Expression of RUNX2/LAPTM5 in the Induction of MC3T3-e1 Mineralization and Its Possible Relationship with Autophagy

Xing Lei,Li Yanqin,Li Wenhao,Liu Rong,Geng Yuanming,Ma Weiqun,Qiao Yu,Li Jianwen,Lv Yingtao,Fang Ying,Xu Pingping 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.6

BACKGROUND: The study aims to correlate osteogenesis with autophagy during the mineralization induction of MC3T3-e1 through exploring the expression of runt-related transcription factor 2 (RUNX2)/lysosomal-associated transmembrane protein 5 (LAMPT5). METHODS: The induction of mineralization in MC3T3-e1 was followed by detecting the expressions of osteogenesisrelated indexes such as RUNX2, alkaline phosphatase (ALP), osteocalcin (OCN), and LAPTM5 using RT-qPCR and Western blot from 0 to 14 days. Transmission electron microscope was utilised in visualizing the alterations of autophagosomes, which was followed by immunofluorescence detecting the subcellular localization of autophagy-related index sequestosome 1 (P62) and microtubule-associated protein 1 light 3 (LC3) protein and scrutinising the expression of P62 mRNA and P62 and LC3 proteins. RESULTS: Induction of MC3T3-e1 mineralization demonstrated an increased expression of osteogenesis-related indicators such as RUNX2, ALP, OCN, and LAPTM5 (p\0.05), as evident from the results of RT-qPCR and Western blot. Meanwhile, the expression of autophagosomes increased one day after mineralization induction and then experienced a gradual decline, and enhanced expression of LC3 protein was noted on days 1–2 of mineralization induction but was then followed by a corresponding reduce. In contrast, a continuous increase was reported in the expression of P62 mRNA and protein, respectively (p\0.05). Up- and down-regulating RUNX2/LAPTM5 expression alone confirmed the aforementioned results. CONCLUSION: It was therefore proposed that RUNX2 may be responsible for an early increase and then a gradual decrease in LAPTM5-mediated autophagy through the regulation of its high expression. Meanwhile, increased LAPTM5 expression in osteogenic mineralization presumed that RUNX2/LAPTM5 promoted autophagy and osteogenic expression, which may play a bridging role in the regulation of autophagy and osteogenesis.

Diversity and Active Mechanism of Fengycin-Type Cyclopeptides from Bacillus subtilis XF-1 Against Plasmodiophora brassicae

( Xing Yu Li ),( Zi Chao Mao ),( Yue Hu Wang ),( Yi Xing Wu ),( Yue Qiu He ),( Chun Lin Long ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.3

Bacillus subtilis XF-1, a strain with demonstrated ability to control clubroot disease caused by Plasmodiophora brassicae, was studied to elucidate its mechanism of antifungal activity against P. brassicae. Fengycin-type cyclopeptides (FTCPs), a well-known class of compounds with strong fungitoxic activity, were purified by acid precipitation, methanol extraction, and chromatographic separation. Eight homologs of fengycin, seven homologs of dehydroxyfengycin, and six unknown FTCPs were characterized with LC/ESI-MS, LC/ESI-MS/MS, and NMR. FTCPs (250μg/ml) were used to treat the resting spores of P. brassicae (107/ml) by detecting leakage of the cytoplasm components and cell destruction. After 12 h treatment, the absorbencies at 260 nm (A260) and at 280 nm (A280) increased gradually to approaching the maximum of absorbance, accompanying the collapse of P. brassicae resting spores, and nearly no complete cells were observed at 24 h treatment. The results suggested that the cells could be cleaved by the FTCPs of B. subtilis XF-1, and the diversity of FTCPs was mainly attributed to a mechanism of clubroot disease biocontrol.

Identification of putative carboxylesterase and aldehyde oxidase genes from the antennae of the rice leaffolder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

Yu-Xing Zhang,Wen-Long Wang,Mao-Ye Li,Shi-Guang Li,Su Liu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.3

In insects, odorant-degrading enzymes (ODEs) play essential roles in the degradation of volatile odorants and maintenance of olfactory sensitivity. ODEs include several enzyme families with detoxification functions, such as carboxylesterases (CXEs) and aldehyde oxidases (AOXs). The rice leaffolder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae), is a serious rice insect pest in Asia. In this study, 18 putative CXE genes and four AOX genes were identified from the antennae of C. medinalis by retrieving a previously released transcriptome dataset. BLASTX searching and phylogenetic analyses showed that these genes are closely related to their respective orthologs in other lepidopteran species. Expression patterns of these genes were determined by reverse transcription- quantitative PCR (RT-qPCR). Four candidate genes, including three CmedCXEs (CmedCXE17, CmedCXE20 and CmedCXE24) and one CmedAOX (CmedAOX2) were antenna-enriched and considered potentially involved in odorant degradation. Our findings provide a comprehensive sequence resource and expression profiles of CXE and AOX genes in C. medinalis antennae, which may facilitate further studies of the odorant degradation mechanisms in this insect species.

Fibulin2: a negative regulator of BMSC osteogenic differentiation in infected bone fracture healing

Li Shi-Dan,Xing Wei,Wang Shao-Chuan,Li You-Bin,Jiang Hao,Zheng Han-Xuan,Li Xiao-Ming,Yang Jing,Guo De-Bin,Xie Xiao-Yu,Jiang Ren-Qing,Fan Chao,Li Lei,Xu Xiang,Fei Jun 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Bone fracture remains a common occurrence, with a population-weighted incidence of approximately 3.21 per 1000. In addition, approximately 2% to 50% of patients with skeletal fractures will develop an infection, one of the causes of disordered bone healing. Dysfunction of bone marrow mesenchymal stem cells (BMSCs) plays a key role in disordered bone repair. However, the specific mechanisms underlying BMSC dysfunction caused by bone infection are largely unknown. In this study, we discovered that Fibulin2 expression was upregulated in infected bone tissues and that BMSCs were the source of infection-induced Fibulin2. Importantly, Fibulin2 knockout accelerated mineralized bone formation during skeletal development and inhibited inflammatory bone resorption. We demonstrated that Fibulin2 suppressed BMSC osteogenic differentiation by binding to Notch2 and inactivating the Notch2 signaling pathway. Moreover, Fibulin2 knockdown restored Notch2 pathway activation and promoted BMSC osteogenesis; these outcomes were abolished by DAPT, a Notch inhibitor. Furthermore, transplanted Fibulin2 knockdown BMSCs displayed better bone repair potential in vivo. Altogether, Fibulin2 is a negative regulator of BMSC osteogenic differentiation that inhibits osteogenesis by inactivating the Notch2 signaling pathway in infected bone.

Staged Improvement in Awareness of Disease for Elderly Cancer Patients in Southern China

Li, Xing,Dong, Min,Wen, Jing-Yun,Wei, Li,Ma, Xiao-Kun,Xing, Yan-Fang,Deng, Yun,Chen, Zhan-Hong,Chen, Jie,Ruan, Dan-Yun,Lin, Ze-Xiao,Wang, Tian-Tian,Wu, Dong-Hao,Liu, Xu,Hu, Hai-Tao,Lin, Jia-Yu,Li, Zhu Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.15

Background: In mainland China, awareness of disease of elderly cancer patients largely relies on the patients' families. We developed a staged procedure to improve their awareness of disease. Materials and Methods: Participants were 224 elderly cancer patients from 9 leading hospitals across Southern China. A questionnaire was given to the oncologists in charge of each patient to evaluate the interaction between family and patients, patient awareness of their disease and participation in medical decision-making. After first cycles of treatment, increased information of disease was given to patients with cooperation of the family. Then patient awareness of their disease and participation in medical decision-making was documented. Results: Among the 224 cancer elderly patients, 26 (11.6%) made decisions by themselves and 125 (55.8%) delegated their rights of decision-making to their family. Subordinate family members tended to play a passive role in decision-making significantly. Patients participating more in medical decision-making tended to know more about their disease. However, in contrast to the awareness of disease, patient awareness of violation of medical recommendations was reversely associated with their participation in medical decision-making. Improvement in awareness of diagnosis, stages and prognosis was achieved in about 20% elderly cancer patients. About 5% participated more actively in medical decision-making. Conclusions: Chinese elderly cancer patient awareness of disease and participation in medical decision-making is limited and relies on their family status. The staged procedure we developed to improve patient awareness of disease proved effective.

The effects of mouse ovarian granulosa cell function and related gene expression by suppressing BMP/Smad signaling pathway

Li Zhang,Hejian Wang,Daolun Yu,Jie Chen,Chaofeng Xing,Jie Li,Jun Li,Yafei Cai 한국통합생물학회 2018 Animal cells and systems Vol.22 No.5

BMP I type receptor inhibitor can selectively inhibit BMP/Smad signaling pathways, mainly by inhibiting the BMP I type receptor activity to prevent phosphorylation of Smad1, Smad5 and Smad9. The aim of the present study was to explore the effects of mouse ovarian granulosa cell function and related gene expression by suppressing BMP/Smad signaling pathway with LDN- 193189(A type of BMP I type receptor inhibitor). In this study, we cultivate the original generation of mouse ovarian granular cells then collect cells and cell culture medium after treatment. Cellular localization and expression of Smad9 and P-smad9 proteins was studied by immunofluorescence (IF) in the ovarian granulosa cells of mouse; Related genes mRNA and proteins expression was checked by QRT-PCR and Western blot; Detected the concentration of related hormones by using ELISA kit; finally, the growth of the cells was analyzed by plotting cell growth curve with CCK-8 assay. The results indicate that, suppression of BMP/Smad signaling pathway can inhibit the expression of LHR and FSHR, inhibit cell proliferation and decrease E2 secretion, the mechanism of action maybe reduce the expression of smad9, at the same time, we found that the feedback regulation of smad9 may affect the expression of FSHR and cell proliferation.

The Correlation between Thyrotropin and Dyslipidemia in a Population-based Study

Li Lu,Beibei Wang,Zhongyan Shan,Fengwei Jiang,Xiaochun Teng,Yanyan Chen,Yaxin Lai,Jiani Wang,Haibo Xue,Sen Wang,Chenyan Li,He Liu,Ningna Li,Jiashu Yu,Liangfeng Shi,Xin Hou,Qian Xing,Xue Bai,Weiping Te 대한의학회 2011 Journal of Korean medical science Vol.26 No.2

This study investigated the relationship between serum thyrotrophin levels and dyslipidemia in subclinical hypothyroid and euthyroid subjects. A total of 110 subjects with subclinical hypothyroidism and 1,240 euthyroid subjects enrolled in this study. Patients with subclinical hypothyroidism had significantly lower high density lipoprotein cholesterol (HDL-C) levels than those who were euthyroid. The lipid profiles were each categorized and mean thyrotrophin levels were higher in subjects in the dyslipidemia subclasses than subjects in the normal subclasses. Thyrotrophin was positively associated with serum triglyceride and negatively associated with serum HDL-C in women. Thyrotrophin was also positively associated with total cholesterol (TC) in the overweight population along with TC and LDL-C in overweight women. In the euthyroid population, thyrotrophin was positively associated with TC in the overweight population. In conclusion, serum thyrotrophin was correlated with dyslipidemia in subclinical hypothyroid and euthyroid subjects; the correlation was independent of insulin sensitivity.

Transcription profile during maternal to zygotic transition in the mouse embryo

Li, Xing-Yu,Cui, Xiang-Shun,Kim, Nam-Hyung CSIRO Publishing 2006 Reproduction, fertility, and development Vol.18 No.6

<P> To gain insight into early embryo development, we used microarray technology to compare gene expression profiles in metaphase II oocytes and one- (1C), two- (2C) and four-cell (4C) embryos. Differences in spot intensities were normalised and grouped using Avadis Prophetic software platform (version 3.3; Strand Genomics, Bangalore, India) and categories were based on the PANTHER and Gene Ontology classification systems. We examined 6927 genes and identified those preferentially expressed in 1C or 2C embryos. We found 1261 genes that were more highly expressed (P < 0.05) in 1C compared with 2C embryos and 1480 genes showing enhanced expression in 2C embryos. Similarly, we investigated 6581 genes present in 2C and 4C embryos and identified 841 that are expressed to a greater extent at the 2C stage and 905 that are more highly expressed at the 4C stage. Using PANTHER classification, genes that were upregulated and downregulated in 2C embryos compared with 1C and 4C embryos were grouped according to their protein functions, which included developmental processes, electron transport, lipid, fatty acid and steroid metabolism, nucleoside, nucleotide and nucleic acid metabolism, protein metabolism and modification, signal transduction and transport, among others. Real time reverse transcription-polymerase chain reaction was performed to confirm differential expression of 14 selected genes. The identification of the genes being expressed will provide insight into the complex gene regulatory networks affecting zygotic genome activation and further development. </P>

Application of Improved Grid Search Algorithm on SVM for Classification of Tumor Gene

Li Wenwen,Xing Xiaoxue,Liu Fu,Zhang Yu 보안공학연구지원센터 2014 International Journal of Multimedia and Ubiquitous Vol.9 No.11

According to the merits and shortcomings of the traditional gridsearch algorithm in parameters optimization of support vector machine (SVM), an improved grid search algorithm is proposed. Dichotomous search algorithm is used to reduce target searching range. First, searching range is determined roughly, and a set of parameters are obtained. Then fine search is applied in reduction the range for searching, and searching the optimum parameters.Three kinds of famous tumor gene data set are used in the comparison experiments to validate the classification accuracy of principal component analysis (PCA)-SVM and kernel principal component analysis (KPCA)-SVM. Experiment results and data analysis shows that, comparing with traditional gridsearch algorithm, the proposed method has higher classification accuracy and less search time.

BRCA1 Gene Exon 11 Mutations in Uighur and Han Women with Early-onset Sporadic Breast Cancer in the Northwest Region of China

Cao, Yu-Wen,Fu, Xin-Ge,Wan, Guo-Xing,Yu, Shi-Ying,Cui, Xiao-Bin,Li, Li,Jiang, Jin-Fang,Zheng, Yu-Qin,Zhang, Wen-Jie,Li, Feng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

The prevalence of BRCA1 gene mutations in breast cancer differs between diverse ethnic groups. Relatively little information is known about patterns of BRCA1 mutations in early-onset breast cancer in women of Uighur or Han descent, the major ethnic populations of the Xinjiang region in China. The aim of this study was to identify BRCA1 mutations in Uighur and Han patients with early-onset (age <35 years), and sporadic breast cancer for genetic predisposition to breast cancer. For detection of BRCA1 mutations, we used a polymerase chain reaction single-stranded conformation polymorphism approach, followed by direct DNA sequencing in 22 Uighur and 13 Han women with early-onset sporadic breast cancer, and 32 women with benign breast diseases. The prevalence of BRCA1 mutations in this population was 22.9% (8/35) among early-onset sporadic breast cancer cases. Of these, 31.8% (7/22) of Uighur patients and 7.69% (1/13) of Han patients were found to have BRCA1 mutations. In 7 Uighur patients with BRCA1 mutations, there were 11 unique sequence alterations in the BRCA1 gene, including 4 clearly disease-associated mutations on exon 11 and 3 variants of uncertain clinical significance on exon 11, meanwhile 4 neutral variants on intron 20 or 2. None of the 11 BRCA1 mutations identified have been previously reported in the Breast Cancer Information Core database. These findings reflect the prevalence of BRCA1 mutations in Uighur women with early-onset and sporadic breast cancer, which will allow for provision of appropriate genetic counseling and treatment for Uighur patients in the Xinjiang region.