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Effect of Supplementary Dietary L-carnitine and Yeast Chromium on Lipid Metabolism of Laying Hens
Du, Rong,Qin, Jian,Wang, Jundong,Pang, Quanhai,Zhang, Chunshan,Jiang, Junfang Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.2
Two hundred and eighty-eight 21-week-old Hyline Brown laying hens were randomly allotted to 9 treatments, 32 birds for each treatment. A 3${\times}$3 (chromium${\times}$L-carnitine) factorial experiment was designed to investigate the single and interactive effects of adding yeast chromium (0, 400 and 600 ${\mu}g/kg$) and L-carnitine (0, 50 and 100 mg/kg) to corn-soybean diets on lipid metabolism of laying hens for 7 weeks. The results showed that 600 ${\mu}g/kg$ chromium or 100 mg/kg L-carnitine had significant effects on most indices of lipid metabolism (p<0.05 or 0.01). There were significant interactions on the concentration of liver triglycerides, egg yolk cholesterol, abdominal fat percentage between chromium and L-carnitine (p=0.0003-0.0500). Adding 400 ${\mu}g/kg$ chromium and 100 mg/kg Lcarnitine simultaneously was the best for reducing egg yolk cholesterol and adding 400 ${\mu}g/kg$ chromium and 50 mg/kg L-carnitine at the same time was the best for reducing abdominal fat percentage. There was no side effect on production performance of laying hens while chromium or (and) L-carnitine reduced liver lipid, abdominal fat and egg yolk cholesterol.
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Impacts of espoused national cultural values on innovation in IT-enabled global sourcing
( Rong Du ) 한국감성과학회 2017 한국감성과학회 국제학술대회(ICES) Vol.2017 No.-
In this study, we explored the different roles that knowledge sharing and exploitative learning play in employees’ innovative behavior, and investigated the different moderating effects of employees’ espoused national cultural values on the relationship between exploitative learning and innovative behavior in the Chinese IT-enabled global service companies with different ownerships. We proposed a theoretical model to characterize these antecedents of innovative behavior. A structured research survey was conducted and data were collected from a sample of 484 full-time employees in 3 IT-enabled global service-provisioning companies in China. Results indicate that knowledge sharing is positively associated with innovative behavior in multinational corporations and private IT-enabled global service; espoused power distance has a significant positive moderating effect on exploitative learning-innovative behavior relationship in state-owned and private firms; espoused collectivism has a significant moderating effect only in state-owned firms in China.
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Some Motifs Were Important for Myostatin Transcriptional Regulation in Sheep (Ovis aries)
Du, Rong,An, Xiao-Rong,Chen, Yong-Fu,Qin, Jian Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.4
Many motifs along the 1.2 kb myostatin promoter (MSTNpro) in sheep have been found by the MatInspecter program in our recent study. To further verify the role of the motifs and better understand the transcriptional regulation mechanism of the myostatin gene in sheep, the reporter gene EGFP (enhanced green fluorescent protein) was selected and the wild-type (W) vector MSTNPro$^W$-EGFP or motif-mutational (M) vector MSTNPro$^M$-EGFP were constructed. The transcriptional regulation activities were analyzed by detecting the fluorescence strength of EGFP in C2C12 myoblasts transfected with the vectors. The results showed that E-box (E) 3, E4, E5 and E7, particularly E3, E5 and E7, had important effects on the activity of the 1.2 kb sheep myostatin promoter. In addition, we also detected several other important motifs such as MTBF (muscle-specific Mt binding factor), MEF2 (myocyte enhancer factor 2), GRE (glucocorticoid response elements) and PRE (progesterone response elements) along the sheep myostatin promoter by the mutational analysis.
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두려영 ( Du Li-rong ) 중국어문연구회 2017 中國語文論叢 Vol.0 No.80
There are different explanations of Que(闕) of Pang(旁) in Shuowenjiezi(說文解字). Scholars not only had different understanding of the cause of its creation and different interpretations of its definition, but also differed greatly in the explanations of Que(闕) and in supplement to it since Song Dynasty. Some scholars consider that Que(闕)originates from Shuowenjiezi(≪說文解字≫). For instance, Xu, Kai(徐?)in Southern Tang believes that without teacher`s explanation of `Cong Shang`(從上)of Pang(旁), Xu, Shen(許愼) notes Que(闕). Duan, Yucai(段玉裁) and Wang, Yun(王筠) in Qing Dynasty believe that Xu, Shen(許愼)is not konwing well “□” in Pang(旁)causes using Que(闕); while others such as Yan, Kejun(嚴可均) and Yao, Wentian(姚文田) in Qing Dynasty consider that Que(闕) does not originate from Shuowenjiezi(≪說文解字≫). It is added by later scholars. They analyze that Que(闕) is added for the lost part in Shuowenjiezi(≪說文解字≫) by collators. Based on the form of Pang(旁) on bones or tortoise shells of the Shang Dynasty and ancient bronze objects,and Chinese character studies of“tongyi”(同意), this article sorts out the intention of its creation and evolution process of Pang`s(旁) form and then analyzes the origin of Que(闕) of Pang(旁). From the former research, it is believed that the Que (闕) of Pang(旁) is caused by the gradual change of the forms of Chinese characters, and created by Xu, Shen(許愼).
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The Dust Deposition Process and Biogeochemical Impacts in the Northern South China Sea
Du Shuhuan,Ariful Islam G. M.,Xiang Rong,Yang Xiaoli 한국기상학회 2021 Asia-Pacific Journal of Atmospheric Sciences Vol.57 No.1
Modern dust plays essential roles in marine sediments, providing nutrient and crucial elements for primary productivity and aquatic ecosystems. However, present marine atmospheric dust observational evidence concerning the biogeochemical response to dust deposition process is spared for a long time. Here a total of 147 aerosols samples were collected near the northern South China Sea (SCS) coast between 13 February, 2016, and 12 February, 2017, have been investigated for the grain size distribution, depositional flux, ten-day back trajectories, clusters analysis, combining with primary productivity (Chlorophyll-a concentration) to indicate the present-day dust deposition process and biogeochemical impacts in the northern SCS on the first time. Our results illustrate the deposition flux and grain size distribution change in different season, grain size distribution in winter are coarser particles than that in the summer, while the maximum and minimum dry deposition flux are 12 December, 2016, (60.4 mg m−2 d−1) and 26 August, 2016, (5 mg m−2 d−1), respective. In addition, the back trajectories and three clusters analyze results indicating 53% of air masses originating locally near the coastal areas of China from the marine boundary layer (0–0.5 km) in the summer monsoon, while 48% dust from the northeast wind is prevailing during the winter monsoon over a relatively higher level (0–1.5 km). Furthermore, an apparent Chl-a blooming after aeolian dust input with a period of 1–2 days in the SCS. Therefore, atmospheric dust contains readily bioavailable iron and enhance the biogeochemical impact in the oligotrophic SCS.
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Blocking Bcl-2 Leads to Autophagy Activation and Cell Death of the HEPG2 Liver Cancer Cell Line
Du, Peng,Cao, Hua,Wu, Hao-Rong,Zhu, Bao-Song,Wang, Hao-Wei,Gu, Chun-Wei,Xing, Chun-Gen,Chen, Wei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10
Background: Apoptosis may be induced after Bcl-2 expression is inhibited in proliferative cancer cells. This study focused on the effect of autophagy activation by ABT737 on anti-tumor effects of epirubicin. Methods: Cytotoxic effects of ABT737 on the HepG2 liver cancer cell line were assessed by MTT assay and cell apoptosis through flow cytometry. Mitochondrial membrane potential was measured by fluorescence microscopy. Monodansylcadaverin (MDC) staining was used to detect activation of autophagy. Expression of p53, p62, LC3, and Beclin1, apoptotic or autophagy related proteins, was detected by Western blotting. Results: ABT737 and epirubicin induced growth inhibition in HepG2 cells in a dose- and time-dependent manner. Both ABT737 and epirubicin alone could induce cell apoptosis with a reduction in mitochondrial membrane potential as well as increased apoptotic protein expression. Further increase of apoptosis was detected when HepG2 cells were co-treated with ABT373 and epirubicin. Furthermore, our results demonstrated that ABT373 or epirubicin ccould activate cell autophagy with elevated autophagosome formation, increased expression of autophagy related proteins and LC3 fluorescent puncta. Conclusions: ABT737 influences cancer cells through both apoptotic and autophagic mechanisms, and ABT737 may enhance the effects of epirubicin on HepG2 cells by activating autophagy and inducing apoptosis.
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Gene Mapping Related to Yellow Green Leaf in a Mutant Line in Rice (Oryza sativa L.)
Peng Du,Ying Hua Ling,Xian Chun Sang,Fang Ming Zhao,Rong Xie,Zheng Lin Yang,Guang Hua He 한국유전학회 2009 Genes & Genomics Vol.31 No.2
A mutant, which derived from the restorer line Jinhui10 treated with EMS, showed completely yellow green leaves, and it had low chlorophyll content and poor agronomic characteristics during the growing stage. The F1 plants from the cross between normal × the mutant showed normal green leaves, and the segregation ratio of normal to yellow green leaves was 3:1 in F2 population. It indicated that the trait was controlled by a single recessive nuclear gene, temporarily designated as ygl3. The gene ygl3 was mapped between RM468 and RM3684 with genetic distances 8.4 cM and 1.8 cM on chromosome 3. This result would be used as genetic information for fine mapping and map-based cloning of ygl3 gene.
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Effect of Yeast Chromium and L-carnitine on Lipid Metabolism of Broiler Chickens
Wang, Jundong,Du, Rong,Qin, Jian,Wang, Shaolin,Wang, Wenkui,Li, Hongquan,Pang, Quanhai Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.12
A $3{\times}4$ (chromium and L-carnitine) experiment was designed to investigate the single and interactive effects of adding yeast Cr and L-carnitine to corn-soybean meal diets on lipid metabolism of broiler chickens. Four hundred and eighty one-day-old avian chickens were randomly allocated to 12 treatments of 40 each for 7 weeks. Levels of adding Cr were 0, 400, $600{\mu}g/kg$ and those of Lcarnitine was 0, 30, 50, 100 mg/kg, respectively. The result showed that adding $600{\mu}g/kg$ Cr or 100 mg/kg L-carnitine alone had better regulative effects on fat and cholesterol metabolism than lower adding levels. Effects were more significant at the end of the experiment. There were significantly interactive effects between Cr and L-carnitine on triaclyglycerol, whole cholesterol, HDL, dissociating FFA, and blood glucose, cholesterol and triaclyglycerol of liver, and cholesterol of chest muscle at the end of experiment (p=0.0001-0.0315). But Cr or L-carnitine had no significant effect on growth performance of broiler chickens (p>0.05).
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