[P3-09] Effect of roasting condition on the chemical composition and oxidative stability of perilla oil

Ting-Ting Zhao,Seung In Hong,In-Hwan Kim 한국식품영양과학회 2010 한국식품영양과학회 학술대회발표집 Vol.2010 No.10

Effects of Gypenosides on Acute Stress in Mice

Ting Ting Zhao,이명구,Keon Sung Shin,최현숙 한국생약학회 2013 Natural Product Sciences Vol.19 No.4

The effects of gypenosides (GPS) on electric footshock (EF)-induced acute stress in mice were investigated. Mice were treated orally with GPS (30 - 400 mg/kg) once a day for 5 days. After 2 days of GPS treatment, mice were exposed to EF stimuli (intensity, 2 mA; interval, 10 s; duration, 3 min) for acute stress for 3 days. Spontaneous locomotor activity was increased by acute EF stress, which was decreased by treatment with GPS (100 and 400 mg/kg). In addition, the increased levels of dopamine and serotonin by acute EF stress in the brain were reduced by treatment with GPS (100 and 400 mg/kg). The serum levels of corticosterone increased by acute EF stress were also reduced by GPS (100 and 400 mg/kg). These results suggest that GPS shows the ameliorating effects on acute EF stress by modulating the activity of dopaminergic and serotonergic neurons, and the serum levels of corticosterone. Clinical trials of GPS need to be conducted further so as to develop promising anti-stress agents.

Effects of (-)-Sesamin on Memory Deficits in MPTP-lesioned Mouse Model of Parkinson's Disease

Ting Ting Zhao,신건성,이명구 한국생약학회 2016 Natural Product Sciences Vol.22 No.4

This study investigated the effects of (-)-sesamin on memory deficits in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-lesioned mouse model of Parkinson's disease (PD). MPTP lesion (30 mg/kg/day, 5 days) in mice showed memory deficits including habit learning memory and spatial memory. However, treatment with (-)-sesamin (25 and 50 mg/kg) for 21 days ameliorated memory deficits in MPTP-lesioned mouse model of PD: (-)-sesamin at both doses improved decreases in the retention latency time of the passive avoidance test and the levels of dopamine, norepinephrine, 3,4-dihydroxyphenylacetic acid, and homovanillic acid, improved the decreased transfer latency time of the elevated plus-maze test, reduced the increased expression of N-methyl-D-aspartate (NMDA) receptor, and increased the reduced phosphorylation of extracellular signal-regulated kinase (ERK1/2) and cyclic AMP-response element binding protein (CREB). These results suggest that (-)-sesamin has protective effects on both habit learning memory and spatial memory deficits via the dopaminergic neurons and NMDA receptor-ERK1/2-CREB system in MPTP-lesioned mouse model of PD, respectively. Therefore, (-)-sesamin may serve as an adjuvant phytonutrient for memory deficits in PD patients.

SNA기법을 활용한 일대일로 항공네트워크 분석 연구 : 중국 화물공항도시를 중심으로

조정정(Zhao, Ting-Ting),윤경준(Yun, Kyong-Jun),진무위(Chen, Mao-Wei),이향숙(Lee, Hyang-Sook) 한국물류학회 2020 물류학회지 Vol.30 No.2

2013년 9월과 10월에 중국 국가주석 시진핑은 ‘일대일로’라는 국가적 전략구축을 대대적으로 제안하였다. 일대일로는 중국을 중심으로 아시아, 유럽, 아프리카 81개국 약 300여개 도시의 교통·물류 인프라를 연결하고자 하는 매우 광범위한 정책이다. 일대 일로 정책을 성공적으로 추진하기 위해 육상과 해상뿐만 아니라 항공까지 포함한 종합적 정책이 필요하나, 항공운송에 대한 고려 는 상대적으로 적은 상황이다. 따라서 본 연구는 소셜네트워크분석(Social Network Analysis, SNA) 기법을 통해 일대일로에 포함되 는 공항도시들에 대한 연결중심성과 매개중심성을 분석하였다. 이를 위해 ICAO(International Civil Aviation Organization; 국제민간 항공기구)의 OFOD(On light Origin and Destination)로부터 2011년과 2016년 항공물동량 자료를 수집하였다. 분석결과, 유라시아 전 체의 항공화물네트워크 밀도는 감소하였으며, 군집화 계수는 약화된 것으로 나타났다. 일대일로에 해당하는 국가의 공항도시 중 ISTANBUL (TURKEY)은 연결중심성와 매개중심성 모두에서 절대적 위치를 차지하고 있으며, SEOUL (REPUBLIC OF KOREA), BANGKOK (THAILAND)과 HONG KONG (CHINA)의 경우 허브 공항도시로서의 역할이 강해지고 있는 것을 알 수 있었다. 중국에서 는 SHANGHAI (CHINA)의 중심성이 가장 높았으며, SHENZHEN (CHINA)가 그 뒤를 이었다. 전반적으로 중국 동남지역은 일대일로 정책하에서 성장을 보인 반면, 동북지역은 상대적으로 더딘 성장을 보이고 있는 것으로 나타났다. 본 연구는 네트워크분석을 이용 한 현황 파악을 토대로 문제점 및 개선방안을 제시하였다. 연구결과는 향후 일대일로 공항들의 경쟁력 강화를 위한 정책 및 전략 수립에 유용한 참고자료로 활용될 수 있을 것이다. In September and October 2013, Chinese President Xi proposed a national strategy of “Belt and Road”. It is a very broad policy to connect transportation and logistics infrastructure in about 300 cities in 81 countries in Asia, Europe and Africa, mainly in China. In order to successfully implement the ‘Belt and Road’strategy, a comprehensive policy including not only land and sea, but also aviation is necessary, but the consideration for air transportation is relatively small. Therefore, this study analyzed the connection center and mediation center of airport cities included in “Belt and Road”through Social Network Analysis (SNA) technique. To this end, air traffic data for 2011 and 2016 was collected from the International Civil Aviation Organization (ICAO) ‘s On Flight Origin and Destination (OFOD). As a result of the analysis, the air cargo network density of Eurasia decreased and the clustering coefficient was weakened. ISTANBUL (TURKEY) among airport cities in “Belt and Road” countries occupies an absolute position in both connection and intermediary, and in the case of SEOUL (REPUBLIC OF KOREA), BANGKOK (THAILAND) and HONG KONG (CHINA) as hub airport cities I could see that the role was getting stronger. In China, SHANGHAI (CHINA) had the highest centrality, followed by SHENZHEN (CHINA). Overall, the southeastern region of China showed growth under a ‘Belt and Road’strategy. while the northeastern region showed relatively slow growth. This study suggested problems and improvement measures based on the current status using network analysis. The research results can be used as a useful reference for establishing policies and strategies to strengthen the competitiveness of airports on a ‘Belt and Road’ basis in the future.

선박에 기인한 대기오염물질 배출량 산정 연구 - 광양항과 울산항을 중심으로

조정정(Zhao, Ting-Ting),윤경준(Yun, Kyong-Jun),이향숙(Lee, Hyang-Sook) 한국항만경제학회 2019 韓國港灣經濟學會誌 Vol.35 No.2

최근 항만에서 기인한 대기오염물질에 대한 심각성이 고조되고 있다. 한국은 무역의존도가 매우 높고 수・출입 교역량의 99.7%가 해상을 통해 운송되고 있어, 항만 대기오염의 정도를 파악하고 이에 대비하기 위한 친환경 정책이 필요하다. 이에 본 연구에서는 많은 물동량을 취급함에도 불구하고 관련 연구가 적었던 광양항과 울산항을 대상으로 선박 접안시 발생하는 대기오염물질 배출량을 산정하였다. 이를 위해 2017년 기준으로 선박의 활동 및 제원자료를 수집하고, 해외 환경기관인 EEA와 EPA 방법론 및 계수를 적용하였다. 그 결과, 광양항은 CO 253.09톤, NOx 1986.61톤, SOx 684.01톤, PM10 47.88톤, PM2.5는 44.69톤, VOC 61.56톤, NH3 0.24톤의 대기오염물질을 배출하였다. 울산항의 경우 CO 212.28톤, NOx 1712.54톤, SOx 573.72톤, PM10 40.16톤, PM2.5는 37.48톤, VOC 51.63톤, NH3 0.20톤을 배출하였다. 이어서 선박 접안시 적용 가능한 친환경 정책인 AMP의 단계적 도입 방안을 제시하였다. 본 연구는 광양항과 울산항을 대상으로 대기오염 정도를 파악한 것으로, 이를 통해 현황을 진단하고, 향후 관련 정책 수립을 위한 참고자료로 활용 가능하다. Recently, air pollution from the marine ports has become a serious issue all over the world. Because marine trade accounts for 99.7% of Korea’s trade, efforts are required to recognize the level of port pollution and establish environmental policies. This study estimates air pollution emitted during the berthing process in the Gwangyang and Ulsan ports. Data on ship activity and characteristics are collected and reasonable methodologies and factors from EEA and EPA are adopted. The results show that 253.09 tons of CO, 1986.61 tons of NOx, 684.01 tons of SOx, 47.88 tons of PM10, and 44.69 tons of PM2.5 are emitted at the Gwangyang port. Further, the Ulsan port emitted 212.28 tons of CO, 1712.54 tons of NOx, 573.72 tons of SOx, 40.16 tons of PM10, and 37.48 tons of PM2.5. A stage-by-stage plan for installing AMP infrastructure is suggested as part of a green port policy. This research provides the current pollution status and contributes guidelines for the direction of future policy.

Whole Cell-mediated Biocatalytic Synthesis of Helicid Cinnamylate and Its Biological Evaluation as a Novel Tyrosinase Inhibitor

Rong-ling Yang,Xi Chen,Yu-ye Song,Qian-lin Zhu,Muhammad Bilal,Yu Wang,Zheng Tong,Ting-ting Wu,Zhao-Yu Wang,Hong-zhen Luo,Xiang-jie Zhao,Ting-ting He 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.3

Tyrosinase inhibitors are clinically effective for treating some dermatological disorders related to melanin hyperpigmentation. Accordingly, the discovery and development of tyrosinase inhibitors have great value in the pharmaceutical and cosmetic industry. Here, a novel tyrosinase inhibitor, 6′-O-cinnamoyl-helicid (helicid cinnamylate) was successfully synthesized by a simple and effective biocatalytic approach with Aspergillus oryzae cells. Investigation of the effects of several key variables on helicid cinnamylate synthesis found that the reaction conversion, reaction rate and regioselectivity reached 99%, 9.40 mM/h and > 99%, respectively, at the optimal conditions with anhydrous acetone as the solvent, whole-cell concentration of 40 mg/mL, and the molar ratio of vinyl cinnamate to helicid of 10 at 45°C. The whole-cells retained 68.87% of its initial activity after reusing for seven batches, indicating a potent application potential in non-aqueous biocatalytic systems. It was worth noting that helicid cinnamylate demonstrated a more potent tyrosinase inhibitory activity with an IC50 value of 3.55 mM than helicid (IC50 = 4.48 mM) and arbutin (IC50 = 5.48 mM), which suggest that helicid cinnamylate could be developed as a more potential tyrosinase inhibitor. In conclusion, this study provides a novel whole-cell catalytic approach for the synthesis of helicid cinnamylate and insight into its application as a tyrosinase inhibitor.

Expression of the Proto-oncogene Pokemon in Colorectal Cancer - Inhibitory Effects of an siRNA

Zhao, Gan-Ting,Yang, Li-Juan,Li, Xi-Xia,Cui, Hui-Lin,Guo, Rui Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Objective: This study aimed to investigate expression of the proto-oncogene POK erythroid myeloid ontogenic factor (Pokemon) in colorectal cancer (CRC), and assess inhibitory effects of a small interference RNA (siRNA) expression vector in SW480 and SW620 cells. Methods: Semi-quantitative reverse transcription-polymerase chain reaction (PCR) and immunohistochemistry were performed to determine mRNA and protein expression levels of Pokemon in CRC tissues. Indirect immunofluorescence staining was applied to investigate the location of Pokemon in SW480 and SW620 cells. The siRNA expression vectors that were constructed to express a short hairpin RNA against Pokemon were transfected to the SW480 and SW620 cells with a liposome. Expression levels of Pokemon mRNA and protein were examined by real-time quantitative-fluorescent PCR and western blot analysis. The effects of Pokemon silencing on proliferation of SW480 and SW620 cells were evaluated with reference to growth curves with MTT assays. Results: The mRNA expression level of Pokemon in tumor tissues ($0.845{\pm}0.344$) was significantly higher than that in adjacent tumor specimens ($0.321{\pm}0.197$). The positive expression ratio of Pokemon protein in CRC (87.0%) was significantly higher than that in the adjacent tissues (19.6%). Strong fluorescence staining of Pokemon protein was observed in the cytoplasm of the SW480 and SW620 cells. The inhibition ratios of Pokemon mRNA and protein in the SW480 cells were 83.1% and 73.5% at 48 and 72 h, respectively, compared with those of the negative control cells with the siRNA. In the SW620 cells, the inhibition ratios of Pokemon mRNA and protein were 76.3% and 68.7% at 48 and 72 h, respectively. MTT showed that Pokemon gene silencing inhibited the proliferation of SW480 and SW620 cells. Conclusion: Overexpression of Pokemon in CRC may have a function in carcinogenesis and progression. siRNA expression vectors could effectively inhibit mRNA and protein expression of Pokemon in SW480 and SW620 cells, thereby reducing malignant cell proliferation.

Gaseous signal molecule SO2 regulates autophagy through PI3K/ AKT pathway inhibits cardiomyocyte apoptosis and improves myocardial fibrosis in rats with type II diabetes

Zhao Junxiong,Wu Qian,Yang Ting,Nie Liangui,Liu Shengquan,Zhou Jia,Chen Jian,Jiang Zhentao,Xiao Ting,Yang Jun,Chu Chun 대한약리학회 2022 The Korean Journal of Physiology & Pharmacology Vol.26 No.6

Myocardial fibrosis is a key link in the occurrence and development of diabetic cardiomyopathy. Its etiology is complex, and the effect of drugs is not good. Cardiomyocyte apoptosis is an important cause of myocardial fibrosis. The purpose of this study was to investigate the effect of gaseous signal molecule sulfur dioxide (SO2) on diabetic myocardial fibrosis and its internal regulatory mechanism. Masson and TUNEL staining, Western-blot, transmission electron microscopy, RT-qPCR, immunofluorescence staining, and flow cytometry were used in the study, and the interstitial collagen deposition, autophagy, apoptosis, and changes in phosphatidylinositol 3-kinase (PI3K)/AKT pathways were evaluated from in vivo and in vitro experiments. The results showed that diabetic myocardial fibrosis was accompanied by cardiomyocyte apoptosis and down-regulation of endogenous SO2-producing enzyme aspartate aminotransferase (AAT)1/2. However, exogenous SO2 donors could up-regulate AAT1/2, reduce apoptosis of cardiomyocytes induced by diabetic rats or high glucose, inhibit phosphorylation of PI3K/AKT protein, up-regulate autophagy, and reduce interstitial collagen deposition. In conclusion, the results of this study suggest that the gaseous signal molecule SO2 can inhibit the PI3K/AKT pathway to promote cytoprotective autophagy and inhibit cardiomyocyte apoptosis to improve myocardial fibrosis in diabetic rats. The results of this study are expected to provide new targets and intervention strategies for the prevention and treatment of diabetic cardiomyopathy.

Effects of different culture systems on the culture of buffalo (Bubalus bubalis) spermatogonia stem cell-like cells in vitro

Ting-Ting Li,Shuang-Shuang Geng,Hui-Yan Xu,Ao-Lin Luo,Peng-Wei Zhao,Huan Yang,Xing-Wei Liang,Yang-Qing Lu,Xiao-Gan Yang,Ke-Huan Lu 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.1

Currently, the systems for culturing buffalo spermatogonial stem cells (SSCs) in vitro are varied, and their effects are still inconclusive. In this study, we compared the effects of culture systems with undefined (foetal bovine serum) and defined (KnockOut Serum Replacement) materials on the in vitro culture of buffalo SSC-like cells. Significantly more DDX4- and UCHL1-positive cells (cultured for 2 days at passage 2) were observed in the defined materials culture system than in the undefined materials system (p < 0.01), and these cells were maintained for a longer period than those in the culture system with undefined materials (10 days vs. 6 days). Furthermore, NANOS2 (p < 0.05), DDX4 (p < 0.01) and UCHL1 (p < 0.05) were expressed at significantly higher levels in the culture system with defined materials than in that with undefined materials. Induction with retinoic acid was used to verify that the cultured cells maintained SSC characteristics, revealing an SCP3+ subset in the cells cultured in the defined materials system. The expression levels of Stra8 (p < 0.05) and Rec8 (p < 0.01) were significantly increased, and the expression levels of ZBTB16 (p < 0.01) and DDX4 (p < 0.05) were significantly decreased. These findings provided a clearer research platform for exploring the mechanism of buffalo SSCs in vitro.

Sleep Duration and Cancer Risk: a Systematic Review and Meta-analysis of Prospective Studies

Zhao, Hao,Yin, Jie-Yun,Yang, Wan-Shui,Qin, Qin,Li, Ting-Ting,Shi, Yun,Deng, Qin,Wei, Sheng,Liu, Li,Wang, Xin,Nie, Shao-Fa Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

To assess the risk of cancers associated with sleep duration using meta-analysis of published cohort studies, we performed a comprehensive search using PubMed, Embase and Web of Science through October 2013. We combined hazard ratios (HRs) from individual studies using meta-analysis approaches. A random effect dose-response analysis was used to evaluate the relationship between sleep duration and cancer risk. Subgroup analyses and sensitivity analyses were also performed. Publication bias was evaluated using Funnel plots and Begg's test. A total of 13 cohorts from 12 studies were included in this meta-analysis, which included 723, 337 participants with 15, 156 reported cancer outcomes during a follow-up period ranging from 7.5 to 22 years. The pooled adjusted HRs were 1.06 (95% CI: 0.92, 1.23; P for heterogeneity =0.003) for short sleep duration, 0.91 (95% CI: 0.78, 1.07; P for heterogeneity <0.0001) for long sleep duration. In subgroup analyses stratified by cancer type, long duration of sleep showed an inverse relation with hormone-related cancer (HR=0.79; 95% CI: 0.65, 0.97; P for heterogeneity =0.009) and a greater risk of colorectal cancer (HR=1.29; 95% CI: 1.09, 1.52; P for heterogeneity =0.346). Further meta-analysis on dose-response relationships showed that the relative risks of cancer were 1.00 (95% CI: 0.99, 1.01; P for linear trend=0.9151) for one hour of sleep increment per day, and 1.00 (95% CI: 0.98, 1.01; P for linear trend=0.7749) for one hour of sleep increment per night. No significant dose-response relationship between sleep duration and cancer was found on non-linearity testing (P=0.5053). Our meta-analysis suggests a positive association between long sleep duration and colorectal cancer, and an inverse association with incidence of hormone related cancers like those in the breast. Studies with larger sample size, longer follow-up times, more cancer types and detailed measure of sleep duration are warranted to confirm these results.