Development of a noninvasive KIM-1-based live-imaging technique in the context of a drug-induced kidney-injury mouse model

Tae-Jun Kwon,Da-Sol Lee,Md. Enamul Haque,Rang-Woon Park,Byungheon Lee,Dongkyu Kim,Yong-Hyun Jeon,Kil-Soo Kim,Sang Kyoon Kim 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

The development of reliable methods to diagnose acute kidney injury is essential to allow the adoption of early therapeutic interventions and evaluate their effectiveness. Based on the fact that kidney injury molecule-1 (KIM-1) expression levels in kidneys are markedly upregulated early after a damage event, here we developed a noninvasive KIM- 1-based molecular imaging technique to detect kidney injury. First, we took advantage of a phage-display platform to select small peptides demonstrating a specific high binding affinity to KIM-1. The promising candidate was conjugated with fluorescent probes, and its imaging potential was validated in vitro and in vivo. This peptide, with the sequence CNRRRA, not only showed a high imaging potential in vitro, allowing a strong detection of KIM-1 expressing cells by microscopy and flow cytometry but also generated a strong kidney-specific signal in live-imaging in vivo experiments in the context of a drug-induced kidney-injury mouse model. Our data overall suggest that the CNRRRA peptide is a promising probe to use in the context of in vivo imaging for the detection of KIM-1 overexpression in damaged kidneys.

Novel Polyhydroxybutyrate-Degrading Activity of the Microbulbifer Genus as Confirmed by Microbulbifer sp. SOL03 from the Marine Environment

( Sol Lee Park ),( Jang Yeon Cho ),( Su Hyun Kim ),( Hong-ju Lee ),( Sang Hyun Kim ),( Min Ju Suh ),( Sion Ham ),( Shashi Kant Bhatia ),( Ranjit Gurav ),( See-hyoung Park ),( Kyungmoon Park ),( Yun-go 한국미생물 · 생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.1

Ever since bioplastics were globally introduced to a wide range of industries, the disposal of used products made with bioplastics has become an issue inseparable from their application. Unlike petroleum-based plastics, bioplastics can be completely decomposed into water and carbon dioxide by microorganisms in a relatively short time, which is an advantage. However, there is little information on the specific degraders and accelerating factors for biodegradation. To elucidate a new strain for biodegrading poly-3-hydroxybutyrate (PHB), we screened out one PHB-degrading bacterium, Microbulbifer sp. SOL03, which is the first reported strain from the Microbulbifer genus to show PHB degradation activity, although Microbulbifer species are known to be complex carbohydrate degraders found in high-salt environments. In this study, we evaluated its biodegradability using solid- and liquid-based methods in addition to examining the changes in physical properties throughout the biodegradation process. Furthermore, we established the optimal conditions for biodegradation with respect to temperature, salt concentration, and additional carbon and nitrogen sources; accordingly, a temperature of 37℃ with the addition of 3% NaCl without additional carbon sources, was determined to be optimal. In summary, we found that Microbulbifer sp. SOL03 showed a PHB degradation yield of almost 97% after 10 days. To the best of our knowledge, this is the first study to investigate the potent bioplastic degradation activity of Microbulbifer sp., and we believe that it can contribute to the development of bioplastics from application to disposal.

Adipic Acid Assisted Sol-Gel Synthesis of Li_1+x(Mn_0.4Ni_0.4Fe_0.2)_1-xO₂ (0 < x < 0.3) as Cathode Materials for Lithium Ion Batteries

Karthikeyan, Kaliyappan,Amaresh, Samuthirapandian,Son, Ju-Nam,Kim, Shin-Ho,Kim, Min-Chul,Kim, Kwang-Jin,Lee, Sol-Nip,Lee, Yun-Sung Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.1

Layered $Li_{1+x}(Mn_{0.4}Ni_{0.4}Fe_{0.2})_{1-x}O_2$ (0 < x < 0.3) solid solutions were synthesized using solgel method with adipic acid as chelating agent. Structural and electrochemical properties of the prepared powders were examined by means of X-ray diffraction, Scanning electron microscopy and galvanostatic charge/discharge cycling. All powders had a phase-pure layered structure with $R\bar{3}m$ space group. The morphological studies confirmed that the size of the particles increased at higher x content. The charge-discharge profiles of the solid solution against lithium using 1 M $LiPF_6$ in EC/DMC as electrolyte revealed that the discharge capacity increases with increasing lithium content at the 3a sites. Among the cells, $Li_{1.2}(Mn_{0.32}Ni_{0.32}Fe_{0.16})O_2$ (x = 0.2)/$Li^+$ exhibits a good electrochemical property with maximum initial capacity of 160 $mAhg^{-1}$ between 2-4.5 V at 0.1 $mAcm^{-2}$ current density and the capacity retention after 25 cycles was 92%. Whereas, the cell fabricated with x = 0.3 sample showed continuous capacity fading due to the formation of spinel like structure during the subsequent cycling. The preparation of solid solutions based on $LiNiO_2-LiFeO_2-Li_2MnO_3$ has improved the properties of its end members.

로스팅 조건에 따른 카카오 닙 첨가 쿠키의 품질 특성 및 항산화 활성의 최적화

김다솔(Da Sol Kim1),오현빈(Hyeonbin Oh),김시연(Si Yeon Kim),이휘림(Phyrim Lee),김영순(Young Soon Kim) 한국조리학회 2020 한국조리학회지 Vol.26 No.3

Humor in the Language Classroom: A Review of the Literature

Kim,Sol,Park,Seon-Ho 한국외국어교육학회 2018 한국외국어교육학회 학술대회 자료집 Vol.2018 No.-

Humor is pervasive in the language classroom. It occurs in the middle of role-plays (Bushnell, 2008), during singing (Cekaite & Aronsson, 2004), in discussions (Pomerantz & Bell, 2011), or when the teacher talks about assignments (Waring, 2013). This study explores the literature on humor in the language classroom and proposes implications for teachers about the use of humor. The features of humor (Cook, 2000) are first presented, and then types of humor in the language classroom are identified. It is commonly accepted that humor functions positively as a safe house, reducing learners’ foreign language learning anxiety. In this safe house, groups can be horizontal and relatively free from supervision (Pratt, 1991). Pomerantz and Bell (2011) insist that jocular talk can provide students with diverse and desirable classroom identities, and it can bring more complicated and creative language use (Canagarajah, 2004; Pratt, 1991). In contrast, humor has its negative functions as well. It can lead to students being distracted. Negative humor may harm others’ feelings and convey negative messages, while being expressed as ‘just joking’ (Bell & Attardo, 2010). It may be used to demean someone or to exclude a certain person. Sometimes, students take subversive identities (Canagarajah, 2004) and reprimand their teachers or make fun of them. Subversive humor acts as a very effective means of challenging the authority in socially acceptable ways (Holmes & Marra, 2002). Many subversive acts were found in Waring (2013). Adult ESL learners were momentarily liberated from classroom rules, pinpointing the teacher’s insufficient knowledge, critiquing the task as being too easy, or demanding that the teacher disclose a personal story. Overall, humor turned out to be helpful in language teaching situations. However, for a better appreciation of humor, language teachers’ help is needed as humor frequently includes cultural connotations. Teachers from some Asian countries are reported to be reluctant to use humor in the class because they are afraid of losing control. For the successful use of humor in the primary school English class in Korea, teachers are advised to provide funny examples, use humorous activities, or teach students interesting cultural information. Based on the findings from the research survey, it is recommended that humor should be lesson-relevant, appropriate to students’ linguistic levels, and used in a careful manner.

Adipic Acid Assisted Sol-Gel Synthesis of Li1+x(Mn0.4Ni0.4Fe0.2)1-xO2 (0 < x < 0.3) as Cathode Materials for Lithium Ion Batteries

Kaliyappan Karthikeyan,Samuthirapandian Amaresh,Ju-Nam Son,Shin-Ho Kim,Min-Chul Kim,김광진,이윤성,Sol-Nip Lee 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.1

Layered Li1+x(Mn0.4Ni0.4Fe0.2)1-xO2 (0 < x < 0.3) solid solutions were synthesized using solgel method with adipic acid as chelating agent. Structural and electrochemical properties of the prepared powders were examined by means of X-ray diffraction, Scanning electron microscopy and galvanostatic charge/discharge cycling. All powders had a phase-pure layered structure with R3m space group. The morphological studies confirmed that the size of the particles increased at higher x content. The charge-discharge profiles of the solid solution against lithium using 1 M LiPF6 in EC/DMC as electrolyte revealed that the discharge capacity increases with increasing lithium content at the 3a sites. Among the cells, Li1.2(Mn0.32Ni0.32Fe0.16)O2 (x = 0.2)/ Li+ exhibits a good electrochemical property with maximum initial capacity of 160 mAhg−1 between 2-4.5 V at 0.1 mAcm−2 current density and the capacity retention after 25 cycles was 92%. Whereas, the cell fabricated with x = 0.3 sample showed continuous capacity fading due to the formation of spinel like structure during the subsequent cycling. The preparation of solid solutions based on LiNiO2-LiFeO2-Li2MnO3 has improved the properties of its end members.

경사진 트레드밀에서 후방보행운동을 통한 넙다리네갈래근 강화와 요통의 관계

박주완,김경,김형욱,김재우,배솔희,서정표,이정구,정하영 대구대학교 특수교육재활과학연구소 2010 再活科學硏究 Vol.28 No.1

목적: 본 연구에서는 경사진 트레드밀에서의 후방 보행 훈련을 통한 넙다리네갈래근의 강화가 요통 환자의 치료에 미치는 영향을 알아보고자 하였다. 대상: 만성 요통을 호소하는 16명의 환자를 대상으로 연구를 실시 하였다. 방법: 트레드밀을 10° 경사로 맞춰놓은 상태에서 대상자들은 하루 20분, 일주일에 3회의 빈도로 2주 동안 훈련을 하였다. 표면 근전도를 가지고 넓다리 곧은근, 안쪽 넓은근, 가쪽 넓은근, 척추 세움근의 %MVIC를 측정하였고, KODI와 VAS를 통해 대상자들의 통증정도를 측정하였다. 결과: 넓다리 곧은근은 훈련전에 비해 훈련을 하고 난 후 상대적으로 높은 근전도를 보였고, 통계적으로 유의하였다(p<.05). 안쪽 넓은근과 가쪽 넓은근 또한 높은 근전도율을 보였지만 통계적으로 유의하지는 않았다. 척추 세움근은 훈련전에 비해 훈련을 하고 난 후 높은 근전도율을 보여주었고, 통계적으로 유의하였다(p<.05). VAS와 KODI의 평균값은 명백하게 감소되었고, 통계적으로 유의하였다(p<.05). 결론: 이상의 결론을 종합하면, 후방보행을 통한 넙다리네갈래근의 강화는 요통의 감소에 효과적이라고 사료되며, 더불어 넙다리네갈래근으로 접근하여 요통을 경감하는 중재방법이 효과적인 치료 방법이 될 수 있다는 것을 의미한다. Purpose: This study was to investigate the influence of strengthening the Quadriceps femoris through backward walking treadmill training in the patients who have lower back pain. Subjects: Sixteen subjects with chronic lower back pain were included. Method: In the training, the treadmill was set to have a inclination of 10 degrees, and the subjects were trained for 20 minutes a day, 3 times a week, for 2 weeks. %MVIC(muscle power) of rectus femoris, vastus medialis oblique, vastus lateralis and erctor spinae were measured with surface EMG, and KODI and VAS were used to measure the subject's level of pain. Results: Quadriceps femoris showed relatively higher average electromyogram rates than that before training(p<.05). Vastus medialis oblique and vastus lateralis also showed higher average electromyogram rates, but no significant change in them was noted. Erector spinae showed higher average electromyogram rates than that before training(p<.05). The average points of VAS and KODI clearly decreased(p<.05). Conclusion: Strengthening Quadriceps femoris seems to be efficient for relieving lower back pain in this study.

석면함유 슬레이트 지붕 물받이 퇴적물 중 석면 섬유 함유율

임지현,한솔민,김현석,신유민,박시은,허정윤,김민영,장봉기 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

This study attempted to determine the degree of asbestos release from the aging slate roof by comparing the asbestos content in the slate roof rain gutter with a colored steel plate (tin plate) over the slate roof. Four slate roof houses located in Haengmok-ri, Asan-si, Chungcheongnam-do, and one house constructed with a colored steel plate on the slate roof were selected to collect the sediment of the roof rain gutter. The asbestos fiber content was calculated by a point counting method using a polarization microscope after pretreatment with conversion treatment and hydrochloric acid treatment. The average asbestos content of the four slate roof rain gutter were 1.89%. However, asbestos was not detected in the Slate covering roof rain gutter, which were constructed on the slate roof. Asbestos fiber content was the highest at 2.89% in the slate roof rain gutter installed in 1976, followed by 2.44% in 1953. From the above results, it is necessary to minimize secondary damage as asbestos fibers released from slate roof houses to the surrounding atmosphere or leaked from slate roofs as rainwater may cause soil pollution and seriously affect residents' health. Although covering with colored steel plates (tin plates) has been shown to prevent the leakage of asbestos fibers to some extent, it is believed that a policy alternative to remove the slate roof as soon as possible is needed to solve the fundamental problem.

Serum clusterin level in children with atopic dermatitis

Sol, In Suk,Kim, Yoon Hee,Lee, Kyung Eun,Hong, Jung Yeon,Kim, Mi Na,Kim, Yun Seon,Oh, Mi Seon,Kim, Min Jung,Yoon, Seo Hee,Park, Young A.,Kim, Kyung Won,Sohn, Myung Hyun,Kim, Kyu-Earn Oceanside Publications 2016 Allergy and asthma proceedings Vol.37 No.4

Genomic and transcriptomic analysis of Korean colorectal cancer patients

Jeon Sol A,Ha Ye Jin,Kim Jong-Hwan,Kim Jeong-Hwan,Kim Seon-Kyu,Kim Yong Sung,Kim Seon-Young,Kim Jin Cheon 한국유전학회 2022 Genes & Genomics Vol.44 No.8

Background: Colorectal cancer (CRC) is the third most common type of diagnosed cancer in the world and has the second-highest mortality rate. Meanwhile, South Korea has the second-highest incidence rate for CRC in the world. Objective: To assess the possible influence of ethnicity on the molecular profile of colorectal cancer, we compared genomic and transcriptomic features of South Korean CRCs with European CRCs. Methods: We assembled a genomic and transcriptomic dataset of South Korean CRC patients (KOCRC; n = 126) from previous studies and European cases (EUCRC; n = 245) selected from The Cancer Genome Atlas (TCGA). Then, we compared the two datasets in terms of clinical data, driver genes, mutational signature, gene sets, consensus molecular subtype, and fusion genes. Results: These two cohorts showed similar profiles in driver mutations but differences in the mutation frequencies of some driver genes (including APC, TP53, PABPC1, FAT4, MUC7, HSPG2, GNAS, DENND5B, and BRAF). Analysis of hallmark pathways using genomic data sets revealed further differences between these populations in the WNT, TP53, and NOTCH signaling pathways. In consensus molecular subtype (CMS) analyses of the study cases, no BRAF mutations were found in the CMS1 subtype of KOCRC, which contrasts with previous findings. Fusion gene analysis identified oncogenic fusion of PTPRK-RSPO3 in a subset of KOCRC patients without APC mutations. Conclusions: This study presents insights into the genomic landscape of KOCRCs and reveals some similarities and differences with EUCRCs at the molecular level.