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Gui-Fen Zhou,Li-Qin Li,Jiemiao Lu,Jing Li,Chong Yao,Peng Sun,Kaohua Liu,Yan Dong,Luping Qin,Xiao-Dong Qian 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.3
The quality of commercial saff ron largely depends on apocarotenoid levels. Whether they are aff ected by diff erent fl owercultivation regimes remains unknown. In present study, apocarotenoid levels and the expression profi les of 12 genes relatedto apocarotenoid biosynthesis between fi eld- and indoor-cultivated Crocus sativus L. were analyzed during stigma development. Under both cultivation regimes, apocarotenoid accumulation and gene expression showed similar profi les, fi rstsharply increasing and then gradually decreasing with stigma development, which suggested the coordinated function ofgene expression and apocarotenoid accumulation. However, the rate of increase/decrease varied widely between two blossomregimes at diff erent developmental stages. Based on correlation analysis, βCH1 and ZDS seemed to play a more crucial rolein apocarotenoid accumulation under both cultivation regimes. At anthesis, the total apocarotenoid level in saff ron cultivatedin the fi eld was higher than that cultivated indoors. This may be due to lower gene expression levels in the apocarotenoidpathway, especially βCH1 , ZDS , and CCD2 , and higher CCD4a and NCED expression in the competitive pathway in saff roncultivated indoors than that in the fi eld, which indicated higher conversion in apocarotenoid biosynthesis fl ux from crocinsand picrocrocin synthesis to β-ionone and the ABA biosynthetic branch in saff ron cultivated indoors. These results providevaluable information for modifying planting mode to enhance saff ron quality.
( Xing Long Wang ),( Li Liu ),( Si Xiu Liu ),( Xiao Qing Sun ),( Zhong Xiang Deng ),( Yan Pi ),( Xiao Fen Sun ),( Ke Xuan Tang ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.5
A new CRT binding factor (CBF) gene designated Cbcbf25 was cloned from Capsella bursa pastoris, a wild grass, by the rapid amplification of cDNA ends (RACE). The full-length cDNA of Cbcbf25 was 898 bp with a 669 bp open reading frame (ORF) encoding a putative DRE/CRT (LTRE)-binding protein of 223 amino acids. The predicted CbCBF25 protein contained a potential nuclear localization signal (NLS) in its N-terminal region followed by an AP2 DNA-binding motif and a possible acidic activation domain in the C-terminal region. Bioinformatic analysis revealed that Cbcbf25 has a high level of similarity with other CBF genes like cbfl, cbf2, and cbf3 from Arabidopsis thaliana, and Bncbf5, Bncbf7, Bncbfl6, and Bncbfl7 from Brassica napus. A cold acclimation assay showed that Cbcbf25 was expressed immediately after cold triggering, but this expression was transient, suggesting that it concerns cold acclimation. Our study implies that Cbcbf25 is an analogue of other CBF genes and may participate in cold-response, by for example, controlling the expression of cold-regulated genes or increasing the freezing tolerance of plants.
New Triterpenoids from the Fruits of Schisandra wilsoniana and Their Biological Activities
Gao, Xue-Mei,Li, Yun-Qi,Shu, Li-Dan,Shen, Yan-Qiong,Yang, Li-Ying,Yang, Liu-Meng,Zheng, Yong-Tang,Sun, Han-Dong,Xiao, Wei-Lie,Hu, Qiu-Fen Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.3
Investigation of an organic extract of the fruits Schisandra wilsoniana led to the isolation of two new highly oxygenated nortriterpenoids, named schilancidilactones V-W (1-2). Their structures were elucidated by spectroscopic evidence. Compounds 1-2 feature a double bond between C-7 and C-8 compared with related known nortriterpenoids isolated from the genus Schisandra. Compounds 1 and 2 were tested for their anti-HIV-1 activities and cytotoxicity. The results revealed that compounds 1 and 2 showed moderate anti-HIV-1 activities with $EC_{50}$ 3.05 and 2.87 ${\mu}g/mL$, respectively, and compound 1 showed high cytotoxicity against KB and MDA-MB-231 cell with $IC_{50}$ values of 3.18 and 5.22 ${\mu}M$, respectively.