosulfan ether was identified indicating that it is the main metabolite of detoxification in carp. However, in an in vitro test endosulfan was converted to the endosulfan α-hydroxyether, endosulfan alcohol and endosulfan ether; the largest quantities were present in tissues where the principal metabolites were endosulfan alcohol and endosulfan ether. The maximum absorption time for tissues (head, muschle, and gut) occured after 8 h exposure. However, the maximum distribution time in liver and kidney was 15 min, 3 h respectively. Radioactivity in the tissue decreased rapidly during the 8 h after treatment. The amount of total recovered ^(14)C-activity in liver, kidney and muscle or fish was about 80∼90% during the 8 h, after application. The non-extractable radioactivity was increased after 8 h exposure (27∼31%). When endosulfan was incubated with the fish liver, kidney and gut preparations, it was metabolized to water solube and organosoluble metabolites. The addition of NADPH resulted in rapid conversion of endosulte to the metabolites in an in vitro test. The rate of metabolism of endosulfan in liver, kidney and gut supplemented with NADPH as a cofactor was higher in the 105,000 g soluble fraction than microsomes.
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박남용,정치영,이창영,기혜영,배성열,이봉주,하용공,윤석민,정병탁,김동성,Park, Nam-yong,Chung, Chi-young,Ri, Chang-yeong,Kee, Hye-young,Bae, Seong-yeol,Lee, Bong-ju,Ha, Yong-kong,Yoon, Seog-min,Jung, Byung-tack,Kim, Dong-sung 대한수의학회 1990 大韓獸醫學會誌 Vol.30 No.4
Encephalomyocarditis virus infection in pigs, characterized by severe reproductive failure in sows and sudden death in less than 7 day-old-piglets, a period of several months from October to December of 1989, in Korea was occurred. The most outstanding gross lesions at necropsy were found in the heart which were consisted of pale, yellow brown necrotic foci of varying size in myocardium. In some cases, pulmonary edema and liver congestion were observed. Histopathology of the heart revealed the interstitial myocarditis, endocarditis and epicarditis with lymphocytic, plasmacytic and macrophage cell infiltration and, in some cases, calcification of the necrotic myocardial muscle fibers. In some cases of brain, the perivascular cuffing and glial nodules were observed. In the liver and the lacrimal gland varying degrees of multifocal necrosis were seen. The virus was isolated from the heart and the brain in a stillborn piglet and a mummified fetus. This outbreak represents the first case of encephalomyocarditis virus infection of pigs in Korea.
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하용공,윤석민,정병탁,박남용,이봉주,정치영,기혜영,배성열,Ha, Yong-kong,Yoon, Seok-min,Jung, Byung-tack,Park, Nam-yong,Lee, Bong-ju,Chung, Chi-young,Kee, Hye-young,Bae, Seong-yeol 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.4
Encephalomyocarditis(EMC) virus was isolated from the mummified and stillborn pigs at a swine farm in Chonnam Province, experienced with EMC infection over the period Oct.~Dec. of 1989. In addition some cultural, serological properties of the isolates and experimental infections in the piglets were studied. The results obtained were as follows; 1. Two EMC virus strains with HA titers and CPE similar to EMC-ATCC were established in a baby hamster kidney (BHK)-21 cell line by inoculating homogenates of brain and heart of the 19 mummified or stillborn pigs and designated $K_3$ and $K_{11}$. 2. At the second BHK-21 cell line passage of the initial isolates CPE appeared after incubation for 16~18 hours, while at the fourth and fifth passage the highest titer of HA was recorded, titer of HA using rat and guinea pig erythrocytes. 3. One pig inoculated with the isolate $K_3$ showed dyspnea as clinical signs and died at the 10 days after inoculation at necropsy white necrotic foci were observed from the dead animal heart. 4. Although all the rest surviving pigs showed increases in antibody titer and body temperature of $40^{\circ}C$ above for the initial 2~4 days followed by the return to normal, there were no gross lesions when the animals were sacrificed at the 2 weeks after inoculation.
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임윤규,이두식,박전홍,양기천,김승호,김공식,현관종,김우택,이영순,Lim, Yoon-kyu,Lee, Doo-sick,Park, Jun-hong,Yang, Ki-chun,Kim, Seung-ho,Kim, Kong-sick,Hyun, Kwan-jong,Kim, Woo-tack,Lee, Yong-soon 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1
Enzyme-linked Immuno sorbent Assay (ELISA) for the serological diagnosis of Brucella abortus was developed and compared with plate agglutination test. Cell wall antigen was extracted from Brucella abortus 1119-3 by sonication and with a sodium deoxychlate solution. Optimum protein concentration of coating antigen were $0.4{\mu}g/100{\mu}{\ell}$ protein on each microtiter plate well. Horse radish peroxidase (HRP) labeled protein-G was used as a tracer of reacted antibodies. ELISA confirmed the agreeable results of 40 cases out of 43 cases by plate aggulutination test. ELISA diagnosed positive cases(10 out of 12) and negative cases (1 out of 12) with dubious sera by plate agglutination test. From this results ELISA could be used for the early diagnostic tools of Brucellosis in cattle.
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