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Kang Zhi‐Wei,Liu Fang‐Hua,Xu Yong‐Yu,Cheng Jia‐Hui,Lin Xiao‐Li,Jing Xiang‐Feng,Tian Hong‐Gang,Liu Tong‐Xian 한국곤충학회 2021 Entomological Research Vol.51 No.1
Odorant‐degrading enzymes (ODEs) have been found in insect antennae and play a critical role in signal chemical degradation once the message is conveyed. Significant progress has been made in characterizing ODEs in a variety of pests but very little is known in their natural enemies. We have carried out an antennae‐ and sex‐specific transcriptome of Aphidius gifuensis, a natural enemy of aphid, to identify the candidate ODEs. Based on the antennae‐ and sex‐specific transcriptome, a total of 100 putative ODEs were identified including one aldehyde oxidase (AOX), four alcohol dehydrogenases (ADs), eight UDP‐glucuronosyltransferases (UGTs), 45 cytochrome P450 (P450s), nine glutathione S‐transferases (GSTs) and 40 carboxylesterases (CCEs or CXEs). Additionally, we used RT‐qPCR to determine the expression profiles of these genes in tissues of both sexes. Based on the phylogenic analysis and tissue‐expression patterns, AgifEstE4, AgifCXE3, AgifCCE4, AgifCCE7, and AgifCCE18 were suggested as key ODEs in A. gifuensis. In addition, the female or male specifically enriched genes, such as AgifCCE17, AgifEstB1, AgifCYP18a1, AgifUGT2C2, were also considered to involve in the chemosensory processing in A. gifuensis. This study not only identified the candidate ODEs in A. gifuensis but also provided source for further exploration of the molecular mechanisms of chemical signal transductions in A. gifuensis, as well as other hymenopteran species.
Jia, Wei-Juan,Jia, Hai-Xia,Feng, Hui-Yi,Yang, Ya-Ping,Chen, Kai,Su, Feng-Xi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1
Purpose: The purpose of this study was to investigate the recurrence pattern and characteristics of patients based on the 2013 St. Gallen surrogate molecular subtypes after breast-conserving surgery (BCS) in Chinese women. Methods: This retrospective analysis included 709 consecutive breast cancer patients undergoing BCS from 1999-2010 at our institution. Five different surrogate subtypes were created using combined expression of the estrogen receptor, progesterone receptor, and human epidermal growth factor receptor-2. Locoregional relapse-free survival (LRRFS), distant metastasis-free survival (DMFS), and disease-free survival (DFS) rates were calculated. Results: The 5-year LRRFS, DMFS, and DFS rates were 90.5%, 88.2%, and 81.5%, respectively. Multivariate analysis revealed that young age, node-positive disease, and HER2 enrichment were independent prognostic factors in LRRFS patients. There was also an independent prognostic role of lymph node-positive disease in DMFS and DFS patients. Patients with luminal A tumors had the most favorable prognosis, with LRRFS, DMFS, and DFS rates of 93.2%, 91.5%, and 87.4% at 5 years, respectively. Conversely, HER-2-enriched tumors exhibited the highest rate of locoregional recurrence (20.6%). Conclusion: Surrogate subtypes present with significant differences in RFS, DMFS, and LRRFS. Luminal A tumors have the best prognosis, whereas HER2-enriched tumors have the poorest.
Wei Bin,Wang Ya-Kun,Yu Jin-Biao,Wang Si-Jia,Yu Yan-Lei,Xu Xue-Wei,Wang Hong 한국미생물학회 2021 The journal of microbiology Vol.59 No.10
C-Glycosides are an important type of natural product with significant bioactivities, and the C-glycosidic bonds of C-glycosides can be cleaved by several intestinal bacteria, as exemplified by the human faeces-derived puerarin-degrading bacterium Dorea strain PUE. However, glycoside hydrolases in these bacteria, which may be involved in the C-glycosidic bond cleavage of C-glycosides, remain largely unknown. In this study, the genomes of the closest phylogenetic neighbours of five puerarin-degrading intestinal bacteria (including Dorea strain PUE) were retrieved, and the protein-coding genes in the genomes were subjected to sequence similarity network (SSN) analysis. Only four clusters of genes were annotated as glycoside hydrolases and observed in the genome of D. longicatena DSM 13814T (the closest phylogenetic neighbour of Dorea strain PUE); therefore, genes from D. longicatena DSM 13814T belonging to these clusters were selected to overexpress recombinant proteins (CG1, CG2, CG3, and CG4) in Escherichia coli BL21(DE3). In vitro assays indicated that CG4 efficiently cleaved the O-glycosidic bond of daidzin and showed moderate β-D-glucosidase and β-D-xylosidase activity. CG2 showed weak activity in hydrolyzing daidzin and pNP- β-D-fucopyranoside, while CG3 was identified as a highly selective and efficient α-glycosidase. Interestingly, CG3 and CG4 could be selectively inhibited by daidzein, explaining their different performance in kinetic studies. Molecular docking studies predicted the molecular determinants of CG2, CG3, and CG4 in substrate selectivity and inhibition propensity. The present study identified three novel and distinctive glycoside hydrolases, highlighting the potential of SSN in the discovery of novel enzymes from genomic data.
Jia-Wei Wang,Ya Chen,Bai-Zhen Chen 대한금속·재료학회 2014 METALS AND MATERIALS International Vol.20 No.6
δ-MnO2 materials doped with transition-metal cations (Zn, Co, and Ag) were successfully synthesized usinga hydrothermal technique. The structures and morphologies of the obtained oxides were analyzed using X-raydiffraction, scanning electron microscopy and Brunauer-Emmett-Teller measurements. Additionally, the electrochemicalproperties were evaluated through cyclic voltammetry, electrochemical impedance spectroscopyand galvanostatic cycling measurements. The results indicate that the pure and doped samples crystallize inthe δ form with a layered structure and that the Mn/Zn, Mn/Co and Mn/Ag molar ratios are all approximately1:0.09. Both the Zn-doped and pure MnO2 materials exhibit a petal-like morphology; however, the formerhas a higher specific surface area of up to 98.97m2 g1. Furthermore, the Zn-doped MnO2 exhibits a near-rectangularcyclic voltammetry (CV) curve with broad quasi-reversible redox peaks and a specific capacitance of182.9 F g−1 at a CV scan rate of 2mVs1. The Co-doped material exhibits a distinct spiny-fiber morphology, andthe electrochemical performance of this material is significantly worse than that of pure MnO2. The averageattenuation rate of the Ag-doped material is only 0.028% after 1000 cycles, which is lower than that of pureMnO2.
Seismic Factor of Safety for 3D Reinforced Soil Slope with Piles under Unsaturated Condition
Jia-jing Wei,Hong Liao,Li-Li Liu,Jian-qun Zhu 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.9
This study investigates the seismic stability of reinforced slope under a three-dimensional (3D) rotational horn-like failure mechanism, using the pseudo-dynamic approach and provides a new procedure to estimate factor of safety (FS) for slope. Such a procedure is able to figure out the influences of the seismic force, the soil property and the reinforcement by piles. Pseudo-dynamic approach is used to describe the seismic force in the time-to-spatial fields in unsaturated soils where matric suction works. Compared to the pseudo-static approach, the current approach considers the effects of time and spatial through the horizontal and vertical shear waves. For the pile driving, different insertion depths can yield various results, whether two work conditions of considering the pile resistance or not are discussed. FS of this study undergoes a decrement as the increase of the pile resistance and seismic force, indicating that the insertion depth affects FS where FS decreases in the early period of pile driving, after that increases and keeps constant in the late of pile driving, showing evident unimodal characteristics during the pile driving. The new expressions for slope reinforced by piles are presented for practical use in unsaturated soil engineering.