A detailed study of physicochemical properties and microstructure of EmimCl-EG deep eutectic solvents: Their influence on SO2 absorption behavior

Jiahong Zhu,Yingjie Xu,Xiao Feng,Xiao Zhu 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.67 No.-

To get a better understanding of the effect of physicochemical properties and microstructure on SO2 absorption behavior of DESs with different molar ratios of EmimCl and EG (from 2:1 to 1:2), densities (ρ), viscosities (η), speeds of sound (u), refractive indices (nD), and thermal decomposition temperatures (Td) of EmimCl-EG DESs were measured and used to obtain the other derived properties, such as thermal expansion coefficient (αp) and activation energy for viscous flow (Eη). Moreover, FT-IR spectra and in situ variable-temperature NMR spectroscopy were employed to study the microstructures of DESs. Based on physicochemical and spectroscopic properties, the influence of the concentrations of EmimCl on the interactions in DESs was explored to be associated with their SO2 absorption behavior. The results show that the interactions between Emim+ and Cl− of EmimCl is gradually weakening with increasing the concentration of EG in DESs by forming of hydrogen bond interaction of OH⋯Cl−, resulting in a decrease of ρ, η, u, nD, and Td of DESs, and hindering the charge–transfer interaction of SO2 with Cl− and deceasing SO2 capture capacity. Moreover, the SO2 absorption capacity of DESs is proportional to their ρ and Eη, respectively.

No Association Between the USP7 Gene Polymorphisms and Colorectal Cancer in the Chinese Han Population

Li, Xin,Wang, Yang,Li, Xing-Wang,Liu, Bao-Cheng,Zhao, Qing-Zhu,Li, Wei-Dong,Chen, Shi-Qing,Huang, Xiao-Ye,Yang, Feng-Ping,Wang, Quan,Wang, Jin-Fen,Xiao, Yan-Zeng,Xu, Yi-Feng,Feng, Guo-Yin,Peng, Zhi-Ha Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Colorectal cancer (CRC), now the third most common cancer across the world, is known to aggregate in families. USP7 is a very important protein with an important role in regulating the p53 pathway, which is critical for genomic stability and tumor suppression. We here genotyped eight SNPs within the USP7 gene and conducted a case-control study in 312 CRC patients and 270 healthy subjects in the Chinese Han population. No significant associations were found for any single SNP and CRC risk. Our data eliminate USP7 as a potential candidate gene towards for CRC in the Han Chinese population.

Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)

Feng-Shan Gao,Xian-zhu Xia,Yu-Wei Gao,Ya-Duo Bai,Xiao-Huan Zou,Gui-Xue Hu 대한수의학회 2009 Journal of Veterinary Science Vol.10 No.3

Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo. The clinical disease profile suggested that the pandas may have suffered a viral infection. Therefore, a series of detection including virus isolation, electron microscopy, cytobiological assay, serum neutralization and RT-PCR were used to identify the virus. It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV. The results suggest that the affected pandas had been infected with CCV.

A Separation Method for Electromagnetic Radiation Sources of the Same Frequency

Xiao, Yingchun,Yang, Yang,Zhu, Feng 한국전자파학회 2023 Journal of Electromagnetic Engineering and Science Vol.23 No.6

To separate electromagnetic interference sources with an unknown source number, a new separation method is proposed, which includes five key steps: spatial spectrum estimation, source number and direction-of-arrival estimation, mixed matrix estimation, separation matrix estimation, and source signal recovery. A pseudospatial spectrum estimation network based on a convolutional neural network is proposed to estimate the number of electromagnetic radiation sources, their direction of arrival, and the mixing matrix. A new loss function is designed as an optimization criterion for estimating the separation matrix. To ensure generalization, both simulated and measured datasets are used to train the proposed network. Experimental results demonstrate that the proposed separation method outperforms existing source separation techniques in terms of correlation coefficient, root mean square error, and running time. Importantly, it exhibits strong performance in underdetermined cases, as well as in overdetermined or determined cases.

Proteomic Analysis of Salt Stress Responses in Rice Shoot

Xiao-Juan Li,Ming-Feng Yang,Yi Zhu,Yu Liang,Shi-Hua Shen 한국식물학회 2011 Journal of Plant Biology Vol.54 No.6

To gain a better understanding of the mechanism of rice (Oryza sativa L.) in response to salt stress, we performed a proteomics analysis of rice in response to 250 mM NaCl treatment using shoots of 3-day-old nascent seedlings. The changes of protein patterns were monitored with two-dimensional gel electrophoresis. Of 57 protein spots showing changes in abundance in response to salt stress, 52 were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The identified proteins were classified into eight functional categories. Several novel salt stress-responsive proteins, including protein synthesis inhibitor I, photosystem II stability/assembly factor HCF136, trigger factor-like protein and cycloartenol-C24-methyltransferase are upregulated upon salt stress. In order to figure out the different and similar molecular mechanism among salt and other stresses,regulation of some salt responsive proteins under other abiotic stress (cold and dehydration) and abscisic acid application was also analyzed. The possible molecular mechanism of rice seedlings in response to salinity and other stresses were discussed.

Genome-wide identification of polyphenol oxidase (PPO) family members in eggplant (Solanum melongena L.) and their expression in response to low temperature

Xiao Kai,Liu Xiaohui,Zhang Aidong,Zha Dingshi,Zhu WeiMin,Tan Feng,Huang Qianru,Zhou Yaru,Zhang Min,Li Jianyong,Wu Xuexia 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.5

Browning of fresh-cut eggplant (Solanum melongena L.) reduces its sensory and nutritional qualities and further influences consumption. Polyphenolic oxidases (PPOs) are key enzymes involved in browning, but the mechanisms that regulate the expression of PPO genes are still unclear. Here, 12 SmPPO genes were identified and phylogenetic analysis clustered these genes into four branches. Protein and cis-regulatory element analyses showed that the SmPPO gene family has a conserved gene structure and diverse functions. Gene expression analysis in different tissues showed that the expression of SmPPO2, SmPPO3, SmPPO6, SmPPO7, and SmPPO10 was higher in the flesh of the browning-sensitive inbred line ‘36’ than in the flesh of the browning-resistant line ‘Fu’. Furthermore, almost all SmPPO genes in ‘36’ were upregulated at 4 °C and 36 °C compared with those in ‘Fu’, and the expression increased earlier after harvest. In addition, SmPPO1, SmPPO6, SmPPO7, and SmPPO10 expression was significantly elevated in ‘36’ after 2 days at 36 °C. These results suggest that SmPPOs are key modulators of eggplant browning and provide candidate genes for further research on the mechanisms regulating fruit browning.

Multilocus Sequence Typing and Virulence Factors Analysis of Escherichia coli O157 Strains in China

Xiao W. Ji,Ya L. Liao,Ye F. Zhu,Hai G. Wang,Ling Gu,Jiang Gu,Chen Dong,Hong L. Ding,Xu H. Mao,Feng C. Zhu,Quan M. Zou 한국미생물학회 2010 The journal of microbiology Vol.48 No.6

Escherichia coli O157:H7, an important food-borne pathogen, has become a major public health concern worldwide. The aim of this study was to investigate the molecular epidemiologic feature of E. coli O157:H7strains in China. 105 E. coli O157:H7 isolates were collected from various hosts and places over 9 years. A multilocus sequence typing scheme (MLST) was applied for bacteria genotyping and polymerase chain reaction (PCR) was used for virulence factor identification. Seven new MLST sequence types (STs), namely ST836, ST837, ST838, ST839, ST840, ST841, and ST842 were identified, which grouped into two lineages. Phylogenetic analysis suggested that the most two frequent STs in China, ST837 and ST836, may be the derivatives of E. coli O157:H7 Sakai or E. coli O157:H7 EDL933. Geographical diversity and host variety of E. coli O157:H7 were observed in China. In addition, the different distribution of tccp was detected. The data presented herein provide new insights into the molecular epidemiologic feature of E. coli O157:H7, and aid in the investigation of the transmission regularity and evolutionary mechanism of E. coli O157:H7.

Taguchi Approach for Anti-heat Stress Prescription Compatibility in Mice Spleen Lymphocytes In Vitro

Xiao-yu Zhu,Gui-lin Cheng,Feng-hua Liu,Jin Yu,Yu-jie Wang,Tong-quan Yu,Jian-qin Xu,Ming Wang 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.7

Heat stress (HS) may induce immunosuppression as well as inhibit the proliferation of lymphocytes. This study evaluated the effects on immune function of our prescription on splenic lymphocytes under HS as well as its compatibility. The effects of four herbal extracts from Agastache rugosa, Atractylodes lancea, Cortex Phellodendri, and Gypsum Fibrosum on heat treated splenic lymphocytes were investigated and the compatibility of the prescription was also explored by using the Taguchi method. This study revealed changes in proliferation by traditional Chinese medicines of splenic lymphocytes after HS. Proliferation in the HS group was significantly lower than the control group. Under HS, the effects of higher concentrations of Agastache rugosa (100 and 200 μg/mL), Atractylodes lancea (100 and 200 μg/mL), Cortex Phellodendri (50 and 100 μg/mL) and Gypsum Fibrosum (100 and 200 μg/mL) caused a significant increase on ConA/LPS-induced proliferation of lymphocytes than lower concentrations. We, therefore, conclude that the prescription of traditional Chinese medicines may recover splenic lymphocytes from the immunosuppression induced by HS. The Taguchi design, which allows rapid and high efficiency for the selection of the best conditions for our prescription on HS-treated splenic lymphocytes, demonstrated that Agastache rugosa (200 μg/mL), Atractylodes lancea (200 μg/mL), Cortex Phellodendri (100 μg/mL) and Gypsum Fibrosum (100 μg/mL)were the optimal conditions for the prescription. The validation experiment confirmed that our composition in optimum extraction conditions enhanced effects on ConA or LPS-stimulated lymphocytes under HS. The results showed that the Taguchi optimization approach is a suitable method for optimization of the composition of prescription.

Impact of a Clinical Pathway on Hospital Costs, Length of Stay and Early Outcomes after Hepatectomy for Hepatocellular Carcinoma

Zhu, Liang,Li, Jun,Li, Xiao-Kang,Feng, Jun-Qiang,Gao, Jian-Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Background: A clinical pathway (CP) can standardize and improve perioperative care for a number of interventions. In hepatic surgery, however, pertinent evidence is very limited. This study was conducted to implement a CP for hepatocellular carcinoma (HCC) patients undergoing hepatectomy, and to evaluate its effects on hospital costs, length of hospital stay (LOHS) and early clinical outcomes. Materials and Methods: Medical records for HCC patients undergoing hepatectomy were retrospectively reviewed before implementation of a CP (the non-CP group) from March 2012 to August 2012. This information was compared with the data collected prospectively from patients after implementation of the CP (the CP group) between September 2012 and April 2013. Hospital costs, LOHS and early clinical outcomes were evaluated and compared between groups. Results: There were no significant differences in terms of patient clinical characteristics between the two groups. For clinical outcome measures, no significant differences were found in postoperative complications, mortality and readmission rate. The hospital costs were significantly reduced from 24,844 RMB in the non-CP group to 19,761 RMB in the CP group (p<0.01). In addition, patients of the CP group also had shorter LOHS compared with the non-CP group (8.3 versus 12.3 days, p<0.001). Conclusions: The CP proved to be an effective approach to minimize hospital costs and LOHS with hepatectomy for HCC without compromising patient care.

Identification of BmELP as an interaction partner of Bmtutl-519 in the silkworm, Bombyx mori

Zhu Feng,Gao Jianhua,Qiao Keyuan,Xiao Shengyan,Bai Xingrong,Kang Meiling,Ming Dongfeng 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

The Turtle protein could act as a signaling receptor or function as a membrane-bound ligand for an unknown receptor. Alternatively, the Turtle protein could function as a co-receptor in a multi-protein receptor complex. Earlier work on the function of Bmtutl-519 suggested that it may act as a cell surface receptor or as a regulatory factor in the infection process of Nosema bombycis. To further investigate the function of Bmtutl-519 in the process of microsporidian infection and the possible regulatory pathways involved, we performed yeast twohybrid screening on a silkworm midgut cDNA library and identified a Bombyx mori extensin-like protein/ BmELP as a binding partner of Bmtutl-519. The interaction between Bmtutl-519 and BmELP was verified by GST pull-down and co-immunoprecipitation assays. Quantitative RT-PCR analysis showed that BmELP was commonly expressed in all of the examined tissues of the silkworm, and the expression was highest in the head. Moreover, BmELP was expressed throughout the entire development period, and the lowest levels of expression were observed at the egg stage. The results of infection experiments showed that N. bombycis infection caused the upregulation of BmELP expression, especially in vitro. Taken together, our findings may provide new insights into the roles of BmELP and Bmtutl-519 in microsporidian infection.