Activation of Transient Receptor Potential Melastatin Family Member 8 (TRPM8) Receptors Induces Proinflammatory Cytokine Expressions in Bronchial Epithelial Cells

김주희,Young Sook Jang,Hwan Il Kim,박지영,박성훈,Yong Il Hwang,Seung Hun Jang,Ki-Suck Jung,Hae Sim Park,Choon-Sik Park 대한천식알레르기학회 2020 Allergy, Asthma & Immunology Research Vol.12 No.4

Purpose: Cold air is a major environmental factor that exacerbates asthma. Transient receptor potential melastatin family member 8 (TRPM8) is a cold-sensing channel expressed in the airway epithelium. However, its role in airway inflammation remains unknown. We investigated the role of TRPM8 in innate immune responses in bronchial epithelial cells and asthmatic subjects. Methods: The TRPM8 mRNA and protein expression on BEAS2B human bronchial epithelial cells was examined by real-time polymerase chain reaction (PCR), immunofluorescence staining and western blotting. Additionally, interleukin (IL)-4, IL-6, IL-8, IL-13, IL-25 and thymic stromal lymphopoietin (TSLP) levels before and after menthol, dexamethasone and N-(4-tert-butylphenyl)-4-(3-chloropyridin-2-yl) piperazine-1-carboxamide (BCTC) treatments were measured via real-time PCR. TRPM8 protein levels in the supernatants of induced sputum from asthmatic subjects and normal control subjects were measured using enzyme-linked immunosorbent assay, and mRNA levels in sputum cell lysates were measured using real-time PCR. Results: Treatment with up to 2 mM menthol dose-dependently increased TRPM8 mRNA and protein in BEAS2B cells compared to untreated cells (P < 0.001) and concomitantly increased IL-25 and TSLP mRNA (P < 0.05), but not IL-33 mRNA. BCTC (10 μM) significantly abolished menthol-induced up-regulation of TRPM8 mRNA and protein and IL-25 and TSLP mRNA (P < 0.01). TRPM8 protein levels were higher in the supernatants of induced sputum from asthmatic subjects (n = 107) than in those from healthy controls (n = 19) (P < 0.001), and IL-25, TSLP and IL-33 mRNA levels were concomitantly increased (P < 0.001). Additionally, TRPM8 mRNA levels correlated strongly with those of IL-25 and TSLP (P < 0.001), and TRPM8 protein levels were significantly higher in bronchodilator-responsive asthmatic subjects than in nonresponders. Conclusions: TRPM8 may be involved in the airway epithelial cell innate immune response and a molecular target for the treatment of asthma.

퇴행성 관절염에서 Interleukin-6와 Soluble Interleukin-6 Receptor

장재석 ( Jae Suk Chang ),정용갑 ( Yong Gab Jeong ),조우신 ( Woo Shin Cho ),빈성일 ( Seong Il Bin ),엄규황 ( Kyu Hwang Ym ),김정화 ( Jung Hwa Kim ) 대한류마티스학회 2000 대한류마티스학회지 Vol.7 No.3

Objective: Unlike other soluble receptors, the soluble interleukin-6 receptor (sIL-6R) cooperates with IL-6 to activate gp130 of effector cell. As the IL-6 and sIL-6R are important in the rheumatoid disease, this study was designed to measure concentration of IL-6 and sIL-6R in synovium and synovial fluid of the degenerative arthritis. Methods: The synovium and synovial fluid were obtained during total knee replacement arthroplasty. The synovium was taken from eleven patients, and synovial fluid taken from sixteen patients. Same patients between two groups were seven. Tissue cultures of the synovial tissues were done with 10% FBS for 72 hours. After irrigation, thery were incubated for 48 hours without FBS, and the culture media and the synovial fluid were collected after centrifuged at 2500rpm for 10 minutes. The level of IL-6 and sIL-6R were measured by quantitative sandwich enzyme immunoassay technique. Results: In the synovium, the IL-6 level was 5.1±0.12ng/ml, and the sIL-6R level was 0.41±0.25ng/ml. In the synovial fluid, the IL-6 level was 0.09± 0.15ng/ml, and the sIL-6R level was 10.37±3.28ng/ml. These results show that IL-6 concentration was measured highly in two groups, especially in synovium (sixty times), and the sIL-6R concentration was measured significantly high in synovial fluid (twenty-five times). Conclusion: The IL-6 and sIL-6R were elevated in degenerative arthrits. We confirmed the source of IL-6 was synovium (very high in synovial tissue culture media), but we need further study for the source of sIL-6R as it was remarkably elevated as IL-6 and its level was lower than serum.

Henoch-$Sch\ddot{o}nlein$ Purpura에서 Interleukin $1{\beta}$ 유전자 다형성과 신장 침범과의 연관성

나형준,고일용,윤준호,예병일,김황민,Na, Hyoung-Joon,Go, Il-Yong,Yoon, Joon-Ho,Yeh, Byung-Il,Kim, Hwang-Min 대한소아신장학회 2006 Childhood kidney diseases Vol.10 No.2

Purpose : High interleukin-1 beta(IL-$1{\beta}$) expression in the skin biopsy specimens of patients with Henoch-$Sch\ddot{o}nlein$ Purpura(HSP) has been observed. We examined IL-$1{\beta}$ gene polymorphism in patients with HSP. The purpose of this study is to examine the relationship between IL-$1{\beta}$ gene polymorphism and renal involvement in HSP. Methods : Patients from mideast Korea with HSP were studied. All patients had at least 6 months of follow up. Patients and ethnically matched controls were genotyped for IL-$1{\beta}$ gene polymorphism by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). Results : Thirty-four patients(all younger than 15 years old) who had been diagnosed with HSP and 27 controls were examined. No allele or genotype differences between the HSP and control groups were observed. No significant association between the carriage of IL-$1{\beta}$(-511) T allele and renal involvement(P=0.525, OR:1.417, CI:0.545-3.686) was found. Conclusion : In unselected patients with HSP, carriage of IL-$1{\beta}$(-511) T allele does not appear to influence renal involvement.

Effects of Chung-Pae Inhalation Therapy on a Mouse Model of Chronic Obstructive Pulmonary Disease

Hwang, Joon-Ho,Lee, Beom-Joon,Jung, Hee Jae,Kim, Kwan-Il,Choi, Jun-Yong,Joo, Myungsoo,Jung, Sung-Ki Hindawi Publishing Corporation 2015 Evidence-based Complementary and Alternative Medic Vol.2015 No.-

<P>Chung-pae (CP) inhalation therapy is a method frequently used in Korea to treat lung disease, especially chronic obstructive pulmonary disease (COPD). This study investigated the effects of CP inhalation on a COPD animal model. C57BL/6 mice received porcine pancreatic elastase (PPE) and lipopolysaccharide (LPS) alternately three times for 3 weeks to induce COPD. Then, CP (5 or 20 mg/kg) was administered every 2 h after the final LPS administration. The effect of CP was evaluated by bronchoalveolar lavage (BAL) fluid analysis, histological analysis of lung tissue, and reverse transcription polymerase chain reaction analysis of mRNA of interleukin- (IL-) 1<I>β</I>, tumor necrosis factor- (TNF-) <I>α</I>, IL-6, and tumor growth factor- (TGF-) <I>β</I>. Intratracheal CP administration reduced the number of leukocytes and neutrophils in BAL fluid, inhibited the histological appearance of lung damage, and decreased the mRNA levels of the proinflammatory cytokines IL-1<I>β</I>, TNF-<I>α</I>, IL-6, and TGF-<I>β</I>. Intratracheal CP administration effectively decreased the chronic inflammation and pathological changes in a PPE- and LPS-induced COPD mouse model. Therefore, we suggest that CP is a promising strategy for COPD.</P>

부산 서부지역의 대기 중 PM_(10) 농도와 화학적 조성 분석

황용식,전병일 한국환경과학회 2003 한국환경과학회지 Vol.12 No.6

The PM_(10) concentration and chemical composition in an western area of Busan were surveyed between March, 2001 and February, 2002. The mean concentration was 98.2 ㎍/m³ with a range of 18.1 to 330.6 ㎍/m³. The magnitude of metallic elements in PM_(10) is as follows in decreasing order: K>Ca>Na>Al>Fe. The mean values of crustal enrichment factors for four elements (Cd, Ni, Pb and Zn) were all higher than 10, which presumably resulted from the effect of anthropogenic origin. Moreover, the wintertime values were higher than springtime and summertime values, possibly due to emissions westerly transported from industries around this area. The contribution of soil particle to airborne particle in the study area was estimated to be 9.5%.

새마을 運動 實績評價模型 作成에 관한 硏究

黃明燦,玄斗日,梁始浩,朴英熙,金龍國,申大淳 건국대학교 1974 學術誌 Vol.17 No.1

The New Village (Sae-ma-eul) movement is Korean version of Community Development that has under-gone widespread exploration, experimentation and application in developing countries throughout the world in recent years. In most countries the national governments have undertaken the iniative in Community Development through appropriate ministries or special agencies. The New Village Movement was initiated in 1970 by Korean government. But it became a people's movement for self-development as more and more people participated in the developmental programs. The major objectives of the New Village movement are to redress the economic disparities between the rural and urban areas by improving the economic, social and cultural conditions of rural Communities, to reform the way of life in rural villages by encouraging the attitude of self-help, and to increase the income of the farmers and fisher man. Since 1971, An enormous amount of public and priate money has been invested into the New Village Movement. It seems that the time has arrived when some framework for evaluating the whole program is to be developed. Thus, The objective of this study is to develop an appropriate to evaluate the new village project at the village level. Ten major factors which are supposed to be relevant for evaluation of the project was selected. The evaluation model thus developed can be summarized in the following format. ◁표 삽입▷ (원문을 참조하세요)

꽃게껍질로 제조된 키토산의 항균효과

황용일,이광현,이승철,하정욱 慶南大學校 附設 工業技術硏究所 1998 硏究論文集 Vol.15 No.3

고분자당류인 chitosan은 가식성 포장제, 항암제, 생물응집제, 항균물질 등으로 폭넓은 이용성을 갖는다. 다량으로 폐기되고 있는 갑각류의 껍질을 효율적으로 이용하게 위하여 꽃게 껍질에서 chitin을 분리정제한 후 탈아세틸화하여 chitosan을 제조하였다. 제조된 chitosan중에서 분자량 42,000이 Escherichia coli O157:NM에 항균력이 가장 우수하였다. 이들 결과로 부터 각종 식중독균에 대하여 항균력을 조사하였다 항균력시험으로 부터 식중독균 중에서 Gram음성균 보다 양성균에 대하여 효과적이 였으며, Gram음성균 중에서도 균종에 따라 상이한 항균력을 보였다. Chitosan was well known as an available material preparing for edible film, antiturmor agent, biocoagulant, and antimicrobiotics etc. For the effective use of Crustacean shell, chitin was prepared from the shell of Blue crab, and chitosan was made through the deacetylation of above chitin. Prepared chitosan with 42,000 M.W. showed most antibacterial effect for Escherichia coli O157 : NM. From this result, chitosan was tested its antibacterial reaction for some Gram prositive and negative food-poisoning bacteria. Antibacterial effect of chitosan was more higher for Gram positive bacteria than Gram negative bacteria and its antibacterial reactions were different for species of Gram negative bacteria.

토끼에서 Etofenprox와 Spinosad 합제의 국소자극성 시험

황윤환,김명석,송인배,박병권,양승호,임용현,송치용,안성한,남현수,임정교,정상일,명노일,윤효인 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2008 動物醫科學硏究誌 Vol.15 No.1

This study was performed to evaluation the skin and eye irritation of etofenprox 8% and spinosad 4% combination in male New Zealand White rabbits. In skin irritation test, the combination of etofenprox and spinosad did not showed any change of body weight but caused slight skin irritation with the P.I.I. (primary irritation index) value 0.25. The etofenprox and spinosad combination showed severe eye irritation 1 h, 24 h, 48 h, and 72 h after ocular treatment, having the A.O.I. (acute ocular irritation) value of 37.3. Therefore, these result indicated that the combination of etofenprox and spinosad might be irritant formulation on eye and skin.

효모의 발현 Vector 계의 개발

황용일,심상국,오시마 야스지,정동효 中央大學校 遺傳工學硏究所 1990 遺傳工學硏究論集 Vol.3 No.1

The cloned gene of glyceraldehyde-3-phosphate dehydrogenase(GAP) of Saccharomyces cerevisiae (Holland et al., 1983) has been characterized. Based on the communication, we have also cloned 2.1kb GAP DNA fragment and modified that fragment as a portable promoter. Two yeast exression vectors, one is a YCp type vector being maintained at low copy number(1 or 2) and the other is a YEp type vector at high copy number, have been constructed with the GAP promoter PH05' gene as an indicator gene. Our plasmids were introduced into Saccharomyces cerevisiae. HU-1, which has been improved. The Trp+ transformants expressed APase activity efficiently and showed the high level of PH05' transcripts.

중학생의 여가만족도가 여가몰입에 미치는 영향

정용각,황두일 부산 외국어 대학교 2003 外大論叢 Vol.26 No.-

This thesis was designed to reveal how the participation in youth group activity affects leisure satisfaction and commitment. The subjects were chosen among middle school students in Pusan using cluster sampling. The total 550 youths were consisted of 300 youths , 25 boys and 25 girls from each six office of education and 250 youths, 50 from each five youth activity group. The researcher visited their schools and the subjects were asked to fill out the distributed questionaries under their consents after enough explanation. The results are as follows. leisure satisfaction by genders does not show remarkable differences in facilities, psychology, rest, sociality satisfaction. Boys show more satisfaction than girls in physicality and interchange satisfaction. Leisure satisfaction by genders does not show remarkable differences in facilities, psychology, rest satisfaction. The attended students in groups showed more satisfaction than non attended students in physicality, interchange, study, and sociality satisfaction. Leisure commitment experience scale by gender does not show differences. The attended students showed higher leisure commitment experience scale than non -attended students in statistics. Statistically, facilities, affiliation, psychology, and study satisfaction have much effect on non-attended students. Generally physicality, facilities, interchange, psychology satisfaction have significant effects on leisure commitment.