Fine mapping the soybean foxglove aphid resistance gene Raso2 in soybean using 180K Axiom® SoyaSNP genotyping assay

Ju Seok Lee,Sungmin Kim,Sumin Park,Kyungryun Kim,Mijung Cho,Eunsil Kim,Jin Kyo Jung,Jeong-Dong Lee,Jung-Kyung Moon,Namshin Kim,Soon-chun Jeong,Sungtaeg Kang 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Foxglove aphid, Aulacorthum solani (Kaltenbach), is a Hemipteran insect that infected a wide variety of plants worldwide and caused serious yield losses in crops. The foxglove aphid resistance gene, Raso2 was previously mapped from PI 366121 (Glycine soja Sieb. and Zucc.) to a 26cM marker interval on soybean chromosome 7. The development of additional genetic markers, which are mapped closer to Raso2 were required to accurately position the gene to improve the effectiveness of marker assisted selection. The objective of this study was to narrow down the putative QTL region, which is responsible to foxglove aphid resistance in PI366121 using recently developed high-density 180K Axiom SoyaSNP genotyping array. One hundred and forty one F8-derived F12 recombinant inbred lines developed from a cross of susceptible Williams 82 and resistant PI 366121, were used to generate a fine map of Raso2 interval. The phenotyping of antibiosis and antixenosis was done through choice and no-choice assays with total plant damage (TPD) and primary infestation leaf damage (PLD). The composite interval mapping analysis showed that the physical interval between two flanking makers, which was corresponding to Raso2, was narrowed down to 500kb on the Williams 82 genome assembly (Glyma2.0), instead of 4Mb in the previous report using Goldengate assay. In the Raso2 interval, there are about 60 candidate genes, including 4 of NBS-containing putative R genes. This result could be useful in breeding for new foxglove aphid resistant soybean cultivars.

Allele frequency data of 20 STR loci in 2000 Korean individuals

Kim, Sungmin,Park, Hyun-Chul,Kim, Jong-Sik,Nam, Younhyong,Kim, Hye Yeon,Park, Jihye,Chung, Ukhee,Lee, Jinmyung,Lim, Si-Keun,Park, Su-Jeong Elsevier Science B.V., Amsterdam 2017 Forensic science international. Genetics. Suppleme Vol.2017 No.6

Development of Antibiotic Classification for Measuring Antibiotic Usage in Korean Hospitals Using a Modified Delphi Method

Kim Bongyoung,Yoon Young Kyung,Kim Dong-Sook,Jeong Su Jin,안성복,Park Sun Hee,Kwon Ki Tae,Kim Hong Bin,Park Yoon Soo,Kim Shin-Woo,Kiem Sungmin,최준용,The Korean Society of Infectious Diseases,The Korean Soc 대한의학회 2020 Journal of Korean medical science Vol.35 No.30

In 2019, a project designed to develop a system for measuring and comparing antibiotic usage in hospitals was launched in Korea. As part of this project, we developed a means to classify antibiotic usage in Korean hospitals using a modified Delphi method. In results, the following categories of antibiotic classification were accepted for use in Korean hospitals: 1) broad-spectrum antibacterial agents predominantly used for hospital-onset infections in adults, 2) broad-spectrum antibacterial agents predominantly used for community-acquired infections in adults, 3) antibacterial agents predominantly used for resistant gram-positive infections in adults, 4) narrow-spectrum beta-lactam agents in adults, 5) antibacterial agents predominantly used for extensive antibiotic resistant gram-negative bacteria in adults, and 6) total antibacterial agents.

Synchrotron X-ray scattering and photon correlation spectroscopy studies on thin film morphology details and structural changes of an amorphous-crystalline brush diblock copolymer

Kim, Young Yong,Kim, Kyungtae,Jung, Sungmin,Kim, Changsub,Kim, Jehan,Roth, Stephan V.,Sprung, Michael,Vartanyants, Ivan A.,Ree, Moonhor Elsevier 2016 Polymer Vol.105 No.-

<P><B>Abstract</B></P> <P>We investigated structural details and temperature-induced structural changes of an amorphous-crystalline brush diblock copolymer, poly(3-((6-((7-(9H-carbazol-9-yl)heptanoyl)oxy)hexyl)thio)propyl glycidyl ether)<SUB>60</SUB>-<I>b</I>-poly(glycidyl 12-((3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heptadecafluorodecyl)oxy)-12-oxododecanoate)<SUB>20</SUB> (PGK<SUB>60</SUB>-PGF<SUB>20</SUB>) in nanoscale thin films using synchrotron grazing incidence X-ray scattering (GIXS) and X-ray photon correlation spectroscopy (XPCS). Interestingly, the diblock copolymer was found to form a mixture of two different hexagonal cylinder structures (HEX1 and HEX2) where the PGF cylinders were aligned in the film plane. HEX1 was composed of PGF cylinders with higher population of crystals while HEX2 consisted of PGF cylinders with lower population of crystals. Surprisingly, the PGF block chains favorably self-assembled because of strong lateral interactions in the bristles, forming vertical multibilayer structure with partial interdigitation even in the highly confined cylindrical geometry. In heating run up to 340 K, some fraction of HEX2 was transformed to HEX1 via cold crystallization. In contrast, HEX1 was transformed to HEX2 above 340 K because of melting of the PGF crystals. The XPCS analysis found that the HEX structural changes associated with the cold crystallization in the PGF cylinder domains took place with relatively fast dynamics. The HEX structural changes associated with melting of the PGF crystals in the cylinder domains occurred with relatively slow dynamics; this unusual dynamics of structural changes might be attributed to a high energy melting process of PGF crystals against strong lateral interactions of the bristles.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Hierarchical structure of an amorphous-crystalline brush diblock copolymer in nanoscale thin films was investigated. </LI> <LI> The crystallizable block chain makes two different hexagonal cylindrical packing orders. </LI> <LI> The fluoroalkyl containing brush block reveals strong tendency to crystallize even in confined cylindrical geometry. </LI> <LI> The fluoroalkyl containing brush block tends to undergo cold crystallization, causing a fast mode of structural changes. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Nanoscale thin film morphology details and temperature-induced structural changes of an amorphous-crystalline brush diblock copolymer containing carbazole and fluoroalkyl end groups at the bristle ends were investigated using synchrotron X-ray scattering and photon correlation spectroscopy.</P> <P>[DISPLAY OMISSION]</P>

Computer Integrated Surgical Robot System for Spinal Fusion

Kim Sungmin,Chung Goo Bong,Oh Se Min,Yi Byung-Ju,Kim Whee Kuk,Park Jong Il,Kim Young Soo The Korean Society of Medical and Biological Engin 2005 의공학회지 Vol.26 No.5

A new Computer Integrated Surgical Robot system is composed of a surgical robot, a surgical planning system, and an optical tracking system. The system plays roles of an assisting surgeon and taking the place of surgeons for inserting a pedicle screw in spinal fusion. Compared to pure surgical navigation systems as well as conventional methods for spinal fusion, it is able to achieve better accuracy through compensating for the portending movement of the surgical target area. Furthermore, the robot can position and guide needles, drills, and other surgical instruments or conducts drilling/screwing directly. Preoperatively, the desired entry point, orientation, and depth of surgical tools for pedicle screw insertion are determined by the surgical planning system based on CT/MR images. Intra-operatively, position information on surgical instruments and targeted surgical areas is obtained from the navigation system. Two exemplary experiments employing the developed image-guided surgical robot system are conducted.

Fine mapping the UV-B resistance gene in soybean using 180K Axiom SoyaSNP assay

Sungmin Kim,Ju Seok Lee,Sumin Park,Kyungryun Kim,Mijung Cho,Eunsil Kim,Bo-Keun Ha,Sungtaeg Kang 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

The depletion of stratospheric ozone has resulted in increased amount of ultraviolet-B radiation (UV-B: 280-320 nm) reaching the Earth’s surface and could cause significant biological effect in plants. In this study, putative quantitative trait loci (QTL), which is responsible to UV-B resistance in soybean, was identified using recently developed high-density 180K Axiom SoyaSNP genotyping array. A population of 115 recombinant inbred lines (RILs) derived from a cross between susceptible Keunolkong and resistant Iksan 10 was analyzed. A total 8,970 polymorphic SNP markers were used to construct linkage map. The both parents and RILs were grown with supplemental UV-B radiation in a greenhouse condition. Three categories of UV-B induced morphological damage, degree of leaf chlorosis, leaf shape change, and total plant damage were evaluated. Using composite interval mapping analysis, one major QTL associated with all of the phenotypic traits was detected on 7.7cM of soybean chromosome 7 with 22 of LOD score accounting for about 60% of phenotypic variance. Also, the allele from Iksan 10 were responsible for the UV-B resistance. Thus, the UV-B resistance QTL on chromosome 7 from Iksan 10 was designated to qUVBR1, corresponding to 30kb on the Williams 82 genome assembly (Glyma2.0) including 7 candidate genes. This result could be useful in breeding for new foxglove aphid resistant soybean cultivars. In addition, these results provided useful information not only for marker-assisted selection for UV-B resistance soybean, but also for the future identification of putative candidate genes, responsible for UV-B resistance in soybean.

Low-Temperature Fabrication of Amorphous Zinc-Tin-Oxide Thin Film Transistors with In-Situ Annealing Process

Kim, Sungmin,Kim, Ji Min,Heo, Jaeyeong,Kim, Hyeong Joon Electrochemical Society 2017 ECS journal of solid state science and technology Vol.6 No.10

<P>This study analyzes the effect of heating during deposition on the electrical and material properties of zinc tin oxide (ZTO) thin film transistors (TFTs). Instead of post-deposition annealing (PDA) at more than 300°C, the ZTO-TFTs were fabricated by heating the substrate to 150∼200°C with 0∼20% of oxygen in chamber and low temperature PDA in air atmosphere. As a result, it was possible to fabricate devices that show similar electrical characteristics with mobilities of 5.8–27.1 cm<SUP>2</SUP> V<SUP>−1</SUP> s<SUP>−1</SUP>, I<SUB>on</SUB>/I<SUB>off</SUB> of 10<SUP>8</SUP>, and subthreshold swing of 0.15∼1.7 V dec<SUP>−1</SUP>, while lowering the process temperature by more than 100°C. We also proceeded to fabricate a device on polymer substrate, which is the ultimate goal of lowering the process temperature. ZTO-TFTs possessing transparency and flexibility were successfully fabricated on 125 μm polyethylene naphthalate (PEN) substrates. They showed electrical properties with a mobility of 1.7 cm<SUP>2</SUP> V<SUP>−1</SUP> s<SUP>−1</SUP>, I<SUB>on</SUB>/I<SUB>off</SUB> of 10<SUP>8</SUP>, and subthreshold swing of 0.39 V dec<SUP>−1</SUP>.</P>

Hippocampus-dependent cognitive enhancement induced by systemic gintonin administration

Kim, Sungmin,Kim, Min-Soo,Park, Kwanghoon,Kim, Hyeon-Joong,Jung, Seok-Won,Nah, Seung-Yeol,Han, Jung-Soo,Chung, ChiHye The Korean Society of Ginseng 2016 Journal of Ginseng Research Vol.40 No.1

Background: A number of neurological and neurodegenerative diseases share impaired cognition as a common symptom. Therefore, the development of clinically applicable therapies to enhance cognition has yielded significant interest. Previously, we have shown that activation of lysophosphatidic acid receptors (LPARs) via gintonin application potentiates synaptic transmission by the blockade of $K^+$ channels in the mature hippocampus. However, whether gintonin may exert any beneficial impact directly on cognition at the neural circuitry level and the behavioral level has not been investigated. Methods: In the current study, we took advantage of gintonin, a novel LPAR agonist, to investigate the effect of gintonin-mediated LPAR activation on cognitive performances. Hippocampus-dependent fear memory test, synaptic plasticity in the hippocampal brain slices, and quantitative analysis on synaptic plasticity-related proteins were used. Results: Daily oral administration of gintonin for 1 wk significantly improved fear memory retention in the contextual fear-conditioning test in mice.We also found that oral administration of gintonin for 1 wk increased the expression of learning and memory-related proteins such as phosphorylated cyclic adenosine monophosphate-response element binding (CREB) protein and brain-derived neurotrophic factor (BDNF). In addition, prolonged gintonin administration enhanced long-term potentiation in the hippocampus. Conclusion: Our observations suggest that the systemic gintonin administration could successfully improve contextual memory formation at the molecular and synaptic levels as well as the behavioral level. Therefore, oral administration of gintonin may serve as an effective noninvasive, nonsurgical method of enhancing cognitive functions.

Hippocampus-dependent cognitive enhancement induced by systemic gintonin administration

Sungmin Kim,Min-Soo Kim,Kwanghoon Park,Hyeon-Joong Kim,Seok-Won Jung,Seung-Yeol Nah,Jung-Soo Han,ChiHye Chung 고려인삼학회 2016 Journal of Ginseng Research Vol.40 No.1

Background: A number of neurological and neurodegenerative diseases share impaired cognition as a common symptom. Therefore, the development of clinically applicable therapies to enhance cognition has yielded significant interest. Previously, we have shown that activation of lysophosphatidic acid receptors (LPARs) via gintonin application potentiates synaptic transmission by the blockade of Kþ channels in the mature hippocampus. However, whether gintonin may exert any beneficial impact directly on cognition at the neural circuitry level and the behavioral level has not been investigated. Methods: In the current study, we took advantage of gintonin, a novel LPAR agonist, to investigate the effect of gintonin-mediated LPAR activation on cognitive performances. Hippocampus-dependent fear memory test, synaptic plasticity in the hippocampal brain slices, and quantitative analysis on synaptic plasticity-related proteins were used. Results: Daily oral administration of gintonin for 1 wk significantly improved fear memory retention in the contextual fear-conditioning test in mice.We also found that oral administration of gintonin for 1 wk increased the expression of learning and memory-related proteins such as phosphorylated cyclic adenosine monophosphate-response element binding (CREB) protein and brain-derived neurotrophic factor (BDNF). In addition, prolonged gintonin administration enhanced long-term potentiation in the hippocampus. Conclusion: Our observations suggest that the systemic gintonin administration could successfully improve contextual memory formation at the molecular and synaptic levels as well as the behavioral level. Therefore, oral administration of gintonin may serve as an effective noninvasive, nonsurgical method of enhancing cognitive functions.

Charge-Spin Correlation in van der Waals Antiferromagnet NiPS3

Kim, So Yeun,Kim, Tae Yun,Sandilands, Luke J.,Sinn, Soobin,Lee, Min-Cheol,Son, Jaeseok,Lee, Sungmin,Choi, Ki-Young,Kim, Wondong,Park, Byeong-Gyu,Jeon, C.,Kim, Hyeong-Do,Park, Cheol-Hwan,Park, Je-Geun American Physical Society 2018 Physical Review Letters Vol.120 No.13