용인지역 노인의 식습관 및 영양섭취 실태조사

이경희,박혜련 명지대학교 대학원 1997 대학원논문집 Vol.1 No.-

This study was performed to assess the health and nutritional status of 125 elderly living in the Yongin area, Kyonggi-do. Survey questionnaires including 24-hr dietary recall, food habit, anthropometric measurements were applied as the instruments. The average age of the subjects were 77 for male and 75 for female. Mean value of BMI was 23.2. About 30% of the subjects was overweight, which is over 25 of BMI. Caloric intake and protein intake were about 50% of the Korean RDA. Their diets were inappropriate, showing C: P: F ratio = 75: 15: 10. Female elderly showed higher food habit score than male elderly(p<0.05).

표고버섯 렉틴의 사이토카인 생성 양상

이인경,김희선,전경희,김성광,정시련 영남대학교 약품개발연구소 1995 영남대학교 약품개발연구소 연구업적집 Vol.5 No.-

The gene expressions of 5 cytokines(IL-1, IL-2, IL-6, TNFα and IFNy) from human peripheral blood mononuclear cells(PBMC) stimulated with Lentinus edodes lectin(LEL) were investigated by using reverse transcription polymerase chain reaction(RT-PCR). PBMC(1×10^(6) cells/ml) isolated from healthy volunteers were stimulated with LEL(O.D.=3.0) or lipopolysaccharide(LPS, 10㎎/ml) for various times(1, 8, 24, 48, 72, 96 and 120 hours). After each of the various stimulated times, total RNA was isolated by RNA sol B method and assessed for various cytokines mRNA by RT-PCR. The mRNA encoding IL-6 and IFNy were detected continuously from 1 to 120 hours. The mRNA encoding IL-1 were detected up to 72 hours and the intensity of the band were increased from 24 to 72 hours. The mRNA encoding IL-2 were detected from 8 to 120 hours and showed delayed reaction compare with other cytokines, But the mRNA encoding TNFα was detected up to 24 hours only. The patterns of TNfα gene expression showed short time response compared with other cytokines. These results suggest that LEL as an inducers of cytokines, can elicits a detectable cytokine on mRNA from the PBMC within the first few hours of stimulation.

호산구성 간농양 54예의 임상상

강혜련,권혁수,송우정,이상민,김태범,박혜경,장윤석,김윤근,조상헌,민경업,김유영 대한알레르기학회 2004 천식 및 알레르기 Vol.24 No.2

Rhizobium 렉틴의 분리ㆍ정제 및 특성 연구

전경희,정시련,이인경,고성진 嶺南大學校 基礎科學硏究所 1993 基礎科學硏究 Vol.13 No.-

Table 1. Specificity of Rhizobium sp. crude lectin in agglutinating erythrocytes from various animals (그림) [HU]: hemagglutinatin unit Table 2. Specificity of intracellular crude lectin from Rhizobium sp. in agglutinating of erythrocytes from various animals (그림) [HU]: hemagglutinatin unit Table 3. Hemagglutination of intracellular crude lectin from Rhizobium sp. in culture age (그림) ※[HU]: hemagglutinatin unit (그래프) Fig.6 Stability of Rhizobium japonicum iectin activity at different pH. Rhizobium japonicum crude lectin was incubated in buffers at different pH(2.18-10.80) for 24hr at 4℃ and the remaining activity was determined. Table 4. Effect of mental ions and chelating agent in hemagglutinating activity for Rhizobium sp. crude lectin (그림) *Chelating agent (그래프) Flg.7 Stability of Rhizobium japonicum iectin activity at different temperature. Rhizobium japonicum was incubated for 30min. After immediate cooling in an ice-water bath, the remaining activity was determined. 조사한 결과 3일째 부터 약한 활성을 나타냈으며, 6일째에 32HU로 최대의 활성을 나타내었다. 배지로 분비된 렉틴은 사람 적혈구와 토끼 적혈구에서 응집현상을 나타내었으며 trypsin처리후 토끼와 DEAE 0.2M 분획에서 하나의 major band와 minor band가 나타났으며, Rhizobium sp. 렉틴의 활성은 pH 7-9범위에서 안정한 것으로 나타났으며, 또한 온도의 영향은 50℃이하에서 안정하였다. 적혈구 응집 저해 효과는 25mM galactose에서 최대로 나타났다. 세포내 결합형 렉틴을 배양시기(1, 2, 4, 6일)에 따라 전기 영동 시험을 실시한 결과 동일한 band pattern과 렉틴 활성을 나타냈으므로, 세포내 결합형 렉틴은 배양 시기 별로 차이가 없음을 알 수 있었다. Table 5. The comparison of protein and carbohydrate contents in Rhizobium sp. crude lectin (그림) a : YMA broth media b : Cell was ruptured by sonication. Crude lectins were partially purified from Rhizobium sp. by salt fractionation and ion exchange chromatography on DEAE-Sephadex A-50. Hemagglutination of extracts from culture of Rhizobium sp. with age showed remarkable activity in 6 days. With purified lectin several biochemical properties have been characterized: Rhizobium sp. lectin agglutinated nonspecifically erythrocytes of human and several animals. In SDS-polyacrylamide gel electrophoresis, Rhizobium sp. lectin (0.2M fraction of DEAE Sephadex A-50) showed one major and one minor bands. Crude lectin from Rhizobium sp. was relatively stable at the range fo pH 7-9 and temperature below 50℃. In sugar inhibition test, this lectin was inhibited by only galactose at final concentration of 25mM. Meanwhile, intracellular crude lectin from Rhizobium sp. with culture age showed same band patterns by polyacrylamide gel electrophoresis.

자동차 배기시스템의 배압특성에 관한 실험적연구

신동륜,이준서,차경옥 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

Based on Experimental analysis, the characteristics of back pressure in automotive exhaust system is tested for 4-stroke gasoline engine. The back pressure in automotive exhaust system is generated by resistance working of exhaust system, i.e. exhaust manifold, pipe length, pipe banding, difference system pressure with atmospheric pressure. This paper contains experimental results which are tested for the change of exhaust pipe length and torque change are tested under experimental conditions.

감염 조절용 차단막의 두께가 광중합기의 중합광에 미치는 영향

장훈상,이석련,홍성옥,류현욱,송창규,민경산 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.5

연구목적: 본 연구는 감염 조절용 차단막을 여러 겹으로 사용했을 때 광중합기의 광강도와 파장, light diffusion 등에 미치는 영향에 대해 조사하였다. 연구 재료 및 방법: 감염 조절용 차단막은 투명 랩 (크린랩)을 사용하였고 광중합기는 할로겐 광중합기 (Optilux 360)와 LED 광중합기 (Elipar FreeLight 2)를 사용하였다. 차단막을 1겹, 2겹, 4겹, 8겹으로 광중합기의 광섬유말단을 감싸고 휴대용 광강도 측정기 (Cure Rite)로 광중합기의 광강도를 측정하였다. 광중합기를 주문제작한 optical breadboard에 고정시킨 후 휴대용 spectroradiometer (CS-1000)를 이용하여 광중합기의 파장을 측정하였고, DSLR (Nikon D70s)을 이용하여 광중합기의 light diffusion을 사진 촬영하였다. 결과: 광강도 측정 결과는 차단막의 두께가 증가할수록 광강도가 유의하게 감소하였으나 할로겐 광중합기에서 1겹과 2겹 사이에는 유의차가 없었으며, 4겹 이상의 차단막을 투과할 때 광강도가 더 많이 감소하였다. 여러 겹의 차단막을 투과한 광중합기의 전체적인 파장 형태와 peak wavelength의 변화는 관찰되지 않았다. Light diffusion 사진 촬영 시, LED 광중합기에서는 차단막의 두께가 미치는 영향이 없었으나 할로겐 광중합기에서는 차단막을 4겹 사용했을 때부터 중합광이 조사되는 각도가 감소하기 시작하여 8겹 사용했을 때 통계적으로 유의하게 감소하는 것을 볼 수 있었다 (p < 0.05). 결론: 광중합형 복합레진을 광중합할 경우 감염 조절용 차단막이 찢어지는 경우를 대비하여 1겹으로 사용하기 보다는 2겹으로 사용하는 것이 환자간의 교차감염을 예방하는데 유리할 것으로 사료된다. Objectives: This study investigated the effect of infection control barrier thickness on power density, wavelength, and light diffusion of light curing units. Materials and Methods: Infection control barrier (Cleanwrap) in one-fold, two-fold, four-fold, and eightfold, and a halogen light curing unit (Optilux 360) and a light emitting diode (LED) light curing unit (Elipar FreeLight 2) were used in this study. Power density of light curing units with infection control barriers covering the fiberoptic bundle was measured with a hand held dental radiometer (Cure Rite). Wavelength of light curing units fixed on a custom made optical breadboard was measured with a portable spectroradiometer (CS-1000). Light diffusion of light curing units was photographed with DSLR (Nikon D70s) as above. Results: Power density decreased significantly as the layer thickness of the infection control barrier increased, except the one-fold and two-fold in halogen light curing unit. Especially, when the barrier was four-fold and more in the halogen light curing unit, the decrease of power density was more prominent. The wavelength of light curing units was not affected by the barriers and almost no change was detected in the peak wavelength. Light diffusion of LED light curing unit was not affected by barriers, however, halogen light curing unit showed decrease in light diffusion angle when the barrier was four-fold and statistically different decrease when the barrier was eight-fold (p < 0.05). Conclusions: It could be assumed that the infection control barriers should be used as two-fold rather than one-fold to prevent tearing of the barriers and subsequent cross contamination between the patients.

염산부플로메딜 정과 염산티클로피딘 정의 용출시험법 개발

이륜경(Ryun-Kyung Lee),정경록(Gyeong-Rok Jeong),오현숙(Hyun-Sook Oh),심지연(Jee-Youn Shim),서상철(Sang-Chul Suh),이효정(Hyo-Jung Lee),김민아(Min-A Kim),박성민(Seong-Min Park),이규하(Kyu-Ha Lee),손경희(Kyung-Hee Sohn),김인규(In-Kyu Ki 대한약학회 2012 약학회지 Vol.56 No.4

Drug dissolution test has been used for the purpose of both quality control of solid oral dosage forms and predicting in vivo drug release profiles. In this study, the dissolution profiles of buflomedil hydrochloride tablets and ticlopidine hydrochloride tablets were investigated according to the “Guidelines on Specifications of Dissolution tests for Oral dosage forms” of Korean Pharmacopoeia (KP). The analytical method using HPLC was validated. The validation was performed in terms of specificity, linearity, accuracy, precision and limit of quantitation.

생팥과 증자팥의 성분 및 생리활성 비교

이륜경 ( Ryun Kyung Lee ),김미선 ( Mi Sun Kim ),이예슬 ( Ye Seul Lee ),이만효 ( Man Hyo Lee ),이종화 ( Jong Hwa Lee ),손호용 ( Ho Yong Sohn ) 한국미생물생명공학회(구 한국산업미생물학회) 2014 한국미생물·생명공학회지 Vol.42 No.2

본 연구에서는 팥의 유용기능을 이용한 고부가가치 식품 개발을 목표로 무처리한 생팥 분말과 1차 자숙 후 고온 증자하고 동결건조하여 제조된 식용 팥 분말을 대상으로 에탄올 추출물을 조제하고, 각각의 유용성분 및 항산화, 항균, 항당뇨, 및 항혈전 활성을 평가하였다. 추출효율의 경우 생팥보다 증자팥이 약 1.2배 증가되었으며, 추출물의 총폴리페놀 함량도 증자팥이 1.2배 증가되었다. 그러나, 증자팥의 총플라보노이드 함량은 생팥 대비 30% 수준으로 감소하였으며, 총당 및 환원당 함량 역시 생팥 대비 27.9% 및 30.8% 수준으로 감소하였다. 항산화 활성은 생팥 및 증자팥에서 모두 우수한 음이온 및 양이온 소거능을 나타내었으나, 생팥이 증자팥보다 강력하였으며, 환원력 및 nitrite 소거능에서도 생팥 추출물이 우수하였다. 한편 α-amylase 저해활성은 생팥에서 우수하였으며, α-glucosidase 저해활성은 증자팥에서 상대적으로 우수하였다. 가장 특이한 활성은 항혈전 활성 평가에서 확인되었으며, 생팥 추출물은 매우 강력한 프로트롬빈 및 혈액응고인자 저해를 나타낸 반면 증자팥 추출물에서 는 거의 활성이 나타나지 않았다. 상기 결과는 생팥의 증자과정 중 유용성분의 소실 및 유용활성의 손실이 나타남을 의미하며, 향후 생팥의 적합한 삶기 공정, 열처리 및 건조공정의 개발이 필요하며, 특히 현재까지 대부분 폐기되고 있는 증자팥 제조단계의 팥 자숙액의 효율적인 이용에 대한 연구가 필요함을 제시하고 있다. 본 연구결과는 기능성 팥 음료 및 양갱 제조 등을 위한 팥 고부가가치 식품 개발 기본자료로 활용될 것이다. In the course of study for the development of functional food using red beans (azuki beans, Phaseolus radiatus L.), the ethanol extracts from raw-red bean (RRB) and boiled-red bean (BRB) were prepared, and the components and various biological activities of both were compared. It was observed that the extraction yield, and the total polyphenol content, of the BRB were 1.2 times higher than that of the RRB. However, the contents of total flavonoid, total sugar and reducing sugar in the BRB were 30, 27.9 and 30.8% respectively when compared with those of RRB. In relation to antioxidative activity, both RRB and BRB exhibited moderate DPPH anion, ABTS cation, and nitrite scavenging activities and reducing power, though in all cases RRB demonstrated stronger activities than BRB. The extracts of RRB and BRB did not reveal any antimicrobial activities. In a α- amylase inhibitory activity assay, RRB was higher than BRB, while BRB showed higher α-glucosidase inhibitory activity than RRB. A strong and particular activity was observed in an anti-thrombosis activity assay of RRB. The extract of RRB demonstrated strong inhibitions against prothrombin and blood coagulation factors, with moderate thrombin inhibition. However, the extract of BRB did not exhibit any significant anti-thrombosis activity. Our results indicate that RRB has different, but useful biological activities, and loss or elimination of the biologically active substances in RRB occurs during the production of BRB. Therefore, to develop more functional foods from red beans, a study of suitable boiling, heating and drying processes is essential, and the efficient re-use of boiled waste water from the boiling process is necessary. These results could be applied to the further development of functional red bean beverages and sweat red bean pastes.

브롬화옥틸로늄 정과 브롬화피나베륨 정의 용출규격 설정

이륜경(Ryun-Kyung Lee),이윤애(Yoon-Ae Lee),심지연(Jee-Youn Shim),김민아(Min-A Kim),손경희(Kyung-Hee Sohn),박혜림(Hye-Rim Park),김희성(Hee-Sung Kim),송영미(Young-Mi Song),이수정(Su-Jung Lee),사홍기(Hong-Kee Sah),최후균(Hoo-Kyun Choi) 대한약학회 2011 약학회지 Vol.55 No.5

To secure the good quality of pharmaceutical products, dissolution specifications for Octylonium bromide tablets and Pinaverium bromide tablets are needed to be established, which are enrolled in KPC (Korea Pharmaceutical Codex) with having no appropriate specifications. For establishing dissolution specifications, a number of experiments based on the“Guideline of Dissolution Testing for Solide Oral Dosage Forms” were performed. The results of this study will be used for revising KPC and it is expected to contribute to the incessant production of quality ensured drugs.

유통 축·수산물 중 잔류벤질페니실린의 검출 및 모니터링

이효정(Hyo Jeong Lee),강영원(Young Won Kang),이수민(Soo Min Lee),안경아(Kyung A An),이륜경(Ryun Kyung Lee),서상철(Sang Cheol Seo),이주희(Ju Hee Lee),임무혁(Moo Hyeog Im),이정림(Jeong Rim Lee),홍충만(Choong Man Hong),장문익(Moon Ik Ch 한국식품과학회 2014 한국식품과학회지 Vol.46 No.3

국내 유통 중인 돼지고기, 소고기, 닭고기, 넙치 232건의 시료에 대해 벤질페니실린의 잔류량을 조사하기 위해 현행 식품공전 상 축·수산물 중 벤질페니실린 분석법에 대한 검증을 실시하고 모니터링을 수행하였다. 그 결과 벤질페니실린에 대한 분석법은 CODEX에서 요구하는 기준에 적합한 정밀성과 정확성을 보였다. 벤질페니실린의 잔류량 모니터링 결과 축산물 193건 중 11건, 수산물 39건 중 2건 총 13건이 검출되었다. 검출된 수준은 축·수산물 12건에서 0.001-0.004 mg/kg으로 잔류허용기준 이하로 검출되었으나, 축산물 중 국내산 돼지고기 1건에서 잔류허용기준을 초과한 0.134 mg/kg 수준으로 검출되어 해당 유관기관에 부적합 통보 및 사후관리를 요청하였다. 따라서 벤질페니실린 등 항생제는 다른 동물용 의약품에 비해 사용빈도가 많은 만큼 지속적으로 잔류모니터링과 관리가 필요할 것으로 판단된다. Penicillin and its salts, including the benzatine, procaine, and sodium salts, have been widely used in human and veterinary medicine. Owing to their low toxicity, they currently form the most important group of antibiotics. However, overdose and abuse of these antibiotics may lead to potential risk in human health. Therefore, this study was conducted to validate the analysis method established by the Korea Food Code in 2012 and to monitor the levels of benzylpenicillin residues in products with reference to the maximum residue level (MRL). Of the 232 product samples tested, benzylpenicillin was detected in 11 livestock products and 2 marine products. Benzylpenicillin concentrations were found to be lower than the MRL in 12 products; however, the concentration of benzylpenicillin was found to be greater than the MRL in 1 pork product. The limit of quantification (LOQ) for benzylpenicillin was found to be 0.001-0.002 mg/kg, with an average recovery of 90.4-115.3%. Calibrations showed good linearity of 0.995 over a range of 0.002-0.05 mg/kg.