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In Vivo Measurement of Plant Vitality by the Fluorescence Transient
Soonja Oh,Koh Seok-Chan 한국자원식물학회 2002 한국자원식물학회 학술심포지엄 Vol.2002 No.-
Bottle gourd, Lagenaria siceraria, is one of the oldest cultivated plants. To bigin with, its fruit was used as a complete liquid bottle or container. It was a very widespread cultivated plant in prehistoric times, for example (there) is a report from Peru as early as between 13,000 B.C and 11,000 B.C. The dug-out finds in Japan proved to be about 95,000 years old according to the /sup 14/C analysis. The bottle grourd was the most important plant before the invention of pottery in many areas of Asia, New Guinea, Polynesia, America, and Africa. I would like to suggest that there should be "The Bottle Gourd Age" prior to the Pottery Age. Bottle gourds are also used for various purposes such as food, masks, pipes, musical instruments, medicine, symbols, artistic products and also as penis-sheaths of men's attire. Their purposes number more than 220 including 70 varienties of containers or bottles. I consider that its utilization should be called a culture, as it were, "The gourd culture." The shape and the size of the fruit of bottle gourd have a larger variety than those of any other plant. As for the size, it is reported that they range from those that are shorter than 3cm to those that are ovoid and longer than 60cm in diameter. With regard to the shape of its fruit, the bottle gourd can be classified into 7 groups and even more than 30 races, considering the difference of the size. The seeds are so variable without two horn-like projections, with smooth surface or longitudinalines, white or brown, with smooth corky margin. Generally, it seems that there is no correlation between seem shape and fruit shape. My study shows that the seeds of gourd. My study shows that the seeds of gourd in Asia are so simple in shape and in color except for the size. But the seeds of those in Africa are various and seem to be beyond the confines. Explaining the great diversity of the seeds of those in Africa therefore, they appear to have no correlation among the types of fruit of the
Soonja Oh,William W. Adams III,Barbara Demmig-Adams,Seok Chan Koh 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.1
Korean fi r is an endemic ornamental tree species facing population decline in Korea. To further understand the acclimatoryadjustments it undergoes in response to seasonal extremes, we characterized some of the needle proteins that are upregulatedduring winter. Two-dimensional gel electrophoresis (2-DE), followed by MALDI TOF/TOF MS/MS and Mascot analyses,was used to visualize changes in protein profi les during acclimation to winter stress. From the 2-DE protein profi les ofKorean fi r needles, 226 protein spots were detected, many of which accumulated at higher levels during the winter. Among17 proteins identifi ed, 12 matched proteins associated with photosynthesis and with biotic and abiotic stresses, and eightwere signifi cantly upregulated under winter stress. Upregulated proteins included photosynthetic enzymes sedoheptulose-1,7-bisphosphatase and fructose bisphosphate aldolase of the Calvin–Benson cycle, four proteins related to oxidative stresstolerance, two proteins implicated in biotic defense, one heat-shock protein, and fi ve unknown proteins. However, two otheroxidative-stress-related proteins were present at high levels throughout the year, and a chitinase and the small subunit ofribulose-1,5-bisphosphate carboxylase showed no seasonal adjustments. Thus, Korean fi r needles exhibited winter upregulationof some photosynthetic enzymes, coupled with increased photo protective thermal energy dissipation, and proteins relatedto abiotic and biotic stress resistance. Winter stress, which can include both low temperature and reduced water availability,in the subalpine region of Mount Halla led to an altered physiological equilibrium with increases in key Calvin–Bensoncycle enzymes and increased enzymatic and non-enzymatic protection against oxidative stress.
카드뮴 독성 평가를 위한 은행이끼의 엽록소형광 분석 및 환경지표 선발
오순자 ( Soonja Oh ),고석찬 ( Seok Chan Koh ) 한국환경과학회 2013 한국환경과학회지 Vol.22 No.11
The effects of cadmium ions (Cd2+) on the Chl a fluorescence of Ricciocarpos natans were investigated in order to determine whether Chl fluorescence can be used as a biomarker to estimate the physiological responses of plants to cadmium stress. In all plants treated with Cd2+, the image of Fv/Fm, which represents the maximum photochemical efficiency of PSⅡ, changed as the Cd2+ concentration increased, when treated for 48 h or more. Changes of ΦPSⅡ and QP images were recognized even at 10 μM Cd2+. The Chl a O-J-I-P fluorescence transient was also affected even at 10 μM Cd2+. The fluorescence yield decreased considerably in steps J, I and P in plants treated with Cd2+, although a typical polyphasic rise was observed in non-treated plants. The Chl fluorescence parameters, Fm, Fv/Fo, Sm, SFIabs, PIabs and ETo/CS, decreased as the Cd2+ concentration increased, while the Mo and Kn parameters increased. Peroxidase activity decreased significantly and catalase activity increased as the Cd2+ concentration increased. Because of its sensitivity to Cd2+ Ricciocarpos natans is useful in experiments investigating the responses of plants to cadmium exposure. Several parameters (Fm, Fv/Fo, Sm, SFIabs, PIabs, ETo/CS, Mo and Kn) can be applied to determine quantitatively the physiological states of plants under cadmium stress.