Effect of Water Stress on the Growth and Physiology of Coffee Plants

( Vu,N. T. ),( J. M. Park ),( A. T. Tran ),( T. K. Bui ),( D. C. Vu ),( D. C. Jang ),( I. S. Kim ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2018 강원 농업생명환경연구 Vol.30 No.3

Water stress effects on the growth and physiology of three coffee species, arabica, robusta, and liberica, were investigated. Unlike in plants that had water supply, plant height, leaf length, and leaf width values decreased significantly in arabica and liberica in a water deficit condition but did not statistically differ in robusta coffee plants. The highest values of reduction in growth characteristics of coffee plants under water deficit condition were observed in arabica, but they did not significantly differ from those in robusta or liberica. The highest value of relative water content in a water deficit condition was observed in liberica, but it was similar to that of robusta. The SPAD value and Fv/Fm of all coffee species decreased significantly during the period without irrigation but increased significantly after re-watering. SPAD values of robusta and liberica in a water deficit condition were higher than those of arabica. The highest value of relative ion leakage during drought was observed in arabica. On the other hand, robusta generally appeared to be more vigorous than arabica and liberica, as shown by its relatively lower percentage of wilting plants and higher percentage of recovering plants.

Effect of Grafting Position, Water Content in Substrate on the Survival Rate and Quality of Grafted Tomato Seedlings

박혜선 ( N. T. Vu ),허재윤 ( S. H. Kim ),박영식 ( T. D. Pham ),박성민 ( I. S. Kim ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2015 강원 농업생명환경연구 Vol.27 No.2

The objective of this study was conducted to determine effects of grafting position and water content in the substrate on the survival rate and quality of grafted tomato seedlings. Two grafting positions including above and below the rootstock cotyledons were examined. In addition, three water content levels (mid (control), high and low) in thesubstrate were set up during healing and acclimatization processes. There was no difference in survival rate between two grafting positions, but grafting positions effected on growth of grafted seedlings. The growth parameters and root morphology of seedlings grafted above the rootstock cotyledons were higher than that in seedlings grafted below the rootstock cotyledons, except leaf chlorophyll value and T/R ratio. The maximum survival rate (100%) was observed in mid (control) and high water treatments, but the survival rate (95.3%) decreased in low water treatment. The tallest plant height and largest leaf area were obtained in high water treatment. Leaf chlorophyll content significantly increased in low water treatment. No significant difference was detected among different water treatment with stem diameter, number of leaves. Grafted seedling in low water treatment significantly decreased fresh and dry weight of shoot and root. T/R ratio increased in low water treatment, but it was similar in mid and high water treatments. The high compactness value was observed in mid water treatment. The high values of total root surface area, total root length, and number of root tips were observed in high water treatment. However, there was no significant difference between mid (control) and low water treatments on total root surface area, total root length, and number of toot tips.

Peroxisome proliferator-activated receptor gamma as a theragnostic target for mesenchymal-type glioblastoma patients

Tuyen N. M. Hua,오지웅,김소현,Jayson M. Antonio,Vu T. A. Vo,Jiyeon Om,최종환,Jeong-Yub Kim,Chan-Woong Jung,Myung-Jin Park,정양식 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

Glioblastomas (GBMs) are characterized by four subtypes, proneural (PN), neural, classical, and mesenchymal (MES) GBMs, and they all have distinct activated signaling pathways. Among the subtypes, PN and MES GBMs show mutually exclusive genetic signatures, and the MES phenotype is, in general, believed to be associated with more aggressive features of GBM: tumor recurrence and drug resistance. Therefore, targeting MES GBMs would improve the overall prognosis of patients with fatal tumors. In this study, we propose peroxisome proliferator-activated receptor gamma (PPARγ) as a potential diagnostic and prognostic biomarker as well as therapeutic target for MES GBM; we used multiple approaches to assess PPARγ, including biostatistics analysis and assessment of preclinical studies. First, we found that PPARγ was exclusively expressed in MES glioblastoma stem cells (GSCs), and ligand activation of endogenous PPARγ suppressed cell growth and stemness in MES GSCs. Further in vivo studies involving orthotopic and heterotopic xenograft mouse models confirmed the therapeutic efficacy of targeting PPARγ; compared to control mice, those that received ligand treatment exhibited longer survival as well as decreased tumor burden. Mechanistically, PPARγ activation suppressed proneural–mesenchymal transition (PMT) by inhibiting the STAT3 signaling pathway. Biostatistical analysis using The Cancer Genomics Atlas (TCGA, n=206) and REMBRANDT (n=329) revealed that PPARγ upregulation is linked to poor overall survival and disease-free survival of GBM patients. Analysis was performed on prospective (n=2) and retrospective (n=6) GBM patient tissues, and we finally confirmed that PPARγ expression was distinctly upregulated in MES GBM. Collectively, this study provides insight into PPARγ as a potential therapeutic target for patients with MES GBM.

The reproduction potentials of four entomopathogenic nematode strains related to cost-effective production for biological control

Chau Nguyen N.,Anh Le T.,Vu Nguyen H.,Phuc Hoang K. 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.2

Bioassays to evaluate the mortality, virulence and reproduction potentials of four indigenous EPN strains, SPQ16, S-BM12, H-KT3987 and H-CB3452 on insect larvae of mealworm (Tenebrio molitor) and greater wax moth (Galleria mellonella) revealed the highest mortality rates of two insect larvae at the highest inoculation dose of 100 IJs to range from 89 to 100 percent and 94.3–100 percent at 48 h after inoculation, respectively. Virulence was high for all nematode strains, with LC 50 values between 29.6 and 47.3 IJs/insect host. The highest IJ yields were different between nematode strains and insect host, from 66.8 × 10 3 IJs (S-PQ16) to 118.6 × 10 3 IJs (HKT3987) on T. molitor, and from 54.2 × 10 3 IJs (S-BM12) to 163.3 × 10 3 IJs (H-KT3987) on G. mellonella. The culturing cost in terms of food expenditure for rearing insect larvae varied between insect larvae and nematode strains, from 6.76 to 26.63 USD per billion IJs for nematode strains cultured on T. molitor larvae and from 3.54 to 7.81 USD per billion IJs for nematode strains cultured on G. mellonella larvae. The full cost for a nematode product of 2.5 × 10 9 IJs per hectare, produced through in vivo mass culturing, of the most efficient nematode strain, H-KT3987, was 191.3 USD, slightly cheaper than 199.4 USD for the same nematode product produced through in vitro mass culturing.

Exploration of novel 5'(7')-substituted-2'-oxospiro[1,3]dioxolane-2,3'-indoline-based N-hydroxypropenamides as histone deacetylase inhibitors and antitumor agents

Dung, D.T.M.,Dung, P.T.P.,Oanh, D.T.K.,Vu, T.K.,Hahn, H.,Han, B.W.,Pyo, M.,Kim, Y.G.,Han, S.B.,Nam, N.H. Elsevier 2017 Arabian journal of chemistry Vol.10 No.4

<P>A series of novel 5'(7')-substituted-2'-oxospiro[1,3] dioxolane-2,3'-indoline-based N-hydroxypropenamides were designed, synthesized and evaluated for histone deacetylase (HDAC) inhibition and cytotoxicity. It was found that the compounds in this series displayed potent inhibitory effects against HDAC2 with IC50 values as low as 0.284 mu M, almost comparable to that of SAHA (IC50, 0.265 mu M), a positive control. In Western blot analysis, these compounds also exhibited noted inhibition toward histone deacetylation and this inhibition was found to correlate well with the cytotoxicity of the compounds in three human cancer cell lines. Docking studies indicated the compounds in this series bound to HDAC2 with high binding affinities (similar to -9.8 kcal/mol) compared to SAHA (-7.4 kcal/mol). (C) 2015 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University.</P>

Exploration of some indole-based hydroxamic acids as histone deacetylase inhibitors and antitumor agents

Huong, T. T.,Cuong, L.,Huong, P. T.,Thao, T. P.,Huong, L. T.,Dung, P. T.,Oanh, D. T.,Huong, N. T.,Quan, H. V.,Vu, T. K. Springer Science + Business Media 2017 Chemické zvesti Vol.71 No.9

Effect of Storage Temperature and Duration on Growth and Quality of Tomato Plug Seedlings

( N T Vu ),( S H Kim ),( D C Vu ),( I S Kim ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2015 강원 농업생명환경연구 Vol.27 No.1

The objective of this study was conducted to evaluate effect of storage temperatures (13, 10, 7, 4oC and control (glasshouse condition)) and duration on growth and quality of tomato seedlings. Inhibition of seedling elongation was observed in all low storage temperatures. Plant height and number of leaves of tomato seedlings decreased with decreasing temperature. Leaf chlorophyll value of seedlings in control was lower than that at 13oC temperature, but it was higher than that at 10oC and 7oC temperatures. Chilling injury and curly leaf were observed in seedlings at 10, 7oC temperature after 10 or 20 days in storage, but they were not observed in seedlings at 13oC temperature. The time of expanding leaf and chilling injury index increased with increasing storage duration. Delay flowering time increased with increasing storage duration and decreasing storage temperature. 4oC temperature was not suitable for long-term storage of tomato seedlings. After 10 days in storage, only 37.5% of seedling survival rate was observed at 4oC temperature, but 100% of seedling survival rates were observed at 13oC, 10oC and 7oC temperatures after 10 or 20 days in storage. Although almost growth parameters of tomato seedlings decreased with decreasing temperature after 10 or 20 days in storage, but there was not statistically significant difference between 10 and 7oC temperatures with total root surface area, total root length, and number of root tips. The compactness of tomato seedlings at 13, 10 and 7oC were similar to that in control treatment after 10 days in storage, but it decreased with decreasing storage temperature after 20 days in storage.

Effect of Light-emitting Diode Irradiation during Healing and Acclimatization Period on the Survival Rate and Seedlings Quality of Grafted Pepper

( T. H. Nguyen ),( T. D. Pham ),( H. G. Dong ),( N. T. Vu ),( I. S. Kim ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2014 강원 농업생명환경연구 Vol.26 No.3

The objective of this study was to evaluate influence of light-emitting diode (LED) irradiation during grafting period on the survival rate and quality of grafted pepper seedlings. Irradiation by six light treatments (darkness, white fluorescent lamps (WFL), blue LED, red LED, red+blue LED (1:1), far-red LED) was used to treat grafted pepper seedlings for 10 days during healing and acclimatization period. The maximum (100%) survival rate was recorded in red LED and blue+red LED treatments, but it was not statistically different with the WFL treatment. The lowest survival rate was also observed in the darkness treatment. The growth characteristics of grafted seedlings in red LED were similar with those in blue+red LED treatment, but they were greater than those in darkness, WFL, blue LED, and far-red LED treatments. Stem diameter, leaf number, and leaf area were similar between seedlings treated with WFL, blue LED, red LED, and blue+red LED, but they decreased in seedlings treated with darkness and far-red LED. Fresh and dry weight of shoot and root were similar between seedlings treated with red LED and blue+red LED, but they decreased in seedlings treated with WFL, blue LED, far-red LED, and darkness. The lowest of fresh and dry weights of shoot and root were observed in the darkness treatment. Seedlings treated with red LED, blue+red LED, and WFL were significantly more compact than that in darkness, blue LED, and far-red LED treatments. The root morphology was improved in seedlings treated with red LED by increasing total root surface, total root length, and number of toot tips.

Certainty equivalence adaptive speed controller for permanent magnet synchronous motor

Vu, N.T.T.,Choi, H.H.,Jung, J.W. Pergamon Press 2012 Mechatronics Vol.22 No.6

This paper designs a robust adaptive speed controller based on the certainty equivalence principle for a permanent magnet synchronous motor (PMSM). The proposed controller quickly adapts to the variation of model parameters and load torque, thus it does not need exact knowledge about system parameters. The proposed control system can guarantee good performance such as a fast transient response, a small steady-state error, and a good tracking capability. The proposed control algorithm is executed by using a TMS320F28335 floating point DSP. The simulation and experimental results are given to verify the effectiveness of the proposed method in the presence of the variation of system parameters and load torque.

Measurement of Thermal Neutron Cross-Section and Resonance Integrals of the ⁶⁹(n,γ)⁷⁰Ga and ⁷¹Ga(n,γ)⁷²Ga Reactions at Dalat Research Reactor

P. N. Son,T. T. Anh,C. D. Vu,V. H. Tan 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.59 No.23

Thermal neutron capture cross-sections and resonance integrals for ^(69)Ga and ^(71)Ga were measured by the activation method. The experimental samples with and without a cylindrical Cd-shield case 1 mm in thickness were irradiated in the neutron field of the thermal column facility of Dalat research reactor. The induced activities in the samples were measured by using a high-resolution HPGe detector. Thermal neutron cross-sections for 2200 m/s neutrons and resonance integrals for the ^(69)Ga(n,γ)^(70)Ga and ^(71)Ga(n,γ)^(72)Ga reactions have been obtained relative to the reference values of the ^(197)Au(n,γ)^(198)Au reaction with σ_0 = 98.65 ± 0.09 barn and I_0 = 1550 ± 28 barn. The necessary correction factors for thermal neutron and resonance neutron self-shielding effects were taken into account in the determinations. The present results are discussed and compared with the evaluated data from ENDF/BVII, JEFF 3.0 and JENDL 3.3.