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( Sangeeta Pandey ),( Rameshwar Tiwari ),( Surender Singh ),( Lata Nain ),( Anil Kumar Saxena ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.8
A total of 10 cellulase-producing bacteria were isolated from soil samples irrigated with paper and pulp mill effluents. The sequencing of 16S rRNA gene revealed that all isolates belonged to different species of genus Bacillus. Among the different isolates, B. subtilis IARI-SP-1 exhibited a high degree of β-1,4-endoglucanase (2.5 IU/ml), β-1,4-exoglucanase (0.8 IU/ml), and β-glucosidase (0.084 IU/ml) activity, followed by B. amyloliquefaciens IARI-SP-2. CMC was found to be the best carbon source for production of endo/exoglucanase and β-glucosidase. The β-1,4-endoglucanase gene was amplified from all isolates and their deduced amino acid sequences belonged to glycosyl hydrolase family 5. Among the domains of different isolates, the catalytic domains exhibited the highest homology of 93.7%, whereas the regions of signal, leader, linker, and carbohydrate-binding domain indicated low homology (73-74%). These variations in sequence homology are significant and could contribute to the structure and function of the enzyme.
Asha Subba,M. Pandey,Sangeeta Jha,Karma Gurmey Dolma ASCONS 2018 INTERNATIONAL JOURNAL OF EMERGING MULTIDISCIPLINAR Vol.2 No.3
The natural spring (dhara) is the main source of drinking water in North Sikkim. Though it is pure water for drinking and other domestic purposes, it gets polluted with various environmental factors. The study was performed to assess the quality of spring water. A total of nine sampling sites were selected at an interval of four months, i.e. monsoon, pre-monsoon and post-monsoon during August 2015-July2016. All the spring water samples were analyzed in terms of physical properties, heavy metals like copper, lead, iron, chromium, cadmium and bacterial characteristics such as Escherichia coli, Klebsiella sp, Salmonella sp, Shigella sp, Proteus sp, Citrobacter sp, Enterobacterclocae etc. In the study all the observed results were compared with Bureau of Indian Standard. All the results of physical parameters and heavy metals were found within the permissible limit except the range of pH and iron shows slightly higher concentration than permissible limit. During monsoon, isolation of organisms was recorded in significant percentage followed by post-monsoon and pre-monsoon.